| Project/Area Number |
14580718
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Developmental biology
|
|---|
| Research Institution | Okazaki National Research Institute |
|---|
Principal Investigator |
NAKAMURA Makoto Okazaki National Research Institute, National Institute for Basic Biology, Department of Developmental Biology, Research Associate, 基礎生物学研究所, 助手 (30212103)
|
|---|
| Project Period (FY) |
2002 – 2003
|
| Project Status |
Completed (Fiscal Year 2003)
|
| Budget Amount *help |
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2003: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2002: ¥2,500,000 (Direct Cost: ¥2,500,000)
|
|---|
| Keywords | TONAS / PML nuclear body / SUMO / Drosophila / transcription / epigenetics / tonalli / 癌関連遺伝子 |
|---|
| Research Abstract |
We isolated mutant alleles of tonalli (tna), one of the trithorax group genes, by genetic screening for dominant suppressors of the DPP signal-dependent wing-outgrowth phenotype in Drosophila. We also isolated vertebrate homologues of tna, TUNAS-1 and TUNAS-2. The most characteristic feature of these proteins is the existence of a single SP-RING finger motif in the middle. The SP-RING motif was originally found in the PIAS family SUMO-E3 ligase proteins. In this study, we found that TUNAS has SUMO-E3 ligase activity and TUNAS facilitates specific SUMO-2/3 conjugation to TUNAS itself. TUNAS also shows strong activity of nuclear body formation in cultured cells. TUNAS effectively recruits transcriptional regulators including PML, CBP and P300 to the nuclear body. Our results are suggesting a role for TNA and TUNAS in the connection of the Trithorax/SWI/SNF chromatin remodeling complex to CBP/P300, the relocation of these protein complexes into the nuclear substructure, and the regulation of gene expression.
|
