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Translational and rotational positioning of nucleosomes and transcriptional regulation

Research Project

Project/Area Number 14580679
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Molecular biology
Research InstitutionTohoku University

Principal Investigator

KOMURA Jun-ichiro  Tohoku University, Graduate School of Medicine, Research Associate, 大学院・医学系研究科, 助手 (10215410)

Project Period (FY) 2002 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 2003: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 2002: ¥1,700,000 (Direct Cost: ¥1,700,000)
Keywordschromatin / nucleosome / ionizing radiation / 電解放射線
Research Abstract

1.I have developed a new method for determining, with resolution at the nucleotide level, the rotational positions of nucleosomes along single-copy genes in mammalian cells, by combining X-irradiation with ligation-mediated PCR.
2.I have analyzed the influence of the transcriptional activation of the c-FOS gene on a nucleosome positioned in the promoter of this gene. When human fibroblasts were serum-induced, no change in the position of this nucleosome was detected, although concurrent chemical modifications of nucleosomes in the c-FOS region upon induction have been reported.
3.I have studied cell cycle-dependent changes in the positioning of nucleosomes in the promoters of the c-FOS gene and the RNU6 gene. In mitotic HeLa cells, the nucleosome positioning in these promoters appeared to be lost. This result suggests random distribution of nucleosomes in mitotic chromososmes.

Report

(3 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • Research Products

    (3 results)

All Other

All Publications (3 results)

  • [Publications] Jun-ichiro Komura: "Nucleosome positioning in the human c-FOS promoter analyzed by in vivo footprinting with psoralen and ionizing radiation."Biochemistry. 42. 15084-15091 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Jun-ichiro Komura, Tetsuya Ono: "Nucleosome positioning in the human c-Fos promoter analyzed by in vivo footprinting with psoralen and ionizing radiation"Biochemistry. 42(51). 15084-15091 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Jun-ichiro Komura: "Nucleosome positioning in the human c-FOS promoter analyzed by in vivo footprinting with psoralen and ionizing radiation"Biochemistry. 42(51). 15084-15091 (2003)

    • Related Report
      2003 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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