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植物の機能発現とメンブレントラフィック-酵母研究からの展開

Research Project

Project/Area Number 15079210
Research Category

Grant-in-Aid for Scientific Research on Priority Areas

Allocation TypeSingle-year Grants
Review Section Biological Sciences
Research InstitutionThe University of Tokyo

Principal Investigator

中野 明彦  The University of Tokyo, 大学院・理学系研究科, 教授 (90142140)

Co-Investigator(Kenkyū-buntansha) 上田 貴志  東京大学, 大学院・理学系研究科, 准教授 (10311333)
森田 美代  奈良先端科学技術大学院大学, バイオサイエンス研究科, 准教授 (10314535)
東山 哲也  東京大学, 大学院理学系研究科, 助手 (00313205)
Project Period (FY) 2003 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥51,200,000 (Direct Cost: ¥51,200,000)
Fiscal Year 2007: ¥9,000,000 (Direct Cost: ¥9,000,000)
Fiscal Year 2006: ¥9,400,000 (Direct Cost: ¥9,400,000)
Fiscal Year 2005: ¥9,400,000 (Direct Cost: ¥9,400,000)
Fiscal Year 2004: ¥11,700,000 (Direct Cost: ¥11,700,000)
Fiscal Year 2003: ¥11,700,000 (Direct Cost: ¥11,700,000)
KeywordsRab5 GTPase / エンドソーム / 情報伝達 / ARA6 / ARA7 / RHA1 / ヌクレオチド交換因子 / ゴルジ体 / Rab GTPase / エンドサイトーシス / Rab5 / GTP / GDP交換因子 / SNARE / 胚発生 / 液胞 / GDP / GTP交換因子 / 融合 / 重力屈性 / Ara6 / Ara7 / リサイクリング / アクチン繊維
Research Abstract

主にシロイヌナズナを材料に用い,次のような研究を行った。
1.シロイヌナズナのRab5 GTPaseのうち,種を越えてよく保存されているタイプのARA7,RHA1の二重欠失変異体が雄性不稔となる原因について検討し,花粉の出芽・伸長過程に異常がある可能性を示唆した。また,そのグアニンヌクレオチド交換因子であるVPS9aとVPS9bのうち,前者は全ての器官・組織で,後者は花器官でのみ発現していることを示し,配偶体機能におけるこれらのRab5 GTPaseの重要性を示唆した。一方,植物特異的なRab5 GTPaseであるARA6についても解析を進め,ARA7/RHA1とARA6が,地上部の成長,花成,根の伸長などさまざまな機能において拮抗的に働くことを明らかにした。
2.Rab5 GTPaseが下流現象を引き起こす上で重要なエフェクター分子を,酵母2ハイブリッド法を用いて探索した。ARA7/RHA1とARA6とで全く異なるエフェクター候補が得られ,異なるエンドソームシグナリングを介して拮抗的な機能を果たしている可能性を示唆した。
3.シロイナズナのゴルジ体のシス領域とトランス領域を異なる蛍光タンパク質で標識し,ライブイメージングを行うことによって,ゴルジ体のユニット間でダイナミックな膜のやり取りがあること,チューブ状の構造が頻繁に形成されることなどを明らかにした。また,そのようなチューブ構造の形成と維持に異常を示す突然変異体を単離した。

Report

(5 results)
  • 2007 Annual Research Report
  • 2006 Annual Research Report
  • 2005 Annual Research Report
  • 2004 Annual Research Report
  • 2003 Annual Research Report
  • Research Products

    (43 results)

All 2008 2007 2006 2005 2004 Other

All Journal Article (28 results) (of which Peer Reviewed: 7 results) Presentation (10 results) Publications (5 results)

  • [Journal Article] Auxin transport inhibitors impair vesicle motility and actin cytoskeleton dynamics in diverse eukaryotes.2008

    • Author(s)
      Dhonukshe, P.
    • Journal Title

      Proc. Natl. Acad. Sci. USA 105

      Pages: 4489-4494

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] In vitro formation of a novel type of membrane vesicles containing Dpmlp: putative transport vesicles for lipid droplets in budding yeast.2008

    • Author(s)
      Takeda, Y.
    • Journal Title

      J. Biochem. (掲載確定)(印刷中)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The EMBRYO YELLOW gene, encoding a subunit of the conserved oligomeric Golgi complex, is required for appropriate cell expansion and meristem organization in Arabidopsis thaliana.2008

    • Author(s)
      Ishikawa, T.
    • Journal Title

      Genes Cells (掲載確定)(印刷中)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Phospholipid mediated plasticity in PC12 cells.2007

    • Author(s)
      Uchiyama, Y.
    • Journal Title

      Brain Res. 1151

      Pages: 46-54

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] endodermal-amyloplast less l is a novel allele of SHORT-ROOT.2007

    • Author(s)
      Morita, M. T.
    • Journal Title

      Advances Spase Res. 39

      Pages: 1127-1133

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] VPS9a, the common activator for two distinct types of Rab5 GTPases, is essential for plant development.2007

    • Author(s)
      Goh, T.
    • Journal Title

      Plant Cell 19

      Pages: 3504-3515

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Smy2p participates in coat protein complex II(COPII)vesicle formation through the interaction with Sec23p/Sec24p subcomplex in Saccharomyces cerevisiae.2007

    • Author(s)
      Higashio, H.
    • Journal Title

      Traffic 9

      Pages: 79-93

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The assembly pathway of the 19S regulatory particle of the yeast 26S proteasome.2007

    • Author(s)
      Isono, E.
    • Journal Title

      Mol. Biol.Cell 18

      Pages: 569-580

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Phospholipid mediated plasticity in exocytosis observed in PC12 cells.2007

    • Author(s)
      Uchiyama, Y.
    • Journal Title

      Brain Res. (印刷中)

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Mechanisms of COPII vesicle formation and protein sorting.2007

    • Author(s)
      Sato, K.
    • Journal Title

      FEBS Lett. (印刷中)

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Structures of carbohydrate recognition domain of Ca^<2+> -independent cargo receptors Emp46p and Emp47p.2006

    • Author(s)
      Satoh, T.
    • Journal Title

      J. Biol. Chem. 281

      Pages: 10410-10419

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Live imaging of yeast Golgi cisternal maturation.2006

    • Author(s)
      Matsuura-Tokita, K.
    • Journal Title

      Nature 441

      Pages: 1007-1010

    • Related Report
      2006 Annual Research Report
  • [Journal Article] An Arflp GTPase-activating protein, Glo3p, executes its regulatory function through the Glo3 motif at its C-terminus.2006

    • Author(s)
      Yahara, N.
    • Journal Title

      J. Cell Sci. 119

      Pages: 2604-2612

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Species preferentiality of the pollen tube attractant derived from the synergid cell of Torenia fournieri.2006

    • Author(s)
      Higashiyama, T.
    • Journal Title

      Plant Physiol 142

      Pages: 481-491

    • Related Report
      2006 Annual Research Report
  • [Journal Article] A C2H2-type zinc finger protein, SGR5, is involved in early events of gravitropism in Arabidopsis inflorescence stems.2006

    • Author(s)
      Morita, M.
    • Journal Title

      Plant J. 47

      Pages: 619-628

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Active digestion of sperm mitochondrial DNA in single living sperm revealed by optical tweezers.2006

    • Author(s)
      Nishimura, Y.
    • Journal Title

      Proc.Natl.Acad.Sci.U.S.A. 103

      Pages: 1382-1387

    • Related Report
      2005 Annual Research Report
  • [Journal Article] GENERATIVE CELL SPECIFIC 1 is essential for angiosperm fertilization.2006

    • Author(s)
      Mori, T.
    • Journal Title

      Nat.Cell Biol. 8

      Pages: 64-71

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Conversion of functional specificity in Qb-SNARE VTI1 homologues of Arabidopsis.2005

    • Author(s)
      Niihama, M.
    • Journal Title

      Curr.Biol. 15

      Pages: 555-560

    • Related Report
      2005 Annual Research Report
  • [Journal Article] VAN3 ARF GAP-mediated vesicle transport involved in leaf vascular network2005

    • Author(s)
      Koizumi, K.
    • Journal Title

      Developoment 132

      Pages: 1699-1711

    • Related Report
      2005 Annual Research Report
  • [Journal Article] DRP1A is responsible for vascular continuity synergistically working with VAN3 in Arabidopsis.2005

    • Author(s)
      Sawa, S.
    • Journal Title

      Plant Physiol. 138

      Pages: 819-826

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Actin-based motility of endosomes is linked to the polar tip growth of root hairs.2005

    • Author(s)
      Voigt, B.
    • Journal Title

      Eur.J.Cell Biol. 84

      Pages: 609-621

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Shoot circumnutation and winding movements require gravisensing cells.2005

    • Author(s)
      Kitazawa, D.
    • Journal Title

      Proc.Natl.Acad.Sci.U.S.A. 102

      Pages: 18742-18747

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Amyloplasts and vacuolar membrane dynamics in the living graviperceptive cell of the Arabidopsis inflorescence stem.2005

    • Author(s)
      Saito, C.
    • Journal Title

      Plant Cell 17

      Pages: 548-558

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Conversion of functional specificity in Qb-SNARE VTI1 homologues of Arabidopsis.2005

    • Author(s)
      Niihama, M.
    • Journal Title

      Curr.Biol. (印刷中)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] VAN3 ARF GAP-mediated vesicle transport involved in leaf vascular network formation.2005

    • Author(s)
      Koizumi, K.
    • Journal Title

      Developoment (印刷中)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Systematic analysis of SNARE molecules in Arabidopsis : Dissection of post-Golgi network in plant cells.2004

    • Author(s)
      Uemura, T.
    • Journal Title

      Cell Struct.Funct. 29

      Pages: 49-65

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Functional differentiation of endosomes in Arabidopsis cells.2004

    • Author(s)
      Ueda, T.
    • Journal Title

      Plant J. 40

      Pages: 783-789

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Genome sequence of the ultrasmall unicellular red alga Cyanidioschyzon merolae 10D.2004

    • Author(s)
      Matsuzaki, M.
    • Journal Title

      Nature 428

      Pages: 653-657

    • Related Report
      2004 Annual Research Report
  • [Presentation] 植物細胞のエンドサイトーシスで働くAra7/AtVps9aの複合体の結晶構造解析2007

    • Author(s)
      上島珠美
    • Organizer
      日本分子生物学会・日本生化学会合同大会
    • Place of Presentation
      横浜
    • Year and Date
      2007-12-10
    • Related Report
      2007 Annual Research Report
  • [Presentation] シロイヌナズナの重力感受におけるオルガネラ動態の制御機構2007

    • Author(s)
      森田(寺尾)美代
    • Organizer
      バイオアーキテクトシンポジウム
    • Place of Presentation
      和光
    • Year and Date
      2007-09-11
    • Related Report
      2007 Annual Research Report
  • [Presentation] 植物における小胞輸送の分子機構と高次機能発現における役割の研究2007

    • Author(s)
      上田貴志
    • Organizer
      日本植物学会第71回大会
    • Place of Presentation
      野田
    • Year and Date
      2007-09-08
    • Related Report
      2007 Annual Research Report
  • [Presentation] Ara6エンドソームを介した植物特異的な情報伝達機構の解析2007

    • Author(s)
      伊藤瑛海
    • Organizer
      日本植物学会第71回大会
    • Place of Presentation
      野田
    • Year and Date
      2007-09-08
    • Related Report
      2007 Annual Research Report
  • [Presentation] ゴルジ体とタンパク質輸送:論争解決(?)後も謎は深まるばかり2007

    • Author(s)
      中野明彦
    • Organizer
      第27回日本糖質学会年会
    • Place of Presentation
      福岡
    • Year and Date
      2007-08-03
    • Related Report
      2007 Annual Research Report
  • [Presentation] State-of-the-art imaging of the secretory pathway: from the ER and through the Golgi2007

    • Author(s)
      Nakano, A.
    • Organizer
      Gordon Research Conference
    • Place of Presentation
      Andover, NH, USA
    • Year and Date
      2007-07-12
    • Related Report
      2007 Annual Research Report
  • [Presentation] Roles of membrane traffic in xylem differentiation2007

    • Author(s)
      Ito, J.
    • Organizer
      18th Intl. Conference on Arabidopsis Research
    • Place of Presentation
      Beijing, China
    • Year and Date
      2007-06-20
    • Related Report
      2007 Annual Research Report
  • [Presentation] Roles of endocytosis regulation in plant physiology and development2007

    • Author(s)
      Nakano, A.
    • Organizer
      3rd Intl. Symposium on Plant Neurobiology
    • Place of Presentation
      Strbske pleso, Slovakia
    • Year and Date
      2007-05-15
    • Related Report
      2007 Annual Research Report
  • [Presentation] 高速高感度共焦点顕微鏡による微小オルガネラのライブイメージング-光学顕微鏡の限界への挑戦-2007

    • Author(s)
      中野明彦
    • Organizer
      日本動物学会第78回大会
    • Place of Presentation
      弘前
    • Year and Date
      2007-05-15
    • Related Report
      2007 Annual Research Report
  • [Presentation] 高速高感度CLSMによる超高分解能ライブセルイメージング2007

    • Author(s)
      中野明彦
    • Organizer
      第27回日本医学会総会
    • Place of Presentation
      大阪
    • Year and Date
      2007-04-06
    • Related Report
      2007 Annual Research Report
  • [Publications] Geldner, N.: "The Arabidopis GNOM ARF-GEF mediates endosome recycling, auxin transport, and auxin-dependent plant growth"Cell. 112. 219-230 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Grebe, M.: "Arabidopsis sterol endocytosis involves actin-mediated trafficking via ARA6-positive early endosomes"Curr.Biol.. 13. 1378-1335 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Surpin, M.: "The VTI family of SNARE proteins is necessary for plant viability and mediates different protein transport pathways"Plant Cell. 15. 2885-2899 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Yano, D.: "A SNARE complex containing SGR3/AtVAM3 and ZIG/VTI11 in gravity-sensing cells is important for Arabidopsis shoot gravitropism"Proc.Natl.Acad.Sci.U.S.A.. 100. 8589-8594 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Nakano, A.: "Yeast Golgi apparatus - dynamics and sorting"Cell.Mol.Life Sci.. 61. 186-192 (2004)

    • Related Report
      2003 Annual Research Report

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Published: 2003-04-01   Modified: 2018-03-28  

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