• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Mechanism of Coupling ATP hydrolysis to Ca^<2+> transport in sarcoplasmic reticulum Ca^<2+>-ATPase

Research Project

Project/Area Number 14580619
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Structural biochemistry
Research InstitutionAsahikawa Medical College

Principal Investigator

DAIHO Takashi  Asahikawa Medical College, Biochemistry, Assistant professor, 医学部, 助教授 (90207267)

Project Period (FY) 2002 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2003: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 2002: ¥2,200,000 (Direct Cost: ¥2,200,000)
Keywordssarcoplasmic reticulum / Ca^<2+>-ATPase / phosphorylated intermediate / Daner's disease / Active transport / SERCA / calcium pump / P-type ATPase / P-type ATPase / ATPase / ドメイン間相互作用 / 界面活性剤 / 削除変異 / loop
Research Abstract

1.Mg/F/E(Enzyme) complex of sarcoplasmic reticulum Ca^<2+>-ATPase is an stable analog of phosphorylated intermediate(EP). We have found that the solubilized and delipidated Mg/F/E is remarkably stable against denaturation. 2.The Ca^<2+>-ATPase has 3 cytoplasmic domains (A,P,N). We have found that interaction between Val^<200> loop of A domain and P domain is important for Ca^<2+>-translocation from EP.3.Glu40-Ser48 loop connect A domain and the 1st transmembrane segment. We have found that the adequate length of this loop is important for conformational transition of EP, which is critical for Ca^<2+> translocation. We found that some mutations in this loop region reported in the Darier's disease pedigrees will also cause inhibition of EP transition. 4.We have found that Arg^<334> is important for EP transition and Tyr^<122> for Ca^<2+> translocation after the transition of EP. We have proposed a model for coupling, in which energy of ATP hydrolysis is transmitted to Ca^<2+> transport site through the large movements of cytoplasmic domains. 5.We have shown that the complexes Be/F/E,Al/F/E, and Mg/F/E are stable analogs for the ground state, transition state, and enzyme/product in EP hydrolysis. We also found that the structural changes in the catalytic site in EP hydrolysis will close the lumenal gate for Ca^<2+> translocation to prevent Ca^<2+> leak form the lumen.

Report

(3 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • Research Products

    (29 results)

All Other

All Publications (29 results)

  • [Publications] Kazuo Yamasaki, Takashi Daiho, Hiroshi Suzuki: "Remarkable stability of solubilized and delipidated sarcoplasmic reticulum Ca^<2+>-ATPase with tightly bound fluoride and magnesium against detergent induced denaturation"The Journal of Biological Chemistry. 277・16. 13615-13619 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 山崎 和生, 大保 貴嗣, 鈴木 裕: "筋小胞体Ca^<2+>-ATPaseの細胞質ドメインに存在するイオンペアの役割"生化学. 74・8. 1023 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 大保 貴嗣, 山崎 和生, 王 国麗, Danko Stefania, 鈴木 裕: "筋小胞体Ca^<2+>-ATPaseのAドメイン-膜貫通ヘリックス連結領域の部位特異的変異"生化学. 74・8. 1023 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Sanae Kato, Mika Kamidochi, Takashi Daiho, Kazuo Yamasaki, Wang Gouli, Hiroshi Suzuki: "Val^<200> residue in Lys^<189>-Lys^<205> outermost loop on the A domain of sarcoplasmic reticulum Ca^<2+>-ATPase is critical for rapid processing of phosphoenzyme intermediate after loss of ADP sensitivity"The Journal of Biological Chemistry. 278・11. 9624-9629 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yamasaki Kazuo, Daiho Takashi, Suzuki Hiroshi: "Roles of ionic interactions between P and N domains of sarcoplasmic reticulum Ca^<2+>-ATPase"生化学. 75・8. 965 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Suzuki Hiroshi, Yamasaki Kazuo, Daiho Takashi, Miyauchi Yuki, Iizuka Hajime, Danko Stefania: "Structure and function of Ca^<2+> pump and the molecular basis of genetic diseases caused by its mutations"生化学. 75・8. 762 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Daiho Takashi, Yamasaki Kazuo, Danko Stefania, Suzuki Hiroshi: "Roles and mechanism of domain movements and interactions in the Ca^<2+> pump"生化学. 75・8. 774 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Takashi Daiho, Kazuo Yamasaki, Guoli Wang, Stefania Danko, Hajime Iizuka, Hiroshi Suzuki: "Deletions of Any Single Residues in Glu^<40>-Ser^<48> Loop Connecting A Domain and the First Transmembrane Helix of Sarcoplasmic Reticulum Ca^<2+>-ATPase Result in Almost Complete Inhibition of Conformational Transition and Hydrolysis of Phosphoenzyme Intermediate"The Journal of Biological Chemistry. 278・40. 39197-39204 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kazuo Yamasaki, Takashi Daiho, Stefania Danko, Hiroshi Suzuki: "Multiple and distinct effects of mutations of Tyr^<122>, Glu^<123>, Arg^<329>, and Arg^<334> involved in interactions between the top part of second and fourth transmembrane helices in Sarcoplasmic Reticulum Ca^<2+>-ATPase CHANGES IN CYTOPLASMIC DOMAIN ORGANIZATION DURING ISOMETRIC TRANSITION OF PHOSPHOENZYME INTERMEDIATE AND SUBSEQUENT Ca^<2+> RELEASE"The Journal of Biological Chemistry. 279・3. 2202-2210 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Stefania Danko, Kazuo Yamasaki, Takashi Daiho, Hiroshi Suzuki: "Distinct Natures of Be/F-bound, AI/F-bound, and Mg/F-bound Stable Analogues of ADP-insensitive Phosphoenzyme Intermediate of Sarcoplasmic Reticulum Ca^<2+>-ATPase : Changes in catalytic and transport sites during phoshoenzyme hydrolysis"The Journal of Biological Chemistry. 279・15. 14991-14998 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kazuo Yamasaki, et al.: "Remarkable stability of solubihzed and delipidated sarcoplasmic reticulum Ca^<2+>-ATPase with tightly bound fluoride and magnesium against detergent-induced denaturation"The Journal of Biological Chemistry. 277-16. 13615-13619 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Sanae Kato, et al.: "Val^<200> residue in Lys^<189>-Lys^<205> outermost loop on the A domain of sarcoplasmic reticulum Ca^<2+>-ATPase is critical for rapid processing ofphosphoenzyme intermediate after loss of ADP sensitivity"The Journal of Biological Chemistry. 278-11. 9624-9629 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Takashi Daiho, et al.: "Deletions of any single residues in Glu^<40>-Ser^<48> loop connecting a domain and the first transmembrane helix of sarcoplasmic reticulum Ca^<2+>-ATPase result in almost complete inhibition of conformational transition and hydrolysis of phosphoenzyme intermediate"The Journal of Biological Chemistry. 278-40. 39197-39204 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kazuo Yamasaki, et al.: "Multiple and distinct effects of mutations of Tyr^<122>, Glu^<123> Arg^<324>, and Arg^<334> involved in interactions between the top part of second and fourth transmembrane helices in Sarcoplasmic Reticulum Ca^<2+>-ATPase CHANGES IN CYTOPLASMIC DOMAIN ORGANIZATION DURING ISOMETRIC TRANSITION OF PHOSPHOENZYME INTERMEDIATE AND SUBSEQUENT Ca^<2+> RELEASE"The Journal of Biological Chemistry. 279-3. 2202-2210 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Stefania Danko, et al.: "Distinct Natures of Be/F-bound, Al/F-bound, and Mg/F-bound Stable Analogues of ADP-msensitive Phosphoenzyme Intermediate of Sarcoplasmic Reticulum Ca^<2+>-ATPase : Changes in catalytic and transport sites during phoshoenzyme hydrolysis"The Journal of Biological Chemistry. 279. 14991-14998 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kazuo Yamasaki, Takashi Daiho, Hiroshi Suzuki: "Remarkable stability of solubilized and delipidated sarcoplasmic reticulum Ca^<2+>-ATPase with tightly bound fluoride and magnesium against detergent-induced denaturation"The Journal of Biological Chemistry. 277・16. 13615-13619 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] 山崎 和生, 大保 貴嗣, 鈴木 裕: "筋小胞体Ca^<2+>-ATPaseの細胞質ドメインに存在するイオンペアの役割"生化学. 74・8. 1023 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] 大保 貴嗣, 山崎 和生, 王 国麗, Danko Stefania, 鈴木 裕: "筋小胞体Ca^<2+>-ATPaseのAドメイン-膜貫通ヘリックス連結領域の部位特異的変異"生化学. 74・8. 1023 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] Sanae Kato, Mika Kamidochi, Takashi Daiho, Kazuo Yamasaki, Wang Gouli, Hiroshi Suzuki: "Val^<200> residue in Lys^<189>-Lys^<205> outermost loop on the A domain of sarcoplasmic reticulum Ca^<2+>-ATPase is critical for rapid processing of phosphoenzyme intermediate after loss of ADP sensitivity"The Journal of Biological Chemistry. 278・11. 9624-9629 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Yamasaki Kazuo, Daiho Takashi, Suzuki Hiroshi: "Roles of ionic interactions between P and N domains of sarcoplasmic reticulum Ca^<2+>-ATPase"生化学. 75・8. 965 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Suzuki Hiroshi, Yamasaki Kazuo, Daiho Takashi, Miyauchi Yuki, Iizuka Hajime, Danko Stefania: "Structure and function of Ca^<2+> pump and the molecular basis of genetic diseases caused by its mutations"生化学. 75・8. 762 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Daiho Takashi, Yamasaki Kazuo, Danko Stefania, Suzuki Hiroshi: "Roles and mechanism of domain movements and interactions in the Ca^<2+> pump"生化学. 75・8. 774 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Takashi Daiho, Kazuo Yamasaki, Guoli Wang, Stefania Danko, Hajime Iizuka, Hiroshi Suzuki: "Deletions of any single residues in Glu^<40>-Ser^<48> loop connecting a domain and the first transmembrane helix of sarcoplasmic reticulum Ca^<2+>-ATPase result in almost complete inhibition of conformational transition and hydrolysis of phosphoenzyme intermediate"The Journal of Biological Chemistry. 278・40. 39197-39204 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kazuo Yamasaki, Takashi Daiho, Stefania Danko, Hiroshi Suzuki: "Multiple and distinct effects of mutations of Tyr^<122>, Glu^<123>, Arg^<324>, and Arg^<334> involved in interactions between the top part of second and fourth transmembrane helices in Sarcoplasmic Reticulum Ca^<2+>-ATPase CHANGES IN CYTOPLASMIC DOMAIN ORGANIZATION DURING ISOMETRIC TRANSITION OF PHOSPHOENZYME INTERMEDIATE AND SUBSEQUENT Ca^<2+> RELEASE"The Journal of Biological Chemistry. 279・3. 2202-2210 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Stefania Danko, Kazuo Yamasaki, Takashi Daiho, Hiroshi Suzuki: "Distinct Natures of Be/F-bound, Al/F-bound, and Mg/F-bound Stable Analogues of ADP-insensitive Phosphoenzyme Intermediate of Sarcoplasmic Reticulum Ca^<2+>-ATPase : Changes in catalytic and transport sites during phoshoenzyme hydrolysis"The Journal of Biological Chemistry. 279(In press). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kazuo Yamasaki, Takashi Daiho, Hiroshi Suzuki: "Remarkable Stability of Solubilized and Delipidated Sarcoplasmic Reticulum Ca^<2+>-ATPase with Tightly Bound Fluoride and Magnesium against Detergent-induced Denaturation"THE JOURNAL OF BIOLOGICAL CHEMISTRY. 277・16. 13615-13619 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 山崎 和生, 大保 貴嗣, 鈴木 裕: "筋小胞体Ca^<2+>-ATPaseの細胞質ドメインに存在するイオンペアの役割"生化学. 74・8. 1023 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 大保 貴嗣, 山崎 和生, 王 国麗, Danko Stefania, 鈴木 裕: "筋小胞体Ca^<2+>-ATPaseのAドメイン-膜貫通ヘリックス連結領域の部位特異的変異"生化学. 74・8. 1023 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Sanae Kato, Mika Kamidochi, Takashi Daiho, Kazuo Yamasaki, Wang Gouli, Hiroshi Suzuki: "Val^<200> Residue in Lys^<189>-Lys^<205> Outermost Loop on A Domain of Sarcoplasmic Reticulum Ca^<2+>-ATPase Is Critical for Rapid Processing of Phosphoenzyme Intermediate after Loss of ADP Sensitivity"THE JOURNAL OF BIOLOGICAL CHEMISTRY. 278・11. 9624-9629 (2003)

    • Related Report
      2002 Annual Research Report

URL: 

Published: 2002-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi