| Project/Area Number |
14580760
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Neurochemistry/Neuropharmacology
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| Research Institution | National Institute of Advanced Industrial Science and Technology (AIST) |
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Principal Investigator |
KAMEYAMA Kimihiko National Institute of Advanced Industrial Science and Technology (AIST), Neuroscience Institute, Researcher, 脳神経情報研究部門, 研究員 (50224697)
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| Project Period (FY) |
2002 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2003: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2002: ¥2,200,000 (Direct Cost: ¥2,200,000)
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| Keywords | CaMKII / NMDA receptor / Protein phosphorylation / protein phosphatase / Calcium binding protein / CaMK II / 脱リン酸化 / フォスファターゼ |
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| Research Abstract |
Calcium-Calmodulin kinase II (CaMKII) is a key molecule to induce hippocampal long term potentiation. To find the interacting proteins to CaMKII at post synaptic membranes, I screened for brain cDNA library in yeast two hybrid system with point mutataed active form CaMKII and NMDA receptor 2B subunit's intracellular domain complex as bate. I found one clone which coded protein phosphatase 2A(PP2A) that interact with active CaMKII but not to inactive CaMKII. CaMKII, NMDA receptor and PP2A complex are found in heterologous expressed COS cells. I also found calcium binding protein calreticulin interact with CaMKII and NMDA receptor complex.
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