| Project/Area Number |
14580812
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Biomedical engineering/Biological material science
|
|---|
| Research Institution | Chiba University |
|---|
Principal Investigator |
ISHIDA Atsushi Chiba University, Graduate School of Medicines, Assistant, 大学院・医学研究院, 助手 (70334183)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
MIYAZAKI Masaru Chiba University, Graduate School of Medicine, Professor, 大学院・医学研究院, 教授 (70166156)
KOSEKI Haruhiko Chiba University, Graduate School of Medicine, Professor, 大学院・医学研究院, 教授 (40225446)
|
|---|
| Project Period (FY) |
2002 – 2003
|
| Project Status |
Completed (Fiscal Year 2003)
|
| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2003: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 2002: ¥1,700,000 (Direct Cost: ¥1,700,000)
|
|---|
| Keywords | Bone Marrow Cell / CD34 positive cells / Peripheral Blood MNC / Growth Factor / Artificial ECM / Fibronectin / PTFE / Tissue Engineering / VEGF |
|---|
| Research Abstract |
1.Determination of effective and simple cell-groups sorting for vessel regeneration from bone marrow cells : Aiming at clinical applications, based on recent other research data, G-CSF mobilized peripheral-blood mononuclear cells are thought to be substituted for "cell-groups for vessel regeneration from bone marrow cells" and used for experiment "2"
2.Determination of optimal conditions for making "vascular sheets" shortly In vitrocombining three inevitable elements, bone marrow cells, growth factors and artificial extracellular matrix (ECM) for vessel regeneration : Main theme of this study. Endothelial colonization of bone marrow derived CD34 positive cells in culture plates was observed in the media with type I & IV Collagen, Fibronectin, Vitronectin and Fibrinogen as ECM. It was obtained at 1st year that efficacy of Fibronectin was 5 times of those of other ECM. At 2nd year, aiming at further ex vivo vessel regeneration with 3 dimensional culture, one of clinically used vascular prosthesis, PTFE (Polytetrafluoroethylene) sheets, were used for cell culture "scaffold" after coating with PAU (Polyaminourethane) to make hydrophilic and to increase tissue affinity, Bone marrow derived CD34 positive cells and G-CSF mobilized peripheral-blood mononuclear cells were used. Six ECM, type I & IV Collagen, Fibronectin, 2 Fibronectin peptide and Vitronectin were used for observation of acceleration of cell adhesion and proliferation in 3 dimensional culture system Among them, Fibronectin RGD peptide was most efficient.
3.To analyze the mechanism of transdifferenciation between endothelial cells and smooth muscle cells in "vascular sheets" It remains to be unclear.
|
