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Interaction of three dimensional network of the feeding regulation neurons and steroid hormones

Research Project

Project/Area Number 14580772
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Neuroscience in general
Research InstitutionKyoto Prefectural University of Medicine

Principal Investigator

OZAWA Hitoshi  Kyoto Prefectural university of Medicine, Department of Anatomy and Neurobiology, Associate professor, 医学研究科, 助教授 (60169290)

Co-Investigator(Kenkyū-buntansha) NISHI Mayumi  Kyoto Prefectural University of Medicine, Department of Anatomy and Neurobiology, Assistant professor, 医学研究科, 講師 (40295639)
MATSUDA Ken-ichi  Kyoto Prefectural University of Medicine, Department of Anatomy and Neurobiology, Research Associate, 医学研究科, 助手 (40315932)
森田 規之  京都府立医科大学, 医学部, 講師 (50239662)
Project Period (FY) 2002 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥4,100,000 (Direct Cost: ¥4,100,000)
Fiscal Year 2004: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2003: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2002: ¥1,700,000 (Direct Cost: ¥1,700,000)
KeywordsOrexin / Estrogen / Sex difference / Neuropeptide Y(NPY) / Hypothalamus / Feeding / Immunohistochemistry / Confocal laser scanning microscopy / 共焦点レーザ顕微鏡 / orexin receptor / estrogen receptor / leptin receptor / ステロイドホルモン / 二重免疫組織化学 / NPYニューロン / POMCニューロン / orexinニューロン / 電子顕微鏡
Research Abstract

It has been reported that there are two orexin (from the Greek word for appetite, orexis) peptides derived from pre-pro-orexin, designated as orexin A and B. Orexin A is a 33-residue peptite with two intramolecular disulfide bonds in the N-terminal region, and orexin B is a linear 28-residue peptide. These peptides, encoded by a single mRNA transcript, has a 46% amino acid sequence identity (Sakurai T et al. 1998). Their effects are mediated by receptors, orexin 1 and 2 receptor. Orexin-A has ha similar affinity for both receptors, whereas orexin-B has a higher affinity for the orexin 2 receptor subtype.
It has been also reported that orexin-A and -B mRNA and their cell bodies were mainly detedted in the hypothalamic area, particularly in the lateral hypothalamic area and the perifornical nucleus. Recently, it has been reported that the distribution of immunoreactive fibers overlaps with the luteinizing hormone-releasing hormone(LHRH) neuronal system in the septopreoptic area and the ar … More cuate nucleus median eminence region, suggesting that orexins are involved with the sex hormone secretion. However, the sex difference of the expression of orexin-A and -B has not been elucidated. In the project, we investigated the sex difference of the distribution pattern of the feeding related neurons, particularly orexin neuorns and the effect of estrogen for the expression of orexin peptides in the male, female and ovariectomized (OVX) rats.
Orexin neurons are distributed in the perifornical and lateral hypothalamic area, a region classically implicated in the control of feeding behavior, and project their axons within the hypothalamus and throughout the central nervous system to be involved in the control of feeding. In the project, we investigated the sexual difference of orexin neurons using specific orexin-A and orexin-B antibodies. The density of both orexin-A and orexin-B immunopositive neurons in male was preferentially higher than those in female rats. On the other hand, ovariectomy in female rats induced the change to the male pattern. Colocalization of orexin and estrogen receptor was not observed. These results suggested that orexin neurons were indirectly regulated by estrogen through the other estrogen dependent neurons. Less

Report

(4 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • 2002 Annual Research Report
  • Research Products

    (24 results)

All 2004 2002 Other

All Journal Article (17 results) Book (1 results) Publications (6 results)

  • [Journal Article] Colocalization of mineralocorticoid receptor and glucocorticoid receptor in the hippocampus and hypothalamus.2004

    • Author(s)
      Han F, Ozawa H, Matsuda K, Nishi M, Kawata M.
    • Journal Title

      M.Neurosci Res (In press)

    • NAID

      10015451755

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Neurons expressing relaxin 3/INSL7 in the nucleus incertus respond to stress.2004

    • Author(s)
      Tanaka M, Iijima N, Miyamoto Y, Fukusumi S, Itoh Y, Ozawa H, Ibata Y.
    • Journal Title

      Eur J Neurosci (In press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Transgenic expression of enhanced green fluorescent protein enables direct visualization for physiological studies of vasopressin neurons and isolated nerve terminals of the rat.2004

    • Author(s)
      Ueta Y., Fujihara H., Serino R., Dayanithi G., Ozawa H., Matsuda K., Kawata M., Yamada J., Ueno S., Fukuda, A., Murphy D.
    • Journal Title

      Endocrinology 146

      Pages: 406-413

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Exocytosis sensitivity to growth hormone-releasing hormone in subsets of GH cells in rats under different corticosterone condition. Ultrastructural study using microwave irradiation for fixation2004

    • Author(s)
      Ozawa H., Han F., Kawata M.
    • Journal Title

      J Endocrinol 183

      Pages: 507-515

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Imaging analysis of subcellular correlation of androgen receptor and estrogen a in single living cells using GFP color variants2004

    • Author(s)
      Ochiai I., Matsuda K., Nishi M., Ozawa H., Kawata M.
    • Journal Title

      Mol Endocrinol 18

      Pages: 26-42

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Transgenic expression of enhanced green fluorescent protein enables direct visualization for physiological studies of vasopressin neurons isolated nerve terminals of the rat.2004

    • Author(s)
      Ueta Y., Fujiwara H., Serino R., Dayanithi G., Ozawa H., Matsuda K., Kawata M., Yamada J., Ueno S., Fukuda A., Murphy D.
    • Journal Title

      Endocrinology 146

      Pages: 406-413

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Exocytosis sensitivity to growth hormone-releasing hormone in subsets of GH cells in rats under different corticosterone condition. Ultrastructural study using microwave irradiation for fixation and immunocytochemistry.2004

    • Author(s)
      Ozawa H., Han F., Kawata M.
    • Journal Title

      J Endocrinol 183

      Pages: 507-515

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Imaging analysis of subcellular correlation of androgen receptor and estrogen a in single living cells using GFP color varients.2004

    • Author(s)
      Ochiai I., Matsuda K., Nishi M., Ozawa H., Kawata M.
    • Journal Title

      Mol Endocrinol 18

      Pages: 26-42

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Colocalization of mineralocorticoid receptor and glucocorticoid receptor in the hippocampus and hypothalamus.2004

    • Author(s)
      Han F, Ozawa H, Matsuda K, Nishi M, Kawata M.
    • Journal Title

      M. Neurosci Res (In press)

    • NAID

      10015451755

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Neurons expressing relaxin 3/INSL7 in the nucleus incertus respond to stress.2004

    • Author(s)
      Tanaka M, Iijima N, Miyamoato Y, Fukusumi S, Itoh Y, Ozawa H, Ibata Y.
    • Journal Title

      Eur J Neurosci (In press)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Transgenic expression of enhanced green fluorescent protein enables direct visualization for physiological studies of vasopressin neurons isolated nerve terminals of the rat.2004

    • Author(s)
      Ueta Y., Fujiwara H., Serino R., Dayanithi G., Ozawa H.
    • Journal Title

      Endocrinology 146

      Pages: 406-413

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Imaging analysis of subcellular correlation of androgen receptor and estrogen a in single living cells using GFP color varients2004

    • Author(s)
      Ochiai I., Matsuda K., Nishi M., Ozawa H., Kawata M.
    • Journal Title

      Mol Endocrinol 18

      Pages: 26-42

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Steroid efects on secretion from subsets of lactotrophs : role of folliculo-stellate cells and annexin 1.2002

    • Author(s)
      Morris J.F., Christian H.C., Chapman L.P., Epton M.J., Buckingham J.C., Ozawa H., Nishi M., Kawata M.
    • Journal Title

      Arch Physiol Biochem 110

      Pages: 54-61

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Annexin-1(Lipocortin-1)-immunoreactivity in the folliculo-stellate cells of rat anterior pituitary : The effect of adrenalectomy and corticosterone treatment on its subcellular distribution.2002

    • Author(s)
      Ozawa H., Miyachi M., Tsuchiya S., Morris J.F., Kawata M.
    • Journal Title

      J.Neuroendocrinol 14

      Pages: 621-628

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] ストレスとグルココルチコイド受容体2002

    • Author(s)
      小澤一史, 謝 藏霞, 小川 弘, 西 真弓, 河田光博
    • Journal Title

      脳の科学 24

      Pages: 219-226

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Co-localization of mineralocorticoid receptor and glucocorticoid receptor in the hippocampus and hypothalamus.

    • Author(s)
      Han F, Ozawa H, Matsuda K, Nishi M, Kawata M.
    • Journal Title

      Neurosci Res (In press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Neurons expressing relaxin 3/INSL7 in the nucleus incertus respond to stress.

    • Author(s)
      Tanaka M, Iijima N, Miyamoato Y, Fukusumi S, Itoh Y, Ozawa H, Ibata Y.
    • Journal Title

      Eur J Neurosci (In press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Book] ケリーMRI解剖学2004

    • Author(s)
      小澤一史 他
    • Total Pages
      312
    • Publisher
      丸善出版株式会社
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Publications] Ochiai I, Matsuda K, Nishi M, Ozawa H, Kawata M: "Imaging analysis of subcellular correlation of androgen receptor and estrogen receptor α in single living cells using green fluorescent protein color variants"Molecullar Endocrinology. 18. 26-42 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] 小澤一史 他: "トートラ 人体の構造と機能"丸善出版株式会社. 1158 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Ozawa H, Miyachi M, Tsuchiya S, Morris JF, Kawata M: "Annexin-1(Lipocortin-1))-immunoreactivity in the folliculo-stellate cells of rat anterior pituitary : The effect of adrenalectomy and corticosterone treatment onit's subcellular distribution"J.Neuroendocrinology. 14. 621-628 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Morris JF, Christian HC, Chapman LP, Ozawa H, Nishi M, Kawata M: "Steroid effects on secression from subsets of lactotrophs : Role of folliculo-stellate cells and Annexin 1"Arch Physiol Biochem. 110. 54-61 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 小澤一史, 謝 藏霞, 小川 弘, 西 真弓, 河田光博: "ストレスとグルココルチコイド受容体"脳の科学. 24. 219-226 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 小澤一史 他: "病気の形態学"学際企画. 265 (2002)

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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