| Project/Area Number |
14580759
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Neurochemistry/Neuropharmacology
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| Research Institution | National Center of Neurology and Psychiatry (NCNP) |
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Principal Investigator |
AKAZAWA Chihiro National Center of Neurology and Psychiatry (NCNP), National Institute of Neuroscience, Section Chief, 代謝研究部, 室長 (80291160)
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| Project Period (FY) |
2002 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2003: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 2002: ¥1,900,000 (Direct Cost: ¥1,900,000)
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| Keywords | RING-H2 domain / ubiguitin-proteasome / endosome-lysosome / synaptic transmission / syntaxin / yeast two hybrid / 神経細胞 / 小胞輸送 / エンドソーム / リソソーム / 細胞内局在 / シナプス / 伝達物質放出 / 酵母 |
|---|
| Research Abstract |
Mammalian homologues of Class C VPS proteins play an essential role in the trafficking between endosome to lysosome. We investigated the potential role of Class C VPS proteins in rat brain. We found that Class C VPS proteins are predominantly expressed in neurons of whole brain regions. To investigate the molecular function, we searched the interacting molecules by using yeast two hybrid screening. Interestingly, Class C VPS proteins were shown to interact with presynaptic SNARE molecues, such as syntaxin-1 and nSec-1/Munc 18. In the HIT cells cotransfected with Class C VPS proteins and hGH reporter gene, the high concentration of KC1 (25mM) treatment induced the secretion of hGH and this increases were inhibited by Ca-channel blocker, nifedipine. These results suggest that Class C VPS proteins may be involved in Ca-dependent vesicle releases through interacting with neuronal SNARE, syntaxin-1 and nSec-1/Munc 18.
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