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Development of photo-driven motor proteins using phtochromic molecules

Research Project

Project/Area Number 14580677
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Biophysics
Research InstitutionSoka University

Principal Investigator

MARUTA Shinsaku  Soka University, Dept.of Bioinformatics, Professor, 工学部, 教授 (40231732)

Co-Investigator(Kenkyū-buntansha) 茶圓 茂  日本大学, 理工学部, 教授 (60142452)
Project Period (FY) 2002 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2005: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2004: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2003: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2002: ¥1,100,000 (Direct Cost: ¥1,100,000)
Keywordsmolecular motor / myosin / kinesin / photochromic molecule / azobenzene / laser / energy transduction / ATPase / 化学修飾 / X線溶液散乱 / 蛍光 / 構造変化
Research Abstract

ATP driven-motor proteins transduce chemical energy generated from ATP hydrolysis into the physical motion energy with the mechanical system like cam-shaft. It is expected that introduction of artificial energy input device into the energy transducing site of motor protein, enable the driving or regulating of motor proteins without ATP. In the present study, photochromic molecules which change their conformation reversibly with high response due to the isomerization by ultraviolet (UV) and visible light (VIS) irradiation, have been utilized as energy input device. Especially azobenzene and spiropyran derivatives, which change their characteristics dramatically on isomerization by UV-VIS light irradiation, might work effectively.
In this study, several kinds of azobenzene and spiropyran derivatives, which can bind to cysteine side chain covalently, have been synthesized. And several motor protein mutants, which have one reactive cysteine at the specific site, have been also prepared. It has been demonstrated that for skeletal muscle myosin motor domain, the localized conformational changes resulting from isomerization on azobenzene derivative cross-linking of reactive cysteine residues, SH1 and SH2 induced swing in the lever arm portion. It was shown that the incorporated photochromic group acts as a mechanical energy input in a motor protein. The new technique using photochromic molecules may be applicable to other energy transducing and regulatory proteins.

Report

(5 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • 2003 Annual Research Report
  • 2002 Annual Research Report
  • Research Products

    (23 results)

All 2006 2004 Other

All Journal Article (18 results) Publications (5 results)

  • [Journal Article] Preparation and characterization of a novel rice plant specific kinesin2006

    • Author(s)
      Umeki, N., Mitsui, T., Umezu, N., kondo, K., Maruta, S.
    • Journal Title

      Journal of Biochemistry 139

      Pages: 645-654

    • NAID

      10018845372

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Intermolecular cross-linking of a novel rice kinesin K16 motor domain with a photoreactive ATP derivative2006

    • Author(s)
      Umeki, N., Mitsui, T., Koike, Y., Maruta, S.
    • Journal Title

      Journal of Biochemistry 139

      Pages: 831-836

    • NAID

      10018844848

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Conformational change of the loop L5 in rice kinesin motor domain induced by nucleotide binding2006

    • Author(s)
      Umeki, N., Mitsui, T., Kondo, K., Maruta, S.
    • Journal Title

      Journal of Biochemistry 139

      Pages: 857-864

    • NAID

      10018844929

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Preparation and characterization of a novel rice plant specific kinesin2006

    • Author(s)
      Umeki, N., Mitsui, T., Umezu, N., Kondo, K., Maruta, S
    • Journal Title

      J.Biochem. 139

      Pages: 645-654

    • NAID

      10018845372

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Intermolecular cross-linking of a novel rice kinesin K16 motor domain with a photoreactive ATP derivative2006

    • Author(s)
      Umeki, N., Mitsui, T., Koike, Y., Maruta, S.
    • Journal Title

      J.Biochem. 139

      Pages: 831-836

    • NAID

      10018844848

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Conformational change of the loop L5 in rice kinesin motor domain induced by nucleotide binding2006

    • Author(s)
      Umeki, N., Mitsui, T., Kondo, K., Maruta, S.
    • Journal Title

      J.Biochem. 139

      Pages: 857-864

    • NAID

      10018844929

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Preparation and characterization of a novel rice plant specific kinesin2006

    • Author(s)
      Umeki, N., Mitsui, T., Umezu, N., Kondo, K., Maruta, S.
    • Journal Title

      Journal of Biochemistry (In press)

    • NAID

      10018845372

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Intermolecular cross-linking of a novel rice kinesin K16 motor domain with a photoreactive ATP derivative2006

    • Author(s)
      Umeki, N., Mitsui, T., Koike, Y., Maruta, S.
    • Journal Title

      Journal of Biochemistry (In press)

    • NAID

      10018844848

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Conformational change of the loop L5 in rice kinesin motor domain induced by nucleotide binding2006

    • Author(s)
      Umeki, N., Mitsui, T., Kondo, K., Maruta, S.
    • Journal Title

      Journal of Biochemistry (In press)

    • NAID

      10018844929

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Three-dimensional structural analysis of individual myosin heads undervarious functional states2004

    • Author(s)
      Katayama, E., Ichise, N., Yaeguchi, N., Yoshizawa, T., Maruta, S., Baba, N.
    • Journal Title

      Adv. In Exp. Med. And Biol. 538

      Pages: 295-304

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Interaction of myosin-ADP-fluorometal complexes with fluorescent probes and direct observation using quick-freeze deep-etch electron microscopy2004

    • Author(s)
      Maruta, S., Uyehara, Y., Aihara, T., Katayama, E.
    • Journal Title

      Journal of Biochemistry 136

      Pages: 57-64

    • NAID

      10016200785

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Incorporation of an azobenzene derivative into the energy transducing site of skeletal muscle myosin results in photo-induced conformational changes2004

    • Author(s)
      Umeki, N., Yoshizawa, T., Sugimoto, Y., Mitsui, T., Wakabayashi, K., Maruta, S.
    • Journal Title

      Journal of Biochemistry 136

      Pages: 839-846

    • NAID

      10016204499

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Three-dimensional structural analysis of individual myosin heads undervarious functional states2004

    • Author(s)
      Katayama, E., Ichise, N., Yaeguchi, N., Yoshizawa, T., Maruta, S., Baba, N.
    • Journal Title

      Adv.In Exp.Med.And Biol. 538

      Pages: 295-304

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Interaction of myosin ・ADP・ fluorometal complexes with fluorescent probes and direct obervation using quick-freeze deep-etch electron microscopy2004

    • Author(s)
      Maruta, S., Uyehara, Y., Aihara, T., Katayama, E.
    • Journal Title

      J.Biochem. 136

      Pages: 57-64

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Incorporation of an azobenzene derivative into the energy transducing site of skeletal muscle myosin results in photo-induced conformational changes2004

    • Author(s)
      Umeki, N., Yoshizawa, T., Sugimoto, Y., Mitsui, T., Wakabayashi, K., Maruta, S.
    • Journal Title

      J.Biochem. 136

      Pages: 839-846

    • NAID

      10016204499

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Three-dimensional structural analysis of individual myosin heads undervarious functional states.2004

    • Author(s)
      Katayama, E., Ichise, N., Yaeguchi, N., Yoshizawa, T., _Maruta, S., Baba, N.
    • Journal Title

      Adv.In Exp.Med. And Biol. 588

      Pages: 295-304

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Interaction of myosin・ADP・fluorometal complexes with fluorescent probes and direct observation using quick-freeze deep-etch electron microscopy2004

    • Author(s)
      Maruta, S., Uyehara, Y., Aihara, T., Katayama, E.
    • Journal Title

      Journal of Biochemistry 136

      Pages: 57-64

    • NAID

      10016200785

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Incorporation of an azobenzene derivative into the energy transducing site of skeletal muscle myosin results in phto-induced conformational change2004

    • Author(s)
      Umeki, N., Yoshizawa, T., Sugimoto, Y., Mitsui, T., Wakabayashi, K., Maruta
    • Journal Title

      Journal of Biochemistry 136

      Pages: 839-846

    • Related Report
      2004 Annual Research Report
  • [Publications] Komaba, S., Inoue, A., Maruta, S., Hosoya, H., Ikebe, _M.: "Determination of Human Myosin III as a Motor Protein Having a Protein Kinase Activity"J.Biol.Chem.. 278. 21352-21360 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] E.Katayama, N.Ichise, N.Yaeguchi, T.Yoshizawa, S.Maruta, N.Baba: "Three-dimensional structural analysis of individual myosin heads undervarious functional states"Adv.in Exp.Med. and Biol.. 538. 295-304 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Maruta, S., Mizukura, Y., Chaen, S.: "Interaction of a New Fluorescent ATP Analogue with Skeletal Muscle Myosin Subfragment-1"J. Biochem.. 131. 905-911 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Maruta, S., Mizukura, Y.: "Analysis of the Conformational Change of Myosin during ATP Hydrolysis Using Fluorescence Resonance Energy Transfer"J. Biochem.. 132. 471-482 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Shibuya, H., Kondo, K., Kimura, N., Maruta, S.: "Formation and Characterization of Kinesin・ADP・Fluorometal Complexes"J. Biochem.. 132. 573-579 (2002)

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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