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REGULATORY MECHANISM OF PHOSPHOLIPASE D IN CELL GROWTH AND SURVIVAL SIGNALING

Research Project

Project/Area Number 14580646
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Functional biochemistry
Research InstitutionGIFU UNIVERSITY

Principal Investigator

BANNO Yoshiko  GIFU UNIVERSITY, SCHOOL OF MEDICINE, ASSOCIATE PROFESSOR, 医学部, 助教授 (50116852)

Co-Investigator(Kenkyū-buntansha) NOZAWA Yoshinori  GIFU INTERNATIONAL INSTITUTE, CHIEF, 所長兼部長 (10021362)
NAKASHIMA Shigeru  GIFU UNIVERSITY, SCHOOL OF MEDICINE, PROFESSOR, 医学部, 教授 (60188935)
Project Period (FY) 2002 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2003: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2002: ¥2,200,000 (Direct Cost: ¥2,200,000)
KeywordsPHOSPHOLIPASE D / PHOSPHATIDYLINOSITOL 3-KINASE / SURVIVAL SIGNALING / AKT / ERK / SPHINGOSINE 1-PHOSPHATE / 膜脂質変換酵素 / 細胞増殖
Research Abstract

Phospholipase D has been known to be important signaling enzyme to regulate cell proliferation and survival. Two types of PLD(1,2) have been cloned in mammalian tissues and cells. The PLD1 and PLD2 are differently regulated and distributed in the cells. Sphingosine 1-phosphate(S1P) stimulation of S1P_3-overexpressing CHO cells induced activation of PLD, PI3-kinase, and Akt. S1P-induced activation of PI3-kinase and Akt was abrogated by 1-butanol, which inhibited phosphatidic acid(PA) formation by PLD. Overexpression of the wild-type PLD1,2 resulted in increased activation of Akt, ERK and p70S6 kinase in response to S1P. Actinomycine D(AchD) induced apoptosis in CHO cells, and the apoptosis was prevented by overexpression of PLD1,2. The treatment of CHO cells with AchD induced activation of survival signaling(PI3K, Akt, ERK, p70S6K) at earlier times of apoptosis. The activities of survival signaling were increased by overexpression of PLD1,2. These results demonstrate that PLD participates in the potentiation of survival signaling activation. We have found that PLD1 play a role in melanogenesis of mouse B16 melanoma cells. It has been shown that PLD1 negatively regulated the melanogenic signaling by modulating the expression of tyrosinase in B16 melanoma cells.

Report

(3 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • Research Products

    (41 results)

All Other

All Publications (41 results)

  • [Publications] Tan, Z.et al.: "Involvement of the mitogen-activated protein kinase family in the tetracaine-induced PC12 cell death"Anesthesiology. 96. 1191-1201 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Akao, Y. et al.: "Ceramide accumulation is independent of camptothecin-induced apoptosis in prostatae cancer LNCaP cells"Biochem.Biophys.Res.Commun.. 294. 363-370 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Tin, Y.et al.: "Inhibitory effect of local anesthetics on bradykinin-induced phospholipase D in rat pheochromocytoma PC12 cells."Chin.Anes.Analg.. 95. 88-97 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Wang, S. et al.: "Two forms of membrane-bound sphingosine kinase in Tetrahymena and activity changes during growth and the cell cycle."J.Euk.Microbiol.. 49. 305-311 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Osawa, Y. et al.: "Tumor necrosis factor alpha-induced interleukin-8 production via NF-kappaB and phosphatidylinositol 3-kinase/Akt pathways inhibits cell apoptosis in Human hepatocytes"Infect.Immun.. 70. 6294-6301 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Banno, Y. et al.: "Involvement of phospholipase D in insulin-like growth factor-I- induced activation of ERK, but not phosphatidylinositol 3-kinase or Akt, in Chinese Hamster ovary cells"Biochem.J.. 369. 363-368 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yamada Y. et al.: "Association of a polymorphism of the phospholipase D2 gene with the prevalence of cotorectal cancer."J.Mol.Med. 81. 126-131 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Osawa, Y. et al.: "Expression of the NF-κB target gene X-Ray-inducible immediate early response factor-1 short enhances TNF-α-induced hepatocyte apoptosis by inhibiting Akt activation."J.Immunol.. 170. 4056-4060 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Banno, Y. et al.: "Hydrogen peroxide-induced phospholipase D activation and its PKC dependence are modulated by pH changes in PC12 cells"Biochem.Biophys.Res.Commun.. 312. 1087-1093 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Sagawa, H.: "Gelsolin suppresses tumorigenicity through inhibiting PKC activation in a human lung cancer cell line, PC10."Br.J.Cancer. 88. 606-612 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Nakade, Y. et al.: "Regulation of Sphingosine Kinase 1 Gene Expression by Protein Kinase C in a Human Leukemia Cell Line, MEG-O1."Biochim.Biophys.Acta. 1635. 104-116 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Terada, N. et al.: "Compartmentation of the mouse cerebellar cortex by sphingosine kinase."J.Comp.Neurol.. 469. 119-127 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ohguchi, K. et al.: "Involvement of Phospholipase D1 in Melanogenesis of Mouse B16 Melanoma Cells."J.Biol.Chem.. 279. 3408-3412 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yamada M. et al.: "Overexpression of phospholipase D prevents actinomycin D-induced apoptosis through potentiation of phosphoinositide 3-kinase signaling pathways in CHO cells."Biochem.J.. 378. 649-656 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Tan, Z.: "Involvement of the mitogen-activated protein kinase family in the tetracaine-induced PC12 cell death."Anesthesiology. 96. 1191-1201 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Akao, Y.: "Ceramide accumulation is independent of camptothecin-induced apoptosis in prostatae cancer LNCaP cells."Biochem.Biophys.Res.Commun.. 294. 363-370 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Tin, Y.: "Inhibitory effect of local anesthetics on bradykinin-induced phospholipase D in rat pheochromocytoma PC12 cells."Chin.Anes.Analg.. 95. 88-97 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Wang, S.: "Two forms of membrane-bound sphingosine kinase in Tetrahymena and activity changes during growth and the cell cycle."J.Euk.Microbiol.. 49. 305-311 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Osawa, Y.: "Tumor necrosis factor alpha-induced interleukin-8 production via NF-kappaB and phosphatidylinositol 3-kinase/Akt pathways inhibits cell apoptosis in Human hepatocytes"Infect.Immun.. 70. 6294-6301 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Banno, Y.: "Involvement of phospholipase D in insulin-like growth factor-I-induced activation of ERK, but not phosphatidylinositol 3-kinase or Akt, in Chinese Hamster ovary cells."Biochem.J.. 369. 363-368 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yamada Y.: "Association of a polymorphism of the phospholipase D2 gene with the prevalence of colorectal cancer."J.Mol.Med.. 81. 126-131 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Osawa, Y.: "Expression of the NF-κB target gene X-Ray-inducible immediate early response factor-1 short enhances TNF-α-induced hepatocyte apoptosis by inhibiting Akt activation."J.Immunol.. 170. 4053-4060 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Banno, Y.: "Hydrogen peroxide-induced phospholipae D activation and its PKC dependence are modulated by pH changes in PC12 cells."Biochem.Biophys.Res.Commun.. 312. 1087-1093 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Sagawa, H.: "Gelsolin suppresses tumorigenicity through inhibiting PKC activation in a human lung cancer cell line, PC1O."Br.J.Cancer. 88. 606-612 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Nakade, Y.: "Regulation of Sphingosine Kinase 1 Gene Expression by Protein Kinase C in a Human Leukemia Cell Line, MEG-01."Biochim.Biophys.Acta.. 1635. 104-116 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Terada, N.: "Compartmenttation of the mouse cerebellar cortex by sphingosine kinase."J.Comp.Neurol.. 469. 119-127 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ohguchi, K.: "Involvement of Phospholipase D1 in Melanogenesis of Mouse B16 Melanoma Cells."J.Biol.Chem.. 279. 3408-3412 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yamada, M.: "Overexpression of phospholipase D prevents actinomycin D-induced apoptosis through potentiation of phosphoinositide 3-kinase signaling pathways in Chinese hamster ovary cells."Biochem.J.. 378. 649-656 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Banno, Y. et al.: "Involvement of Phospholipase D in insulin-like growth factor-I-Induced activation of ERK, but not phosphatidylinositol 3-kinase or Akt, in Chinese Hamster ovary cells"Biochem.J.. 369. 363-368 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Yamada Y. et al.: "Association of a polymorphism of the phospholipase D2 gene with the prevalence of colorectal cancer"J.Mol.Med.. 81. 126-131 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Banno, Y., Nozawa, Y.: "Hydrogen peroxide-induced phospholipae D activation and its PKC dependence are modulated by pH changes in PC12 cells"Biochem.Biophys.Res.Commun.. 312. 1087-1093 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Terada, N. et al.: "Compartmenttation of the mouse cerebellar cortex by sphingosine kinase"J.Comp.Neurol.. 469. 119-127 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Ohguchi, K. et al.: "Involvement of Phospholipase D1 in Melanogenesis of Mouse B16 Melanoma Cells"J.Biol.Cherm.. 279. 3408-3412 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Yamada, M. et al.: "Overexpression of phospholipase D prevents Ach D-induced apoptosls through potentiation of PI 3-klnase signaling pathways in Chinese hamater ovary cells"Biochem.J.. 378. 649-656 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] 坂野喜子, 野澤義則: "生物薬学実験講座"目本薬学会(印刷中). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Chen, J. et al.: "The inhibitory effect of local anesthetics on bradykinin-induced phospholipase D activation in rat pheochromocytoma PC 12 cells"Anesth. Analg.. 95. 88-97 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Osawa, Y. et al.: "Tumor necrosis factor alpha-induced interleukin-8 production via NF-kappaB and PI 3-kinase/Akt pathways inhibits cell apoptosis in Human hepatocytes"Infect. Immun.. 70. 6294-6301 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Wang, S. et al.: "Two forms of membrane-bound sphingosine kinase in Tetrahymena and activity changes during growth and the cell cycle"J. Euk. Microbiol.. 49. 305-311 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Akao, Y. et al.: "Ceramide accumulation is independent of camptothecin-induced apoptosis in prostatae cancer LNCaP cells"Biochem. Biophys. Res. Commun.. 294. 363-370 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Tan, Z.et al.: "Involvement of the mitogen-activated protein kinase family in the tetracaine-induced PC12 cell death"Anesthesiology. 96. 1191-1201 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Banno, Y. et al.: "Involvement of Phospholipase D in insulin-like growth factor-I-induced activation of ERK, but not PI 3-kinase or Akt, in Chinese Hamster ovary cells"Biochem. J.. 369. 363 (2003)

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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