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Biospin mediated signaling mechanisms in response to extmcellular stresses

Research Project

Project/Area Number 15087212
Research Category

Grant-in-Aid for Scientific Research on Priority Areas

Allocation TypeSingle-year Grants
Review Section Science and Engineering
Research InstitutionYamagata Promotional Organization for Industrial Technology (2004-2006)
Yamagata Public Corporation For the Development Of Industry, Institute Life Support Technology (2003)

Principal Investigator

YOSHIMURA Tetsuhiko (2005-2006)  Yamagata Promotional Organization for Industrial Thchnology, Director General of Research and Development, 研究開発部, 部長 (70271517)

大矢 博昭 (2003-2004)  財団法人山形県産業技術振興機構, 生物ラジカル研究所, 副所長 (00025389)

Co-Investigator(Kenkyū-buntansha) OGATA Tateaki  Yamagata University, Faculty of Engineering, Professor, 工学部, 教授 (50091830)
YOKOYAMA Hidekatsu  Advanced Industrial Science and Technology Res. Inst. Instrumentation Frontier, Senior Research Scientist, 主任研究員 (10281619)
吉村 哲彦  (財)山形県産業技術振興機構, 生物ラジカル研究所, 副所長 (70271517)
Project Period (FY) 2003 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥26,000,000 (Direct Cost: ¥26,000,000)
Fiscal Year 2006: ¥4,800,000 (Direct Cost: ¥4,800,000)
Fiscal Year 2005: ¥4,600,000 (Direct Cost: ¥4,600,000)
Fiscal Year 2004: ¥7,400,000 (Direct Cost: ¥7,400,000)
Fiscal Year 2003: ¥9,200,000 (Direct Cost: ¥9,200,000)
Keywordsnitric oxide (NO) / nitric oxide synthase (NOS) / lipopolysaccharide (LPS) / Helicobacter pylori / nitrate / gastro-esophageal junction / gastric mucosa / ESR / スピンプローブ法 / 植物 / ストレス応答機構 / オキシデイティブバースト / ジニトロシルジチオラト鉄錯体 / in vivo / サクラマス魚卵 / ストレス / 酸化還元状態 / in vivo ESR / 携帯型 / フリーラジカル / ストレス応答 / 植物用 / 誘導型NO合成酵素 / VCO / ダブルヘルムホルツコイル
Research Abstract

It is now recognized that nitric oxide (NO) is an endogenous mediator of vascular tone, a neurotransmitter in both the peripheral and central nervous systems, and an effector molecule in the immune system. NO is endogenously synthesized from L-arginine by NO synthases (NOSs), which exist in various organs and tissues in mammals. In the gastric mucosa, two types of constitutive NO synthases have been discovered and inducible NO synthase (iNOS), which is expressed in states of inflammation and immune activation, has not been detected in normal rat stomach. Additionally, a non-enzymatic NO production pathway appears to be active in the stomach, in which dietary nitrate is reduced to nitrite by the tongue surface bacteria and the nitrite is then carried into the stomach and reduced to NO under luminal acidic conditions. Endogenous NO, produced through these enzymatic and non-enzymatic pathways, has been demonstrated to be involved not only in the regulation of gastric mucosal blood flow, b … More ut also in the modulation of acid secretion, mucus secretion and gastric motility. In the present study, to elucidate physiological or pathophysiological roles of NO in stomach, we were carried out in vivo study by employing an animal model in which high concentration of NO were generated luminally at the gastro-esophageal junction by administration of nitrite plus acidic ascorbic acid, in vivo study on cross talk between iNOS and cyclooxygenase (COX) in rat gastric mucosa, and in vitro study on the effect of Helicobacter pylori (H. pylori)・lipopolysaccharide (LPS) on the expression of Toll-like receptor (TLR)2 and TLR4 using a mouse normal gastric epithelial cell line (GSM06). Consequently, we obtained following findings : 1) NO generated by nitrite administration on the lumen diffuses into the adjacent gastric tissue to a substantial degree, leading to localized consumption of glutathione in the tissue; 2) the effect of COX activity on iNOS-NO pathway can be important in the regulation of gastric mucosal integrity in inflammation states; 3) H. pylori-LPS stimulation induces TLR2 through TLR4 signaling in normal mouse gastric epithelial GSMO6 cells. This induced TLR2 cooperatively with TLR4 work for iNOS induction by H pylori-LPS simulation. Less

Report

(5 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • 2004 Annual Research Report
  • 2003 Annual Research Report
  • Research Products

    (36 results)

All 2007 2006 2005 2004 2003 Other

All Journal Article (28 results) Book (3 results) Publications (5 results)

  • [Journal Article] Fe-S cluster proteins are intracellular targets for nitric oxide generated luminally at the gastro-oesophageal junction2007

    • Author(s)
      K.Asanuma
    • Journal Title

      Nitric Oxide (印刷中)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Fe-S cluster proteins are intracellular targets for nitric oxide generated luminally at the gastro-oesophageal junction2007

    • Author(s)
      K.Asanuma
    • Journal Title

      Nitric Oxide.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Dinitrosyl iron complex in anti-microbial and cytotoxic action of nitrite", In Radicals for Life : the various forms of nitric oxide2007

    • Author(s)
      T.Yoshimura
    • Journal Title

      Elsevier 10

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] The formation of g=2.49-Species of cytochrome P450 in the rat liver by PCB126 oral administration : identification of heme axial ligands by EPR spectro scopy2006

    • Author(s)
      H.Morita
    • Journal Title

      Biosci. Biotech. Biochem. 70

      Pages: 2974-2981

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] The formation of g=2.49-species of cytochrome P450 in the rat liver by PCB 126 oral administration : identification of heme axial ligands by EPR spectroscopy2006

    • Author(s)
      H.Morita
    • Journal Title

      Biosci. Biotech. Biochem. 70

      Pages: 2974-2981

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] The Formation of g=2.49-Species of Cytochrome P450 in the Rat Liver by PCB126 Oral Administration : Identification of Heme Axial Ligands by EPR Spectroscopy2006

    • Author(s)
      H.Morita
    • Journal Title

      Biosci. Biotech. Biochem 70

      Pages: 2974-2981

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Fe-S cluster proteins are intracellular targets for nitric oxide generated luminally at the gastro-oesophageal junction2006

    • Author(s)
      K.Asanuma
    • Journal Title

      Nitric Oxide (In press)

    • Related Report
      2006 Annual Research Report
  • [Journal Article] EPR imaging to estimate the in vivo intracerebral reducing ability in adolescent rats subjected to neonatal isolation2006

    • Author(s)
      H.Yokoyama
    • Journal Title

      J. Magn. Reson. Imag 23

      Pages: 637-640

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Electrically detected magnetic resonance (EDMR) measurements of bulk silicon carbide (SiC) crystals2006

    • Author(s)
      T.Sato
    • Journal Title

      Chem. Lett. 35

      Pages: 1428-1429

    • Related Report
      2006 Annual Research Report
  • [Journal Article] A new physiologically based pharmacokinetics analysis for in vivo organ disposition of nitroxyl spin probes in mice as measured by multisite detection L-band ESR (MSD-LESR)2006

    • Author(s)
      M.Kamatari
    • Journal Title

      Chem. Lett. 35

      Pages: 1170-1171

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Enhancement of nitroxyl-reducing activity in rats after administration of glutathione studied by an in vivo ESR technique2006

    • Author(s)
      R.Ebina
    • Journal Title

      ITE Lett. 7

      Pages: 488-489

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Diffusion of cytotoxic concentrations of nitric oxide generated luminally at the gatro-esophageal junction of rats2005

    • Author(s)
      K.Asanuma
    • Journal Title

      Gut 54

      Pages: 1072-1075

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Real-time monitoring of reactive oxygen species production during differentiation of human monocytic cell lines (THP-1)2005

    • Author(s)
      S.Kasai
    • Journal Title

      Anal.Chim Acta 549

      Pages: 81-91

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Diffusion of cytotoxic concentrations of nitric oxide generated luminally at the gastro-oesophageal junction of rats2005

    • Author(s)
      K.Asanuma
    • Journal Title

      Gut 54

      Pages: 1072-1077

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Suppression of the pro-apoptotic function of cytochrome c by singlet oxygen via a heme redox state-independent mechanism2005

    • Author(s)
      D.Suto
    • Journal Title

      Biochem.J. 392

      Pages: 399-406

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Sequential changes of nitric oxide levels in the temporal lobe on applying nitric oxide synthase inhibitors and phenobarbital in kainic acid-treated mice2005

    • Author(s)
      N.Kato
    • Journal Title

      Epilep.Res. 65

      Pages: 81-91

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Development of surface-coil-type resonators consisting of an irradiation and receiver coil system for EPR measurements at 700 MHz2005

    • Author(s)
      H.Yokoyama
    • Journal Title

      Appl.Magn.Reson. 29

      Pages: 717-728

    • Related Report
      2005 Annual Research Report
  • [Journal Article] からだの中のラジカルを電子スピン共鳴(ESR)法で見る-ラジカル画像化の方法と最近の進歩2005

    • Author(s)
      吉村哲彦
    • Journal Title

      化学と教育 53

      Pages: 562-565

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Spin trapping of nitric oxide with the iron-dithiocarbamate complex : chemistry and biology2004

    • Author(s)
      T.Yoshimura
    • Journal Title

      Antioxid. Redox Signal. 6

      Pages: 639-647

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] In vivo study on cross-talk between inducible nitric oxide synthase and cyclo oxygenase in rat gastric mucosa : effect of prostaglandin E2 on NO production2004

    • Author(s)
      K.Uno
    • Journal Title

      J. Pharmacol. Exp. Ther. 309

      Pages: 995-1002

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] In vivo study on cross-talk between inducible nitric oxide synthase and cyclooxygenase in rat gastric mucosa : effect of prostaglandin E2 on NO production2004

    • Author(s)
      K.Uno
    • Journal Title

      J. Pharmacol. Exp. Ther 309

      Pages: 995-1002

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] A drastic redox response in plants by environmental stress observed through a novel spin probe2004

    • Author(s)
      M.Aoyama
    • Journal Title

      ITE Letters 5(5)

      Pages: 492-495

    • Related Report
      2004 Annual Research Report
  • [Journal Article] In Vivo Study on Cross-Talk between Inducible Nitric Oxide Synthase and Cyclooxygenase in Rat Gastric Mucosa: Effect of Prostaglandin E2 on NO Production2004

    • Author(s)
      K.Uno
    • Journal Title

      J.Pharmacol.Exp.Ther 309

      Pages: 995-1002

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Spinnokinetic analyses of blood disposition and biliary excretion of nitric oxide (NO)-Fe(II)-N-(Dithiocarboxy)sarcosine complex in rats: BCM-ESR and BEM-SER studies2004

    • Author(s)
      H.Yasui
    • Journal Title

      Free Radic Res. 38

      Pages: 1061-1072

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Spin Trapping of Nitric Oxide with the Iron-Dithiocarbamate Complex: Chemistry and Biology2004

    • Author(s)
      T.Yoshimura
    • Journal Title

      Antioxid.Redox Signal. 6

      Pages: 639-647

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Anti-microbial action against verotoxigenic Escherichia coli O157:H7 by nitric oxide derived from sodium nitrite2004

    • Author(s)
      H.Morita
    • Journal Title

      Biosci.Biotech.Biochem. 68

      Pages: 1027-1034

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Direct detection of nitric oxide and its role in maintaining gastric mucosal integrity following ethanol-induced injury in rats2003

    • Author(s)
      H.Sugata
    • Journal Title

      Free Radic. Res 37

      Pages: 159-169

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Direct detection of nitric oxide and its role in maintaining gastric mucosal integrity following ethanol-induced injury in rats2003

    • Author(s)
      H.Sugata
    • Journal Title

      Free Radic. Res. 37

      Pages: 159-169

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Book] Dinitrosyl iron complex in anti-microbial and cytotoxic action of nitrite", In Radicals for Life : the various forms of nitric oxide2007

    • Author(s)
      T.Yoshimura
    • Total Pages
      10
    • Publisher
      Elsevier
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Book] "Dinitrosyl iron complex in anti-microbial and cytotoxic action of nitrite", In Radicals for Life : the various forms of nitric oxide2007

    • Author(s)
      T.Yoshimura
    • Total Pages
      10
    • Publisher
      Elsevier
    • Related Report
      2006 Annual Research Report
  • [Book] 電子スピン共鳴(ESR, EPR)「生命元素の事典」(桜井弘編)2006

    • Author(s)
      吉村哲彦
    • Total Pages
      6
    • Publisher
      オーム社
    • Related Report
      2005 Annual Research Report
  • [Publications] H.Yokoyama et al.: "Open type EPR spectrometer to measure electron spins of a sample located outside a resonator"Appl.Magn.Reson. 25. 55-63 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] M.Tada, T.Shiraishi, H.Takasina, T.Ohya, T.Ogata: "Direct observation of the redox states of frozen cherry buds by a unique in vivo ESR"Biochem.Biophys.Res.Commn.. 310. 72-77 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] H.Sugata: "Direct detection of nitric oxide and its role in maintaining gastric mucosal intrgrity following ethanol-induced injury in rats"Free Radic.Res. 37. 159-169 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 吉村哲彦(電子スピンサイエンス学会監修): "電子スピンサイエンス&スピンテクノロジー入門、6.2 バイオ電子スピンサイエンス"日本学会事務センター. 10 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 大矢博昭(電子スピンサイエンス学会監修): "電子スピンサイエンス&スピンテクノロジー入門 第8章 ESRに関する学会の紹介"日本学会事務センター. 4 (2003)

    • Related Report
      2003 Annual Research Report

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Published: 2003-04-01   Modified: 2018-03-28  

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