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合成微小環境を用いた軟骨組織のin vitro構築

Research Project

Project/Area Number 14F04106
Research Category

Grant-in-Aid for JSPS Fellows

Allocation TypeSingle-year Grants
Section外国
Research Field Dental engineering/Regenerative dentistry
Research InstitutionOkayama University

Principal Investigator

松本 卓也  岡山大学, 医歯(薬)学総合研究科, 教授 (40324793)

Co-Investigator(Kenkyū-buntansha) HARA EMILIO  岡山大学, 医歯(薬)学総合研究科, 外国人特別研究員
HARA Emilio  岡山大学, 医歯(薬)学総合研究科, 外国人特別研究員
Project Period (FY) 2014-04-25 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 2015: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2014: ¥1,200,000 (Direct Cost: ¥1,200,000)
Keywords軟骨 / 合成微小環境 / DNAメチル化 / 石灰化
Outline of Annual Research Achievements

The purpose of this study was to identify new methods and culture conditions to enhance chondrogenic differentiation of human bone marrow-derived mesenchymal stem/progenitor cells (hBMSCs) by applying chemical, physical and biological cues, including overexpression of DNA methyltransferases (DNMTs) in cells. We attempted to fabricate a chemically- and physically-controlled microenvironment using materials (e.g., hydrogels) that recapitulate native cell niche characteristics to optimally modulate the chondrogenic differentiation of BMSCs. Besides the physical and chemical stimulations, we also evaluated the chondrogenic differentiation of hBMSCs after overexpression of DNMTs that eventually induce DNA methylation at CPG rich regions of key genes. To study the effect of DNMTs on chondrogenesis of hBMSCs, we transfected overexpression vectors of DNMT3A, DNMT3B and the pcDNA control in hBMSCs, and culture the cells in a 3D micromass culture system with growth factors and glucocorticoids.
Additionally, since in vitro synthesized cartilage tissue generally undergoes through mineralization, we also attempted to understand the mechanisms involved in the chondrocyte death associated with initial mineralization process by using the secondary ossification center of mouse femur as in vivo model.

Research Progress Status

27年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

27年度が最終年度であるため、記入しない。

Report

(2 results)
  • 2015 Annual Research Report
  • 2014 Annual Research Report
  • Research Products

    (15 results)

All 2016 2015 2014 Other

All Int'l Joint Research (1 results) Journal Article (7 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 7 results,  Open Access: 6 results,  Acknowledgement Compliant: 3 results) Presentation (7 results) (of which Int'l Joint Research: 2 results)

  • [Int'l Joint Research] Harvard Univerisity(米国)

    • Related Report
      2015 Annual Research Report
  • [Journal Article] Antagonistic Effects of Insulin and TGF-β3 during Chondrogenic Differentiation of Human BMSCs under a Minimal Amount of Factors.2016

    • Author(s)
      Emilio Satoshi Hara, Ono Mistuaki, Yoshioka Yuya, Ueda Junji, Hazehara Yuri, Hai Thanh Pham, Matsumoto Takuya, Kuboki Takuo.
    • Journal Title

      Cells Tissues Organs

      Volume: 201 Issue: 2 Pages: 88-96

    • DOI

      10.1159/000442411

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Fluocinolone acetonide is a potent synergistic factor of TGF-β3-associated chondrogenesis of bone marrow-derived mesenchymal stem cells for articular surface regeneration2015

    • Author(s)
      Emilio Satoshi Hara, Mitsuaki Ono, Pham Thanh Hai, Wataru Sonoyama, Satoshi Kubota, Masaharu Takigawa, Takuya Matsumoto, Marian F. Young, Bjorn R. Olsen, Takuo Kuboki
    • Journal Title

      J. Bone Miner. Res.

      Volume: 未定 Issue: 9 Pages: 1585-1596

    • DOI

      10.1002/jbmr.2502

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Mesenchymal stem/progenitor cell isolation from tooth extraction sockets2014

    • Author(s)
      Ryu Nakajima, Mitsuaki Ono, Emilio Satoshi Hara, Yasutaka Oida, Shigehiko Shinkawa, Hai Thanh Pham, Kentaro Akiyama, Wataru Sonoyama, Kenji Maekawa, Takuo Kuboki
    • Journal Title

      Journal of Dental Research

      Volume: 93 Issue: 11 Pages: 1133-1140

    • DOI

      10.1177/0022034514549377

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Comparison of platelet serotonin transporter activity in subjects with severe sleep bruxism and control2014

    • Author(s)
      Hajime Minakuchi, Chiharu Sogawa C, Emilio Satoshi Hara, Haruna Miki, Kenji Maekawa, Norio Sogawa, Shigeo Kitayama, Yoshizo Matsuka, Glenn T Clark, Takuo Kuboki
    • Journal Title

      Journal of Prosthodontic Research

      Volume: 58 Issue: 4 Pages: 217-222

    • DOI

      10.1016/j.jpor.2014.06.003

    • NAID

      130004714111

    • ISSN
      1883-1958, 1883-9207
    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] A short-term treatment with tumor necrosis factor-alpha enhances stem cell phenotype of human dental pulp cells2014

    • Author(s)
      Mayu Ueda, Takuo Fujisawa, Mitsuaki Ono, Emilio Satoshi Hara, Hai Thanh Pham, Ryu Nakajima, Wataru Sonoyama, Takuo Kuboki
    • Journal Title

      Stem Cell Research & Therapy

      Volume: 5 Issue: 1 Pages: 31-31

    • DOI

      10.1186/scrt420

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] The regenerative effects of CCN2 independent modules on chondrocytes in vitro and osteoarthritis models in vitro2014

    • Author(s)
      Tarek Abd El Kader, et al.
    • Journal Title

      Bone

      Volume: 59 Pages: 180-188

    • DOI

      10.1016/j.bone.2013.11.010

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Efficient bone formation in a swine socket lift model using Escherichia coli-derived recombinant human bone morphogenetic protein-2 adsorbed in β-tricalcium phosphate.2014

    • Author(s)
      Mitsuaki Ono, Wataru Sonoyama, Katushi Yamamoto, Yasutaka Oida, Kentaro Akiyama, Shigehiko Shinkawa, Ryu Nakajima, Hai Than Pham, Emilio Satoshi Hara, Takuo Kuboki
    • Journal Title

      Cells, Tissues, Organs

      Volume: 199 Issue: 4 Pages: 249-255

    • DOI

      10.1159/000369061

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] Fluocinolone acetonide is a potent synergistic factor of TGF-β3-associated chondrogenesis of bone marrow-derived mesenchymal stem cells for articular surface regeneration.2016

    • Author(s)
      Hara ES, Ono M, Pham HT, Sonoyama W, Kubota S, Takigawa M, Matsumoto T, Young MF, Olsen BR, Kuboki T.
    • Organizer
      第29回日本軟骨代謝学会
    • Place of Presentation
      広島
    • Year and Date
      2016-02-20
    • Related Report
      2015 Annual Research Report
  • [Presentation] Potent cartilage regeneration with fluocinolone acetonide and TGF-β3.2015

    • Author(s)
      Hara ES, Ono M, Pham HT, Kubota S, Takigawa M, Young MF, Olsen BR, Matsumoto T, Kuboki T.
    • Organizer
      第37回日本バイオマテリアル学会大会
    • Place of Presentation
      京都
    • Year and Date
      2015-11-09
    • Related Report
      2015 Annual Research Report
  • [Presentation] A high-throughput screening identified fluocinolone acetonide as a potent synergistic factor of TGF-β3-mediated chondrogenesis of BMSCs for articular surface repair.2015

    • Author(s)
      Hara ES, Ono M, Pham HT, Sonoyama W, Kubota S, Takigawa M, Young MF, Olsen BR, Matsumoto T, Kuboki T.
    • Organizer
      American Society for Bone and Mineral Research Annual Meeting
    • Place of Presentation
      シアトル, USA
    • Year and Date
      2015-10-12
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] A Novel Method to Induce Strong Chondrogenesis of BMSCs and Promote Articular Surface Repair using Fluocinolone Acetonide and TGF-β3.2015

    • Author(s)
      Hara ES, Ono M, Pham HT, Sonoyama W, Kubota S, Takigawa M, Young MF, Olsen BR, Matsumoto T, Kuboki T.
    • Organizer
      Biomedical Engineering Society Annual Meeting
    • Place of Presentation
      タンパ, USA
    • Year and Date
      2015-10-08
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Qualitative analysis of tissue mineralization during secondary ossification of mouse femur2014

    • Author(s)
      Emilio Satoshi Hara, Masahiro Okada, Takako Hattori, Satoshi Kubota, Takuo Kuboki, TakuyaMatsumoto
    • Organizer
      第36回日本バイオマテリアル学会大会
    • Place of Presentation
      東京
    • Year and Date
      2014-11-18 – 2014-11-19
    • Related Report
      2014 Annual Research Report
  • [Presentation] Novel insights into the mineralization process during secondary ossification of the cartilage2014

    • Author(s)
      Emilio Satoshi Hara, Masahiro Okada, Takako Hattori, Satoshi Kubota, Takuo Kuboki, Takuya Matsumoto
    • Organizer
      International Symposium 2014 - Foresight Program "Nano-Biomaterials and Regenerative Medicine"
    • Place of Presentation
      東京
    • Year and Date
      2014-10-08 – 2014-10-09
    • Related Report
      2014 Annual Research Report
  • [Presentation] Quantitative analysis of mineralized tissue formation during cartilage secondary ossification of mouse femur2014

    • Author(s)
      Emilio Satoshi Hara, Masahiro Okada, Takuya Matsumoto
    • Organizer
      第64回日本歯科理工学会学術講演会
    • Place of Presentation
      広島
    • Year and Date
      2014-10-05 – 2014-10-06
    • Related Report
      2014 Annual Research Report

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Published: 2015-01-22   Modified: 2024-03-26  

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