Molecular mechanism for the extension of neuronal growth cone : role of exocytosis of integrin.
| Project/Area Number |
14580769
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Neuroscience in general
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| Research Institution | nagoya university |
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Principal Investigator |
TATSUMI Hitoshi nagoya university, School of Medicine, Associate Professor, 大学院・医学系研究科, 助教授 (20171720)
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| Co-Investigator(Kenkyū-buntansha) |
SOKABE Masahiko nagoya university, School of Medicine, Professor, 大学院・医学系研究科, 教授 (10093428)
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| Project Period (FY) |
2002 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2003: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 2002: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Keywords | Growth cone / Contact / exocytosis / integrin / fluorescence imaging / near-field light / vesicle trafficking / 伝達物質 / エンドサイトシス / 生物物理 |
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| Research Abstract |
Adhesive contact at a growth cone has been reported in a variety of neurons and is thought to be crucial for neurite extension. We have recently demonstrated a close correlation between exocytosis of neurotransmitters and the formation of adhesive contacts at growth cones {Tatsumi & Katayama 1999 4371 /id}. Contact between a cell substrate and a growth cone was also enhanced when excitatory stimulations are applied to growth cones ^<49>. These studies suggest a close interrelation between an adhesive contact and vesicle localization at the growth cones. To examine the relationship between exocytosis and integrin incorporation into cell membrane, cells were stained with FM4-64 (vesicle marker for exocytosis) and an anti-integrin antibody labeled with Alexa488. Time lapse imaging of the stained cell demonstrated that the majority of vesicles contained both integrins and FM4-64. Analysis of the changes of integrins and FM4-64 fluorescence suggests that incorporation of integrins into the surface membrane of the growth cone after exocytosis, resulting in enhancement of contacts to substrate at growth cones.
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Report
(3 results)
Research Products
(12 results)
