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皮膚におけるウイルス潜伏感染機序の解明

Research Project

Project/Area Number 14657200
Research Category

Grant-in-Aid for Exploratory Research

Allocation TypeSingle-year Grants
Research Field Dermatology
Research InstitutionOkayama University

Principal Investigator

岩月 啓氏  岡山大学, 大学院・医歯学総合研究科, 教授 (80126797)

Co-Investigator(Kenkyū-buntansha) 浅越 健治  岡山大学, 医学部・歯学部附属病院, 助手 (80294461)
大野 貴司  岡山大学, 医学部・歯学部附属病院, 講師 (80203884)
Project Period (FY) 2002 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 2003: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2002: ¥1,400,000 (Direct Cost: ¥1,400,000)
Keywordsγヘルペスウイルス / 潜伏感染 / EBウイルス / virokine / viral IL-10 / bcl-2 / EBNA-1 / EBウイルス感染症 / 持続感染 / ヴァイロカイン / 免疫回避
Research Abstract

γヘルペスウイルス潜伏感染のメカニズムを解析するために患者から樹立したEBウイルスNK/T細胞クローンを用いて以下の検討を実施した.
1.潜伏感染様式の解析:患者の末梢血からEBウイルス感染NK細胞株(NOY-1,NOY-2)とT細胞株(TOY-03)を樹立した.これらのEBウイルス感染NK/T細胞は,EBNA遺伝子のQpプロモーターを使用してEBNA1を発現し,EBNA-1とLMPを発現しているが,そのほかのウイルス抗原の発現は制限されていた(Latency II型).これらの細胞株を用いてTPAやTNF-α刺激を行い潜伏感染から溶解感染への移行を調べたが,明らかな移行は認められなかった.
2.Virokineの発現:EBウイルスがコードするviral IL-10およびbcl-2を発現するNK細胞腫瘍が認められた.これらのウイルス由来産物は宿主の免疫反応を制御し,細胞死を抑えることによって細胞内でのEBウイルス潜伏感染を維持するために好都合であると考えられた.
3.潜伏感染関連遺伝子発現制御の試み:mutant EBNA-1を細胞導入することによって,wtEBNA-1発現が低下した(高知大学医学部微生物学教室との研究,論文投稿中).潜伏感染ウイルス排除の新治療法のプロトタイプになり得る.
4.伝染性軟属腫ウイルス(MCV)持続感染についての解析:治療目的で採取した伝染性軟属腫組織からmRNAを抽出し,cDNAを作成した.MCVがコードする数種類のケモカイン,ケモカイン受容体の発現をPCR法で増幅した.結果を解析中である.

Report

(2 results)
  • 2003 Annual Research Report
  • 2002 Annual Research Report
  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] Oono T, Huh W-K, Shirafuji Y, Akiyama H, Iwatsuki K: "Localization of human beta-defensin -2 and human neutrophil peptides in superficial follicultis"Brit J Dermatol. 148. 171-192 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Akiyama H, Yamasaki O, Oono T, Fujimoto W, Iwatsuki K, et al.: "Confocal laser scanning microscopic observation of glycocalyx production by Staphylococcus aureus in skin lesions of bullous impetigo, atopic dermatitis and pemphigus foliaceus"Brit J Dermatol. 148. 526-532 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Tojo M, Kiyosawa H, Iwatsuki K, Nakamura K, Kaneko F: "Expression of the GL12 oncogene and its isoforms in human basal cell carcinoma"Brit J Dermatol. 148. 892-897 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Akiyama H, Morizane S, Yamasaki O, Oono T, Iwatsuki K: "Assessment of Streptococcus pyogenes microcolony formation in infected skin by confocal laser scanning microscopy"J Dermatol Sci. 32. 193-199 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Katagiri Y, Mitsuhashi Y, Kondo S, Kanazawa C, Iwatsuki K, et al.: "Hydroa vacciniforme-like eruptions in a patient with chronic active EB virus infection"J Dermatol. 30. 400-404 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kunisada M, Adachi A, Matsumot S, Ogawa Y, Horikawa T, Iwatsuki K: "Nasal-type natural killer cell lymphoma preceded by benign panniculitis arising in an asymptomatic HTLV-1 carrier"Int J Dermatol. 42. 710-714 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 岩月啓氏: "EBウイルスと皮膚疾患,種痘様水疱症,蚊刺過敏症(最新皮膚科学大系第15巻ウイルス性疾患性感染症,16巻動物性皮膚症環境因子による皮膚障害)"中山書店,東京. 11 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Oono T, Shirafuji Y, Huh W-K, Akiyama H, Iwatsuki K: "Effects of human neutrophil peptide-1 on the expression of interstitial collagenase and type I collagen in human dermal fibroblasts"Arch Dermatol Res. 294. 185-189 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Iwatsuki K, et al.: "Dynamic alteration of human beta-defensin 2 localization from cytoplasm to intercellular space in psoriatic skin"J Mol Med. 80. 678-684 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Iwatsuki K, Xu Z-G, Ohtsuka M, Yamamoto T, Fujii K, Tsuji K: "Photosensitivity in hydro a vacciniforme is a photo-Koebner phenomenon induced by infiltration of Epstein-Barr virus-infected NK/T cells"J Photosci. 9. 233-235 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Iwatsuki K, Ohtsuka M, Matsui T, Yamamoto T, Fujii K, Yamasaki O, Tsuji K: "Erythrodermic actinic reticuloid is characterized by the overspill of oligoclonal CD8+ lymphocytes responsive to ultraviolet irradiation"J Photosci. 9. 491-493 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Tojo M, Iwatsuki K, Furukawa H, Takahashi M, Kaneko F: "Neutrophilic eccrine hidradenitis in actinic reticuloid syndrome"Eur J Dermatol. 12. 198-200 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Satoh M, Oyama N, Akiba H, Ohtsuka M, Iwatsuki K, Kaneko F: "Hypersensitivity to mosquito bites with natural -killer cell lmphocytosis : the possible implication of Epstein-Barr virus reactivation"Eur J Dermatol. 12. 381-384 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 岩月啓氏: "最新皮膚科学大系"中山書店. 329-333 (2002)

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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