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THE ROLE OF THE TRANSLATIONAL REGULATOR NAT1 IN DEVELOPMENT

Research Project

Project/Area Number 14580714
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Developmental biology
Research InstitutionNARA INSTITUTE OF SCIENCE AND TECHNOLOGY

Principal Investigator

YAMANAKA Shinya  NARA INSTITUTE OF SCIENCE AND TECHNOLOGY, CENTER FOR GENETIC INFORMATION, PROFESSOR, 遺伝子教育研究センター, 教授 (10295694)

Co-Investigator(Kenkyū-buntansha) NAKAMURA Makoto  NATIONAL INSTITUTE FOR BASIC BIOLOGY, DIVISION FOR MORPHOGENESIS, RESEARCH ASSOCIATE, 基礎生物学研究所, 助手 (30212103)
Project Period (FY) 2002 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2003: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 2002: ¥1,900,000 (Direct Cost: ¥1,900,000)
KeywordsTranslational control / non-AUG / ES cell / Paralogue / 翻訳調節 / ES細胞 / プロテオミックス / 発癌 / 細胞周期
Research Abstract

Only a few cases of exclusive translation initiation at non-AUG codons have been reported. We recently demonstrated that mammalian NAT1 mRNA, encoded by E1F4G2, uses GUG as its only translation initiation codon. In this study, we identified NAT1 orthologs from chicken, Xenopus, and zebrafish and found that in all species, the GUG codons also serve as the initiation codon. In all species, the GUG codons fulfilled the reported requirements for non-AUG initiation : an optimal Kozak motif and a downstream hairpin structure. Site-directed mutagenesis showed that nucleotides at positions -3 and +4 are critical for the GUG-mediated translation initiation in vitro. We found that NAT1 orthologs in Drosophila melanogaster and Halocynthia roreizi also use non-AUG start codons, demonstrating evolutional conservation of the non-canonical translation initiation.

Report

(3 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • Research Products

    (6 results)

All 2005 2004 Other

All Journal Article (3 results) Publications (3 results)

  • [Journal Article] Evolutionarily Conserved Non-AUG Translation Initiation in NAT1/p97/DAP5(EIF4G2)2005

    • Author(s)
      Takahashi K
    • Journal Title

      Genomics 85

      Pages: 360-371

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Evolutionarily Conserved Non-AUG Translation Initiation in NAT1/p97/DAP5 (E1F4G2)2005

    • Author(s)
      Takahashi K
    • Journal Title

      Genomics 85

      Pages: 360-371

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] 哺乳類の初期発生とES細胞分化におけるe1F4G関連タンパク質NAT1の役割2004

    • Author(s)
      笹岡 由美子
    • Journal Title

      実験医学 22

      Pages: 213-217

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Mitsui K: "The homeoprotein Nanog is required for maintenance of pluripotency in mouse epiblast and ES cells"Cell. 113. 631-642 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Takahashi K: "Role of ERas in promoting tumor-like properties in mouse embryonic stem cells."Nature. 423. 541-545 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Y.Tokuzawa: "Fbx15 is a novel target of Oct3/4 but is dispensable for ES cell self-renewal and mouse development"Molecular and Cellular Biology. (in press).

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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