• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

The Molecular Mechanism of Organ Regression during the Development

Research Project

Project/Area Number 14599007
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 細胞死(アポトーシス)
Research InstitutionHiroshima University

Principal Investigator

YAOITA Yoshio  Hiroshima University, Graduate School of Science, Professor, 大学院・理学研究科, 教授 (00166472)

Co-Investigator(Kenkyū-buntansha) NAKAJIMA Keisuke  Hiroshima University, Graduate School of Science, Research Associate, 大学院・理学研究科, 助手 (60260311)
Project Period (FY) 2002 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2004: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2003: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2002: ¥1,900,000 (Direct Cost: ¥1,900,000)
Keywordsmetamorphosis / programmed cell death / apoptosis / thyroid hormone / thyroid hormone receptor / organ regression / matrix metalloproteinase / Xenopus
Research Abstract

Many organs are formed in the process of the development, while unnecessary ones are eliminated. During the amphibian metamorphosis, tadpole tail and gills are regressing, as levels of plasma thyroid hormone are rising. By our research the following results are obtained.
1.We injected dominant-negative thyroid hormone receptor gene together with a maker gene into tail muscle cells in living tadpoles to interrupt thyroid hormone signaling, thereby demonstrating that muscle cell death in tadpole tail commit suicide till NF-stage 62. Furthermore, we have shown that muscle cell death in rapidly shortening tails after NF-stage 62 is executed not only by suicide, but also by murder mechanism where cells lose the anchorage and die by the degradation of the extracellular matrix due to the increase of the matrix metalloproteinases.
2.The matrix metalloproteinases(MMP) are believed to play a main role in the murder mechanism during the tail regression. A MMP inhibitor represses completely the resolution of the notochord that is constituted by the basement membrane, but partially the regression of tail. This result supports that dual mechanisms, suicide and murder, contribute to tail regression.
3.We have isolated two clones by the subtraction library and differential hybridization as genes correlated with tail muscle cell death, obtained their full-length cDNAs, and analyzed. Both of them are actin-binding proteins, and not considered as suicide genes, because cell survival was not reduced by their overexpression in tadpole tail-derived myoblastic cell line.
4.A cDNA library has been constructed by placing cDNAs downstream of the tetracycline-inducible promoter. Tadpoles are coinjected with clones of the cDNA library and a reporter gene, and reared in the presence of tetracycline derivative, doxycycline. We are searching for cDNA clones which diminishes the expression of reporter gene after doxycycline treatment.

Report

(4 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • 2002 Annual Research Report
  • Research Products

    (14 results)

All 2005 2004 2003 2002 Other

All Journal Article (11 results) Publications (3 results)

  • [Journal Article] Programmed cell death during amphibian metamorphosis.2005

    • Author(s)
      Keisuke Nakajima
    • Journal Title

      Seminars in Cell and Developmental Biology 16

      Pages: 271-280

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] 両生類変態の分子機構2005

    • Author(s)
      中島 圭介
    • Journal Title

      タンパク質核酸酵素 50

      Pages: 717-723

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Molecular mechanisms of amphibian metamorphosis2005

    • Author(s)
      Keisuke Nakajima
    • Journal Title

      Potein, Nucleic Acid and Enzyme 50

      Pages: 717-723

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Programmed cell death during amphibian metamorphosis.2005

    • Author(s)
      K.Nakajima, K.Fujimoto, Y.Yaoita
    • Journal Title

      Seminars in Cell and Developmental Biology (In press)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] 両生類変態の分子機構2005

    • Author(s)
      中島圭介, 矢尾板芳郎
    • Journal Title

      タンパク質核酸酵素 50卷6号

      Pages: 717-723

    • Related Report
      2004 Annual Research Report
  • [Journal Article] The adaptor molecule FADD from Xenopus laevis demonstrates evolutionary conservation of its pro-apoptotic activity.2004

    • Author(s)
      Kazuhiro Sakamaki
    • Journal Title

      Genes to Cells 9

      Pages: 1249-1264

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Involvement of caspase-9 in execution of the maternal program of apoptosis in Xenopus late blastulae overexpressed with SAMDC (S-adenosylmethionine decarboxylase).2004

    • Author(s)
      Eiji Takayama
    • Journal Title

      Biochem.Biophys.Res.Commun. 325

      Pages: 1367-1375

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] The adaptor molecule FADD from Xenopus laevis demonstrates evolutionary conservation of its pro-apoptotic activity.2004

    • Author(s)
      Kazuhiro Sakamaki, その他
    • Journal Title

      Genes to Cells 9

      Pages: 1249-1264

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Involvement of caspase-9 in execution of the maternal program of apoptosis in Xenopus late blastulae overexpressed with SA2004

    • Author(s)
      Eiji Takayama, その他
    • Journal Title

      Biochem.Biophys.Res.Commun. 325

      Pages: 1367-1375

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Dual mechanisms governing muscle cell death in tadpole tail during amphibian metamorphosis.2003

    • Author(s)
      Keisuke Nakajima
    • Journal Title

      Developmental Dynamics 227

      Pages: 246-255

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] The inhibition of the nuclear transport of caspase-7 by its prodomain.2002

    • Author(s)
      Yoshio Yaoita
    • Journal Title

      Biochem.Biophys.Res.Commun. 291

      Pages: 79-84

    • NAID

      120000875317

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Publications] K.Nakajima, Y.Yaoita: "Dual mechanisms governing muscle cell death in tadpole tail during amphibian metamorphosis"Developmental Dynamics. 227. 246-255 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Y.Yaoita: "The inhibition of the nuclear transport of caspase-7 by its prodomain"Biochem.Biophys.Res.Commun.. 291. 79-84 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] K.Nakajima, Y.Yaoita: "Dual mechanisms governing muscle cell death in tadpole tail during amphibian metamorphosis"Developmental Dynamics. (in press). (2003)

    • Related Report
      2002 Annual Research Report

URL: 

Published: 2002-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi