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A possible role for an anti-apoptotic protein, survivin, in survival of neurons in the brain

Research Project

Project/Area Number 14580750
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Neurochemistry/Neuropharmacology
Research InstitutionNippon Medical School

Principal Investigator

OHSAWA Ikuroh  Nippon Medical School, Institute of Gerontology, Lecturer, 老人病研究所, 講師 (30343586)

Co-Investigator(Kenkyū-buntansha) OHTA Shigeo  Nippon Medical School, Professor, 大学院・医学研究科, 教授 (00125832)
ASOH Sadamitsu  Nippon Medical School, Associated professor, 医学部, 助教授 (70167914)
Project Period (FY) 2002 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2004: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2003: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2002: ¥1,300,000 (Direct Cost: ¥1,300,000)
Keywordscentral nervous system / neuronal survival / survivin / apoptosis / immunostaining / glioma / prognosis / ELISA / トランスジェニック・マウス
Research Abstract

Precise mechanism for survival of neurons in central nervous system remains to be elucidated. It includes many anti-apoptotic proteins. Survivin is a member of the inhibitor-of-apoptosis protein. Its expression is determined in the developing embryo and in rapidly dividing cells including many human cancers. Previous studies demonstrated that survivin is expressed at G2/M in a cell-cycle dependent manner and associated with kinetochores of metaphase chromosomes and the central spindle midzone at anaphase, suggesting that survivin participates as a chromosomal passenger protein in cleavage furrow formation. Forced expression of survivin counteracted cell death induced by various apoptotic stimuli, whereas interference with the expression or function of survivin by a dominant negative form or antisense of survivin caused spontaneous apoptosis and multiple cell division defects, suggesting that survivin acts both as a mitotic regulator and as a cytoprotective factor at cell division. Then … More , we prepared polyclonal anti-survivin serum to investigate the expression of survivin in central nervous system. At first, we stained primary cultured cells derived from neocortex in rat embryo and found that survivin was expressed in MAP2 positive differentiated neurons. The expression was also observed in a part of astrocytes. Immunological staining of mouse brain section confirmed the expression of survivin in neurons. These results suggested that survivin might be included in anti-apoptotic machineries of differentiated neurons. On the other hand, we examined whether survivin expression in human gliomas would be a correlative of prognosis, and found that higher expression of survivin was correlated with the survival of gliomas. Thus, immunostaining of glioma with anti-survivin serum would be a powerful tool for a clinical prognosis. We also investigated whether urine surviving is useful for diagnosing bladder cancer, and found that ELISA system for survivin might be a useful tumor marker. Less

Report

(4 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • 2002 Annual Research Report
  • Research Products

    (40 results)

All 2005 2004 2003 2002 Other

All Journal Article (28 results) Book (1 results) Patent(Industrial Property Rights) (3 results) Publications (8 results)

  • [Journal Article] The anti-cell death FNK protein protects cells from death induced by freezing and thawing.2005

    • Author(s)
      Sudo, K.
    • Journal Title

      Biochem.Biophys.Res.Commun. 330

      Pages: 850-856

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Prognostic significance of the immunohistochemical index of surviving in glioma : a comparative study with the MIB-1 index2005

    • Author(s)
      Uematsu, M.
    • Journal Title

      J.Neuro-Oncol. (In press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Prognostic significance of the immunohistochemical index of surviving in glioma : a comparative study with the MIB-1 index.2005

    • Author(s)
      Uematsu, M., Ohsawa, I., Aokage, T., Nishimaki, K., Matsumoto, K., Takahashi, H., Asho, S., Teramoto, A., Ohta, S.
    • Journal Title

      J.Neuro-Oncol. (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] The anti-cell death FNK protein protects cells from death induced by freezing and thawing.2005

    • Author(s)
      Sudo, K., Asoh, S., Ohsawa, I., Ozaki, D., Yamagata, K., Ito, H., Ohta S.
    • Journal Title

      Biochem.Biophys.Res.Commun. 330

      Pages: 850-856

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Prognostic significance of the immunohistchemical index of survivin in glioma: A comparative study with the MIB-1 index2005

    • Author(s)
      Uematsu, M.
    • Journal Title

      J.Neurooncol. (In press)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Green fluorescent protein causes mitochondria to aggregate in the presence of the Bc1-2 family proteins2004

    • Author(s)
      Aokage, T.
    • Journal Title

      Biochem.Biophy.Res.Commun. 314

      Pages: 711-716

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Mitochondrial ALDH2 deficiency as an oxidative stress2004

    • Author(s)
      Ohta, S.
    • Journal Title

      Ann.N.Y.Acad.Sci. 1011

      Pages: 36-44

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Detection of urine survivin in 40 patients with bladder cancer.2004

    • Author(s)
      Ohsawa, I.
    • Journal Title

      J.Nippon Med.Sch. 71

      Pages: 379-383

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Alcohol dehydrogenase 2 variant is associated with cerebral infarction and lacunae.2004

    • Author(s)
      Suzuki, Y.
    • Journal Title

      Neurology 63

      Pages: 1711-1713

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Alcohol dehydrogenase 2 variant is associated with cerebral infarction and lacunae.2004

    • Author(s)
      Suzuki Y., Fujisawa M., Ando F., Niino N., Ohsawa I., Shimokata H., Ohta S.
    • Journal Title

      Neurology 63

      Pages: 1711-1713

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Detection of urine survivin in 40 patients with bladder cancer.2004

    • Author(s)
      Ohsawa, I., Nishimura, T., Kondo, Y., Kimura, G., Ohta, S.
    • Journal Title

      J.Nippon Med.Sch. 71

      Pages: 379-383

    • NAID

      130004146764

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Mitochondrial ALDH2 deficiency as an oxidative stress.2004

    • Author(s)
      Ohta, S., Ohsawa, I., Kamino, K., Ando, F., Shimokata, H.
    • Journal Title

      Ann.N.Y.Acad.Sci. 1011

      Pages: 36-44

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Green fluorescent protein causes mitochondria to aggregate in the presence of the Bcl-2 family proteins.2004

    • Author(s)
      Aokage, T., Ohsawa, I., Ohta, S.
    • Journal Title

      Biochem.Biophys.Res.Commun. 314

      Pages: 711-716

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Green fluorescent protein causes mitochondria to aggregate in the presence of the Bcl-2 family proteins2004

    • Author(s)
      Aokage, T.
    • Journal Title

      Biochem.Biophys.Res.Commun. 314・3

      Pages: 711-716

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Detection of urine survivin in 40 patients with bladder cancer2004

    • Author(s)
      Ohsawa, I.
    • Journal Title

      J.Nippon Med.Sch. 71・6

      Pages: 379-383

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Continuous culture of novel mitochondrial cells lacking nuclei2003

    • Author(s)
      Nakano, K.
    • Journal Title

      Mitochondrion 3

      Pages: 21-27

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Genetic deficiency of a mitochondrial aldehyde dehydrogenase increases serum lipid peroxides in community-dwelling females2003

    • Author(s)
      Ohsawa, I.
    • Journal Title

      J.Hum.Genet. 48

      Pages: 404-409

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Deficiency in a mitochondrial aldehyde dehydrogenase increases vulnerability to oxidative stress in PC12 cells.2003

    • Author(s)
      Ohsawa, I.
    • Journal Title

      J.Neurochem. 84

      Pages: 1110-1117

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Deficiency in a mitochondrial aldehyde dehydrogenase increases vulnerability to oxidative stress in PC12 cells.2003

    • Author(s)
      Ohsawa, I., Nishimaki, K., Yasuda, C., Kamino, K., Ohta, S.
    • Journal Title

      J.Neurochem. 84

      Pages: 1110-1117

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Genetic deficiency of a mitochondrial aldehyde dehydrogenase increases serum lipid peroxides in community-dwelling females.2003

    • Author(s)
      Ohsawa, I., Kamino, K., Nagasaka, K., Ando, F., Niino, N., Shimokata, H., Ohta, S.
    • Journal Title

      J.Hum.Genet. 48

      Pages: 404-409

    • NAID

      10011595420

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Continuous culture of novel mitochondrial cells lacking nuclei.2003

    • Author(s)
      Nakano, K., Ohsawa, I., Yamagata, K., Nakayama.T, Sasaki, K., Tarashima, M., Saito, K., Osawa, M., Ohta, S.
    • Journal Title

      Mitochondrion 3

      Pages: 21-27

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Deficiency in a mitochondrial aldehyde dehydrogenase increases vulnerability to oxidative stress in PC12 cells.2003

    • Author(s)
      Ohsawa, I.
    • Journal Title

      J.Neuchem. 84・5

      Pages: 1110-1117

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Genetic deficiency of a mitochondrial aldehyde dehydrogenase increases serum lipid peroxides in community-dwelling females2003

    • Author(s)
      Ohsawa, I.
    • Journal Title

      J.Hum.Genet. 48・8

      Pages: 404-409

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Protection against ischemic brain injury by protein therapeutics.2002

    • Author(s)
      Asoh, S.
    • Journal Title

      Proc.Nat.Acad.Sci.USA. 99

      Pages: 17107-17112

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Down-regulation of a novel gene, DRLM, in human liver malignancy from 4q22 that encodes a NAP-like protein.2002

    • Author(s)
      Harada, H.
    • Journal Title

      Gene 296

      Pages: 171-177

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Protection against ischemic brain injury by protein therapeutics. ^*Equal contributor2002

    • Author(s)
      Asoh, S.^*, Ohsawa, I.^*, Mori, T., Katsura, K., Hiraide, T., Katayama, Y, Kimura, M., Ozaki, D., Yamagata, K., Ohta,.S.
    • Journal Title

      Proc.Nat.Acad.Sci.USA. 99

      Pages: 17107-17112

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Down-regulation of a novel gene, DRLM, in human liver malignancy from 4q22 that encodes a NAP-like protein.2002

    • Author(s)
      Harada, H., Nagai, H., Ezura, Y., Yokota, T., Ohsawa, I., Yamaguchi, K., Ohue, C., Tsuneizumi, M., Mikami, I., Terada, Y., Yabe, A., Emi, M.
    • Journal Title

      Gene 296

      Pages: 171-177

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Protection against ischemic brain injury by protein therapeutics.2002

    • Author(s)
      Asoh, S.
    • Journal Title

      Proc.Nat.Acad.Sci.USA. 99・26

      Pages: 17107-17112

    • Related Report
      2004 Annual Research Report
  • [Book] 酸化ストレスナビゲーター2005

    • Author(s)
      倉林正彦
    • Total Pages
      331
    • Publisher
      メディカルビュー社
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Patent(Industrial Property Rights)] 酸化ストレスに伴う老化関連疾患を示すトランスジェニック動物2003

    • Inventor(s)
      太田 成男 他
    • Industrial Property Rights Holder
      学校法人日本医科大
    • Industrial Property Number
      2003-307490
    • Filing Date
      2003-08-29
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Patent(Industrial Property Rights)] 酸化ストレスに伴う老化関連疾患を示すトランスジェニック動物2003

    • Inventor(s)
      太田 茂男, 大澤 郁朗
    • Industrial Property Rights Holder
      学校法人日本医科大学
    • Industrial Property Number
      2003-307490
    • Filing Date
      2003-08-29
    • Related Report
      2004 Annual Research Report
  • [Patent(Industrial Property Rights)] 腫瘍診断剤およびその用途2002

    • Inventor(s)
      太田 成男 他
    • Industrial Property Rights Holder
      (株)免疫生物研究所
    • Industrial Property Number
      2002-303893
    • Filing Date
      2002-10-18
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Publications] Harada, H.: "Down-regulation of a novel gene, DRLM, in human liver malignancy from 4q22 that encodes a NAP-like protein."Gene. 296・1-2. 171-177 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] Asoh, S.: "Protection against ischemic brain injury by protein therapeutics."Proc.Nat.Acad.Sci.USA.. 99・26. 17107-17112 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] Ohsawa, I.: "Deficiency in a mitochondrial aldehyde dehydrogenase increases vulnerability to oxidative stress in PC12 cells."J.Neuchem.. 84・5. 1110-1117 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Ohsawa, I.: "Genetic deficiency of a mitochondrial aldehyde dehydrogenase increases serum lipid peroxides in community-dwelling females"J.Hum.Genet.. 48・8. 404-409 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Aokage, T.: "Green fluorescent protein causes mitochondria to aggregate in the presence of the Bcl-2 family proteins"Biochem.Biophy.Res.Commun.. 314・3. 711-716 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Harada, H.: "Down-regulation of a novel gene, DRLM, in human liver malignancy from 4q22 that encodes a NAP-like protein"Gene. 296. 171-177 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Asoh, S.: "Protection against ischemic brain injury by protein therapeutics"Proc. Nat. Acad. Sci. USA.. 99. 17107-17112 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Ohsawa. I.: "Deficiency in a mitochondrial aldehyde dehydrogenase increases vulnerability to oxidative stress in PC12 cells"J. Neuchem.. 84. 1110-1117 (2003)

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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