過飽和の解消による蛋白質の異常凝集体の形成に関する研究
| Project/Area Number |
14J04433
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| Research Category |
Grant-in-Aid for JSPS Fellows
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| Allocation Type | Single-year Grants |
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| Section | 国内 |
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| Research Field |
Biophysics
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| Research Institution | Osaka University |
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Principal Investigator |
林 雨曦 大阪大学, 理学研究科, 特別研究員(DC1)
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| Project Period (FY) |
2014-04-25 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 2016: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2015: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2014: ¥900,000 (Direct Cost: ¥900,000)
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| Keywords | protein aggregation / supersaturation / cytochrome c / solubility / phase diagram |
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| Outline of Annual Research Achievements |
I extended my study on protein aggregation from globular proteins to an intrinsically disordered protein, amyloid beta 1-40, this year. Misfolding and aggregation of amyloid beta 1-40 has been related to the onset of Alzheimer’s disease. Although much has been done to explore amyloid beta 1-40 aggregation, its detail mechanism is only partially understood.
I investigated the microscopic mechanism and pathway of amyloid beta 1-40 aggregation with macroscopic viewpoints using alcohols with the application of ultrasonication. The results suggested that the microscopic aggregation pathways of amyloid beta 1-40 depended on concentration and type of alcohols. Moreover, the addition of salts induced conversion of amyloid beta 1-40 from off-pathway oligomers into in-pathways protofibrils. Based on experimental results, I constructed phase diagrams of amyloid beta 1-40 aggregation in alcohols, which indicated that the concept of solubility and supersaturation also provided a macroscopic understanding of amyloid beta 1-40 aggregation.
In conclusion, these results gave a further insight into the aggregation process of amyloid beta 1-40, which is beneficial for developing small molecules to control amyloid beta 1-40 aggregation.
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| Research Progress Status |
28年度が最終年度であるため、記入しない。
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| Strategy for Future Research Activity |
28年度が最終年度であるため、記入しない。
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Report
(3 results)
Research Products
(9 results)
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[Journal Article] Energetic basis on interactions between ferredoxin and ferredoxin NADP+ reductase at varying physiological conditions.2016
Author(s)
Kinoshita, M., Kim, J.Y., S., Lin, Y., Hun Mok K., Kataoka, Y., Ishimori, K., Markova, N., Kurisu, G., Hase, T., Lee, Y.H.
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Journal Title
Biochem. Biophys. Res. Commun.
Volume: 482
Issue: 4
Pages: 909-915
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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