| Project/Area Number |
14F04775
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| Research Category |
Grant-in-Aid for JSPS Fellows
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| Allocation Type | Single-year Grants |
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| Section | 外国 |
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| Research Field |
Molecular biology
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| Research Institution | Institute of Physical and Chemical Research |
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Principal Investigator |
SHIN JAE・WOO 国立研究開発法人理化学研究所, ライフサイエンス技術基盤研究センター, ユニットリーダー (60553849)
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| Co-Investigator(Kenkyū-buntansha) |
LUGINBUEHL JOACHIM 国立研究開発法人理化学研究所, ライフサイエンス技術基盤研究センター, 外国人特別研究員
LUGINBUEHL Joachim 独立行政法人理化学研究所, ライフサイエンス技術基盤研究センター, 外国人特別研究員
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| Project Period (FY) |
2014-04-25 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 2016: ¥200,000 (Direct Cost: ¥200,000)
Fiscal Year 2015: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2014: ¥1,000,000 (Direct Cost: ¥1,000,000)
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| Keywords | Induced neurons / cell reprogramming / single cell sequencing / gene network / Cell Reprogramming / Single cell sequencing / Gene network |
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| Outline of Annual Research Achievements |
Single-cell RNA sequencing can disentangle highly diverse cell populations, and offers a way to unravel Transcriptional Regulatory Network (TRN) controlling cell-reprogramming. To this end, we generated a pool of 20 neuronal transcription factors (TFs) and sought to distinguish exogenous and endogenous TFs using single-cell RNAseq. Taking advantage of the dissimilarity of nucleotide sequences, we could identify exogenous and endogenous genes in induced neurons, and revealed a highly diverse subpopulation of induced neurons with defined gene signatures. We plan to perform validation experiments in collaboration with BSI/RIKEN. These results will be published within this fiscal year.
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| Research Progress Status |
28年度が最終年度であるため、記入しない。
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| Strategy for Future Research Activity |
28年度が最終年度であるため、記入しない。
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