| Project/Area Number |
14F04764
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| Research Category |
Grant-in-Aid for JSPS Fellows
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| Allocation Type | Single-year Grants |
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| Section | 外国 |
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| Research Field |
Animal physiology/Animal behavior
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| Research Institution | The Graduate University for Advanced Studies |
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Principal Investigator |
蟻川 謙太郎 総合研究大学院大学, 先導科学研究科, 教授 (20167232)
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| Co-Investigator(Kenkyū-buntansha) |
PIRIH PRIMOZ 総合研究大学院大学, 先導科学研究科, 外国人特別研究員
PIRIH Primoz 総合研究大学院大学, 先導科学研究科, 外国人特別研究員
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| Project Period (FY) |
2014-04-25 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 2016: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2015: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2014: ¥500,000 (Direct Cost: ¥500,000)
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| Keywords | insect vision / angled sunbeam butterfly / intraspecific signaling / retinal histology / eyeshine / spectral sensitivity / spatial sensitivity / LED stimulation / retinal hisgology / angled sunbeam / visual signalling / laboratory rearing |
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| Outline of Annual Research Achievements |
A major advance this year was implemented in the telemicroscope setup, which has a spectrophotometer head and a side port. Two images from different focal planes and a spectrum from a defined region can be recorded simultaneously. We used the setup for hyperspectral imaging of eye reflectance and fluorescence, corneal/ tissue slide transmittance and for rhodopsin dynamics. The spectroscopy and dynamic pupil activation experiments on Curetis acuta revealed that the long wavelength rhodopsin peaks at 575 nm. Furthermore, by analyzing the pupils of individual ommatidia to spectral stimulation, we found five to six clusters of ommatidial types, consistent with three short-wavelength receptor classes. The method is certainly a promising tool for studying butterfly eyes with red eyeshine. We thus applied the method to several lepidopteran species including a skipper and two hawkmoths.
Another advance was in building light stimulation device using LEDs. We developed an image analysis method using the device for estimating pigment concentrations in a hyperspectral image stack. To validate the device as a biomedical tool, we measured blood oxygenation in the frog skin: Dr Pirih published the results (Belusic et al. Sci rep, 6:32012, 2016). We now have a two-register LED synthesizer with anamorphic optics and a depolarizer in Sokendai, which Dr Pirih assembled successfully during his extended stay for 2 months. This will be a powerful research tool in my laboratory in coming years.
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| Research Progress Status |
28年度が最終年度であるため、記入しない。
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| Strategy for Future Research Activity |
28年度が最終年度であるため、記入しない。
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