Structural study of signal transduction by membrane receptors through protein-protein interactions

• Project/Area Number: 15083102
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Kyoto University (2004-2008); Yokohama City University (2003)
• Principal Investigator: SHIRAKAWA Masahiro Kyoto University, 大学院・工学研究科, 教授 (00202119)
• Co-Investigator(Kenkyū-buntansha): 廣明 秀一 横浜市立大学, 大学院・統合理学研究科, 助教授 (10336589) ; 朽尾 豪人 (栃尾 豪人) 横浜市立大学, 大学院・統合理学研究科, 助手 (70336593)
• Project Period (FY): 2003 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥81,700,000 (Direct Cost: ¥81,700,000); Fiscal Year 2008: ¥15,000,000 (Direct Cost: ¥15,000,000); Fiscal Year 2007: ¥15,000,000 (Direct Cost: ¥15,000,000); Fiscal Year 2006: ¥14,600,000 (Direct Cost: ¥14,600,000); Fiscal Year 2005: ¥14,600,000 (Direct Cost: ¥14,600,000); Fiscal Year 2004: ¥15,000,000 (Direct Cost: ¥15,000,000); Fiscal Year 2003: ¥7,500,000 (Direct Cost: ¥7,500,000)
• Keywords: NMR / 結晶構造解析 / 膜たんぱく質 / 免疫 / 膜受容体 / 立体構造 / X線結晶構造解析法 / インターロイキン / 膜蛋白質 / 小胞輸送 / SUMO / G蛋白質共役受容体 / 生理活性ペプチド / 細胞内輸送 / ペロキシソーム / ユビキチン

Research Abstract

本研究期間中に、チミンDNAグリコシラーゼ(TDG)についてSUMO-3化されたTDGの中央領域の立体構造を論文発表し、非共役型のSUMO-1/TDGの中央領域の立体構造、を解析した。またMCAF1のSUMO結合領域(SIM)とSUMO-3の複合体の立体構造をNMRにより決定した。岐阜大学医学研究科の加藤善一郎博士らとの共同研究により、Myd88のTIRドメインの立体構造をNMRによって決定し、さらにシグナル伝達に関与する分子表面残基を解析した。

Research Products

• [Journal Article] Dual-Function Probe to Detect Protease Activity for Fluorescence Measurement and 19F MRI. (2009)
  • Author(s): Mizukami, S., Takikawa, R., Sugihara, F., Shirakawa, M. and Kikuchi, K.
  • Journal Title: Angewandte Chemie International Edition (Published Online)
• [Journal Article] High-resolution multi-dimensional NMR spectroscopy of proteins in human cells. (2009)
  • Author(s): Inomata, K., Ohno, A., Tochio, H., Isogai, S., Tenno, T., Nakase, I., Takeuchi, T., Futaki, S., Ito, Y., Hiroaki, H, and Shirakawa, M.
  • Journal Title: Nature 458 Pages: 106-109
• [Journal Article] Protein structure determination in living cells by in-cell NMR spectroscopy. (2009)
  • Author(s): Sakakibara, D., Sasaki, A., Ikeya, T., Hamatsu, J., Hanashima, T., Mishima, M., Yoshimasu, M., Hayashi, N., Mikawa, T., Walchli, M., Smith, B. O., Shirakawa, M., Guntert, P. and Ito, Y.
  • Journal Title: Nature 458 Pages: 102-105
• [Journal Article] Molecular basis for SUMOylation-dependent regulation of DNA binding activity of Heat Shock Factor 2. (2009)
  • Author(s): Tateishi, Y., Ariyoshi, M., Igarashi, R., Hara, H., Mizuguchi, K., Seto, A., Nakai, A., Kokubo, T., Tochio, H. and Shirakawa, M.
  • Journal Title: Journal of Biological Chemistry 284 Pages: 2435-2447
• [Journal Article] Dual-Function Probe to Detect Protease Activity fo rFluorescence Measurement and 19F MRI (2009)
  • Author(s): Mizukami, S., Takikawa, R., Sugihara, F., Shirakawa, M. and Kikuchi, K.
  • Journal Title: Angewandte Chemie International Edition (Publisbed Online)
  • Peer Reviewed
• [Journal Article] High-resolution multi-dimensional NMR spectroscopy of proteins in human cells (2009)
  • Author(s): Inomata, R., Ohno, A., Tochio, H. Isogai, S., Tenno, T., Maltase, I., Takeuchi, T., Futaki, S., Ito, Y., Hiroaki, H, and Shirakawa
  • Journal Title: Nature 458 Pages: 106-109
  • Peer Reviewed
• [Journal Article] Protein structure determination in living cells by in-cell NMR spectroscopy (2009)
  • Author(s): sakakloara, D., sasakl, A., lkeya, l., Aamatsu, j.,Aanasnlma,T., Mishima, M., Yoshimasu, M., Hayashi, N., Mikawa, T., Walchli. M., Smith. B. O., Shirakawa. M., Guntert. P. and Ito
  • Journal Title: Nature 458 Pages: 102-105
  • Peer Reviewed
• [Journal Article] Molecular basis for SUMOylation-dependent regulation of DNA binding activity of Heat Shock Factor 2 (2009)
  • Author(s): Tateishi, Y., Ariyoshi, H., Igarashi, R., Hara, H., Mizuguchi, K., Seto, A., Nakai, A., Kolcubo, T., Tochio, H. and Shirakawa, M
  • Journal Title: Journal of Biological Chemistry 284 Pages: 2435-2447
  • Peer Reviewed
• [Journal Article] Structure of the SUMO-interacting motif of MBD1-containing chromatin associated factor 1 (MCAF1) bound to SUMO-3. (2008)
  • Author(s): Sekiyama, N., Ikegami, T., Yamane, T., Ikeguchi, M., Uchimura, Y., Baba, D., Ariyoshi, M., Tochio, H., Saitoh, H. and Shirakawa, M.
  • Journal Title: Journal of Biological Chemistry 283 Pages: 35966-35975
• [Journal Article] Hemi-methylated DNA recognition by the SRA protein Np95 via a base flipping mechanism. (2008)
  • Author(s): Arita, K., Ariyoshi, M., Tochio, H., Nakamura, Y. and Shirakawa, M.
  • Journal Title: Nature 455 Pages: 818-821
• [Journal Article] The solution structure of the C-terminal domain of NfeD reveals a novel membrane-anchored OB-fold. (2008)
  • Author(s): Kuwahara, Y., Ohno, A., Morii, T., Yokoyama, H., Matsui, I., Tochio, H., Shirakawa, M., and Hiroaki, H.
  • Journal Title: Protein Science 17 Pages: 1915-1924
• [Journal Article] Expression, purification, and a structural analysis of human IL-18 binding protein : A potent therapeutic molecule for allergy. (2008)
  • Author(s): Kimura, T., Kato, Z., Ohnishi, H., Tochio, H., Shirakawa, M., Kondo, N.
  • Journal Title: Allergology International 4
  • NAID: 10025108541
• [Journal Article] Intracellular protein delivery activity of peptides derived from insulin-like growth factor binding proteins 3 and 5. (2008)
  • Author(s): Goda, N., Tenno, T., Inomata, K., Shirakawa, M., Tanaka, T., Hiroaki, H.
  • Journal Title: Experimental Cell Research 314 Pages: 2352-2361
• [Journal Article] Kinetic and Thermodynamic evidence for flipping of a methyl-CpG binding domain on methylated DNA. (2008)
  • Author(s): Inomata, K., Ohki, I., Tochio, H., Fujiwara, K., Hiroaki, H., Shirakawa, M.
  • Journal Title: Biochemistry 47 Pages: 3266-3271
• [Journal Article] Paramagnetic Relaxation-Based 19F MRI Probe to Detect Protease. (2008)
  • Author(s): Mizukami, S., Takikawa, R., Sugihara, F., Hori, Y., Tochio, H., Waelchli, M., Shirakawa, M., Kikuchi, K.
  • Journal Title: Activity Journal of the American Chemical Society 130 Pages: 794-795
• [Journal Article] Structure of the SUMO-interacting motif of MBD1-containing chromatin associated factor 1 (MCAF1) bound to SUMO-3 (2008)
  • Author(s): Sekiyama, N., Ikegami, T., Yamane, T., Ikeguchi, M., Uchimura, Y., Baba, D., Ariyoshi, M., Tochio, H., Saitoh, H. and Shirakawa, M
  • Journal Title: Journal of Biological Chemistry 283 Pages: 35966-35975
  • Peer Reviewed
• [Journal Article] Hemi-methylated DNA recognition by the SRA protein Np95 via a base flipping mechanism (2008)
  • Author(s): Arita, K., Ariyoshi, M., TOchio, H., Nakamura, Y. and Shirakawa
  • Journal Title: Nature 455 Pages: 818-821
  • Peer Reviewed
• [Journal Article] The solution structure .of the C-terminal domain of NfeD reveals a novel membrane-anchored OB-fold (2008)
  • Author(s): Kuwahara, Y., Ohno, A., Morii, T., Yokoyama, H., Matsui, I., Tochio, H., Shirakawa, M., and Hiroaki, H.
  • Journal Title: Protein Science 17 Pages: 1915-1924
  • Peer Reviewed
• [Journal Article] Expression, purification, and a structural analysis of human IL-18binding protein : A potent therapeutic molecule for allergy (2008)
  • Author(s): Kimura, T., Kato, Z., Ohnishi, H., Tochio, H., Shirakawa, M., Kondo, N.
  • Journal Title: Allergology International 4 Pages: 367-376
  • NAID: 10025108541
  • Peer Reviewed
• [Journal Article] Intracellular protein delivery activity of peptides derived from insulin-like growth factor binding proteins 3 and 5 (2008)
  • Author(s): Goda, Tenno, T., Inomata, K., Shirakawa, M., Tanaka, T., Hiroaki, H.
  • Journal Title: Experimental Cell Research 314 Pages: 2352-2361
  • Peer Reviewed
• [Journal Article] Kinetic and Thermodynamic evidence for flipping of amethyl-CpG binding domain on methylated DNA. (2008)
  • Author(s): Inomata, K., Ohki, I., Tochio, H., Fujiwara, K., Hiroaki, H., Shirakawa, M.
  • Journal Title: Biochemistry 47 Pages: 3216-3271
  • Peer Reviewed
• [Journal Article] Paramagnetic Relaxation-Based 19F MRI Probe to Detect Protease Activity. (2008)
  • Author(s): Mizukami, S., Takikawa, R., Sugihara, F., Hori, Y., Tochio, H., Waelchli, M., Shirakawa, M., Kikuchi, K.
  • Journal Title: Journal of the American Chemical Society 130 Pages: 794-795
  • Peer Reviewed
• [Journal Article] Structural Analysis of Pituitary Adenylate Cyclase-Activating polypeptides Bound to Phospholipid embranes by Magic Angle Spinning Solid-State NMR. (2007)
  • Author(s): Komi, N., Okawa, K., Tateishi, Y., Shirakawa, M., Fujiwara, T., Akutsu, H.
  • Journal Title: Biochimica et Biophysica Acta -Biomembranes 1768 Pages: 3001-3011
• [Journal Article] Fluoroscopic assessment of protein leakage during Xenopus oocytes in-cell NMR experiment by co-injected EGFP. (2007)
  • Author(s): Sakai, T., Tochio, H., Inomata, K., Sasaki, Y., Tenno, T., Tanaka, T., Kokubo, T., Hiroaki, H., Shirakawa, M.
  • Journal Title: Analytical Biochemistry 371 Pages: 247-249
• [Journal Article] Characterization of the sequence specificity of the R1Bm endonuclease domain by structural and biochemical studies. (2007)
  • Author(s): Maita, N., Aoyagi, H., Osanai, M., Shirakawa, M., Fujiwara, H.
  • Journal Title: Nucleic Acid Research 35 Pages: 3918-3927
• [Journal Article] Magnetic resonance-based visualization of gene expression in mammalian cells using a bacterial polyphosphate kinase reporter gene. (2007)
  • Author(s): Ki, S., Sugihara, F., Kasahara, K., Tochio, H., Shirakawa, M. and Kokubo, T.
  • Journal Title: Biotechniques 42 Pages: 209-215
• [Journal Article] LBT/PTD dual tagged vector for purification, cellular protein delivery and visualization in living cells. (2007)
  • Author(s): Goda, N., Tenno, T., Inomata, K., Iwaya, N., Sasaki, Y., Shirakawa, M., Hiroaki, H.
  • Journal Title: Biochemica et Biophysica Acta 1773 Pages: 141-146
• [Journal Article] Distance determination in human ubiquitin by pulse double electron resonance and double quantum coherence ESR methods. (2007)
  • Author(s): Hara, H., Tenno, T. and Shirakawa, M.
  • Journal Title: Journal of Magnetic Resonance 184 Pages: 78-84
• [Journal Article] Fine-tuning of protein domain boundary by minimizing potential coiled coil regions. (2007)
  • Author(s): Iwaya, N., Goda, N., Unzai, S., Fujiwara, K., Tanaka, T., Tomii, K., Tochio, H., Shirakawa, M. and Hiroaki, H.
  • Journal Title: Journal of biomolecular NMR 37 Pages: 53-63
• [Journal Article] Structural Analysis of Pituitary Adenylate Cyclase-Activating polypeptides Bound to Phospholipid embranes by Magic Angle Spinning Solid-state NMR. (2007)
  • Author(s): Komi, N., Okawa, K., Tateishi, Y., Shirakawa, M., Fujiwara, T., Akutsu, H.
  • Journal Title: Biochimica et Biophysica Acta-Biomembranes 1768 Pages: 3001-3011
  • Peer Reviewed
• [Journal Article] Fluoroscopic assessment of protein leakage during Xenopusoocytes in-cell NMR experiment by co-injected EGFP. (2007)
  • Author(s): Sakai, T., Tochio, H., Inomata, K., Sasaki, Y., Tenno, T., Tanaka, T., Kokubo, T., Hiroaki, H., Shirakawa, M.
  • Journal Title: Analytical Biochemistry 371 Pages: 247-249
  • Peer Reviewed
• [Journal Article] Characterization of the sequence specificity of the R1Bm endonuclease domain by structural and biochemical studies. (2007)
  • Author(s): Maita, N., Aoyagi, H., Osanai, M., Shirakawa, M., Fujiwara, H.,
  • Journal Title: Nucleic Acid Research 35 Pages: 3918-3927
  • Peer Reviewed
• [Journal Article] Distance determination in human ubiquitin by pulse double electron resonance and double quantum coherence ESR methods. (2007)
  • Author(s): Hara, H., Tenno, T., Shirakawa, M.
  • Journal Title: Journal of Magnetic Resonance 184 Pages: 78-84
• [Journal Article] Fine-tuning of protein domain boundary by minimizing potential coiled coil regions. (2007)
  • Author(s): Iwaya, N., Goda, N., Unzai, S., Fujiwara, K., Tanaka, T., Tomii, K., Tochio, H., Shirakawa, M., Hiroaki, H.
  • Journal Title: Journal of biomolecular NMR 37 Pages: 53-63
• [Journal Article] In-Cell NMR Spectroscopy of Proteins inside Xenopus laevis Oocytes. (2006)
  • Author(s): Sakai, T., Tochio, H., Tenno, T., Ito, Y., Kokubo, T., Hiroaki, H. and Shirakawa, M.
  • Journal Title: Journal of biomolecular NMR 36 Pages: 179-188
• [Journal Article] Crystal structure of SUMO-3-modified thymine-DNA glycosylase. (2006)
  • Author(s): Baba, D., Maita; N., Jee, J.-G., Uchimura, Y., Saitoh, H., Sugasawa, K., Hanaoka, F., Tochio, H., Hiroaki, H, Shirakawa, M.
  • Journal Title: Journal of Molecular Biology 359 Pages: 137-147
• [Journal Article] novel magnetic resonance-based method to measure gene expression in living cells. (2006)
  • Author(s): Ki, S., Sugihara, F., Kasahara, K., Tochio, H., Okada-Marubayashi, A., Tomita, S., Morita, M., Ikeguchi, M., Shirakawa, M. and Kokubo, T.
  • Journal Title: Nucleic Acid Research 34
• [Journal Article] The structure of the third intracellular loop of the muscarinic acetylcholine receptor M2 subtype. (2006)
  • Author(s): Ichiyama, S., Oka, Y., Haga, K., Kojima, S., Tateishi, Y., Shirakawa, M., and Haga, T.
  • Journal Title: FEBS Letter 580 Pages: 23-26
• [Journal Article] In-Cell NMR Spectroscopy of Proteins inside Xenopus laevis Oocytes. (2006)
  • Author(s): Sakai, T., Tochio, H., Tenno, T., Ito, Y., Kokubo, T., Hiroaki, H., Shirakawa, M.
  • Journal Title: Journal of biomolecular NMR 36 Pages: 179-188
• [Journal Article] The structure of the third intracellular loop of the muscarinic acetylcholine receptor M2 subtype. (2006)
  • Author(s): Ichiyama, S., Oka, Y., Haga, K., Kojima, S., Tateishi, Y., Sirakawa, M., Haga, T.
  • Journal Title: FEBS Letter. 580 Pages: 23-26
• [Journal Article] Crystal Structure of thymine DNA glycosylase conjugated to SUMO-1. (2005)
  • Author(s): Baba, D., Maita, N., Jee, J.-G., Uchimura, Y., Saitoh, H., Sugasawa, K., Hanaoka, F., Tochio, H., Hiroaki, H., Shirakawa, M.
  • Journal Title: Nature 435 Pages: 979-982
• [Journal Article] The UBL-UBA protein KPC2 is required for degradation of p27 at G1 phase. (2005)
  • Author(s): Hara, T., Kamura, T., Kotoshiba, S., Fujiwara, K., Onoyama, I., Shirakawa, M. and Nakayama, K.-I.
  • Journal Title: Molecular and Cellular Biology 25 Pages: 9292-303
• [Journal Article] Molecular mechanism of a temperature-sensitive phenotype in peroxisomal biogenesis disorder. (2005)
  • Author(s): Hashimoto, K., Kato, Z., Nagase, T., Shimozawa, N., Kuwata, K., Omoya, K., Li, A., Matsukuma, E., Yamamoto, Y., Ohnishi, H., Tochio, H., Shirakawa, M., Suzuki, Y., Wanders, R. J. A., Kondo, N.
  • Journal Title: Pediatric Research 58 Pages: 263-9
• [Journal Article] Structural characterization of the MIT domain from human Vps4b. (2005)
  • Author(s): Takasu, H., Jee, J. G., Ohno, A., Goda, N., Fujiwara, K., Tochio, H., Shirakawa, M., and Hiroaki, H.
  • Journal Title: Biochemical and Biophysical Research Communications 334 Pages: 460-465
• [Journal Article] Structure of the UBA domain of Dsk2p in complex with Ubiquitin : Molecular Determinants for Ubiquitin Recognition (2005)
  • Author(s): Ohno, A., Jee J-G, Fujiwara, K., Tenno, T., Goda, N., Tochio, H., Kobayashi, H., Hiroaki, H., Shirakawa, M.
  • Journal Title: Structure 13 Pages: 521-532
• [Journal Article] Crystal Structure of thymine DNA glycosylase conjugated to SUMO-1 (2005)
  • Author(s): Baba, D., Maita, N., Jee, J.-G., Uchimura Y.Saitoh H., Sugasawa, K., Hanaoka, F..Tochio, H., Hiroaki, H., Shirakawa, M.
  • Journal Title: Nature 435 Pages: 979-982
• [Journal Article] Molecular mechanism of a temperature-sensitive phenotype in peroxisomal biogenesis disorder (2005)
  • Author(s): Hashimoto, K., Kato, Z., Nagase, T., Shimozawa, N., Kuwata K., Omoya K., Li, A., Matsukuma, E., Yamamoto, Y., Ohnishi, H., Tochio, H., Shirakawa, M.
  • Journal Title: Pediatric Research 58 Pages: 263-269
• [Journal Article] Structural characterization of the MIT domain from human Vps4b. (2005)
  • Author(s): Takasu, H., Jee, J.G., Ohno, A., Goda, N., Fujiwara, K., Tochio, H., Shirakawa, M., Hiroaki, H.
  • Journal Title: Biochemical and Biophysical Research Communications 334 Pages: 460-465
• [Journal Article] Backbone (1)H, (13)C, and (15)E.coli nickel binding protein NikA. (2005)
  • Author(s): Rajesh, S., Heddle, J.G., Kurashima-Ito, K., Nietlispach, D., Shirakawa, M., Tame, J.R., Ito, Y
  • Journal Title: J Biomolecular NMR 32 Pages: 117-117
• [Journal Article] Structure of the N-terminal domain of PEX1 AAA-ATPase : characterization of a putative adaptor-binding domain (2004)
  • Author(s): Shiozawa, K.Maita, N., Tomii, K, Seto, A., Goda, N., Akiyama, Y., Shimizu, T., Shirakawa, M., Hiroaki, H.
  • Journal Title: Journal of Biological Chemistry 279 Pages: 50060-50068
• [Journal Article] Crystallographic characterization of the N-terminal domain of PEX1 (2004)
  • Author(s): Shiozawa, K., Maita, N., Tomii, K., Seto, A., Goda, N., Akiyama, Y., Shimizu, T., Shirakawa, M., Hiroaki, H.
  • Journal Title: Acta Crystallography D Biological Crystallography 60 Pages: 2098-2099
• [Journal Article] Structural basis for distinctroles of Lys63- and Lys48-linked polyubiquitin chains. (2004)
  • Author(s): Tenno, T., Fujiwara, K., Tochio, H., Iwai, K., Morita, E.H., Hayashi, H., Murata, S., Hiroaki, H., Sato, M., Tanaka, K., Shirakawa, M.
  • Journal Title: Genes to Cells 9 Pages: 865-875
• [Journal Article] Structure of human MTH1, a Nudix family hydrolase that selectively degrades oxidized purine nucleoside triphosphates (2004)
  • Author(s): Mishima, M., Sakai, Y., Itoh, N., Kamiya, H., Furuichi, M., Takahashi, M., Yamagata, Y., Iwai, S., Nakabeppu, Y., Shirakawa, M.
  • Journal Title: Journal of Biological Chemistry 279 Pages: 33806-33815
• [Journal Article] High-throughput expression vector of isotopically labeled peptides for use in NMR studies (2004)
  • Author(s): Tenno, T., Goda, N., Tateishi, Y., Tochio, H., Mishima, M., Shirakawa, M., Hiroaki, H.
  • Journal Title: Protein Engineering, Design & Selection 17 Pages: 305-314
• [Journal Article] Structural basis for the multiple interactions of the MyD88 TIR domain in TLR4 signaling.
  • Author(s): Ohnishi, H., Tochio, H., Kato, Z., Orii, K., Li, A., Kimura, T., Hiroaki,H., Kondo, N., and Shirakawa, M.
  • Journal Title: Proceedings of the National Academy of Science (in press)
• [Journal Article] Structure of the UBA domain of Dsk2p in complex with Ubiquitin : Molecular Determinants for Ubiquitin Recognition
  • Author(s): Ohno, A., Jee, J-G, Fujiwara, K., Tenno, T., Goda, N., Tochio, H., Kobayashi, H., Hiroaki, H., Shirakawa, M.
  • Journal Title: Structure (印刷中)
• [Presentation] In-cell NMR spectroscopy of proteins in human cells : study of protein interactions and dynamics (2009)
  • Author(s): 白川昌宏
  • Organizer: 特定領域研究「膜インターフェイス」平成20年度公開シンポジウム
  • Place of Presentation: 千里ライフサイエンスセンター
  • Year and Date: 2009-01-23
• [Presentation] In-cell NMRによる細胞質内タンパク質の構造、機能解析 (2008)
  • Author(s): 白川昌宏
  • Organizer: 第31回日本分子生物学会年会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-11
• [Presentation] In-cell NMRによる細胞質内タンパク質の構造,機能解析 (2008)
  • Author(s): 白川昌宏
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大食合同大会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2008-12-11
• [Presentation] In-cell NMRによる細胞内タンパク質の構造、機能の観察 (2008)
  • Author(s): 白川昌宏
  • Organizer: 第8回日本蛋白質科学会
  • Place of Presentation: 東京
  • Year and Date: 2008-06-11
• [Presentation] In-Cell NMR spectroscopy of proteins (2008)
  • Author(s): 白川昌宏
  • Organizer: 日本蛋白質科学会年会
  • Place of Presentation: タワーホール船堀(東京)
  • Year and Date: 2008-06-10
• [Presentation] NMRとMRIを使った細胞内反応の非侵襲計測 (2007)
  • Author(s): 白川昌宏
  • Organizer: 日本化学会第87春季年会
  • Place of Presentation: 大阪
  • Year and Date: 2007-03-29
• [Presentation] SUMO化によるチミンDNAグリコシラーゼ機能調整 (2006)
  • Author(s): 白川昌宏
  • Organizer: 第6回日本蛋白質科学会年会
  • Place of Presentation: 京都
  • Year and Date: 2006-04-26
• [Presentation] SUMOコードの謎を解く (2005)
  • Author(s): 白川昌宏
  • Organizer: 第28回分子生物学会年会
  • Place of Presentation: 福岡
  • Year and Date: 2005-12-10
• [Presentation] 蛋白質修飾による蛋白質の構造変化を捉える-ユビキチン化・SUMO化蛋白質の調製とX線構造解析 (2005)
  • Author(s): 白川昌宏
  • Organizer: 日本結晶学会2005年度年会
  • Place of Presentation: 姫路
  • Year and Date: 2005-12-07
• [Presentation] SUMO化による蛋白質の機能・構造変換の構造学的研究 (2004)
  • Author(s): 白川昌宏
  • Organizer: 第27回日本分子生物学会年会
  • Place of Presentation: 神戸
  • Year and Date: 2004-12-08
• [Presentation] NMR structural biology of protein-nucleic acid recognition (2004)
  • Author(s): 白川昌宏
  • Organizer: 第43回NMR討論会
  • Place of Presentation: 東京
  • Year and Date: 2004-11-11
• [Presentation] 化学修飾による核内制御-DNAメチル化とたんぱく質のユビキチン化 (2004)
  • Author(s): 白川昌宏
  • Organizer: 日本薬学会124年会
  • Place of Presentation: 大阪
  • Year and Date: 2004-03-30
• [Presentation] SUMO化による蛋白質の機能変換の構造学的研究 (2003)
  • Author(s): 白川昌宏
  • Organizer: 第26回日本分子生物学会
  • Place of Presentation: 神戸
  • Year and Date: 2003-12-11
• [Presentation] uaternary structure of polyubiquitin chains (2003)
  • Author(s): 白川昌宏
  • Organizer: 第76回日本生化学会大会
  • Place of Presentation: 横浜
  • Year and Date: 2003-10-17
• [Book] 5.2.1たんぱく質 実験化学講座第5版 日本化学会編8 NMR・ESR (2006)
  • Author(s): 杤尾豪人、白川昌宏
  • Publisher: 丸善株式会社
• [Patent(Industrial Property Rights)] 変異導入MyD88蛋白によるToll様受容体/インターロイキン18シグナルの特異的制御法 (2007)
  • Inventor(s): 近藤直実、加藤善一郎、大西秀典、白川昌宏、杤尾豪人
  • Industrial Property Rights Holder: 財団法人大阪産業振興機構、国立大学法人大阪大学、国立大学法人京都大学
  • Industrial Property Number: 2007-200776
  • Filing Date: 2007-08-01
• [Patent(Industrial Property Rights)] MRI用プローブ (2007)
  • Inventor(s): 菊地和也、水上進、滝川利佳、白川昌宏
  • Industrial Property Rights Holder: 財団法人大阪産業振興機構、国立大学法人大阪大学、国立大学法人京都大学
  • Industrial Property Number: 2007-273956
  • Filing Date: 2007-10-22
• [Patent(Industrial Property Rights)] 変異導入MyD88蛋白によるToll様受容体/インターロイキン18シグナルの特異的制御法 (2007)
  • Inventor(s): 近藤 直実、加藤善一郎、大西 秀典、白川 昌宏、杤尾 豪人
  • Industrial Property Number: 2007-200776
  • Filing Date: 2007-08-01
• [Publications] Auesukaree, C., Homma, T., Tochio, H., Shirakawa, M.Kaneko, Y., Harashima, S.: "Intracellular phosphate serves as a signal for the regulation of the PHO pathway in Saccaromyces cerevisiae"Journal of Biological Chemistry.
• [Publications] Goda, N., Tenno, T., Takasu, H., Hiroaki, H., Shirakawa, M.: "The PRESAT-vector. Asymmetric T-vector for high throughput screening of soluble protein domains for structural proteomics"Protein Science. 13. 652-658 (2004)