プロトンポンプの方向性を決定する内部結合水の構造解析

• Project/Area Number: 15076202
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Science and Engineering
• Research Institution: Nagoya Institute of Technology
• Principal Investigator: 神取 秀樹 Nagoya Institute of Technology, 工学研究科, 教授 (70202033)
• Co-Investigator(Kenkyū-buntansha): 古谷 祐詞 名古屋工業大学, 工学研究科, 助教 (80432285)
• Project Period (FY): 2003 – 2007
• Project Status: Completed (Fiscal Year 2007)
• Budget Amount: ¥83,500,000 (Direct Cost: ¥83,500,000); Fiscal Year 2007: ¥10,000,000 (Direct Cost: ¥10,000,000); Fiscal Year 2006: ¥10,000,000 (Direct Cost: ¥10,000,000); Fiscal Year 2005: ¥11,600,000 (Direct Cost: ¥11,600,000); Fiscal Year 2004: ¥17,000,000 (Direct Cost: ¥17,000,000); Fiscal Year 2003: ¥34,900,000 (Direct Cost: ¥34,900,000)
• Keywords: 生物物理 / ナノバイオ / 生体分子 / 蛋白質 / 光スイッチ / 内部結合水 / 赤外分光 / 水素結合 / プロトンポンプ / 赤外分光法 / スイッチ / バクテリオロドプシン / 塩素イオンポンプ / レチナール / 光受容蛋白質 / 機能 / 構造変化 / 水素結合ネットワーク / 同位体効果

Research Abstract

蛋白質は一般に疎水部を水から避ける形で折り畳まれるが、疎水的な内部に水分子が存在する場合もある。これら内部結合水の中には単に空間を充填するだけでなく、機能に重要な役割を演ずる水分子の存在が考えられている。本研究では、濃度勾配に逆らってイオンを輸送する分子ポンプ蛋白質の機能発現過程における内部結合水の役割を明らかにするため、光駆動プロトンポンプであるバクテリオロドプシンなどを対象として、低温赤外分光を用いた研究を行っている。本年度は以下の成果を得た。
バクテリオロドプシンの活性中心を構成する2つのアスパラギン酸(Asp85,212)を非解離性のアミノ酸に置換するとプロトンポンプ活性がなくなる。一方、クロライドを結合するとAsp85の変異体はクロライドポンプに機能転換する一方、Asp212の変異体はプロトンポンプ機能が復活することが知られているが、そのメカニズムは不明であった。我々は昨年、クロライドポンプに転換したD85変異体の構造解析を報告したが、それに続いてD212変異体の赤外分光解析を行った。その結果、シッフ塩基の水素結合アクセプターである水分子の位置にクロライドが結合するD85変異体とは異なり、D212変異体ではシッフ塩基-水-Asp85という水素結合ネットワークを再構成するようにクロライドが活性中心近傍に結合することが明らかになった。強い水素結合を形成した水分子は、ハライドが結合したD212変異体にのみ観測され、ロドプシンのプロトンポンプ機能とも完全に対応している。これ以外にも、バクテリオロドプシンのAsp115の変異体や海洋性バクテリアのプロトンポンプ蛋白質であるプロテオロドプシン(PR)に対して赤外分光解析を行い、特異的な水素結合構造が機能発現に重要な役割を担っていることを明らかにした。
以上の成果として、2007年に11編の原著論文を世に出すことができた。また、平成19年度には15件の招待講演を含む46件の学会発表を行った。

Research Products

• [Journal Article] Clay Mimics Color Tuning in Visual Pigments (2007)
  • Author(s): Y. Furutani, et. al.
  • Journal Title: Angew. Chem. Int. Ed. 46 Pages: 8010-8012
  • Peer Reviewed
• [Journal Article] Heterogeneous Environment of the S-H Group of Cys966 near the Flavin Chromophore in the LOV2 Domain of Adiantum Neochromel (2007)
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  • Journal Title: Biochemistry 46 Pages: 10258-10265
  • Peer Reviewed
• [Journal Article] Photochromism of Anabaena Sensory Rhodopsin (2007)
  • Author(s): A. Kawanabe, et. al.
  • Journal Title: J. Am. Chem. Soc. 129 Pages: 8644-8649
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• [Journal Article] Magnetic and Infrared Properties of the Azide Complex of 2,7,12,17-[Tetrapropylporphycenato]iron (III): A Novel Admixing Mechanism of the S=5/2 and S=3/2 States (2007)
  • Author(s): S. Neya, et. al.
  • Journal Title: Eur. J. Inorg. Chem. Pages: 3188-3194
  • Peer Reviewed
• [Journal Article] Halide Binding by the D212N Mutant of Bacteriorhodopsin Affects Hydrogen Bonding of Water in the Active Site (2007)
  • Author(s): M. Shibata, et. al.
  • Journal Title: Biochemistry 46 Pages: 7325-7335
  • Peer Reviewed
• [Journal Article] Hydration and temperature similarly affect light-induced protein structural changes in the chromophoric domain of phototoropin (2007)
  • Author(s): T. Iwata, et. al.
  • Journal Title: Biochemistry 46 Pages: 7016-7021
  • Peer Reviewed
• [Journal Article] Bayesian Maximum Entropy (Two-Diamensional) Lifetime Distribution Reconstruction from Time-Resolved Spectroscopic Data (2007)
  • Author(s): V. A. Lorenz-Fonfria, et. al.
  • Journal Title: Appl. Spectrosc. 61 Pages: 428-443
  • Peer Reviewed
• [Journal Article] FTIR Study of the Retinal Schiff Base and Internal Water Molecules of Proteorhodopsin (2007)
  • Author(s): D. Ikeda, et. al.
  • Journal Title: Biochemistry 46 Pages: 5365-5373
  • Peer Reviewed
• [Journal Article] Early photocycle structural changes in a Bacteriorhodopsin Mutant Engincered to Transmit Photpsensory Signals (2007)
  • Author(s): Y. Sudo, et. al.
  • Journal Title: J. Biol. Chem. 282 Pages: 15550-15558
  • Peer Reviewed
• [Journal Article] Inter-helical Hydrogen Bonds Are Essential Elements for Intra-protein Signal transduction: The Role of Asp115 in Bacteriorhodopsin Transport Function (2007)
  • Author(s): A. Peralvarez-Marin, et. al.
  • Journal Title: J. Mol. Biol. 368 Pages: 666-676
  • Peer Reviewed
• [Journal Article] Practical Aspectsof the maximum Entropy Inversion of the Laplace Transform for Quantitative Analysis of Multi-Exponential Data (2007)
  • Author(s): V. A. Lorenz-Fonfria, et. al.
  • Journal Title: Appl. Spectrosc. 61 Pages: 74-84
  • Peer Reviewed
• [Journal Article] Primary Processes during the light-Signal Transduction of Phototropin (2007)
  • Author(s): D. Matsuoka, et. al.
  • Journal Title: Photochem. Photobiol. 83 Pages: 1-9
  • Peer Reviewed
• [Journal Article] 光を情報へと変換する視物質ロドプシンの光応答機構 (2007)
  • Author(s): 神取 秀樹
  • Journal Title: 化学と教育 55 Pages: 126-129
• [Journal Article] 赤外・ラマン分光法 (2007)
  • Author(s): 古谷 祐詞 神取 秀樹
  • Journal Title: 生物物理学ハンドブック(朝倉書店)
• [Journal Article] 光駆動ポンプ:バクテリオロドプシンの構造・機能 (2007)
  • Author(s): 神取 秀樹
  • Journal Title: 生物物理学ハンドブック(朝倉書店)
• [Journal Article] Photoisomerization Processes in the 13-cis and All-trans Forms of Anabaena Sensory Rhodopsin at 77 K (2006)
  • Author(s): A.Kawanabe et al.
  • Journal Title: Biochemistry 45・14 Pages: 4362-4370
• [Journal Article] Temperature-Dependent Interactions between Photoactivated pharaonis Phoborhodopsin and Its Transducer (2006)
  • Author(s): K.Kamada et al.
  • Journal Title: Biochemistry 45・15 Pages: 4859-4866
• [Journal Article] Assignment of the Hydrogen-Out-Of-Plane and In-Plane Vibrations of the Retinal Chromophore in the K Intermediate of pharaonis Phoborhodopsin (2006)
  • Author(s): Y.Furutani et al.
  • Journal Title: Biochemistry 45・39 Pages: 11836-11843
• [Journal Article] Functional importance of the interhelical hydrogen bond between THR204 and TYR174 of sensory rhodopsin II and its alteration during the signaling process (2006)
  • Author(s): Y.Sudo et al.
  • Journal Title: Journal of Biological Chemistry 281・45 Pages: 34239-34245
• [Journal Article] Conformational Coupling between the Cytoplasmic Carboxylic Acid and the Retinal in a Fungal Light-Driven Proton Pump (2006)
  • Author(s): Y.Furutani et al.
  • Journal Title: Biochemistry 45・51 Pages: 15349-15358
• [Journal Article] Demura and Hideki Kandori, Deprotonation of Glu234 during the Photocycle of Natronomonas pharaonis Halorhodopsin (2006)
  • Author(s): M.Shibata et al.
  • Journal Title: Chem. Phys. Lett. 432 Pages: 545-547
• [Journal Article] Photochemistry in Phototropin, a Blue Light Sensor Protein in Plants (2006)
  • Author(s): T.Iwata, H.Kandori
  • Journal Title: Journal of the Chinese Chemical Society 53巻 Pages: 67-73
• [Journal Article] Photoreaction Cycle of the Light, Oxygen, and Voltage Domain in FKFI Determined by Low-Temperature Absorption Spectroscopy (2006)
  • Author(s): K.Zikihara et al.
  • Journal Title: Biochemistry 45・36 Pages: 10828-10837
• [Journal Article] Identification of the C=O Stretching Vibrations of FMN and Peptide Backbone by ^<13>C-Labeling of the LOV2 domain of Adiantum Phytochrome3 (2006)
  • Author(s): T.Iwata et al.
  • Journal Title: Biochemistry 45・51 Pages: 15384-15391
• [Journal Article] Internal Water Molecules of the Proton-pumping Halorhodopsin in the Presence of Azide. (2006)
  • Author(s): N.Muneda et al.
  • Journal Title: J. Am. Chem. Soc. 128 Pages: 6294-6295
• [Journal Article] Hydrogen-bonding Interaction of the Protonated Schiff Base with Halides in a Chloride-pumping Bacteriorhodopsin Mutant. (2006)
  • Author(s): M.Shibata et al.
  • Journal Title: Biochemistry 45 Pages: 10633-10640
• [Journal Article] Structural Changes in Bacteriorhodopsin Following Retinal Photoisomerization from the 13-cis Form. (2006)
  • Author(s): N.Mizuide et al.
  • Journal Title: Biochemistry 45 Pages: 10674-10681
• [Journal Article] Strongly Hydrogen-bonded Water Molecule is Observed only in the Alkaline Form of Proteorhodopsin. (2006)
  • Author(s): Y.Furutani et al.
  • Journal Title: Chem. Phys. 324 Pages: 705-708
• [Journal Article] Water Structural Changes in the Activation Process of the LOV2 Domain of Adiantum Phytochrome3. (2005)
  • Author(s): Nozaki et al.
  • Journal Title: J.Mol.Struct. 735/736 Pages: 259-265
• [Journal Article] Reactive Cysteine is Protonated in the Triplet Excited State of the LOV2 Domain in Adiantum Phytochrome3. (2005)
  • Author(s): Sato et al.
  • Journal Title: J.Am.Chem.Soc. 127 Pages: 1088-1089
• [Journal Article] Structural Changes of the Complex between pharaonis Phoborhodopsin and Its Cognate Transducer upon Formation of the M Photointermediate. (2005)
  • Author(s): Furutani et al.
  • Journal Title: Biochemistry 44 Pages: 2909-2915
• [Journal Article] FTIR Studies of Internal Water Molecules in the Schiff Base Region of Bacteriorhodopsin. (2005)
  • Author(s): Shibata et al.
  • Journal Title: Biochemistry 44 Pages: 7406-7413
• [Journal Article] Comparative Investigation of the LOV1 and LOV2 Domains in Adiantum Phytochrome3. (2005)
  • Author(s): Iwata et al.
  • Journal Title: Biochemistry 44 Pages: 7427-7434
• [Journal Article] FTIR Studies of the Photoactivation Processes in Squid Retinochrome. (2005)
  • Author(s): Furutani et al.
  • Journal Title: Biochemistry 44 Pages: 7988-7997
• [Journal Article] Unique Temperature Dependence in the Adduct Formation between FMN and Cysteine S-H Group in the LOV2 Domain of Adiantum Phytochrome3. (2005)
  • Author(s): Nozaki et al.
  • Journal Title: Chem.Phys.Lett. 410 Pages: 59-63
• [Journal Article] Hydrogen-Bonding Alterations of the Protonated Schiff Base and Water Molecule in the Chloride Pump of Natronobacterium pharaonis. (2005)
  • Author(s): Shibata et al.
  • Journal Title: Biochemistry 44 Pages: 12279-12286
• [Journal Article] FTIR Spectroscopy of the All-Trans Form of Anabaena Sensory Rhodopsin at 77 K : Hydrogen Bond of a Water between the Schiff Base and Asp75. (2005)
  • Author(s): Furutani et al.
  • Journal Title: Biochemistry 44 Pages: 12287-12296
• [Journal Article] Strongly Hydrogen-Bonded Water Molecule Present near the Retinal Chromophore of Leptosphaeria Rhodopsin, the Bacteriorhodopsin-like Proton Pump from a Eukaryote. (2005)
  • Author(s): Sumii et al.
  • Journal Title: Biochemistry 44 Pages: 15159-15166
• [Journal Article] Redox-induced Protein Structural Changes in Cytochrome bo Revealed by Fourier-transform Infrared Spectroscopy and ^<13>C-Tyr-labeling. (2005)
  • Author(s): Kandori et al.
  • Journal Title: J.Biol.Chem. 280 Pages: 32821-32826
• [Journal Article] Steric Constraint in the Primary Photoproduct of an Archaeal Rhodopsin from Regiospecific Perturbation of C-D Stretching Vibration of the Retinyl Chromophore. (2005)
  • Author(s): Sudo et al.
  • Journal Title: J.Am.Chem.Soc. 127 Pages: 16036-16037
• [Journal Article] Strongly Hydrogen-Bonded Water Molecules in the Schiff Base Region of Rhodopsins. (2005)
  • Author(s): Furutani et al.
  • Journal Title: Photochem.Photobiol.Sci. 4 Pages: 661-666
• [Journal Article] Mechanism of Photoactivation in the LOV2 Domain of Adiantum Phytochrome3. (2005)
  • Author(s): Iwata et al.
  • Journal Title: Recent Res.Devel.Biochem. 6 Pages: 1-26
• [Journal Article] イオンポンプの機能にかかわる蛋白質内部の水分子 (2005)
  • Author(s): 神取秀樹
  • Journal Title: 現代化学 414 Pages: 51-57
• [Journal Article] Water structural changes in the activation process of the LOV2 domain of Adiantum phytochrome3 (2005)
  • Author(s): D.Nozaki et al.
  • Journal Title: J.Mol.Struct. 735 Pages: 259-265
• [Journal Article] Reactive cysteine is protonated in the triplet excited state of the LOV2 domain in Adiantum phytochrome3 (2005)
  • Author(s): Y.Sato et al.
  • Journal Title: J.Am.Chem.Soc. 127 Pages: 1088-1089
• [Journal Article] Structural changes of the complex between pharaonis phoborhodopsin and its cognate transducer upon formation of the M photointermediate (2005)
  • Author(s): Y.Furutani et al.
  • Journal Title: Biochemistry 44(In press)
• [Journal Article] Strongly hydrogen-bonded water molecules in the Schiff base region of rhodopsins (2005)
  • Author(s): Y.Furutani et al.
  • Journal Title: Photochem.Photobiol.Sci. (In press)
• [Journal Article] FTIR spectroscopy of the O photointermedate in pharaonis phoborhodopsin (2004)
  • Author(s): Y.Furutani et al.
  • Journal Title: Biochemistry 43 Pages: 5204-5212
• [Journal Article] Role of Gln1029 in the photoactivation processes of the LOV2 domain in Adiantum phytochrome3 (2004)
  • Author(s): D.Nozaki et al.
  • Journal Title: Biochemistry 43 Pages: 8373-8379
• [Journal Article] Internal water molecules of light-driven chloride pump proteins (2004)
  • Author(s): M.Shibata et al.
  • Journal Title: Chem.Phys.Lett. 392 Pages: 330-333
• [Journal Article] Altered hydrogen bonding of Arg82 during the proton pump cycle of bacteriorhodopsin : A low-temperature polarized FTIR spectroscopic study (2004)
  • Author(s): T.Tanimoto et al.
  • Journal Title: Biochemistry 43 Pages: 9439-9447
• [Journal Article] FTIR spectroscopy of the K photointermedate of neurospora rhodopsin : Structural changes of the retinal, protein, and water molecules after photoisomerization (2004)
  • Author(s): Y.Furutani et al.
  • Journal Title: Biochemistry 43 Pages: 9636-9646
• [Journal Article] Role of hydrogen-bond network in energy storage of bacteriorhodopsin's light-driven proton pump revealed by ab initio normal-mode analysis (2004)
  • Author(s): S.Hayashi et al.
  • Journal Title: I.Am.Chem.Soc. 126 Pages: 10516-10517
• [Journal Article] Hydration switch model for the proton transfer in the Schiff base region of bacteriorhodopsin (2004)
  • Author(s): H.Kandori
  • Journal Title: Biochim.Biophys.Acta 1658 Pages: 72-79
• [Journal Article] Molecular mechanism of protein-protein interaction of pharaonis phoborhodopsin/transducer and photo-signal transfer reaction by complex (2004)
  • Author(s): Y.Sudo et al.
  • Journal Title: Recent Res.Devel.Biophys. 3 Pages: 1-16
• [Journal Article] バクテリオロドプシンにおける内部結合水の振動解析 (2004)
  • Author(s): 柴田幹大, 神取秀樹
  • Journal Title: 生物物理 44 Pages: 113-117
  • NAID: 110001151703
• [Presentation] レチナールの色と反応を制御するロドプシンという蛋白質場の役割 (2007)
  • Author(s): 神取秀樹
  • Organizer: 理化学研究所研究会「分子系の構造と電子状態-『生物物質科学』を目指して
  • Place of Presentation: 和光
• [Presentation] ロドブシンの赤外分光法:より生理的な測定を目指して (2007)
  • Author(s): 神取秀樹
  • Organizer: 分子科学研究所研究会「生細胞の分子科学」
  • Place of Presentation: 岡崎
• [Presentation] FTIR study of rhodopsins in action (2007)
  • Author(s): Hideki Kandori
  • Organizer: LALS 2007, International Conference on Laser Applications in Life Sciences
  • Place of Presentation: Moscow, Russia
• [Presentation] 膜蛋白質におけるプロトン伝導 (2007)
  • Author(s): 神取秀樹
  • Organizer: 生理学研究所研究会「膜機能分子ダイナミクスの分子機構解明に向けて
  • Place of Presentation: 岡崎
• [Presentation] FTIR Study of Proton Pumping and Light-Signal Transduction Mechanisms in Archaeal-Type Rhodopsins (2007)
  • Author(s): Yuji Furutani and Hideki Kandori
  • Organizer: 9th International Symposium on Biotechnology Metal Complexes and Catalysis (BMC-IX)
  • Place of Presentation: Xilinhot, Inner Mongolia, China
• [Presentation] FTIR study of photoreceptive proteins in action (2007)
  • Author(s): Hideki Kandori
  • Organizer: International Workshop of the Collaborative Research Center on "Protein-Cofactor Interactions in Biological Processes
  • Place of Presentation: Berlin, Germany
• [Presentation] Determinants of Light-Energy and Light-Signal Conversions in Rhodopsins (2007)
  • Author(s): Hideki Kandori
  • Organizer: International Symposium on "Retinal Proteins: Experiments and Theory"
  • Place of Presentation: Bremen, Germany
• [Presentation] FTIR Studies of Internal Water Molecules of Bacteriorhodopsin: Structural Analysis of Halide-bound D85S and D212N Muntants inthe Schiff Base Region (2007)
  • Author(s): Mikihiro Shibata and Hideki Kan dori
  • Organizer: International Conference of Computational Methods in Sciences and Engineering
  • Place of Presentation: Corf, Greece
• [Presentation] The Determinant of Light-Energy and Light-Signal Conversion in Rhodopsins (2007)
  • Author(s): Hideki Kandori
  • Organizer: International Conference of Computational Methods in Sciences and Engineering
  • Place of Presentation: Corf, Greece
• [Presentation] Structural changes of water molecules upon the reduction of quinones in the reaction center from Rhodobacter sphaeroides (2007)
  • Author(s): Tatsuya Iwata, Mark L. Paddock, Melvin Y. Okamura and Hideki Kandori
  • Organizer: 17th International Symposium on Fine Chemistry and Functional Polymers & IUPAC 3rd International Symposium on Novel Materialsand Synthesis
  • Place of Presentation: Shanghai, China
• [Presentation] The determinants of light-energy and light-signal conversion in rhod opsins (2007)
  • Author(s): Hideki Kandori
  • Organizer: Molecular Science and Chemical Biology of Biomolecular Function
  • Place of Presentation: Okazaki, Japan
• [Presentation] 水分子の水素結合強度が決めるロドプシンの機能 (2007)
  • Author(s): 神取秀樹
  • Organizer: 赤外ラマン分光部会シンポジウム
  • Place of Presentation: 豊中
• [Presentation] 赤外分光によるプロトンポンプ蛋白質のメカニズム研究 (2007)
  • Author(s): 神取秀樹
  • Organizer: 水素量子アトミクス研究会
  • Place of Presentation: 札幌
• [Presentation] 赤外分光法によるタンパク質間相互作用の解析-ロドプシンをモデルとして- (2007)
  • Author(s): 古谷祐詞、神取秀樹
  • Organizer: 日本生物物理学会第45回年会
  • Place of Presentation: 横浜
• [Presentation] 分子ポンプを考える (2007)
  • Author(s): 神取秀樹
  • Organizer: 日本生物物理学会第45回年会
  • Place of Presentation: 横浜
• [Presentation] 紅色細菌の光化学反応中心のキノン還元に伴う内部結合水の構造変化 (2007)
  • Author(s): 岩田達也、Mark L. Paddock、Melvin Y. Okamura、神取秀樹
  • Organizer: 第34回生体分子科学討論会
  • Place of Presentation: 仙台
• [Presentation] 赤外分光法によるセンサリーロドプシンIの光情報伝達過程での構造変化および相互作用変化の解析 (2007)
  • Author(s): 古谷祐詞、高橋はづき、佐々木純、須藤雄気、John L. Spudich、神取秀樹
  • Organizer: 第34回生体分子科学討論会
  • Place of Presentation: 仙台
• [Presentation] FTIR分光法によるGloeobacter rhodopsinの初期中間体における構造変化の研究 (2007)
  • Author(s): 橋本匡平、古谷祐詞、Kwang-Hwan Jung、神取秀樹
  • Organizer: 第14回日本光生物学協会年会
  • Place of Presentation: 奈良
• [Presentation] 硝酸イオン結合型ファラオニスハロロドプシンの低温赤外分光 (2007)
  • Author(s): 中島啓介、柴田幹大、出村誠、神取秀樹
  • Organizer: 第14回日本光生物学協会年会
  • Place of Presentation: 奈良
• [Presentation] 赤外分光法によるセンサリーロドプシンIの光情報伝達過程におけるタンパク質間相互作用の解析 (2007)
  • Author(s): 高橋はづき、古谷祐司、佐々木 純、須藤雄気、Jhon L. Spudich、神取秀樹
  • Organizer: 第14回日本光生物学協会年会
  • Place of Presentation: 奈良
• [Presentation] ホウライシダneochrome1-LOV2ドメインの光誘起構造変化におけるPhe-1010の役割 (2007)
  • Author(s): 山本渥史、岩田達也、徳富 哲、神取秀樹
  • Organizer: 第14回日本光生物学協会年会
  • Place of Presentation: 奈良
• [Presentation] Anabaena sensory rhodopsinの光反応過程の研究 (2007)
  • Author(s): 川鍋陽、古谷祐詞、Kwang-Hwan Jung、神取秀樹
  • Organizer: 第14回日本光生物学協会年会
  • Place of Presentation: 奈良
• [Presentation] 赤外分光法によるハロロドプシンの細胞外側領域の構造解析 (2007)
  • Author(s): 柴田幹大、出村誠、神取秀樹
  • Organizer: 第14回日本光生物学協会年会
  • Place of Presentation: 奈良
• [Presentation] 光センサーに変換したバクテリオロドプシンの構造と構造変化 (2007)
  • Author(s): 須藤雄気、古谷祐詞、神取秀樹、John L. Spudich
  • Organizer: 第14回日本光生物学協会年会
  • Place of Presentation: 奈良
• [Presentation] 自己組織化により形成されたM6L4型かご状錯体内部に存在する水クラスターの光化学反応に対する赤外分光法による解析 (2007)
  • Author(s): 古谷祐詞、吉沢道人、藤田誠、神取秀樹
  • Organizer: 第1回分子科学討論会
  • Place of Presentation: 仙台
• [Presentation] 2,7,12,17-テトラプロピルポルフィセン鋲(III)錯体に結合したアジドの温度依存的な赤外吸収変化の解析によるスピン状態の考察 (2007)
  • Author(s): 古谷祐詞、神取秀樹、池崎章、大胡惠樹、中村幹夫、根矢三郎
  • Organizer: 第57回錯体化学討論会
  • Place of Presentation: 名古屋
• [Presentation] Altered Hydrogen Bonding of the Arg123 and Water Molecules in the Lland L2-intermediate of pharaonis Halorhodonsin (2007)
  • Author(s): Mikihiro Shibata, Makoto Demura and Hideki Kandori
  • Organizer: Projects of the SFb-498 "Protein-Cofactor Interactions in Biological Processes"
  • Place of Presentation: Berlin, Germany
• [Presentation] Altered Hydrogen Bonding of the Arg123 and Water Molecules in the L1 and L2-intermediate of pharaonis Halorhodopsin (2007)
  • Author(s): Mikihiro Shibata, Makoto Demur a and Hideki kandori
  • Organizer: International Symposium on Retinal Proteins: Experim ents and Theory
  • Place of Presentation: Berlin, Germany
• [Presentation] FTIR Study of Nitrate-bound pharaonis Halorhodopsin (2007)
  • Author(s): Keisuke Nakashima, Mikihiro Shibata, Makoto Demura and Hideki Kandori
  • Organizer: Molecular Science and Chemical Biology of Biom olecular Function
  • Place of Presentation: Okazaki, Japan
• [Presentation] Long-Ranged Protein Structural Changes upon Retinal Photoisomerization in a Cyanobacterial Rhodopsin Studied by FTIR Spectroscopy (2007)
  • Author(s): Kyohei Hashimoto, Yuji Furutani, Ah Reum Choi, Kwang-Hwan Jung and Hideki Kandori
  • Organizer: Molecular Science and Chemical Biology of Biomolecular Function
  • Place of Presentation: Okazaki, Japan
• [Presentation] Interaction Changes of Sensory Rhodopsin with its Transducer Protein upon Formation of the S373 Intermediate Studied by FTIR Spectroscopy (2007)
  • Author(s): Hazuki Takahashi, Yuji Furutani, Jun Sasaki, Yuki Sudo, John L. Spudich and Hideki Kandori
  • Organizer: Molecular Science and Chemical Biology Of Biom olecular Function
  • Place of Presentation: Okazaki, Japan
• [Presentation] Protein-Protein Interactions In pharaonis Phoborhodopsin-pHtrII Complexunder Aqueous Environment Studied by ATR-FTIR Spectroscopy (2007)
  • Author(s): Yuya Kitade, Yuji Furutani, Yuki Sudo, Naoki Kamo, and Hideki kandori
  • Organizer: Molecular Science and Chemical Biology of Biom olecular Function
  • Place of Presentation: Okazaki, Japan
• [Presentation] Steric Constraint at the C14 Position of the Retinal Chromophore Initiates Light-Signal Transduction of pharaonis Sensory Rhodopsin II (2007)
  • Author(s): Motohiro Ito, Yuki Sudo, Yuji Furutani, Takashi Okitsu, Akimori Wada, Michio Homma, Jhon L. Spudich. and Hideki Kandori
  • Organizer: Molecular Science and Chemical Biology of Biomolecular Function
  • Place of Presentation: Okazaki, Japan
• [Presentation] Halide Binding by D85S and D212N Bacteriorhodopsin Mutants Affects Hydrogen-Bonding Network in the Active Site (2007)
  • Author(s): Mikihiro shibata, Maiko Yoshitsugu, Kunio Ihara and Hideki Kandori
  • Organizer: Molecular Science and Chemical Biology Of Biomolecular Function
  • Place of Presentation: Okazaki, Japan
• [Presentation] Gloeobacter rhodopsinにおけるレチナールの光異性化にともなうプロトンドナーの構造変化 (2007)
  • Author(s): 橋本 匡平、古谷 祐詞、Ah Reum Choi、Kwang-Hwan Jung、神取秀樹
  • Organizer: 日本生物物理学会第45回年会
  • Place of Presentation: 横浜
• [Presentation] 硝酸イオン結合型ファラオニスハロロドプシンの赤外分光 (2007)
  • Author(s): 中島啓介、柴田幹大、出村誠、神取秀樹
  • Organizer: 日本生物物理学会第45回年会
  • Place of Presentation: 横浜
• [Presentation] 赤外分光法によるセンサリーロドプシンIの赤色光照射時の構造変化および相互作用の変化 (2007)
  • Author(s): 高橋はづき、古谷祐詞、佐々木純、須藤雄気、John L.Spudich、神取秀樹
  • Organizer: 日本生物物理学会第45回年会
  • Place of Presentation: 横浜
• [Presentation] 種々のフォトトロピンにおけるLOV2ドメインとJα-helixの光誘起構造変化 (2007)
  • Author(s): 小山貴之、山本渥史、岩田達也、佐藤義彰、松岡大介、徳富哲、神取秀樹
  • Organizer: 日本生物物理学会第45回年会
  • Place of Presentation: 横浜
• [Presentation] ファラオニスフォボロドプシンの「水中」における膜タンパク質問相互作用の全反射赤外分光法による解析 (2007)
  • Author(s): 北出祐也、古谷祐詞、加茂直樹、神取秀樹
  • Organizer: 日本生物物理学会第45回年会
  • Place of Presentation: 横浜
• [Presentation] 乾電池型プロテオロドプシン変異体の研究 (2007)
  • Author(s): 吉次麻衣子、池田大亮、柴田幹大、古谷祐詞、神取秀樹
  • Organizer: 日本生物物理学会第45回年会
  • Place of Presentation: 横浜
• [Presentation] シロイヌナズナphot1におけるLOV2ドメインからJαヘリックスへの光誘起構造変化の伝達メカニズム (2007)
  • Author(s): 山本渥史、岩田達也、佐藤義彰、松岡大介、徳富 哲、神取秀樹
  • Organizer: 日本生物物理学会第45回年会
  • Place of Presentation: 横浜
• [Presentation] ファラオニスセンサリーロドプシンIIの光情報伝達を開始させる特異なタンパク質-発色団相互作用について (2007)
  • Author(s): 伊藤元博、須藤雄気、古谷祐詞、沖津貴志、和田昭盛、John. L. Spudich、神取秀樹
  • Organizer: 日本生物物理学会第45回年会
  • Place of Presentation: 横浜
• [Presentation] Anabaena sensory rhodopsinのL中間体におげる細胞質側での構造変化 (2007)
  • Author(s): 川鍋陽、古谷祐詞、Kwang-Hwan Jung、神取秀樹
  • Organizer: 日本生物物理学会第45回年会
  • Place of Presentation: 横浜
• [Presentation] ファラオニスハロロドプシンのクロライドポンプにおけるプロリン残基の役割 (2007)
  • Author(s): 柴田幹大、出村誠、神取秀樹
  • Organizer: 日本生物物理学会第45回年会
  • Place of Presentation: 横浜
• [Presentation] 紅色光合成細菌の反応中心のQB周囲の内部結合水 (2007)
  • Author(s): 岩田達也、Mark L. Paddock、Melvin Y. Okamura、神取秀樹
  • Organizer: 日本生物物理学会第45回年会
  • Place of Presentation: 横浜
• [Presentation] ウシロドプシンのレチナール結合部位の構造安定性の解析-Thr118のH/D交換実験 (2007)
  • Author(s): 古谷祐詞、Victor Lorenz-Fonfria、太田徹、神取秀樹
  • Organizer: 日本生物物理学会第45回年会
  • Place of Presentation: 横浜
• [Book] cis-trans Isomerization in Biochemistry (2006)
  • Author(s): H.Kandori
  • Publisher: Retinal binding proteins
• [Book] 細胞生物学事典(イオンポンプ) (2005)
  • Author(s): 神取秀樹
  • Total Pages: 2
  • Publisher: 朝倉書店
• [Remarks]
  • URL: http://www.ach.nitech.ac.jp/~physchem/kandori/index_j.html
• [Publications] T.Tanimoto et al.: "Structural Changes of Water in the Schiff Base Region of Bacteriorhodopsin : Proposal of a Hydration Switch Model"Biochemistry. 42. 2300-2306 (2003)
• [Publications] M.Iwamoto et al.: "Proton Transfer Reactions in the F86D and F86E Mutants of Pharaonis Phoborhodopsin (Sensory Rhodopsin II)"Biochemistry. 42. 2790-2796 (2003)
• [Publications] Y.Furutani et al.: "FTIR Spectroscopy of the complex between pharaonis Phoborhodopsin and its Transducer Protein"Biochemistry. 42. 4837-4842 (2003)
• [Publications] K.Shimono et al.: "Vibrational Modes of the Protonated Schiff Base in pharaonis Phoborhodopsin"Biochemistry. 42. 7801-7806 (2003)
• [Publications] T.Iwata et al.: "Light-induced structural changes in the LOV2 domain of Adiantum phytochrome3 studied by low-temperature FTIR and UV-visible spectroscopy"Biochemistry. 42. 8183-8191 (2003)
• [Publications] Y.Furutani et al.: "Structural Changes in Lumirhodopsin and Metarhodopsin I Studied by their Photoreactions at 77 K"Biochemistry. 42. 8494-8500 (2003)
• [Publications] Y.Furutani et al.: "Structural Changes of Water Molecules during the Photoactivation Processes in Bovine Rhodopsin"Biochemistry. 42. 9619-9625 (2003)
• [Publications] M.Shibata et al.: "Water molecules in the Schiff Base Region of Bacteriorhodopsin"J.Am.Chem.Soc.. 125. 13312-13313 (2003)
• [Publications] Y.Sudo et al.: "Hydrogen bonding alteration of Thr204 in the complex between pharaonis phoborhodopsin and its transducer protein"Biochemistry. 42. 14166-14172 (2003)
• [Publications] Y.Furutani et al.: "FTIR Spectroscopy of the O Photointermediate in pharaonis Phoborhodopsin"Biochemistry. (発表予定). (2004)
• [Publications] M.Iwamoto et al.: "Photochemical properties of pharaonis phoborhodopsin (sensory rhodopsin II)"Recent Res.Develp.Chem.. 1. 15-30 (2003)
• [Publications] 神取秀樹: "視覚の初期過程における光化学反応"レーザー研究. 31. 184-189 (2003)
• [Publications] 神取秀樹: "ロドプシンを究める"日本化学会、生体機能関連化学部会 News Letter. 17. 10-13 (2004)
• [Publications] H.Kandori: "Hydration Switch Model for the proton transfer in the Schiff base region of bacteriorhodopsin"Biochim.Biophys.Acta. (In press). (2004)
• [Publications] 柴田幹大, 神取秀樹: "バクテリオロドプシンにおける内部結合水の振動解析"生物物理. (印刷中). (2004)