ニューロン-グリア相互作用に関与する機能分子の探索

• Project/Area Number: 15082209
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: National Institute for Physiological Sciences (2004-2007); Okazaki National Research Institutes (2003)
• Principal Investigator: 池中 一裕 National Institute for Physiological Sciences, 分子生理研究系, 教授 (00144527)
• Co-Investigator(Kenkyū-buntansha): 小野 勝彦 生理学研究所, 分子生理研究係, 准教授 (30152523) ; 等 誠司 生理学研究所, 分子生理研究係, 准教授 (70300895) ; 竹林 浩秀 生理学研究所, 分子生理研究系, 助教 (60353439) ; 田中 謙二 生理学研究所, 分子生理研究系, 助手 (30329700)
• Project Period (FY): 2003 – 2007
• Project Status: Completed (Fiscal Year 2007)
• Budget Amount: ¥60,000,000 (Direct Cost: ¥60,000,000); Fiscal Year 2007: ¥12,000,000 (Direct Cost: ¥12,000,000); Fiscal Year 2006: ¥12,000,000 (Direct Cost: ¥12,000,000); Fiscal Year 2005: ¥12,000,000 (Direct Cost: ¥12,000,000); Fiscal Year 2004: ¥12,000,000 (Direct Cost: ¥12,000,000); Fiscal Year 2003: ¥12,000,000 (Direct Cost: ¥12,000,000)
• Keywords: アストロサイト / 多様性 / ATP / グルタミン酸 / セルセンサー / 短期記憶 / 長期記憶 / アレキサンダー病 / 凝集体形成 / GFAP / カイニン酸 / けいれん / 転写因子 / 神経発生 / ドメイン構造 / 放射状グリア細胞

Research Abstract

平成19年度はアストロサイトの多様性について検討した。
アストロサイトは各種の刺激に応答して、グルタミン酸やATPを放出するが、一つのアストロサイトから両方とも放出されるのか、またそれぞれを別個に放出するアストロサイトがあるのか知られていない。われわれはバイオセンサー細胞を用いて、一つのアストロサイトから同時にグルタミン酸とATPの放出を検出する系を確立し、上記の疑問に答えた。例数はまだ多くないが、両方の物質とも放出するもの、どちらかだけしか放出しないものがあることが分かった。
また、グルタミン酸依存性ATP放出をするアストロサイトについても検討した。結果、全くATPを放出しないもの、著しく放出するもの、少ししか放出しないものなど、やはり多様性が認められた。

Research Products

• [Journal Article] Human cortical neurons originate from radial glia and neuron-restricted progenitors. (2007)
  • Author(s): Mo Z, Moore AR, Filipovic R, Ogawa Y, Ikenaka K, Antic SD & Zecevic N
  • Journal Title: J Neurosci 27 Pages: 4132-4145
  • Peer Reviewed
• [Journal Article] Murine model of Alexander disease : analysis of GFAP aggregate formation and its pathological significance. (2007)
  • Author(s): Tanaka KF, Takebayashi H, Ymazaki Y, Ono K, Naruse M, Iwasato T, Itohara S, Kato H, Ikenaka K
  • Journal Title: Glia 55 Pages: 617-631
• [Journal Article] Regulation of filial development by cystatin C. (2007)
  • Author(s): Hasegawa A, Naruse M, Hitoshi S, Iwasaki Y, Takebayashi H, Ikenaka K
  • Journal Title: J Neurochem 100 Pages: 12-22
• [Journal Article] Induced expression of cahepsins and cystatin C in a murine model of demyelination. (2007)
  • Author(s): Ma J, Tanaka KF, Yamada G, Ikenaka K
  • Journal Title: Neurochem Res 100 Pages: 311-320
• [Journal Article] Direct evidence that ventral forebrain cells migrate to the cortex and contribute to the generation of cortical myelinating oligdendrosytes. (2006)
  • Author(s): Nakahira E, Kagawa T, Shimizu T, goulding MD, Ikenaka K
  • Journal Title: Dev Biol 291 Pages: 123-131
• [Journal Article] An animal model for late onset chronic demyelination disease caused by failed terminal differentiation of oligodendrocytes. (2006)
  • Author(s): Ma J, Matusmoto M, Tanaka KF, Takebayashi H, Ikenaka K
  • Journal Title: Neuron Glia Biol 2 Pages: 81-91
• [Journal Article] Incolvement of the Olig 2 transcription factor in cholinergic neuron development of the basal forebrain. (2006)
  • Author(s): Furusho M, Ono K, Takebayashi H, Masahira N, Kagawa T, Ikeda K, Ikenaka K
  • Journal Title: Dev Biol 293 Pages: 348-357
• [Journal Article] Olig2-positive progenitors in the embryonic spinal cord give rise to not only to motoneurons and oligodendrocytes, but alos to a subset of astrocytes and ependymal cells. (2006)
  • Author(s): Masahira N, Takenayashi H, Ono k, Watanabe K, Ding L, Furusho M, Ogawa Y, Nabeshima Y, Alvarez-Buylla A, Shimizu K, Ikenaka K
  • Journal Title: Dev Biol 293 Pages: 358-369
• [Journal Article] Fluoro-jade : Neww fluorescent marker of ROcenthal fibers. (2006)
  • Author(s): Tanaka KF, Ochi N, Hayashi T, Ikeda E, Ikenaka K
  • Journal Title: Neurosci Lett 407 Pages: 127-130
• [Journal Article] Electrophysiological abnormality precedes apparent histological demyelination in the central nercous system of mice over-expressing proteolipid protein. (2006)
  • Author(s): TanakaH, Ikenaka K, Isa T
  • Journal Title: J Neurosci Res 84 Pages: 1206-1216
• [Journal Article] Induction of oligodendrocyte progenitors in dorsal forebrain by intraventricular microinjection of FGF-2. (2006)
  • Author(s): Naruse M, Nakahira e, Miyata T, Hitoshi S, Ikenaka K, Bansal R
  • Journal Title: Dev Biol 297 Pages: 262-273
• [Journal Article] Dorsally derived netrin-1 provides an Inhibitory cue and elaborates the "waiting period" for primary sensory axons in the developing spinal cord (2006)
  • Author(s): Watanabe, K., Tamamaki, N., Furuta, T., Ackerman, AL., Ikenaka, K., Ono, K.
  • Journal Title: Development 133 Pages: 1370-1386
• [Journal Article] Gliogenic radial glial cells show heterogeneity in the developing mouse spinal cord. (2005)
  • Author(s): Ogawa, Y., Takebayashi, H., Takahashi, M., Osumi, N., Iwasaki, Y., Ikenaka, K.
  • Journal Title: Dev.Neurosci 27 Pages: 364-377
• [Journal Article] Wnt Signaling Controls the Timing of Oligodendrocyte Development in the Spinal Cord. (2005)
  • Author(s): Shimizu, T., Kagawa, T., Wada, T., Muroyama, Y., Takada, S., Ikenaka, K.
  • Journal Title: Dev Biol. 282 Pages: 397-410
• [Journal Article] Improved preservation of X-gal reaction product for electron microscopy using hydroxypropyl methacrylate. (2005)
  • Author(s): Masahira, N., Ding, L., Takebayashi, H., Shimizu, K., Ikenaka, K., Ono, K.
  • Journal Title: Neurosci.Lett. 374 Pages: 17-20
• [Journal Article] Isolation of cystatin C via Functional Cloning of astrocyte Differentiation Factors. (2004)
  • Author(s): Kumada, T., Hasegawa, A., Iwasaki, Y., Baba, H., Ikenaka, K.
  • Journal Title: Dev.Neurosci. 26 Pages: 68-76
• [Journal Article] Sulfatide is a negative regulator of oligodendrocyte differentiation : development in sulfatide-null mice. (2004)
  • Author(s): Hirahara, Y., Bansal, R., Honke, K., Wada, Y.
  • Journal Title: Glia 45 Pages: 269-277
• [Journal Article] Tetraspanin protein CD9 is a novel paranodal component regulating paranodal junctional formation. (2004)
  • Author(s): Ishibashi, T., Ding, L., Ikenaka, K., Inoue, Y., Miyado, K., Mekada, E., Baba, H.
  • Journal Title: J.Neurosci. 24(1) Pages: 96-102
• [Journal Article] Ranvier絞輪とイオンチャンネル (2004)
  • Author(s): 田中久貴, 池中一裕
  • Journal Title: CLINICAL NEUROSCIENCE Vol.22,No.7 Pages: 770-772
• [Journal Article] Olig2 positive progenitors in the embryonic spinal cord give rise to not only to motoneurons and oligodendrocytes, but also to a subset of astrocytes and ependymal cells.
  • Author(s): Masahira, N., Takebayashi, H., Ono, K., Watanabe, K., Ding, L et al.
  • Journal Title: Dev.Biol (In press)
• [Journal Article] Involvement of the Olig2 transcription factor in cholinergic neuron development of the basal forebrain.
  • Author(s): Furusho, M., Ono, K., Takebayashi, H., Masahira, N., Kagawa, T., Ikeda, K., Ikenaka, K.
  • Journal Title: Dev Biol. (In press)
• [Journal Article] Direct evidence that ventral forebrain cells migrate to the cortex and contribute to the generation of cortical myelinating oligdendrocytes,
  • Author(s): Nakahira, E., Kagawa, T., Shimizu, T., Goulding, MD, Ikenaka, K.
  • Journal Title: Dev Biol (In press)
• [Journal Article] An animal model for late onset chronic demyelination disease caused by failed terminal differentiation of oligodendrocytes.
  • Author(s): Ma, J., Matsumoto, M., Tanaka K.F., Takebayashi H., Ikenaka K.
  • Journal Title: Neuron Glia Biol (In press)
• [Journal Article] Changes in N-Linked Sugar Chain Patterns Induced by Moderate-to-High Expression of the Galactosyltransferase I Gene in a Brain-derived Cell Line, CG4.
  • Author(s): Menon K.N., Ikeda T., Fujimoto I., Narimatsu H., Nakakita S., Hase S., Ikenaka K.
  • Journal Title: J.Neurosci.Res.
• [Publications] Yokoo H., Nobusawa N., Takebayashi H., Ikenaka K., Isoda K., Kamiya M., Sasaki A., Hirato J., Nakazato Y.: "Anti-human Olig2 antibody as a useful immunohistochemical marker of normal oligodendrocytes and gliomas."Am J Pathology. (in press).
• [Publications] Hirahara, Y., Bansal, R., Honke, K., Ikenaka, K., Wada, Y.: "Sulfatide Is A Negative Regulator Of Oligodendrocyte Differentiation : Development In Sulfatide-Null Mice."Glia. 45. 269-277 (2004)
• [Publications] Ishibashi T, Ding, L., Ikenaka, K., Inoue, Y., Miyado, K., Mekada, E., Baba, H: "Tetraspanin protein CD9 is a novel paranodal component regulating paranodal junctional formation."J.Neurosci.. 24(1). 96-102 (2004)
• [Publications] Iwasaki, Y., Hosoya, T., Takebayashi, H., Ogawa, Y., Hotta, Y., Ikenaka, K: "The potential to induce glial differentiation is conserved between Drosophila and mammalian glial cells missing (gcm) genes."Development. 130. 6027-6035 (2003)
• [Publications] Suzuki, R., Arata, S., Nakajo, S., Ikenaka, K., Kikuyama, S., Shioda, S: "Expression of the receptor for pituitary adenylate cyclase-activating polypeptide(PAC-R) in reactive astrocytes"Moi.Brain Res.. 115. 10-20 (2003)
• [Publications] Matsukawa, N., Ikenaka, K., Nanmoku, K., Yuasa, H., Hattori, M., Kawano, M., Nakazawa, H., Fujimori, O., Ueda, R., Ojika, K: "Brain malformations by retroviral vector-mediated gene transfer of hippocampal cholinergic neurostimulation peptide precursor protein into the CNS via embryonic mice ventricles"Dev.Neurosci.. 25. 349-356 (2003)