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Identification of epigenetic protein complexes and functional analyses

Research Project

Project/Area Number 14580659
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Functional biochemistry
Research InstitutionNational Institute of Infectious Diseases

Principal Investigator

KATO Hiroyuki  NATIONAL INSTITUTE OF INFECTIOUS DISEASES, DEPARTMENT OF VIROLOGY III, SENIOR RESEARCHER, ウイルス第3部, 主任研究官 (60185866)

Co-Investigator(Kenkyū-buntansha) SHINKAI Yoichi  KYOTO UNIVERSITY, INSTITUTE FOR VIRUS RESEARCH, PROFESSOR, ウイルス研究所, 教授 (20211972)
Project Period (FY) 2002 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2003: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2002: ¥2,700,000 (Direct Cost: ¥2,700,000)
Keywordschromatin / epigenetics / transcription / complex / polycomb / SNF / SWI / G9a / histone methylation
Research Abstract

1.We have isolated a protein, by yeast two-hybrid methods, which specifically binds to human SNF/SWI components hSNF2a/hbrm and hSNF2b/BRG-1. The protein TMF has been reported as a factor that associates and cooperates with androgen receptor. TMF was found to localize in Golgi apparatus. (Mori et al.)
2.We have constructed knock-out mice lacking the G9a gene whose product has methylation activity to lysine 9 of histone H3 (H3K9). The mice show lethality in early development phase and ES cells lacking both alleles of G9a show evidently decreased level of H3K9 methylation of euchromatin region. In vivo transient expression experiments indicated that G9a could play a role in transcription repression. (Tachibana et al.)
3.We have isolated two mammalian polycomb complexes and identified new components. One of the complexes showed methyltransferase activity to histone H3 lysine 27. The other complex had specific binding activity to H3K27. These mutually coordinate activities explain the similarity in genetical phenotypes between these two polycomb complexes and reinforce their importance in transcriptional repression.

Report

(3 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] K.Mori: "A putative nuclear receptor coactivator (TMF/ARA160) associates with hbrm/hSNF2α and BRG-1/hSNF2β and localizes in the Golgi apparatus"FEBS lett.. 520. 127-132 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] M.Tachibana: "C9a histone methyltransferase plays a dominant role in euchromatic histone H3 lysine 9 methylation and is essential for early embryogenesis"Genes & Dev.. 16. 1779-1791 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] M.C.Estable: "MCEF, the newest member of the AF4 family of transcription factors involved in leukemia, is a positive transcription elongation factor-b-associated protein"J.Biomed.Sci.. 9. 234-245 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Mori, K., Kato, H.: "A putative nuclear receptor coactivator (TMF/ARA160) associates with hbrm/hSNF2a and BRG-1/hSNF2b and localizes in the Golgi apparatus."FEBS lett.. 520. 127-132 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Estable, M.C., Naghavi, M.H., Kato, H., Xiao, H., Qin, J., Vahlne, A., Roeder, R.G.: "MCEF, the newest member of the AF4 family of transcription factors involved in leukemia, is a positive transcription elongation factor-b-associated protein."J.Biomed.Sci.. 9. 234-245 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Tachibana, N., Sugimoto, K., Nozaki, N., Ueda, J., Ohta, T., Ohki, M., Fukuda, M., Takeda, N., Niida, H., Kato, H., Shinkai, Y.: "G9a histone methyltransferase plays a dominant role in euchromatic histone H3 lysine 9 methylation and is essential for early embryogenesis."Genes & Dev.. 16. 1779-1791 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] K.Mori: "A putative nuclear receptor coactivator(TMF/ARA160)associates with hbrm/hSNF2α and BRG-1/hSNF2β and localizes in the Golgi apparatus"FEBS lett.. 520. 127-132 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] M.Tachibana: "G9a histone methyltransferase plays a dominant role in euchromatic histone H3 lysine 9 methylation and is essential for early embryogenesis"Genes & Dev.. 16. 1779-1791 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] M.C.Estable: "MCEF, the newest member of the AF4 family of transcription factors involved in leukemia, is a positive transcription elongation factor-b-associated protein"J.Biomed.Sci.. 9. 234-245 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] K.Mori: "A putative nuclear receptor coactivator (TMF/ARA160) associates with hbrm/hSNF2α and BRG-1/hSNF2β and localizes in the Golgi apparatus"FEBS lett.. 520. 127-132 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] M.Tachibana: "G9a histone methyltransferase plays a dominant role in euchromatic histone H3 lysine 9 methylation and is essential for early embryogenesis"Genes & Dev.. 16. 1779-1791 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] M.C.Estable: "MCEF, the newest member of the AF4 family of transcription factors involved in leukemia, is a positive transcription elongation factor-b-associated protein"J. Biomed. Sci.. 9. 234-245 (2002)

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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