| Project/Area Number |
14580827
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | Jichi Medical University |
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Principal Investigator |
YAMAGAMI Hiroko JICHI MEDICAL SCHOOL, MEDICIN, ASSISTANT PROFESSOR, 医学部, 講師 (50216686)
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| Co-Investigator(Kenkyū-buntansha) |
YAMATO Masayuki TOKYO WOMENS MEDICAL UNIVERSITY, MEDICIN, ASSOSIATE PROFESSOR, 医学部, 助教授 (40267117)
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| Project Period (FY) |
2002 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2004: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2003: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2002: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Keywords | Corneal endothelial transplantation / Cell Culture / Mouse / Rat |
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| Research Abstract |
The corneal endothelial cells are very important for clear vision. But, they have limited to increase after birth. When the numbers of corneal endothelial cells decrease by corneal dystrophies, inflammation and/or injury, the cornea became edema. That causes corneal opacification and visual loss and need the corneal transplantation. The donor corneas are insufficient in Japan and we need to get more corneas.
Institute of Biomedical Engineering, Tokyo Women's Medical University have previously developed a temperature-responsive cell culture surface by grafting poly(N-isopropylacrylamide)(PIPAAm) that changes its surface hydrophobicity in response to temperature. While this surface shows similar hydrophobicity to that of commercial polystyrene cell culture surfaces and facilitates cell adhesion and proliferation at 37 degrees C, grafted polymer becomes hydrophilic below 32 degrees C and releases spread cultured cells without trypsin.
The corneal endothelial cells from SD rat or Balb/c mouse were cultured to confluency at 37 degrees C on these surfaces. When the culture temperature was reduced below 32 degrees C, cells detached from the PIPAAm-grafted areas without any need for trypsin. This Corneal sheet was harvested intact simply by reducing the temperature, without the use of proteases. Cell-cell junctions and extracellular matrix on the basal side of the sheet, critical to sheet integrity and function, remained intact. We tried to use the medium without FBS. Corneal transplantation performed for mouse using the Penetrating Keratoplasty technique (allogenic endothelium and autogenic stroma).
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