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Effect of Protein Conformation Fluctuation on the Election Transfer Rate through Protein Media

Research Project

Project/Area Number 14580670
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Biophysics
Research InstitutionMeijo University (2004)
Nagoya University (2002-2003)

Principal Investigator

KAKITANI Toshiaki  Meijo University, Science and Technology, Professor, 理工学部, 教授 (90027350)

Project Period (FY) 2002 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2004: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2003: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2002: ¥1,500,000 (Direct Cost: ¥1,500,000)
KeywordsElectron Transfer / Protein / Fluctuation / Energy gap dependence / Non-Condon effect / Inelastic electron tunneling / Time correlation function / Alternate route / 構造ゆらぎ / non-Condon effect / energy gap law / 非弾性トンネリング / 反応中心 / 電子移動速度制御 / トンネル効果 / タンパク質の構浩揺らぎ / 光合成細菌 / キノン / バクテリオクロロフィル / 電子移動速度ゆらぎ / タンパク質の構造揺らぎ
Research Abstract

Electron transfer (ET) through protein media is, in general, a long-range ET Namely, ETs take place from donor to acceptor by the tunneling mechanism, by feeling electric atmosphere from protein environment. So far, it was considered that there will be a region through which an electron is likely to be transferred. Usually chemical bonds and so on are considered as its candidate. If it happens, the election tunneling matrix element will not be changed considerably by the thermal fluctuation of protein conformation, because chemical bonds in the protein are not changed by the conformation fluctuation of protein. However, according to the results of our study in which protein conformation fluctuations are realized by the molecular dynamics (MID) simulation and electron tunneling matrix elements are calculated by the quantum chemical theory, electron tunneling matrix element is changed rapidly and greatly by the conformation fluctuation of proteins. As its mechanism, it is considered that … More interference among phases of tunneling currents works significantly. Indeed, it is a very interesting problem how the rapid fluctuation of the value in the electron tunneling matrix element affects the ET rate. We constructed a new non-Condon theory in which we can use the time correlation function of the electron tunneling matrix element obtained by the classical MD simulation. In this theory, the quantum, correction to the nuclear motion is performed and so, the detailed balance condition for the forward and backward rates is satisfied. As a result of analyzing the electron transfer in protein media using this new theory, we found that the energy gap dependence of the ET rate is greatly affected by the rapid fluctuation of the electron tunneling matrix element. Namely; we found that an inelastic tunneling mechanism works at the far normal region and far inverted region and then, the ET rates are much enhanced in both regions. We surveyed the experimental data which support this prediction. Then, we found that the ET rate does not decay appreciably at the very large energy gap in the Rehm-Weller experiment for the fluorescence quenching in polar solution, which is consistent with our theory: Less

Report

(4 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • 2002 Annual Research Report
  • Research Products

    (29 results)

All 2005 2004 2003 2002 Other

All Journal Article (21 results) Publications (8 results)

  • [Journal Article] Interference, fluctuation, and alternation of electron tunneling in protein media 1. Two tunneling routes in photosynthetic reaction center alternate due to thermal fluctuation of protein conformation2005

    • Author(s)
      T.Nishioka et al.
    • Journal Title

      J.Phys.Chem.B 109

      Pages: 1978-1987

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Prediction of inelastic electron tunneling mechanism from bacteriopheophytin to the primary quinone in modified bacterial photosynthetic reaction center2005

    • Author(s)
      H.Nishioka et al.
    • Journal Title

      Proceedings of the 13 th International Congress on Photosyn thesis 1

      Pages: 327-328

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Interference, fluctuation and alternation of electron tunneling in protein media 1. Two tunneling routes in photosynthetic reaction center alternate due to thermal fluctuation of protein conformation2005

    • Author(s)
      H.Nishioka et al.
    • Journal Title

      J.Phys.Chem.B 109

      Pages: 1978-1987

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Prediction of inelastic electron tunneling mechanism from bacteriopheophytin to the primary quinone in modified photosynthetic reaction center2005

    • Author(s)
      H.Nishioka et al.
    • Journal Title

      Proceedings of the 13 th International Congress of Photosynthesis 1

      Pages: 327-328

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Interference, fluctuation, and alternation of electron tunneling in protein media. 1. Two tunneling routes in photosynthetic reaction2005

    • Author(s)
      H.Nishioka, A.Kimura, T.Yamato, T.Kawatsu, T.Kakitani
    • Journal Title

      J.Phys.Chem.B 109

      Pages: 1978-1987

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Torsion potential works in rhodopsin2004

    • Author(s)
      A.Yamada et al.
    • Journal Title

      Photochem.Photobiol. 79

      Pages: 476-486

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Trsion potential works in rhodopsin2004

    • Author(s)
      A.Yamada et al.
    • Journal Title

      Photochem.Photobiol. 79

      Pages: 476-486

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Torsion potential works in rhodopsin2004

    • Author(s)
      A.Yamada, T.Yamato, T.Kakitani, S.Yamamoto
    • Journal Title

      Photochem.Photobiol. 79, 5

      Pages: 476-486

    • NAID

      110001158378

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Theoretical analysis of the energy gap dependence of the reconstituted B800→B850 excitation energy transfer rate in bacterial LH2 complexes2003

    • Author(s)
      A.Kimura et al.
    • Journal Title

      V.Phys.Chem.B 107

      Pages: 7932-7939

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Theory of excitation energy transfer in the intermediate coupling case of clusters2003

    • Author(s)
      A.Kimura et al.
    • Journal Title

      V.Phys.Chem.B 107

      Pages: 14486-14499

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Theoretical analysis of the energy gap dependence of the reconstituted B800→B850 excitation energy transfer rate in bacterial LH2 complexes2003

    • Author(s)
      A.Kimura et al.
    • Journal Title

      J.Phys.Chem.B 107

      Pages: 7932-7936

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Theory of excitation energy transfer in the intermediate coupling case of clusters2003

    • Author(s)
      A.Kimura et al.
    • Journal Title

      J.Phys.Chem.B 107

      Pages: 14486-14499

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Destructive interference in the electron tunneling through protein media2002

    • Author(s)
      T.Kawatsu et al.
    • Journal Title

      J.Phys.Chem.B 106

      Pages: 11356-11366

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] On the anomaly of the tunneling matrix element in long-range electron transfer2002

    • Author(s)
      T.Kawatsu et al.
    • Journal Title

      J.Phys.Chem.B 106

      Pages: 5068-5074

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Unique mechanisms of excitation energy transfer, electron transfer and photoisomerization in biological systems2002

    • Author(s)
      F.Kakitani et al.
    • Journal Title

      J.Biol.Phys. 28

      Pages: 367-381

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Empirical formula of exciton coherent domain in oligomers and application to LH22002

    • Author(s)
      T.Kakitani et al.
    • Journal Title

      J.Phys.Chem.B 106

      Pages: 2173-2179

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] A computational study on the stability of the protonated Schiff base of retinal in rhodopsin2002

    • Author(s)
      A.Yamada et al.
    • Journal Title

      Chem.Phys.Letters 366

      Pages: 670-675

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Analysis of cis-trans photoisomerization mechanism of rhodopsin based on the tertiary structure of rhodopsin2002

    • Author(s)
      A.Yamada et al.
    • Journal Title

      J.Photoscience 9

      Pages: 51-54

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] On the anomaly of the tunneling matrix element in the long-range electron transfer2002

    • Author(s)
      T.Kawatsu et al.
    • Journal Title

      J.Phys.Chem.B 106

      Pages: 5068-5074

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Unique mechanisms of excitation energy transfer, electron transfer and photoisomerization in biological systems2002

    • Author(s)
      T.Kakitani et al.
    • Journal Title

      J.Biol.Phys. 28

      Pages: 367-381

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Analysis of cis-trans photoisomerization mechanism based on the tertiary structure of rhodopsin2002

    • Author(s)
      A.Yamada et al.
    • Journal Title

      J.Photoscience 9

      Pages: 51-54

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Publications] A.Kimura, T.Kakitani: "Theoretical analysis of the energy gap dependence of the reconsisted B800→B850 excitation energy transfer rate in bacterial LH2 conplexes"J.Phys.Chem.B. 107, 31. 7932-7939 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] A.Kimura, T.Kakitani: "Theory of excitation energy transfer in the intermediate coupling case of clusters"J.Phys.Chem.B. 107, 51. 14486-14499 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] T.Kakitani et al.: "Empirical Formula of Excition Choherent Domain in Oligomers and Application to LH2"J.Phys.Chem.A.. 106,10. 2173-2179 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] T.Kawatsu et al.: "On the Anomaly of the Tunneling Matrix Element in Long-Range Electron Transfer"J.Phys.Chem.B. 106,19. 5068-5074 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] T.Kakitani, et al.: "Unique Mechanisms of Excitation Energy Transfer, Electron Transfer and Photoisomerization in Biological Systems"V. Biol. Phys.. 28. 367-381 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] A.Yamada et al.: "Analysis of Cis-Trans Photoisomerization Mechanism of Rhodopsin Based on the Tertiary Structure of Rhodopsin"J.Photoscience. 9,2. 51-54 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] T.Kawatsu et al.: "Destructive Interference in the Electron Tunneling through Protein Media"J. Phys. Chem. B. 106,43. 11356-11366 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] A.Yamada et al.: "A Computational Study on the Stability of the Photonated Schiff Base of Retinal in Rhodopsin"Chem. Phys. Letters. 366. 670-675 (2002)

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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