ウシ小型ピロプラズマ病における貧血病態と発症機序の解明

• Project/Area Number: 14F04087
• Research Category: Grant-in-Aid for JSPS Fellows
• Allocation Type: Single-year Grants
• Section: 外国
• Research Field: Veterinary medical science
• Research Institution: Obihiro University of Agriculture and Veterinary Medicine
• Principal Investigator: 横山 直明 帯広畜産大学, 原虫病研究センター, 教授 (80301802)
• Co-Investigator(Kenkyū-buntansha): SIVAKUMAR THILLAIAMPALAM 帯広畜産大学, 原虫病研究センター, 外国人特別研究員 ; SIVAKUMAR Thillaiampalam 帯広畜産大学, 原虫病研究センター, 外国人特別研究員
• Project Period (FY): 2014-04-25 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥2,400,000 (Direct Cost: ¥2,400,000); Fiscal Year 2015: ¥1,200,000 (Direct Cost: ¥1,200,000); Fiscal Year 2014: ¥1,200,000 (Direct Cost: ¥1,200,000)
• Keywords: 小型ピロプラズマ病 / 牛 / 貧血 / 発症 / 原虫 / タイレリア

Outline of Annual Research Achievements

最終年度では、以下の研究成果を得た。
１）小型ピロプラズマ感染による放牧牛の貧血病態は宿主免疫の異常バランスに起因する大球性高色素性貧血であることを解明した。
２）スリランカ、エジプト、ベトナム、モンゴル、及びタイにおける牛やマダニのピロプラズマ感染の実態を国際疫学調査によって明らかにした。
３）ピロプラズマ病の治療薬の候補を同定した。

Research Progress Status

27年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

27年度が最終年度であるため、記入しない。

Research Products

• [Int'l Joint Research] 獣医学研究所(スリランカ)
• [Int'l Joint Research] モンゴル農業大学(モンゴル)
• [Int'l Joint Research] フエ大学/フエ農林大学(ベトナム)
• [Int'l Joint Research] サダト大学(エジプト)
• [Int'l Joint Research] 国立動物科学研究所(タイ)
• [Journal Article] Type-specific PCR assays for Babesia bovis msa-1 genotypes in Asia: Revisiting the genetic diversity in Sri Lanka, Mongolia, and Vietnam. (2016)
  • Author(s): Liyanagunawardena N, Sivakumar T, Kothalawala H, Silva SS, Battsetseg B, Lan DT, Inoue N, Igarashi I, Yokoyama N.
  • Journal Title: Infect Genet Evol. Volume: 37 Pages: 64-69
  • DOI: 10.1016/j.meegid.2015.10.029
  • NAID: 120006390924
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] The PCR detection and phylogenetic characterization of Babesia microti in questing ticks in Mongolia. (2015)
  • Author(s): Tuvshintulga B, Sivakumar T, Battsetseg B, Narantsatsaral SO, Enkhtaivan B, Battur B, Hayashida K, Okubo K, Ishizaki T, Inoue N, Igarashi I, Yokoyama N.
  • Journal Title: Parasitol Int. Volume: 64 Issue: 6 Pages: 527-532
  • DOI: 10.1016/j.parint.2015.07.007
  • NAID: 120006390873
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Genetic variations in merozoite surface antigen genes of Babesia bovis detected in Vietnamese cattle and water buffaloes. (2015)
  • Author(s): Yokoyama N, Sivakumar T, Tuvshintulga B, Hayashida K, Igarashi I, Inoue N, Long PT, Lan DT.
  • Journal Title: Infect. Genet. Evol. Volume: 30 Pages: 288-295
  • DOI: 10.1016/j.meegid.2014.12.035
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Genetic diversity in Trypanosoma theileri from Sri Lankan cattle and water buffaloes. (2015)
  • Author(s): Yokoyama N, Sivakumar T, Fukushi S, Tattiyapong M, Tuvshintulga B, Kothalawala H, Silva SS, Igarashi I, Inoue N.
  • Journal Title: Vet Parasitol. Volume: 207 Issue: 3-4 Pages: 335-41
  • DOI: 10.1016/j.vetpar.2014.12.006
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] An epidemiological survey of bovine Babesia and Theileria parasites in cattle, buffaloes, and sheep in Egypt. (2015)
  • Author(s): Elsify, A., Sivakumar, T., Nayel, M., Salama, A., Elkhtam, A., Rizk, M., Mosaab, O., Sultan, K., Elsayed, S., Igarashi, I., and Yokoyama, N.
  • Journal Title: Parasitol. Int. Volume: 64 Issue: 1 Pages: 79-85
  • DOI: 10.1016/j.parint.2014.10.002
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Evaluation of inhibitory effect of methylene blue against Babesia and Theileria parasites. (2015)
  • Author(s): Tuvshintulga B, Sivakumar T, Salama AA, Yokoyama N, Igarashi I.
  • Journal Title: J. Protozool. Res. Volume: 25 Pages: 18-28
  • NAID: 120006391034
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] First detection of Babesia venatorum (EU1) in Ixodes persulcatus ticks in Mongolia. (2015)
  • Author(s): Tuvshintulga B, Battsetseg B, Battur B, Myagmarsuren P, Narantsatsral S, Sivakumar T, Takemae H, Igarashi I, Inoue N, Yokoyama N.
  • Journal Title: J. Protozool. Res. Volume: 25 Pages: 29-37
  • NAID: 120006391035
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Analysis of the genetic diversity of Babesia bovis msa-1 in Thailand using type-specific polymerase chain reaction. (2015)
  • Author(s): Tattiyapong M, Sivakumar T, Takemae H, Simking P, Jittapalapong S, Igarashi I, Yokoyama N.
  • Journal Title: J. Protozool. Res. Volume: 25 Pages: 38-43
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Evolution and genetic diversity of Theileria. (2014)
  • Author(s): Sivakumar T, Hayashida K, Sugimoto C, Yokoyama N.
  • Journal Title: Infect. Genet. Evol. Volume: 27 Pages: 250-263
  • DOI: 10.1016/j.meegid.2014.07.013
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Genetic characterization of Babesia and Theileria parasites in water buffaloes in Sri Lanka. (2014)
  • Author(s): Sivakumar T, Tattiyapong M, Fukushi S, Hayashida K, Kothalawala H, Silva SS, Vimalakumar SC, Kanagaratnam R, Meewewa AS, Suthaharan K, Puvirajan T, de Silva WK, Igarashi I, Yokoyama N.
  • Journal Title: Vet. Parasitol. Volume: 200 Issue: 1-2 Pages: 24-30
  • DOI: 10.1016/j.vetpar.2013.11.029
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] Type-specific PCR assays for Babesia bovis msa-1 genotypes in Asia: Revisiting the genetic diversity in Sri Lanka, Mongolia, and Vietnam. (2015)
  • Author(s): Sivakumar T, Inoue N, Igarashi I, Yokoyama N.
  • Organizer: 第56回日本熱帯医学会大会
  • Place of Presentation: 大阪大学（大阪府吹田市）
  • Year and Date: 2015-12-04
• [Presentation] Molecular epidemiology of Babesia and Theileria, and Trypanosoma in cattle and water buffaloes in Sri Lanka. (2015)
  • Author(s): Sivakumar T, Kothalawala H, Silva SSP, Yokoyama N.
  • Organizer: 57th Annual Convention of the Sri Lanka Veterinary Association
  • Place of Presentation: Oak-Ray and Earl's Regency (Kandy, Sri Lanka)
  • Year and Date: 2015-07-19
  • Int'l Joint Research
• [Presentation] Genetic diversity of Theileria (2014)
  • Author(s): 横山直明、Sivakumar Thillaiamapalam, 林田京子、杉本千尋
  • Organizer: 第157回日本獣医学会学術集会
  • Place of Presentation: 北海道大学（札幌）
  • Year and Date: 2014-09-09 – 2014-09-12
• [Remarks] 帯広畜産大学・原虫病研究センター ホームページ
  • URL: http://www.obihiro.ac.jp/~protozoa/index.html