A possible role for an anti-apoptotic protein, survivin, in survival of neurons in the brain
| Project/Area Number |
14580750
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Neurochemistry/Neuropharmacology
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| Research Institution | Nippon Medical School |
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Principal Investigator |
OHSAWA Ikuroh Nippon Medical School, Institute of Gerontology, Lecturer, 老人病研究所, 講師 (30343586)
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| Co-Investigator(Kenkyū-buntansha) |
OHTA Shigeo Nippon Medical School, Professor, 大学院・医学研究科, 教授 (00125832)
ASOH Sadamitsu Nippon Medical School, Associated professor, 医学部, 助教授 (70167914)
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| Project Period (FY) |
2002 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2004: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2003: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2002: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Keywords | central nervous system / neuronal survival / survivin / apoptosis / immunostaining / glioma / prognosis / ELISA / トランスジェニック・マウス |
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| Research Abstract |
Precise mechanism for survival of neurons in central nervous system remains to be elucidated. It includes many anti-apoptotic proteins. Survivin is a member of the inhibitor-of-apoptosis protein. Its expression is determined in the developing embryo and in rapidly dividing cells including many human cancers. Previous studies demonstrated that survivin is expressed at G2/M in a cell-cycle dependent manner and associated with kinetochores of metaphase chromosomes and the central spindle midzone at anaphase, suggesting that survivin participates as a chromosomal passenger protein in cleavage furrow formation. Forced expression of survivin counteracted cell death induced by various apoptotic stimuli, whereas interference with the expression or function of survivin by a dominant negative form or antisense of survivin caused spontaneous apoptosis and multiple cell division defects, suggesting that survivin acts both as a mitotic regulator and as a cytoprotective factor at cell division. Then
… More
, we prepared polyclonal anti-survivin serum to investigate the expression of survivin in central nervous system. At first, we stained primary cultured cells derived from neocortex in rat embryo and found that survivin was expressed in MAP2 positive differentiated neurons. The expression was also observed in a part of astrocytes. Immunological staining of mouse brain section confirmed the expression of survivin in neurons. These results suggested that survivin might be included in anti-apoptotic machineries of differentiated neurons. On the other hand, we examined whether survivin expression in human gliomas would be a correlative of prognosis, and found that higher expression of survivin was correlated with the survival of gliomas. Thus, immunostaining of glioma with anti-survivin serum would be a powerful tool for a clinical prognosis. We also investigated whether urine surviving is useful for diagnosing bladder cancer, and found that ELISA system for survivin might be a useful tumor marker. Less
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Report
(4 results)
Research Products
(40 results)
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[Journal Article] Prognostic significance of the immunohistochemical index of surviving in glioma : a comparative study with the MIB-1 index.2005
Author(s)
Uematsu, M., Ohsawa, I., Aokage, T., Nishimaki, K., Matsumoto, K., Takahashi, H., Asho, S., Teramoto, A., Ohta, S.
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Journal Title
J.Neuro-Oncol. (in press)
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Journal Article] The anti-cell death FNK protein protects cells from death induced by freezing and thawing.2005
Author(s)
Sudo, K., Asoh, S., Ohsawa, I., Ozaki, D., Yamagata, K., Ito, H., Ohta S.
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Journal Title
Biochem.Biophys.Res.Commun. 330
Pages: 850-856
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Genetic deficiency of a mitochondrial aldehyde dehydrogenase increases serum lipid peroxides in community-dwelling females.2003
Author(s)
Ohsawa, I., Kamino, K., Nagasaka, K., Ando, F., Niino, N., Shimokata, H., Ohta, S.
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Journal Title
J.Hum.Genet. 48
Pages: 404-409
NAID
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Continuous culture of novel mitochondrial cells lacking nuclei.2003
Author(s)
Nakano, K., Ohsawa, I., Yamagata, K., Nakayama.T, Sasaki, K., Tarashima, M., Saito, K., Osawa, M., Ohta, S.
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Journal Title
Mitochondrion 3
Pages: 21-27
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Protection against ischemic brain injury by protein therapeutics. ^*Equal contributor2002
Author(s)
Asoh, S.^*, Ohsawa, I.^*, Mori, T., Katsura, K., Hiraide, T., Katayama, Y, Kimura, M., Ozaki, D., Yamagata, K., Ohta,.S.
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Journal Title
Proc.Nat.Acad.Sci.USA. 99
Pages: 17107-17112
Description
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[Journal Article] Down-regulation of a novel gene, DRLM, in human liver malignancy from 4q22 that encodes a NAP-like protein.2002
Author(s)
Harada, H., Nagai, H., Ezura, Y., Yokota, T., Ohsawa, I., Yamaguchi, K., Ohue, C., Tsuneizumi, M., Mikami, I., Terada, Y., Yabe, A., Emi, M.
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Description
「研究成果報告書概要(欧文)」より
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[Book] 酸化ストレスナビゲーター2005
Author(s)
倉林正彦
Total Pages
331
Publisher
メディカルビュー社
Description
「研究成果報告書概要(和文)」より
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