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Studies on cytokine-induced sleep in prostanoid-related gene-manipulated mice

Research Project

Project/Area Number 14580755
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Neurochemistry/Neuropharmacology
Research InstitutionOsaka Bioscience Institute

Principal Investigator

HAYAISHI Osamu  Osaka Biosci.Inst., dept.of Mol.Behav. Biol., Research Associate, 分子行動生物学部門, 研究員 (40025507)

Co-Investigator(Kenkyū-buntansha) SAKATA Mie  Osaka Biosci.Inst., dept.of Mol.Behav. Biol., Postdoctoral Associate, 分子行動生物学部門, 研究員 (10353525)
URADA Yoshihiro  Osaka Biosci.Inst., dept.of Mol.Behav. Biol., Head, 分子行動生物学部門, 研究部長 (10201360)
EGUCHI Naomi  Osaka Biosci.Inst., dept.of Mol.Behav. Biol., Vice Head, 分子行動生物学部門, 研究副部長 (10250086)
Project Period (FY) 2002 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥4,100,000 (Direct Cost: ¥4,100,000)
Fiscal Year 2003: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 2002: ¥2,500,000 (Direct Cost: ¥2,500,000)
Keywordsinflammation / sytokine / IL-1β / TNF-α / Sleep / Prostaglandin / knockout mouse
Research Abstract

Infectious illness is associated with an increase in the amount of sleep and causes the production of proinflammatory cytokines and induction of cyclooxygenase-2 (COX-2), which is the key enzyme of the biosynthesis of prostaglandins (PGs). Both IL-1β and PGD_2 promote sleep in rats and mice. Pretreatment of COX-2 inhibitor reportedly blocked the IL-1β-promoted sleep in rats. Therefore, PGs are considered to mediate the somnogenic effect of IL-1β in rats. However, little is known about the molecular interaction between PGs and cytokines in the sleep-wake regulation in mice. Here, we employed the PGD_2 receptor-knockout mice and highly selective COX-2 inhibitor NS-398 to examine the involvement of PGD2 in the somnogenic effect of IL-1β in mice. IL-1β increased total amount of non-rapid eye movement (NREM) sleep in both wild-type (WT) and PGD_2 receptor-knockout mice and concomitantly reduced body temperature and locomotor activity for 6h after the intraperitoneal injection at 8 p.m. Pretreatment ofNS-398 inhibited neither spontaneous nor IL-1β-induced sleep in WT mice. These results indicate that PGD_2 is not involved in the somnogenic effect of IL-1β in mice.

Report

(3 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • Research Products

    (50 results)

All Other

All Publications (50 results)

  • [Publications] Hayaishi, O.: "Molecular genetic studies on sleep-wake regulation, with special emphasis on the prostaglandin D_2 system."J.Appl.Physiol.. 92(2). 863-868 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hayaishi, O., Urade, Y.: "Prostaglandin D_2 in sleep-wake regulation : recent progress and perspectives."Neuroscientist. 8(1). 12-15 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Lazarus, M., et al.: "Biochemical characterization of mouse microsomal prostaglandin E synthase-1 and its colocalization with cyclooxygenase-2 in peritoneal macrophages."Arch.Biochem.Biophys.. 397(2). 336-341 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hayaishi, O.: "Unraveling the enigma of sleep-molecular mechanisms of sleep-wake regulation."Oxygen and Life -0xygenases, Oxidases and Lipid Mediators-. (Eds. by Ishimura, Y., Nozaki, M., Yamamoto, S., Shimizu, T., Narumiya, S., and Mitani, F., Elsevier, Amsterdam). 503-509 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Eguchi, N., et al.: "Sleep in transgenic and gene-knockout mice for lipocalin-type prostaglandin D synthase."Oxygen and Life -0xygenases, Oxidases and Lipid Mediators-.(Eds.by Ishimura, Y., Nozaki, M., Yamamoto, S., Shimizu, T., Narumiya, S., and Mitani, F., Elsevier, Amsterdam). 429-433 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Huang, Z.-L., et al.: "Prostaglandin E_2 activates the histaminergic system in the rat brain."Oxygen and Life -0xygenases, Oxidases and Lipid Mediators-.(Eds.by Ishimura, Y., Nozaki, M., Yamamoto, S., Shimizu, T., Narumiya, S., and Mitani, F., Elsevier, Amsterdam). 441-445 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Irikura, D., et al.: "Crystal structure of lipocalin-type prostaglandin D synthase."Oxygen and Life -0xygenases, Oxidases and Lipid Mediators-.(Eds.by Ishimura, Y., Nozaki, M., Yamamoto, S., Shimizu, T., Narumiya, S., and Mitani, F., Elsevier, Amsterdam). 453-459 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kubata, B.K., et al.: "Enzymatic formation of prostaglandin D_2, E_2, and F_<2α> in the parasitic protozoan Trypanosoma brucei."Oxygen and Life -0xygenases, Oxidases and Lipid Mediators-.(Eds.by Ishimura, Y., Nozaki, M., Yamamoto, S., Shimizu, T., Narumiya, S., and Mitani, F., Elsevier, Amsterdam). 461-466 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Qu WM, et al.: "A novel chronic pancreatitis model in MRL mice treated with Polyinosinic : Polycytidylic acid."Clin.Exp.Immunol.. 129(1). 27-34 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Urade, Y., Eguchi, N.: "Lipocalin-type and hematopoietic prostaglandin D synthases as a novel example of functional convergence."Prostaglandins & Other Lipid Mediators. 68-69. 375-382 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Taniike, M., et al.: "Perineuronal oligodendrocytes protect against neuronal apoptosis through the production of lipocalin-type prostaglandin D synthase in a genetic demyelinating model."J.Neurosci.. 22. 4885-4896 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Inui, T., et al.: "Characterization of the unfolding process of lipocalin-type prostaglandin D synthase."J.Biol.Chem.. 278. 2845-2852 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Irikura, D., et al.: "Cloning, expression, crystallization, and preliminary X-ray analysis of recombinant mouse lipocalin-type prostaglandin D synthase, a somnogen-producing enzyme."J.Biochem.(Tokyo). 133. 29-32 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Huang, Z.-L., et al.: "Prostaglandin E_2 activates the histaminergic system via the EP_4 receptor to induce wakefulness in rats."J.Neurosci.. 23(14). 5975-5983 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Okada, T., et al.: "Dominant localization of adenosine deaminase in leptomeninges and involvement of the enzyme in sleep."Biochem.Biophys.Res.Commun.. 312(1). 29-34 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Urade, Y., et al.: "Sleep regulation in adenosine A_<2A> receptor-deficient mice."Neurology. 61(Suppl 6). S94-S96 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hiraoka, A., et al.: "Bilirubin, biopyrrins, and β-trace protein(lipocalin-type prostaglandin D synthase) in the cerebrospinal fluid of patients with various neurological disorders."J.of Analytical Bio-Science. 26(3). 246-253

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Mohri, I., et al.: "Hematopoietic prostaglandin D synthase is expressed in microglia in the developing postnatal mouse brain."GLIA. 42. 263-274 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kameyama, M., et al.: "Effect of phospholipase Cβ4 lacking in thalamic neurons on electroencephalogram."Biochem Biophys Res Commun. 304. 153-159 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Urade, Y., et al.: "Functional analyses of lipocalin-type and hematopoietic prostaglandin D synthases."Folia Pharmacol.Jpn.. 123. 5-13 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Urade Y., et al.: "Molecular mechanisms of sleep."Clin.Neurosci.. 22(1). 37-40 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hayaishi, O., et al.: "Genes for prostaglandin D synthase and receptor as well as adenosine A_<2A> Receptor are involved in the homeostatic regulation of nrem sleep."Archives Italiennes de Biologie. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hayaishi, O.: "Molecular genetic studies on sleep-wake regulation, with special emphasis on the prostaglandin D2 system-."J.Appl.Physiol.. 92(2). 863-868 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hayaishi, O., Urade, Y.: "Prostaglandin D2 in sleep-wake regulation : recent progress and perspectives."Neuroscientist. 8(1). 12-15 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Lazarus, M., Kubata, B.K., Eguchi, N., Fujitani Y., Urade, Y., Hayaishi, O.: "Biochemical characterization of mouse microsomal prostaglandin E synthase-1 and its colocalization with cyclooxygenase-2 in peritoneal macrophages."Arch.Biochem.Biophys.. 397(2). 336-341 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Qu VIM, Miyazaki T, Terada M, Okada K, Mori S, Kanno H, Nose M.: "A novel chronic pancreatitis model in MRL mice treated with Polyinosinic : Polycytidylic acid."Clin Exp Immunol. 129(1). 27-34 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Urade, Y., Eguchi, N.: "Lipocalin-type and hematopoietic prostaglandin D synthases as a novel example of functional convergence"Prostaglandins & Other Lipid Mediators.. 68-69. 375-382 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Taniike, M., Mohri, I., Eguchi, N., Beuckmann, C.T., Suzuki, K., Urade, Y.: "Perineuronal oligodendrocytes protect against neuronal apoptosis through the production of lipocalin-type prostaglandin D synthase in a genetic demyelinating model."J.Neurosci.. 22. 4885-4896 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Inui, T., Okubo, T., Emi, M., Irikura, D., Hayaishi, O., Urade, Y: "Characterization of the unfolding process of lipocalin-type prostaglandin D synthase."J.Biol.Chem.. 278. 2845-2852 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Irikura, D., Kumasaka, T., Yamamoto, M., Ago, H., Miyano, M., Kubata, K.B., Sakai, H., Hayaishi, O., Urade, Y.: "Cloning, expression, crystallization, and preliminary X-ray analysis of recombinant, mouse lipocalin-type prostaglandin D synthase, a somnogen-producing enzyme."J.Biochem.(Tokyo). 133. 29-32 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Huang, Z.-L., Sato, Y, Mochizuki, T., Okada, T., Qu, W.-M., Yamatodani, A., Urade, Y., Hayaishi, O.: "Prostaglandin E2 activates the histaminergic system via the HP4 receptor to induce wakefulness in rats."J.Neurosci.. 23(14). 5975-5983 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Okada, T., Mochizuki, T., Huang, Z.-L., Eguchi, N., Sugita, Y, Urade, Y., Hayaishi, O.: "Dominant localization of adenosine deaminase in leptomeninges and involvement of the enzyme in sleep."Biochem.Biophys.Res.Commun.. 312(1). 29-34 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Urade, Y., Eguchi, N., Qu, W.-M., Sakata, M., Huang, Z.-L., Chen, J.-F., Schwarzschild, M.A., Fink, J.S., Hayaishi, O.: "Sleep regulation m adenosine A_<2A> receptor-deficient mice."Neurology. 61(Suppl 6)(3). 246-253 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hiraoka, A., Katoh, Y, Horii, H., Seiki, K., Oda, H., Eguchi, N., Urade, Y, Tominaga, I., Hori, K.: "Bilirubin, biopyrruis, and β-trace protein (lipocalin-type prostaglandin D synthase) in the cerebrospinal fluid of patients with various neurological disorders."J.of Analytical Bio-Science. 26(3). 246-253 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Mohri, I., Eguchi, N., Suzuki, K., Urade, Y, Taniike, M.: "Hematopoietic prostaglandin D synthase is expressed in microglia in the developing postnatal mouse brain."GLIA. 42. 263-274 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kameyama, M., Yamaguchi, I., Ichikawa, K., Sugiyama, T., Hori, H., Ikeda, M., Kuwahata, Y, Eguchi, N., Urade, Y, Yoshioka, T.: "Effect of phospholipase Cβ4 lacking in thalamic neurons on electroencephalogram."Biochem Biophys Res Commun. 304. 153-159 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Urade, Y., Eguchi, N., Aritake, K., Hayaishi O.: "Functional analyses of lipocalin-type and hematopoietic prostaglandin D synthases."Folia Pharmacol.Jpn.. 123. 5-13 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Urade Y, Eguchi N, Huang ZL, Qu WM.: "Molecular mechanisms of sleep."Clin Neurosci.. 22(1). 37-40 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hayaishi, O., Urade, Y, Eguchi, N., Huang, Zhi-Li.: "Genes for prostaglandin D synthase and receptor as well as adenosine A_<2A> Receptor are involved in the homeostatic regulation of nrem sleep."Archives Italiennes de Biologie. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hayaishi, O.: "Unraveling the enigma of sleep-molecular mechanisms of sleep-wake regulation. Oxygen and Life-Oxygenases, Oxidases and Lipid Mediators-.(Eds.by Ishimura, Y., Nozaki, M., Yamamoto, S., Shimizu, T., Narumiya, S., and Mitani, F)(Amsterdam)"Elsevier. 503-509 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Eguchi, N., Pinzar, B., Kuwahata, Y., Inui, T., Mochizuki, T., Urade, Y., Hayaishi, O.: "Sleep in transgenic and gene-knockout mice for lipocalin-type prostaglandin D synthase. Oxygen and Life-Oxygenases, Oxidases and Lipid Mediators-.(Eds.by Ishimura, Y., Nozaki, M., Yamamoto, S., Shimizu, T., Narumiya, S., and Mitani, F)(Amsterdam)"Elsevier. 429-433 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Huang, Z.-L, Mocihzuki, T., Okada, T., Hayaisi, O., Urade, Y.: "Prostaglandin the histaminergic system in the rat brain. Oxygen and Life-Oxygenases, Oxidases and Lipid Mediators-.(Eds.by Ishimura, Y., Nozaki, M., Yamamoto, S., Shimizu, T., Narumiya, S., and Mitani, F)(Amsterdam)"Elsevier. 441-445 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Irikura, D., Kumasaka, T., Yamamoto, M., Hayaishi, O., Urade, Y.: "Crystal structure of lipocalin-type prostaglandin D synthase. Oxygen and Life-Oxygenases, Oxidases and Lipid Mediators-.(Eds.by Ishimura, Y., Nozaki, M., Yamamoto, S., Shimizu, T., Narumiya, S., and Mitani, F)(Amsterdam)"Elsevier. 461-466 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kubata, B.K., Duszenko M., Kabututu, Z., Rawer, M., Szallies, A., Inui, T., Urade, Y., Hayaishi, O.: "Enzymatic formation of prostaglandin D_2, E_2, and F_2 in the parasitic protozoan Trypanosoma brucei."Elsevier.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Fujimori, K.: "Regulation of lipocalin-type prostaglandin D synthase gene expression by Hes-1 through E-box and interleukin-1 β via two NF-κB elements in rat leptomeningeal cells."J.Biol.Chem.. 278. 6018-6026 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Huang, Z.-L.: "Prostaglandin B2 activates the histaininergic system via the EP4 receptor to induce wakefulness in rats."J.Neurosci.. 23. 29-34 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Hayaishi, O.: "Oxygenases."Encyclopedia of Biological Chemistry. in press. 2845-2852 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Irikura, D.: "Cloning, Expression, Crystallization, and Preliminary X-Ray Analysis of Recombinant Mouse Lipocalin-type Prostaglandin D Synthase, a Somnogen-Producmg Enzyme"J. Biochem.. 133. 29-32 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Hayaishi, O.: "Prostaglandin D2 in Sleep-wake regulation : recent progress and perspectives"The Neuroscientist. 8. 12-15 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Hayaishi, O.: "Functional Genomics of Sleep and Circadian Rhythm Invited Revew : Molecular genetics studies on"

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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