SYSTEMATIC COLLECTION OF BASIS INFORMATION FOR PROTEIN INTERACTION PREDICTION

• Project/Area Number: 15014213
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: THE UNIVERSITY OF TOKYO
• Principal Investigator: ITO Takashi THE UNIVERSITY OF TOKYO, GRADUATE SCHOOL OF FRONTIER SCIENCES, PROFESSOR, 大学院新領域創成科学研究科, 教授 (90201326)
• Co-Investigator(Kenkyū-buntansha): SUMMOTO Hidekl KYUSHUUNIVERSITY, MEDICAL INSTITUTE OF BIO-REGULATION, PROFESSOR, 生体防御医学研究所, 教授 (30179303) ; OTA Kazuhisa THE UNIVERSITY OF TOKYO, GRADUATE SCHOOL OF FRONTIER SCIENCES, RESEARCH ASSOCIATE, 大学院新領域創成科学研究科, 助手 (00322727) ; YAMADA Yoichi KANAZAWA UNIVERSITY, GRADUATE SCHOOL OF NATURAL SCIENCES, RESEARCH ASSOCIATE, 大学院自然科学研究科, 助手 (30377402)
• Project Period (FY): 2003 – 2004
• Project Status: Completed (Fiscal Year 2004)
• Budget Amount: ¥50,600,000 (Direct Cost: ¥50,600,000); Fiscal Year 2004: ¥25,600,000 (Direct Cost: ¥25,600,000); Fiscal Year 2003: ¥25,000,000 (Direct Cost: ¥25,000,000)
• Keywords: PROTEIN-PROTEIN INTERACTION / YEAST 2-HYBRID SYSTEM / PB1 DOMAIN / TAP TAG / UBIQUITIN / MASS SPECTROMETRY / DNA METHYLATION / FULL LENGTH cDNA / 蛋白質間相互作用 / 2ハイブリッド法 / 質量分析法 / ユビキチン化 / 蛋白質DNA相互作用 / 1ハイブリッド法 / 相互作用ドメイン / 蛋白質核酸相互作用 / 転写開始点 / 出芽酵母

Research Abstract

1)We performed a comprehensive two-hybrid analysis for the budding yeast proteome and made the data available.
2)We utilized the two-hybrid clone resource to conduct genome-wide screens for chromation bariier proteins as well as transcriptional activators.
3)We developed two-hybrid footprinting, dual-bait reverse two-hybrid method, and accelerated phylogenetic footprinting to conduct interaction-targeting or targeted disruption of proteinprotein interactions.
4)We conducted a search for rules for protein-protein interactions by means of data-mining using the association rule discovery.
5)We compared yeast and mammalian systems of mutually interacting proteins including PB1-domain proteins and revealed two-different modes of target recognition by PB1 domains, enabling us to predict the PB1 interactions.
6)We developed a novel TAP (Tandern Affinity Purification) tag that am be used with crosslinking agents for efficient interaction profiling.
7)We developed a method of PAP (Parallel Affinity Purification) to enrich ubiquitinated proteins for degradome profiling.
8)We developed a methylation-dependent one-hybrid system to examine interactions between methylated DNA and proteins.
9)We developed HM-PCR for allelic methylation analysis and applied it to a comprehensive analysis of CpG islands on human chromosome 21.
10)We developed a method for chimaerization-mediated activation of transcription factors and applied it to decipher a gene network regulating multidrug resistance of the budding yeast
11)We systematically analyzed budding yeast full-length cDNA clones to reveal transcription starting sites for-4,000 genes.

Research Products

• [Journal Article] Transcriptional activators in yeast (2006)
  • Author(s): Titz, B., Seesandra, T., Rajagopala, S.V., Chiba, T., Ito, T., Uetz, P.
  • Journal Title: Nucleic Acids Res. 34 Pages: 955-967
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Comparative genomics approach toward critical determinants for the imprinting of an evolutionarily conserved gene Impact (2005)
  • Author(s): Okamura, K., Sakaki, Y., Ito, T.
  • Journal Title: Biochem. Biophys. Res. Commun. 329 Pages: 824-830
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] The yeast eIF4E-associated protein Eaplp attenuates GCN4 translation upon TOR inactivation (2005)
  • Author(s): Matsuo, R. et al.
  • Journal Title: FEBS Lett. 579 Pages: 2433-2438
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] A novel strategy to design highly specific PCR primers based on the stability and uniqueness of 3'-end subsequences (2005)
  • Author(s): Miura, F., Uematsu, C., Sakaki, Y., Ito, T.
  • Journal Title: Bioinformatics 21 Pages: 4363-4370
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Comparative genomics approach toward critical determinants for the imprinting of an evolutionarily conserved gene Impact (2005)
  • Author(s): Okamura, K., Sakaki, Y., Ito, T.
  • Journal Title: Biochem.Biophys.Res.Commun. 329 Pages: 824-830
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] The yeast eIF4E-associated protein Eap 1p attenuates GCN4 translation upon TOR inactivation (2005)
  • Author(s): Matsuo, R., Kubota, H., Obata, T., Kito, K., Ota, K., Kitazono, T., Ibayashi, S., Sasaki, T., Iida, M., Ito, T.
  • Journal Title: FEBS Lett. 579 Pages: 2433-2438
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] A novel strategy to design highly specific PCR primers based on the stability and uniqueness of 3'-end subsequences (2005)
  • Author(s): Miura, R., Uematsu, C., Sakaki, Y., Ito, T.
  • Journal Title: Bioinformatics 21 Pages: 4363-4370
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] A yeast one-hybrid system to detect methylation-dependent DNA-protein interactions (2004)
  • Author(s): Feng, S.-Y., Ota, K., Yamada, Y., Sawabu, N., Ito, T.
  • Journal Title: Biochem. Biophys. Res. Commun. 313 Pages: 922-925
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] A comprehensive analysis of allelic methylation status of CpG islands on human chromosome 21q (2004)
  • Author(s): Yamada, Y. et al.
  • Journal Title: Genome Res. 14 Pages: 247-266
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Barrier proteins remodel and modify chromatin to restrict silenced domains (2004)
  • Author(s): Oki, M., Valenzuera, L., Chiba, T., Ito, T., Kamakaka, R.
  • Journal Title: Mol. Cell. Biol. 24 Pages: 1956-1967
  • NAID: 120000808012
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Analysis of gene network regulating yeast multidrug resistance by artificial activation of transcription factors : involvement of Pdr3 in salt resistance (2004)
  • Author(s): Dnda, M., Ota, K., Chiba, T., Sakaki, Y., Ito.T.
  • Journal Title: Gene 332 Pages: 51-59
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Transcriptomic and proteomic analysis of a 14-3-3 gene-deficient yeast (2004)
  • Author(s): Ichimura, T. et al.
  • Journal Title: Biochemistry 43 Pages: 6149-6158
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Mass spectrometry-based proteomics for quantitative description of cellular events (2004)
  • Author(s): Kito, K., Ito, T.
  • Journal Title: Curr. Genomics 5 Pages: 629-635
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] An evolutionary scenario for genomic imprinting of Impact lying between nonimprinted neighbors. (2004)
  • Author(s): Okamura, K., Yamada, Y., Sakaki, Y., Ito, T.
  • Journal Title: DNA Res. 11 Pages: 381-390
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] A yeast one-hybrid system to detect methylation-dependent DNA-protein interactions (2004)
  • Author(s): Feng, S.-Y., Ota, K., Yamada, Y., Sawabu, N., Ito, T.
  • Journal Title: Biochem.Biophys.Res.Commun 313 Pages: 922-925
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] A comprehensive analysis of allelic methylation status of CpG islands on human chromosome 21q (2004)
  • Author(s): Yamada, Y., Watanabe, H., Miura, F., Soejima, H., Uchiyama, M., Iwasaka, T., Mukai, T., Sakaki, Y., Ito, T.
  • Journal Title: Genome Res. 14 Pages: 247-266
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Barrier proteins remodel and modify chromatin to restrict silenced domains (2004)
  • Author(s): Oki, M., Valenzuera, L., Chiba, T., Ito, T., Kamakaka, R.
  • Journal Title: Mol.Cell.Biol. 24 Pages: 1956-1967
  • NAID: 120000808012
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Analysis of gene network regulating yeast multidrug resistance by artificial activation of transcription factors : involvement of Pdr3 in salt resistance (2004)
  • Author(s): Onda, M., Ota, K., Chiba, T., Sakaki, Y., Ito, T.
  • Journal Title: Gene 332 Pages: 51-59
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Transcriptomic and proteomic analysis of a 14-3-3 gene-deficient yeast (2004)
  • Author(s): Ichimura, T., Kubota, H., Goma, T., Mizushima, N., Ohsumi, Y., Iwago, M., Kakiuchi, K., Shekhar, H.U., Shinkawa, T., Taoka, M., Ito, T., Isobe, T.
  • Journal Title: Biochemistry 43 Pages: 6149-6158
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Mass spectrometry-based proteomics for quantitative description of cellular events (2004)
  • Author(s): Kito, K., Ito, T.
  • Journal Title: Curr.Genomics 5 Pages: 629-635
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] An evolutionary scenario for genomic imprinting of Impact lying between nonimprinted neighbors (2004)
  • Author(s): Okamura, K., Yamada, Y., Sakaki, Y., Ito, T.
  • Journal Title: DNA Res. 11 Pages: 381-390
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] A yeast one-hybrid system to detect methylation-dependent DNA-protein interactions. (2004)
  • Author(s): Feng, S.-Y.et al.
  • Journal Title: Biochem.Biophys.Res.Commun. 313 Pages: 922-925
• [Journal Article] A comprehensive analysis of allelic methylation status of CpG islands on human chromosome 21q. (2004)
  • Author(s): Yamada, Y. et al.
  • Journal Title: Genome Res. 14 Pages: 247-266
• [Journal Article] Barrier proteins remodel and modify chromatin to restrict silenced domains. (2004)
  • Author(s): Oki, M.et al.
  • Journal Title: Mol.Cell.Biol. 24 Pages: 1956-1967
• [Journal Article] Analysis of gene network regulating yeast multidrug resistance by artificial activation of transcription factors : involvement of Pdr3 in salt resistance. (2004)
  • Author(s): Onda, M. et al.
  • Journal Title: Gene 332 Pages: 51-59
• [Journal Article] Transcriptomic and proteomic analysis of a 14-3-3 gene-deficient yeast. (2004)
  • Author(s): Ichimura, T. et al.
  • Journal Title: Biochemistry 43 Pages: 6149-6158
• [Journal Article] Mass spectrometry-based proteomics for quantitative description of cellular events. (2004)
  • Author(s): Kito, K., Ito, T.
  • Journal Title: Curr.Genomics 5 Pages: 629-635
• [Journal Article] Adaptor protein p40^<phox> as a positive regulator of the superoxide-producing phagocyte oxidase (2003)
  • Author(s): Kuribayashi, F. et al.
  • Journal Title: EMBO J. 21 Pages: 6312-6320
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Rapamycin-induced translational derepression of GCN4 mRNA involves a novel mechanism for activation of the eIF2α kinase GCN2 (2003)
  • Author(s): Kubota, H., Obata, T., Ota, K., Sasaki, T., Ito, T.
  • Journal Title: J. Biol. Chem. 278 Pages: 20457-20460
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Phosphorylation of p47^<phox> directs phox homology domain from SH3 domain toward phosphoinositide, leading to phagocyte NADPH oxidase activation (2003)
  • Author(s): Ago, T.et al.
  • Journal Title: Proc. Natl. Acad. Sci. USA 100 Pages: 4474-4479
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] The PB1 domain and the PC motif-containing region are structurally similar protein binding modules (2003)
  • Author(s): Yoshinaga, S. et al.
  • Journal Title: EMBO J. 22 Pages: 4888-4897
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Molecular recognition in dimerization between PB1 domains (2003)
  • Author(s): Noda, Y. et al.
  • Journal Title: J. Biol. Chem. 278 Pages: 43516-43524
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Identification of a five-pass transmembrane protein family localizing in the Golgi apparatus and the ER (2003)
  • Author(s): Shakoori, A. et al.
  • Journal Title: Biochem. Biophys. Res. Commun. 312 Pages: 850-857
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Adaptor protein p4O^<phox> as a positive regulator of the superoxide-producing phagocyte oxidase (2003)
  • Author(s): Kuribayashi, F., Nunoi, H., Wakamatsu, K., Tsunawaki, S., Sato, K., Ito, T., Sumimoto, H.
  • Journal Title: EMBO J. 21 Pages: 6312-6320
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Rapamycin-induced translational derepression of GCN4 mRNA involves a novel mechanism for activation of the eIF2a kinase GCN2 (2003)
  • Author(s): Kubota, H., Obata, T., Ota, K., Sasaki, T., Ito, T.
  • Journal Title: J.Biol.Chem. 278 Pages: 20457-20460
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Phosphorylation of p47^<phox> directs phox homology domain from SH3 domain toward phosphoinositide, leading to phagocyte NADPH oxidase activation (2003)
  • Author(s): Ago, T., Kuribayashi, F., Hiroaki, H., Takeya, R., Ito, T., Kohda, D., Sumimoto, H.
  • Journal Title: Proc.Natl.Acad.Sci.USA 100 Pages: 4474-4479
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] The PB1 domain and the PC motif-containing region are structurally similar protein binding modules (2003)
  • Author(s): Yoshinaga, S., Kohjima, M., Ogura, K., Yokochi, M., Takeya, R., Ito, T., Sumimoto, H., Inagaki, F.
  • Journal Title: EMBO J. 22 Pages: 4888-4897
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Molecular recognition in dimerization between PB1 domains (2003)
  • Author(s): Noda, Y., Kohjima, M., Izaki, T., Ota, K., Yoshinaga, S., Inagaki, F., Ito, T., Sumimoto, H.
  • Journal Title: J.Biol.Chem. 278 Pages: 43516-43524
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Identification of a five-pass transmembrane protein family localizing in the Golgi apparatus and the ER (2003)
  • Author(s): Shakoori, A., Fujii, G., Yoshimura, S., Kitamura, M., Nakayama, K., Ito, T., Ohno, H., Nakamura, N.
  • Journal Title: Biochem.Biophys.Res.Commun 312 Pages: 850-857
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] OPR, PC and AID : all in the PB1 family (2002)
  • Author(s): Ponting, C.P., Ito, T.et al.
  • Journal Title: Trends Biochem. Sci. 27 Pages: 10-10
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Extraction of knowledge on protein-protein interaction by association rule discovery (2002)
  • Author(s): Oyama, T., Kitano, K., Satou, K., Ito, T.
  • Journal Title: Bioinformatics 18 Pages: 705-714
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Roles for the two-hybrid system in exploration of the yeast protein interactome (2002)
  • Author(s): Ito, T.et al.
  • Journal Title: Mol. Cell. Proteomics 1 Pages: 561-566
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] OPR, PC and AID : all in the PB1 family (2002)
  • Author(s): Ponting, C.P., Ito, T., Moscat, J., Diaz-Meco, M.T., Inagaki, F., Sumimoto, H.
  • Journal Title: Trends Biochem.Sci. 27 Pages: 10-10
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Roles for the two-hybrid system in exploration of the yeast protein interactome (2002)
  • Author(s): Ito, T., Ota, K., Kubota, H., Yamaguchi, Y., Chiba, T., Sakuraba, K., Yoshida, M.
  • Journal Title: Mol.Cell.Proteomics 1 Pages: 561-566
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] A comprehensive two-hybrid analysis to explore the yeast protein interactome (2001)
  • Author(s): Ito, T., Chiba, T., Ozawa, T., Yoshida, M., Hattori, M., Sakaki, Y.
  • Journal Title: Proc. Natl. Acad. Sci. USA 98 Pages: 4569-4574
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Budding yeast GCN1 binds the GI domain to activate the eIF2α kinase GCN2 (2001)
  • Author(s): Kubota, H., Ota, K., Sakaki, Y., Ito, T.
  • Journal Title: J. Biol. Chem. 276 Pages: 17591-17596
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Novel modular domain PB1 recognizes PC motif to mediate functional protein-protein interactions (2001)
  • Author(s): Ito, T., Matsui, Y., Ago, T., Ota, K., Sumimoto, H.
  • Journal Title: EMBO J. 20 Pages: 3938-3946
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Structure and ligand recognition of the PB1 domain : a novel protein module binding to the PC motif (2001)
  • Author(s): Terasawa, H., Noda, Y., Ito, T.et al.
  • Journal Title: EMBO J. 20 Pages: 3947-3956
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Solution structure of the PX domain, a target of the SH3 domain (2001)
  • Author(s): Hiroaki, H., Ago, T., Ito, T., Sumimoto, H., Kohda, D.
  • Journal Title: Nature Struct. Biol. 8 Pages: 526-530
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] The PX domain as a novel phosphoinositide-binding module (2001)
  • Author(s): Ago, T.et al.
  • Journal Title: Biochem. Biophys. Res. Commun. 287 Pages: 733-738
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Exploring the protein interactome using comprehensive two-hybrid projects (2001)
  • Author(s): Ito, T., Chiba, T., Yoshida, M.
  • Journal Title: Trends Biotechnol. 19
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Human homologues of the Caenorhabditis elegans cell polarity protein PAR6 as an adaptor that links the small GTPases Rac and Cdc42 to atypical protein kinase C (2001)
  • Author(s): Noda, Y., Takeya, R., Ohno, S., Naito, S., Ito, T., Sumimoto, H.
  • Journal Title: Genes Cells 6 Pages: 107-120
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] A comprehensive two-hybrid analysis to explore the yeast protein interactome (2001)
  • Author(s): Ito, T., Chiba, T., Ozawa, T., Yoshida, M., Hattori, M., Sakaki, Y.
  • Journal Title: Proc.Natl.Acad.Sci.USA 98 Pages: 4569-4574
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Budding yeast GCN1 binds the GI domain to activate the eIF2α kinase GCN2 (2001)
  • Author(s): Kubota, H., Ota, K., Sakaki, Y., Ito, T.
  • Journal Title: J.Biol.Chem. 276 Pages: 17591-17596
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Structure and ligand recognition of the PB1 domain : a novel protein module binding to the PC motif (2001)
  • Author(s): Terasawa, H., Noda, Y., Ito, T., Hatanaka, H., Ichikawa, S., Ogura, K., Sumimoto, H., Inagaki, F.
  • Journal Title: EMBO J. 20 Pages: 3947-3956
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Solution structure of the PX domain, a target of the SH3 domain (2001)
  • Author(s): Hiroaki, H., Ago, T., Ito, T., Sumimoto, H., Kohda, D.
  • Journal Title: Nature Struct.Biol. 8 Pages: 526-530
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] The PX domain as a novel phosphoinositide-binding module (2001)
  • Author(s): Ago, T., Takeya, R., Kuribayashi, F., Hiroaki, H., Ito, T., Kohda, D., Sumimoto, H.
  • Journal Title: Biochem.Biophys.Res.Commun. 287 Pages: 733-738
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Toward a protein-protein interaction map of the budding yeast : a comprehensive system to examine two-hybrid interactions in all possible combinations between the yeast proteins (2000)
  • Author(s): Ito, T.et al.
  • Journal Title: Proc. Natl. Acad. Sci. USA 97 Pages: 1143-1147
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] GI domain-mediated association of the eukaryotic initiation factor 2α kinase GCN2 with its activator GCN1 is required for general amino acid control in budding yeast (2000)
  • Author(s): Kubota, H., Sakaki, Y., Ito, T.
  • Journal Title: J. Biol. Chem. 275 Pages: 20243-20246
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Interaction of the PDZ domain of human PICK1 with class I ADP-ribosylation factors (2000)
  • Author(s): Takeya, R., Takeshige, K., Sumimoto, H.
  • Journal Title: Biochem. Biophys. Res. Commun. 267 Pages: 149-155
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Arachidonic acid and phosphorylation synergistically induce a conformational change of p47^<phox> to activate the phagocyte NADPH oxidase (2000)
  • Author(s): Shiose, A., Sumimoto, H.
  • Journal Title: J. Biol. Chem. 275 Pages: 13793-13801
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Human homologues of the Caenorhabditis elegans cell polarity protein PAR6 as an adaptor that links the small GTPases Rac and Cdc42 to atypical protein kinase C (2000)
  • Author(s): Noda, Y., Takeya, R., Ohno, S., Naito.S., Ito, T., Sumimoto, H.
  • Journal Title: Genes Cells 6 Pages: 106-120
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Toward a protein-protein interaction map of the budding yeast : a comprehensive system to examine two-hybrid interactions in all possible combinations between the yeast proteins (2000)
  • Author(s): Ito, T., Tashiro, K., Muta, S., Ozawa, R., Chiba, T., Nishizawa, M., Yamamoto, K., Kuhara, S., Sakaki, Y.
  • Journal Title: Proc.Natl.Acad.Sci.USA 97 Pages: 1143-1147
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] GI domain-mediated association of the eukaryotic initiation factor 2a kinase GCN2 with its activator GCN1 is required for general amino acid control in budding yeast (2000)
  • Author(s): Kubota, H., Sakaki, Y., Ito, T.
  • Journal Title: J.Biol.Chem. 275 Pages: 20243-20246
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Interaction of the PDZ domain of human PICK1 with class I ADP-ribosylation factors (2000)
  • Author(s): Takeya, R., Takeshige, K., Sumimoto, H.
  • Journal Title: Biochem.Biophys.Res.Commun. 267 Pages: 149-155
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Arachidonic acid and phosphorylation synergistically induce a conformational change of p47Ph0r to activate the phagocyte NADPH oxidase (2000)
  • Author(s): Shiose, A., Sumimoto, H.
  • Journal Title: J.Biol.Chem. 275 Pages: 13793-13801
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Ago, T.et al.: "Phosphorylation of p47^<phox> directs PX domain from SH3 domain toward phosphoinositides, leading to activation of the phagocyte NADPH oxidase."Proc.Natl.Acad.Sci.USA. 100. 4474-4479 (2003)
• [Publications] Kubota, H. et al.: "Rapamycin-induced translational derepression of GCN4 mRNA involves a novel mechanism for activation of the eIF2α kinase GCN2"J.Biol.Chem.. 278. 20457-20460 (2003)
• [Publications] Yoshinaga, S. et al.: "The PB1 domain and the PC motif-containing region are structurally similar protein binding modules"EMBO J.. 22. 4888-4897 (2003)
• [Publications] Noda, Y. et al.: "Molecular recognition in dimerization between PB1 domains"J.Biol.Chem.. 278. 43516-43524 (2003)
• [Publications] Feng, S.-Y.et al.: "A yeast one-hybrid system to detect methylation-dependent DNA-protein interactions"Biochem.Biophys.Res.Commun.. 313. 922-925 (2004)
• [Publications] Yamada, Y. et al.: "A comprehensive analysis of allelic methylation status of CpG islands on human chromosome 21q"Genome Res.. 14. 247-266 (2004)