哺乳動物未受精卵に含まれる初期化因子の探索

• Project/Area Number: 15039233
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Kinki University
• Principal Investigator: 加藤 容子 近畿大学, 農学部, 助教授 (40278742)
• Project Period (FY): 2003 – 2004
• Project Status: Completed (Fiscal Year 2004)
• Budget Amount: ¥4,100,000 (Direct Cost: ¥4,100,000); Fiscal Year 2004: ¥1,800,000 (Direct Cost: ¥1,800,000); Fiscal Year 2003: ¥2,300,000 (Direct Cost: ¥2,300,000)
• Keywords: 核移植 / 初期化 / クローン / 卵子 / 初期化因子 / 体細胞クローン

Research Abstract

前年度は,体細胞あるいはES細胞を除核未受精卵へ核移植後,胚盤胞への発生能を支持しなくなる卵細胞質を作り出す条件を検討した。今年度はその結果を参考に,発生を支持する能力を持つ卵細胞質と支持しない卵細胞質との間で2次元電気泳動等を行ない,両者間にタンパクレベルで差異があるかどうかを調べた。
方法:MII期未受精卵に電気刺激等による活性化刺激を付与した卵子を集め,活性化刺激を付与していない卵子との間で2次元電気泳動による比較をおこなった。
結果:両者の2次元電気泳動像を比較検討したところ,1カ所で著しい差異のあるスポットが見つかったので,スポットの差異に注目して詳細を検討した。その結果,それはヒトやマウス等で既知のタンパクであることが分かった。今後,そのタンパクの性質やそれが初期化に直接関与しているかをさらに詳細に検討する必要があると考えている。

Research Products

• [Journal Article] Effect of low-temperature bovine ovary storage on the maturation rate and developmental potential of follicular oocytes after in vitro fertilization, parthenogenetic activation, or somatic cell nucleus transfer (2004)
  • Author(s): Matsushita S., et al.
  • Journal Title: Animal Reproduction Science 84 Pages: 293-301
• [Journal Article] Effect of the timing of the first cleavage on the developmental potential of nuclear-transferred mouse oocytes receiving embryonic stem cells (2004)
  • Author(s): Kobayashi T., et al.
  • Journal Title: Theriogenology 62 Pages: 854-860
• [Journal Article] Comparison of in vitro development of porcine nuclear-transferred oocytes receiving fetal somatic cells by injection and fusion methods (2004)
  • Author(s): Kawano K., et al.
  • Journal Title: Cloning and Stem Cells 6 Pages: 67-72
• [Journal Article] Effects of nuclear transfer procedures on ES cell cloning efficiency in the mouse (2004)
  • Author(s): Yabuuchi A., et al.
  • Journal Title: J.Reproduction and Development 50 Pages: 263-268
• [Journal Article] Cryopreservation of bovine somatic cell nuclear-transferred blastocysts : effect of developmental stage (2004)
  • Author(s): Amarnath D., et al.
  • Journal Title: J.Reproduction and Development 50 Pages: 593-598
• [Publications] T.Tani, Y.Kato, Y.Tsunoda: "Reprogramming of bovine somatic cell nuclei is not directly regulated by maturation promoting factor or mitogen-activated protein kinase activity"Biology of Reproduction. 69. 1890-1894 (2003)
• [Publications] Kato, Imabayashi, Mori, Tani, Taniguchi, Higashi, Matsunoto, Umezawa, Tsunoda: "Nuclear transfer of adult bone marrow mesenchymal stem cells : developmental totipo tency"Biology of Reproduction. 70. 415-418 (2004)
• [Publications] A.Yabuuchi, Y.Yasuda, Y.Kato, Y.Tsunoda: "Effect of nuclear transfer procedures on ES cell cloning efficiency in the mouse"J.Reprod.Develop.. (In press). (2004)
• [Publications] T.Kobayashi, Y.Kato, Y.Tsunoda: "Effect of the timing of the first cleavage on the developmental potential of nuclear-transferred mouse oocytes receiving embryonic stem cells"Theriogenology. (In press). (2004)
• [Publications] K.Kawano, Y.Kato, Y.Tsunoda: "Comparison of in vitro development of porcine nuclear-transferred oocytes receiving fetal"Cloning and Stem Cells. (In press).
• [Publications] M.Kawakami, Tani, T, Yabuuchi, A, Kobayashi, T, Murakami, H, Fujimura, Y, Kato, Y.Tsun: "Effect of demecolcine and nocodazole on efficiency of chemically assisted removal of"Cloning and Stem Cells. 5. 379-387 (2003)