環境アレルゲンのオンサイトモニタリングを目的とした蛍光免疫計測システム

• Project/Area Number: 14J06141
• Research Category: Grant-in-Aid for JSPS Fellows
• Allocation Type: Single-year Grants
• Section: 国内
• Research Field: Environmental dynamic analysis
• Research Institution: Tokyo Institute of Technology
• Principal Investigator: 宮島 久美子 東京工業大学, 物質理工学院, 特別研究員(PD)
• Project Period (FY): 2014-04-25 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: アレルゲン / 免疫計測 / 磁性カプセル / FePt / 金ナノ粒子 / 光ファイバ

Outline of Annual Research Achievements

住環境にはアレルギー疾患を引き起こすアレルゲンが多種存在し、ハウスダストとともに堆積しているのみならず、居住者の日常生活動作に伴う気流の変化により空間中に浮遊・拡散している。アレルギー疾患の予防や治療のため、住環境における浮遊アレルゲンの濃度分布をモニタリングするには、気相成分の捕集から抗原の検出までを一括してオンサイトで実現することが求められる。そこで、抗原に特異的な抗体を磁気微粒子に固定化し、連続的に免疫学的測定を行うシステムを着想した。昨年度までに中空構造を有するマイクロメートルサイズの磁気微粒子を作製した。今年度は、作製した磁気微粒子表面への捕捉抗体固定化方法について検討をすすめ、Der f1に対する免疫計測への展開可能性を評価した。捕捉抗体の固定化方法として、磁気微粒子表面に金ナノ粒子（AuNP）を結合させる方法を採用した。まず、磁気微粒子にpoly(ethyleneimine) (PEI)を被覆し、その後tetrachloroauric(III) acid及び還元剤としてホルムアルデヒドを加えることによりAuNPを成長させた。水で洗浄することで成長反応を停止し、この磁気粒子と抗マウスIgG抗体を混合することで抗体を固定化した。磁気粒子と混合前後のIgG溶液について、液中のIgG量を比較したところ、粒子混合後にはIgGの顕著な減少が確認されたことから、粒子へのIgGの結合が示唆された。更に、得られた磁気微粒子をDer f1免疫計測に展開したところ、Der f1に由来するシグナル増強が確認されたことから、本粒子が連続的な免疫計測にも有効であることが示唆された。しかしながら、作製した捕捉抗体固定化粒子では抗原や検出抗体の非特異的な結合も観察されており、洗浄操作の改良等、今後更に検討が必要である。

Research Progress Status

28年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

28年度が最終年度であるため、記入しない。

Research Products

• [Journal Article] Fiber-optic chemifluorescent immunosensor for influenza virus monitoring (2016)
  • Author(s): Toma K, Suzuki Y, Saito M, Miyajima K, Arakawa T, Shimomura H, Mitsubayashi K
  • Journal Title: Journal of Analytical Bio-Science Volume: 39 Pages: 271-276
  • Peer Reviewed
• [Journal Article] Direct Measurement of Gaseous Formaldehyde from Food with a Fiber-Optic Biochemical Gas Sensor (Bio-Sniffer) (2016)
  • Author(s): Toma K, Miyajima K, Sawada S, Arakawa T, Kudo H, Akiyoshi K, Mitsubayashi K
  • Journal Title: Sensors and Materials Volume: 28 Pages: 1
  • DOI: 10.18494/SAM.2016.1288
  • ISSN: 0914-4935, 2435-0869
  • Peer Reviewed / Open Access
• [Journal Article] Chemifluorescent Optical Fiber Immunosensor for On-Site Bioaerosol Monitoring System (2015)
  • Author(s): K. Toma, Y. Suzuki, C. Kishikawa, M. Saito, K. Miyajima, T. Arakawa, H. Shimomura, K. Mitsubayashi
  • Journal Title: Sensors and Materials Volume: 27(11) Pages: 1113-1122
  • DOI: 10.18494/SAM.2015.1148
  • ISSN: 0914-4935, 2435-0869
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] A Fluorometric Biochemical Gas Sensor (Biosniffer) for Acetaldehyde Vapor Based on Catalytic Reaction of Aldehyde Dehydrogenase (2015)
  • Author(s): Y. Suzuki, M. Ye, K. Miyajima, T. Arakawa, S. Sawada, H. Kudo, K. Akiyoshi, K. Mitsubayashi
  • Journal Title: Sensors and Materials Volume: 27(11) Pages: 1123-1130
  • DOI: 10.18494/SAM.2015.1149
  • ISSN: 0914-4935, 2435-0869
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Fluoroimmunoassay System for Fiber-Optic Measurement of House Dust Mite Allergen (Der f1) (2015)
  • Author(s): K. Miyajima, H. Kon, T. Arakawa, K. Shiba, K. Mitsubayashi
  • Journal Title: Sensors and Materials Volume: 27(9) Pages: 871-880
  • DOI: 10.18494/SAM.2015.1123
  • ISSN: 0914-4935, 2435-0869
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Repetitive immunoassay with a surface acoustic wave device and a highly stable protein monolayer for on-site monitoring of airborne dust mite allergens (2015)
  • Author(s): K. Toma, D. Miki, C. Kishikawa, N. Yoshimura, K. Miyajima, T. Arakawa, H. Yatsuda, K. Mitsubayashi
  • Journal Title: Analytical Chemistry Volume: 87 (20) Issue: 20 Pages: 10470-10474
  • DOI: 10.1021/acs.analchem.5b02594
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Chemiluminescent imaging of transpired ethanol from the palm for evaluation of alcohol metabolism (2015)
  • Author(s): Arakawa T, Kita K, Wang X, Miyajima K, Toma K, Mitsubayashi K
  • Journal Title: Biosensors and Bioelectronics Volume: 67 Pages: 570-575
  • DOI: 10.1016/j.bios.2014.09.045
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Odourless Watermark (Digital Chemocode) System with Biochemical Sniff Scanner (2014)
  • Author(s): Saito H, Goto T, Miyajima K, Munkhjargal M, Arakawa T, Mitsubayashi K
  • Journal Title: Sensors and Materials Volume: 26 Pages: 109-119
  • Peer Reviewed / Open Access
• [Journal Article] Bioelectronic Sniffer (Biosniffer) Based on Enzyme Inhibition of Butyrylcholinesterase for Toluene Detection (2014)
  • Author(s): Saito H, Suzuki Y, Gessei T, Miyajima K, Arakawa T, Mitsubayashi K
  • Journal Title: Sensors and Materials Volume: 26 Pages: 121-129
  • Peer Reviewed / Open Access
• [Journal Article] Direct analysis of airborne mite allergen (Der f1) in the residential atmosphere by chemifluorescent immunoassay using bioaerosol sampler (2014)
  • Author(s): Miyajima K, Suzuki Y, Miki D, Arai M, Arakawa T, Shimomura H, Shiba K, Mitsubayashi K
  • Journal Title: Talanta Volume: 123 Pages: 241-246
  • DOI: 10.1016/j.talanta.2013.11.033
  • Peer Reviewed / Open Access
• [Presentation] SAW Immunosensor for On-site Monitoring of Airborne Mite Allergen Der f 1 in Residential Environment (2016)
  • Author(s): Kishikawa C, Miki D, Miyajima K, Yoshimura N, Yatsuda H, Toma K, Arakawa T, Mitsubayashi K
  • Organizer: International Symposium on Biomedical Engineering
  • Place of Presentation: Tokyo, Japan
  • Year and Date: 2016-11-10
  • Int'l Joint Research
• [Presentation] A regenerable SAW ( Surface Acoustic Wave) immunosensor for repetitive measurement of dust mite allergen (2016)
  • Author(s): Toma K, Miki D, Yoshimura N, Miyajima K, Arakawa T, Mitsubayashi K
  • Organizer: IMCS2016
  • Place of Presentation: Jeju, Korea
  • Year and Date: 2016-07-10
  • Int'l Joint Research
• [Presentation] A surface acoustic wave (SAW) immunosensor with a regeneratable surface enabling repetitive measurement of dust mite allergens (2016)
  • Author(s): Toma K, Miki D, Kishikawa C, Yoshimura N, Miyajima K, Arakawa T, Yatsuda H, Mitsubayashi K
  • Organizer: Biosensors 2016
  • Place of Presentation: Gothenburg, Sweden
  • Year and Date: 2016-05-25
  • Int'l Joint Research
• [Presentation] A chemi-fluorescence immunosensor with disposable PMMA fiber for detection of influenza viruses (2016)
  • Author(s): K. Toma, Y. Suzuki, M. Saito, K. Miyajima, T. Arakawa, H. Shimomura, K. Mitsubayashi
  • Organizer: EUROPT(R)ODE XIII
  • Place of Presentation: Graz, Austria
  • Year and Date: 2016-03-20
  • Int'l Joint Research
• [Presentation] 化学蛍光法によるインフルエンザウイルスの光ファイバ型免疫測定に関する研究 (2016)
  • Author(s): 岸川知里，鈴木友梨香，齋藤真奈，宮島久美子， 當麻浩司，荒川貴博，下村弘冶，三林浩二
  • Organizer: 第63回応用物理学会春季学術講演会
  • Place of Presentation: 東京
  • Year and Date: 2016-03-19
• [Presentation] Repetitive immunosensing with a highly stable protein monolayer and surface acoustic wave device for allergen monitoring (2015)
  • Author(s): K. Toma, D. Miki, N. Yoshimura, K. Miyajima, T. Arakawa, H. Yatsuda, K. Mitsubayashi
  • Organizer: Pacifichem 2015
  • Place of Presentation: Honolulu, Hawaii, USA
  • Year and Date: 2015-12-15
  • Int'l Joint Research
• [Presentation] A surface acoustic wave biosensor allowing repetitive immunoassay for dust mite allergen monitoring (2015)
  • Author(s): K. Toma, D. Miki, C. Kishikawa, N. Yoshimura, K. Miyajima, T. Arakawa, H. Yatsuda, K. Mitsubayashi
  • Organizer: Bio4Apps 2015
  • Place of Presentation: Fukuoka, Japan
  • Year and Date: 2015-12-09
  • Int'l Joint Research
• [Presentation] ダニアレルゲンDer f 1の半連続on-site計測のための表面弾性波(SAW)免疫センサに関する研究 (2015)
  • Author(s): 岸川知里，三木大輔，宮島久美子，吉村直之，當麻浩司，荒川貴博，谷津田博美，三林浩二
  • Organizer: 第27回SAS Symposium
  • Place of Presentation: 神奈川
  • Year and Date: 2015-11-12
• [Presentation] ダニアレルゲン免疫測定におけるSAWデバイスの新たな取り組み（半連続計測） (2015)
  • Author(s): 當麻浩司，三木大輔，鈴木友梨香，岸川知里，宮島久美子，吉村直之，荒川貴博，谷津田博美，三林浩二
  • Organizer: 弾性波素子技術第150委員会第142回研究会
  • Place of Presentation: 東京
  • Year and Date: 2015-10-10
  • Invited
• [Presentation] 環境アレルゲンモニタリング用光ファイバ式免疫計測システムのための磁性マイクロカプセルの作製 (2015)
  • Author(s): 宮島久美子，三輪勇樹，渕上輝顕，北本仁孝
  • Organizer: 2015年電気化学会秋季大会
  • Place of Presentation: 埼玉
  • Year and Date: 2015-09-11
• [Presentation] Repetitive immunoassay with a regeneration resistant protein and surface acoustic wave device for allergen monitoring (2015)
  • Author(s): K. Toma, D. Miki, N. Yoshimura, K. Miyajima, T. Arakawa, H. Yatsuda, K. Mitsubayashi
  • Organizer: EuroAnalysis 2015
  • Place of Presentation: Bordeaux, France
  • Year and Date: 2015-09-06
  • Int'l Joint Research
• [Presentation] Fabrication of porous fept micro-capsules for fiber-optic fluoroimmunoassay of environmental allergens (2015)
  • Author(s): K. Miyajima, Y. Miwa, T. Fuchigami, Y. Kitamoto
  • Organizer: th International Symposium on Organic and Inorganic Electronic Materials and Related Nanotechnologies (EM-NANO 2015)
  • Place of Presentation: Niigata , Japan
  • Year and Date: 2015-06-16
  • Int'l Joint Research
• [Presentation] Repetitive measurement of house dust mites (Der f1) with surface acoustic wave (SAW) immunosensor for on-site monitoring of indoor allergens (2015)
  • Author(s): K. Toma, D. Miki, N. Yoshimura, K. Miyajima, T. Arakawa, H. Yatsuda, K. Mitsubayashi
  • Organizer: 4th International Conference on Bio-Sensing Technology
  • Place of Presentation: Lisbon, Portugal
  • Year and Date: 2015-05-10
  • Int'l Joint Research
• [Presentation] Fiber-optic immunosensor for detection of airborne influenza viruses, 4th International Conference on Bio-Sensing Technology (2015)
  • Author(s): K. Toma, Y. Suzuki, M. Saito, K. Miyajima, T. Arakawa, H. Shimomura, K. Mitsubayashi
  • Organizer: 4th International Conference on Bio-Sensing Technology
  • Place of Presentation: Lisbon, Portugal
  • Year and Date: 2015-05-10
  • Int'l Joint Research
• [Presentation] 住環境中ダニアレルゲンのon-site計測のためのSAW免疫センサ (2015)
  • Author(s): 三木大輔, 吉村直之, 當麻浩司, 宮島久美子, 荒川貴博, 谷津田博美, 三林浩二
  • Organizer: 第62回応用物理学会春季学術講演会
  • Place of Presentation: 東海大学 湘南キャンパス、平塚市、神奈川
  • Year and Date: 2015-03-11 – 2015-03-14
• [Presentation] インフルエンザウイルスのための化学蛍光法を用いた光ファイバ型免疫測定に関する研究 (2015)
  • Author(s): 鈴木友梨香, 齋藤真奈, 下村弘治, 宮島久美子, 當麻浩司, 荒川貴博, 三林浩二
  • Organizer: 第25回生物試料分析科学会 年次学術集会
  • Place of Presentation: 文京学院大学 本郷キャンパス、文京区、東京
  • Year and Date: 2015-02-14 – 2015-02-15
• [Presentation] Fiber-optic Immunosensing device for house dust mite allergen in air (2014)
  • Author(s): Miyajima K, Arai M, Toma K, Arakawa T, Shimomura H, Mitsubayashi K
  • Organizer: International Conference on Bioelectronics, Biosensors, Biomedical Devices BioMEMS/NEMS, Applications 2014
  • Place of Presentation: Shanghai Jiao Tong University, Shanghai, China
  • Year and Date: 2014-11-17 – 2014-11-19
• [Presentation] Highly sensitive fiber-optic formaldehyde gas sensor (biosniffer) for food-sample detection (2014)
  • Author(s): Jen Chien P, Miyajima K, Munkhjargal M, Toma K, Arakawa T, Kudo H, Mitsubayashi K
  • Organizer: International Conference on Bioelectronics, Biosensors, Biomedical Devices BioMEMS/NEMS, Applications 2014
  • Place of Presentation: Shanghai Jiao Tong University, Shanghai, China
  • Year and Date: 2014-11-17 – 2014-11-19
• [Presentation] Gaseous ethanol imaging systemfrom human breath and skin for evaluation of alcohol metabolism (2014)
  • Author(s): Arakawa T, Kita K, Wang X, Miyajima K, Toma K, Mitsubayashi K
  • Organizer: International Conference on Bioelectronics, Biosensors, Biomedical Devices BioMEMS/NEMS, Applications 2014
  • Place of Presentation: Shanghai Jiao Tong University, Shanghai, China
  • Year and Date: 2014-11-17 – 2014-11-19
• [Presentation] 免疫化学蛍光法による浮遊ダニアレルゲンDer f1の計測 (2014)
  • Author(s): 栗原康司, 鈴木友梨香, 宮島久美子, 新井萌花, 下村弘治, 荒川貴博, 當麻浩司, 三林浩二
  • Organizer: ’14 SAS インテリジェントシンポジウム
  • Place of Presentation: 東海大学 湘南キャンパス、平塚市、神奈川
  • Year and Date: 2014-11-13 – 2014-11-14
• [Presentation] 多孔質中空粒子の網目状構造シェル形成に与えるシリカコートの影響 (2014)
  • Author(s): 三輪勇樹，渕上輝顕，宮島久美子，北本仁孝
  • Organizer: 粉体粉末冶金協会平成26年度秋季大会（第11４回講演大会）
  • Place of Presentation: 大阪大学コンベンションセンター、吹田市、大阪
  • Year and Date: 2014-10-29 – 2014-10-30
• [Presentation] 住環境アレルゲン動態評価のための光ファイバ式蛍光免疫計測システム (2014)
  • Author(s): 三木大輔, 宮島久美子, 當麻浩司, 荒川貴博, 三林浩二
  • Organizer: 第四回次世代センサ研究発表会
  • Place of Presentation: 東京ビッグサイト、江東区、東京
  • Year and Date: 2014-09-17
• [Presentation] Fiber-optic chemifluorescent immunoassay system for measurement of mite allergen Der f1 (2014)
  • Author(s): Suzuki Y, Miyajima K, Arai M, Arakawa T, Shimomura H, Shiba K, Mitsubayashi K
  • Organizer: International Union of Materials Research Societies- The IUMRS International Conference in Asia 2014
  • Place of Presentation: Fukuoka University, Fukuoka-City, Fukuoka
  • Year and Date: 2014-08-24 – 2014-08-30
• [Presentation] 2-dimensional gaseous ethanol visualization system for noninvasive analysis of alcohol metabolism from exhaled human breath (2014)
  • Author(s): Jen Chien P, Ye M, Miyajima K, Arakawa T, Mitsubayashi K
  • Organizer: International Union of Materials Research Societies- The IUMRS International Conference in Asia 2014
  • Place of Presentation: Fukuoka University, Fukuoka-City, Fukuoka
  • Year and Date: 2014-08-24 – 2014-08-30
• [Presentation] Biocatalysis-based chemo-mechanical actuator for autonomous drug release system for blood sugar control in Diabetes (2014)
  • Author(s): Munkhjargal M, Sato R, Takagi H, Miyajima K, Arakawa T, Mitsubayashi K
  • Organizer: International Union of Materials Research Societies- The IUMRS International Conference in Asia 2014
  • Place of Presentation: Fukuoka University, Fukuoka-City, Fukuoka
  • Year and Date: 2014-08-24 – 2014-08-30
• [Presentation] Breath alcohol imaging with enzyme mesh after drinking (2014)
  • Author(s): Sato T, Wang X, Miyajima K, Toma K, Munkhjargal M, Arakawa T, Mitsubayashi K
  • Organizer: Breath Analysis 2014
  • Place of Presentation: Filmar Hotel, Torun, Poland
  • Year and Date: 2014-07-06 – 2014-07-09
• [Presentation] Fiber-optic chemifluorescent immunoassay for continuous monitoring of airborne mite allergen Der f1 (2014)
  • Author(s): Miyajima K, Suzuki Y, Miki D, Arai M, Toma K, Arakawa T, Shimomura H, Mitsubayashi K
  • Organizer: 38th International Symposium on Environmental Analytical Chemistry
  • Place of Presentation: SwissTech Convention Center (Ecole Polytechnique Federale de Lausanne), Lausanne/Switzerland
  • Year and Date: 2014-06-17 – 2014-06-20
• [Presentation] Bioaerosol analysis of house dust mite allergen (Der f1) by fiber-optic chemifluorescent immunoassay system (2014)
  • Author(s): Miyajima K, Suzuki Y, Miki D, Arai M, Arakawa T, Shimomura H, Shiba K, Mitsubayashi K
  • Organizer: Biosensors 2014
  • Place of Presentation: Melbourne Convention and Exhibition Centre, Melbourne, Australia
  • Year and Date: 2014-05-27 – 2014-05-30
• [Presentation] Direct imaging system of gaseous ethanol from exhaled human breath for non-invasive analysis of alcohol metabolism (2014)
  • Author(s): Arakawa T, Wang X, Kajiro T, Miyajima K, Yano K, Mitsubayashi K
  • Organizer: Biosensors 2014
  • Place of Presentation: Melbourne Convention and Exhibition Centre, Melbourne, Australia
  • Year and Date: 2014-05-27 – 2014-05-30
• [Book] クリーンテクノロジー2015年1月号「医療・健康科学のためのバイオセンシング 気相バイオ計測技術を用いた生体ガス及びアレルゲンモニタリング」 (2015)
  • Author(s): 宮島久美子，三林浩二
  • Total Pages: 80
  • Publisher: 日本工業出版株式会社