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New Analytical Methods for Molecular Imaging in Single Live Cells and Interfacial Molecular Assemblies

Research Project

Project/Area Number 15105003
Research Category

Grant-in-Aid for Scientific Research (S)

Allocation TypeSingle-year Grants
Research Field Analytical chemistry
Research InstitutionMusashino University (2007)
The University of Tokyo (2003-2006)

Principal Investigator

UMEZAWA Yoshio  Musashino University, Research Institute of Pharmaceutical Sciences, Professor (80011724)

Co-Investigator(Kenkyū-buntansha) OZAWA Takeaki  The University of Tokyo, Graduate School of Science, Professor (40302806)
SATO Moritoshi  The University of Tokyo, Graduate School of Arts and Sciences, Associate Professor (00323501)
NISHINO Tomoaki  The University of Tokyo, Graduate School of Science, Research Associate (80372415)
Project Period (FY) 2003 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥104,910,000 (Direct Cost: ¥80,700,000、Indirect Cost: ¥24,210,000)
Fiscal Year 2007: ¥10,790,000 (Direct Cost: ¥8,300,000、Indirect Cost: ¥2,490,000)
Fiscal Year 2006: ¥16,250,000 (Direct Cost: ¥12,500,000、Indirect Cost: ¥3,750,000)
Fiscal Year 2005: ¥16,250,000 (Direct Cost: ¥12,500,000、Indirect Cost: ¥3,750,000)
Fiscal Year 2004: ¥21,580,000 (Direct Cost: ¥16,600,000、Indirect Cost: ¥4,980,000)
Fiscal Year 2003: ¥40,040,000 (Direct Cost: ¥30,800,000、Indirect Cost: ¥9,240,000)
Keywordsfluorescent indicators / second messenger / fluorescence resonance energy transfer / DNA / Protein phosphorvlation / scanning tunneling microscopy(STM) / molecular tips / 脂質セカンドメッセンジャー / 蛋白質リン酸化
Research Abstract

The purpose of the present research is to develop new analytical methods for molecular imaging.
We have developed optical probes that the intracellular signaling can be monitored in vivo in living cells by genetically encoded intracellular fluorescent and bioluminescent probes or indicators, which include second messengers such as guanosine 3',5'-cyclic monophosphate(cGMP), inositol 1,4,5-trisphosphate(IP3), phosphatidylinositol 3,4,5-trisphosphate(PIP3), and nitric oxide(NO), protein phosphorylations, protein-protein interactions, and protein localizations. An amplifier-coupled fluorescent indicator for NO was developed to visualize physiological nanomolar dynamics of NO in living cells with a detection limit of 0.1 nM. Fluorescent indicators for PIP3 based on FRET allowed localized analysis of PIP3 concentrations. We analyzed the spatio-temporal regulation of the PIP3 production in single living cells. A intracellular fluorescent indicator for another lipid second messenger, diacylgly … More cerol was also reported.
We developed genetically encoded fluorescent indicators for visualizing protein phosphorylations in living cells. As a result of the phosphorylation, FRET was induced between the two fluorescent units. Upon activation of the phosphatases, the phosphorylated substrate domain is dephosphorylated and the FRET signal is decreased.
To monitor protein-protein interactions, a new method was developed by based on split-reporter reconstitution. One of the applications of the split-GFP reconstitution system is a probe for visualizing endogenous mRNA in single living cells. We developed genetically-encoded RNA probes for characterizing localization and dynamics of mtRNA in single living cells. We showed that ND6 mtRNA is localized within mitochondria and concentrated particularly on mtDNA. We demonstrated split-luciferase reconstitution system for a specific protein transported into the intracellular organelles. We developed a genetically encoded bioluminescence indicator for monitoring the release of proteins from the mitochondria in living cells. We also visualized movements of androgen receptor(AR), glucocorticoid receptor(GR) and STAT3 into the nucleus in living cells and mice. These methods allowed high-throughput screening of chemicals with multi-titer plates and imaging of the protein dynamics in living mice.
Nucleobase molecular tips were prepared by chemical modification of underlying metal tips with thiol derivatives of adenine, guanine, cytosine, and uracil and the outmost single nucleobase adsorbate probes intermolecular electron tunneling to or from a sample nucleobase molecule. We found that the electron tunneling between a sample nucleobase and its complementary nucleobase molecular tip was much facilitated compared with its noncomplementary counterpart. Methods of analysis described above have been promised to become key analytical methods for studying chemistry and biology. Less

Report

(6 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • 2005 Annual Research Report
  • 2004 Annual Research Report
  • 2003 Annual Research Report
  • Research Products

    (149 results)

All 2008 2007 2006 2005 2004 2003 Other

All Journal Article (98 results) (of which Peer Reviewed: 47 results) Presentation (21 results) Book (2 results) Patent(Industrial Property Rights) (19 results) (of which Overseas: 14 results) Publications (9 results)

  • [Journal Article] Imaging dynamics of endogenous mitochondrial RNA in single living cells2007

    • Author(s)
      Takeaki Ozawa, et. al.
    • Journal Title

      Nature Methods 4

      Pages: 413-419

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Cyclic Luciferase for Real-Time Sensing of Caspase-3 Activities in Living Mammals2007

    • Author(s)
      Akira Kanno, et. al.
    • Journal Title

      Angew.Chem.Int.Ed. 46

      Pages: 7595-7599

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Epidermal Growth Factor Directs Sex-specific Steroid Signaling through Src Activation2007

    • Author(s)
      Taro Hitosugi, et. al.
    • Journal Title

      J.Biol.Chem. 282

      Pages: 10697-10706

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Bioluminescent Indicator for Determining Protein-Protein Interactions Using Intramolecular Complementation of Split Click Beetle Luciferase2007

    • Author(s)
      Sung Bae Kim, et. al.
    • Journal Title

      Cancer Research 79

      Pages: 4820-4826

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Genetically Encoded Fluorescent Indicators To Visualize Protein Phosphorylation by Extracellular Signal-Regulated Kinase in Single Living Cells2007

    • Author(s)
      Moritoshi Sato, et. al.
    • Journal Title

      Anal.Chem. 79

      Pages: 2570-2575

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Integrated Molecule-Format Bioluminescent Probe for Visualizing Androgenicity of Ligands Based on the Intramolecular Association of Androgen Receptor with Its Recognition Peptide2007

    • Author(s)
      Sung Bae Kim, et. al.
    • Journal Title

      Anal.Chem. 79

      Pages: 1874-1880

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Nongenomic Activity of Ligands in the Association of Androgen Receptor with Src2007

    • Author(s)
      Sung Bae Kim, et. al.
    • Journal Title

      ACS Chem.Biol. 2

      Pages: 484-492

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] A Minimal Peptide Sequence That Targets Fluorescent and Functional Proteins into the Mitochondrial Intermembrane Space2007

    • Author(s)
      Takeaki Ozawa, et. al.
    • Journal Title

      ACS Chem.Biol. 2

      Pages: 176-186

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] A proinflammatory cytokine sensor cell for assaying inflammatory activities of nanoparticles2007

    • Author(s)
      Sung Bae Kim, et. al.
    • Journal Title

      Anal.Biochem. 362

      Pages: 148-150

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Imaging of Selective Nuclear Receptor Modulator-Induced Conformational Changes in the Nuclear Receptor to Allow Interaction with Coactivator and Corepressor Proteins in Living Cells2007

    • Author(s)
      Muhammad Awais, et. al.
    • Journal Title

      ChemBioChem. 8

      Pages: 737-743

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Lipid Raft-Specific Knockdown of Src Family Kinase Activity Inhibits Cell Adhesion and Cell Cycle Progression of Breast Cancer Cells2007

    • Author(s)
      Taro Hitosugi, et. al.
    • Journal Title

      Cancer Research 67

      Pages: 8139-8148

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Optical probes to identify the glucocorticoid receptor ligands in living cells2007

    • Author(s)
      Muhammad Awais, et. al.
    • Journal Title

      Steroids 72

      Pages: 949-954

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] A fluorescent indicator to visualize ligand-induced receptor/coactivator interactions for screening of peroxisome proliferator-activated receptor γ ligands in living cells2007

    • Author(s)
      Muhammad Awais, et. al.
    • Journal Title

      Biosens.Bioelectron. 22

      Pages: 2564-2569

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Imaging dynamics of endogenous mitochondrial RNA in single living cells2007

    • Author(s)
      T. Ozawa, Y. Natori, M. Sato, Y. Umezawa
    • Journal Title

      Nature Methods 4

      Pages: 413-419

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Cyclic Luciferase for Real-Time Sensing of Caspase-3 Activities in Living Mammal2007

    • Author(s)
      A. Kanno, Y. Umezawa, T. Ozawa
    • Journal Title

      Angew. Chem. Int. Ed. 46

      Pages: 7595-7599

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Epidermal Growth Factor Directs Sex-specific Steroid Signaling through Src Activation2007

    • Author(s)
      T. Hitosugi, K. Sasaki, M. Sato, Y. Suzuki, Y. Umezawa
    • Journal Title

      J. Biol. Chem. 282

      Pages: 10697-10706

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Bioluminescent Indicator for Determining Protein-Protein Interactions Using Intramolecular Complementation of Split Click Beetle Luciferase2007

    • Author(s)
      S. B. Kim, Y. Otani, Y. Umezawa, H. Tao
    • Journal Title

      Anal. Chem. 79

      Pages: 4820-4826

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Genetically Encoded Fluorescent Indicators To Visualize Protein Phosphorylation by Extracellular Signal-Regulated Kinase in Single Living Cells2007

    • Author(s)
      M. Sato, Y. Kawai, Y. Umezawa
    • Journal Title

      Anal. Chem. 79

      Pages: 2570-2575

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Integrated Molecule-Format Bioluminescent Probe for Visualizing Androgenicity of Ligands Based on the Intramolecular Association of Androgen Receptor with Its Recognition Peptide2007

    • Author(s)
      S. B. Kim, M, Awais, M. Sato, Y. Umezawa, H. Tao
    • Journal Title

      Anal. Chem. 79

      Pages: 1874-1880

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Nongenomic Activity of Ligands in the Association of Androgen Receptor with Src2007

    • Author(s)
      S. B. Kim, A. Kanno, T. Ozawa, H. Tao, Y. Umezawa
    • Journal Title

      ACS Chem. Biol. 2

      Pages: 484-492

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] A Minimal Peptide Sequence That Targets Fluorescent and Functional Proteins into the Mitochondrial Intermembrane Space2007

    • Author(s)
      T. Ozawa, Y. Sako, Y. Natori, H. Kuroiwa, T. Umezawa
    • Journal Title

      ACS Chem. Biol. 2

      Pages: 176-186

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] A proinflammatory cytokine sensor cell for assaying inslammatory actvities of nanoparticles2007

    • Author(s)
      S. B. Kim, T. Ozawa, H. Tao, Y. Umezawa
    • Journal Title

      Anal. Biochem. 362

      Pages: 148-150

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Imaging of Selective Nuclear Receptor Modulator-Induced Conformational Changes in the Nuclear Receptor to Allow Interaction with Coactivator and Corepressor Proteins in Living Cells2007

    • Author(s)
      M. Awais, M. Sato, Y. Umezawa
    • Journal Title

      Chem.BioChem 8

      Pages: 737-743

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Lipid Raft-Specific Knockdown of Src Family Kinase Activity Inhibits Cell Adhesion and Cell Cycle Progression of Breast Cancer Cells2007

    • Author(s)
      T. Hitosugi, M. Sato, K. Sasaki, Y. Umezawa
    • Journal Title

      Cancer Research 67

      Pages: 8139-8148

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Optical probes to identify the glucocorticoid receptor ligands in living cells2007

    • Author(s)
      M. Awais, M. Sato, Y. Umezawa
    • Journal Title

      Steroids 72

      Pages: 949-954

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] A fluorescent indicator to visualize ligand-induced receptor/coactivator interactions for screening of peroxisome proliferator-activated receptor γ ligands in living cells2007

    • Author(s)
      M. Awais, M. Sato, Y. Umezawa
    • Journal Title

      Biosens. Bioelectron. 22

      Pages: 2564-2569

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Imaging dynamics of endogenous mitochondrial RNA in single living cells2007

    • Author(s)
      Takeaki Ozawa
    • Journal Title

      Nature Methods 4

      Pages: 413-419

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Genetically Encoded Cyclic Luciferase for Rapid Sensing and Real-Time Imaging of Protease Activities in Living Subjects2007

    • Author(s)
      Akira Kanno
    • Journal Title

      Angew.Chem.Int.Ed 46

      Pages: 7595-7599

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Epidermal Growth Factor Directs Sex-specific Steroid Signaling through Src Activation.2007

    • Author(s)
      Taro Hitosugi
    • Journal Title

      J.Biol.Chem. 282

      Pages: 10697-10706

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Lipid raft-specific knockdown of Src family kinase activity inhibits cell adhesion and cell cycle progression of breast cancer cells.2007

    • Author(s)
      Taro Hitosugi
    • Journal Title

      Cancer Research 67

      Pages: 8139-8148

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Genetically Encoded Fluorescent Indicators To Visualize Protein Phosphorylation by Extracellular Signal-Regulated Kinase in Single Living Cells.2007

    • Author(s)
      Moritoshi Sato
    • Journal Title

      Anal.Chem. 79

      Pages: 2570-2575

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Integrated Molecule-Format Bioluminescent Probe for Visualizing Androgenicity of Ligands Based on the Intramolecular Association of Androgen Receptor with Its Recognition Peptide.2007

    • Author(s)
      Sung Bae Kim
    • Journal Title

      Anal.Chem. 79

      Pages: 1874-1880

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Nongenomic Activity of Ligands in the Association of Androgen Receptor with Src.2007

    • Author(s)
      Sung Bae Kim
    • Journal Title

      ACS Chem.Biol. 2

      Pages: 484-492

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A Minimal Peptide Sequence That Targets Fluorescent and Functional Proteins into the Mitochondrial Intermembrane Space.2007

    • Author(s)
      Takeaki Ozawa
    • Journal Title

      ACS Chem.Biol. 2

      Pages: 176-186

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Imaging of Selective Nuclear Receptor Modulator-Induced Conformational Changes in the Nuclear Receptor to Allow Interaction with Coactivator and Corepressor Proteins in Living Cells.2007

    • Author(s)
      Muhammad Awais
    • Journal Title

      Chem Bio Chem, 8

      Pages: 737-743

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Optical probes to identify the glucocorticoid receptor ligands in living cells.2007

    • Author(s)
      Muhammad Awais
    • Journal Title

      Steroids 72

      Pages: 949-954

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A fluorescent indicator to visualize ligand-induced receptor/coactivator interactions for screening of peroxisome proliferator-activated receptor γ ligands in living cells.2007

    • Author(s)
      Muhammad Awais
    • Journal Title

      Biosens.Bioelectron. 22

      Pages: 2564-2569

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A pro-inflammatory cytokine sensor cell for assaying inflammatory activities of nanoparticles.2007

    • Author(s)
      Sung Bae Kim
    • Journal Title

      Anal.Biochem. 362

      Pages: 148-150

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Imaging dynamics of endogenous mitochondrial RNA in single living cells2007

    • Author(s)
      T.Ozawa, Y.Natori, Y.Umezawa
    • Journal Title

      Nature Methods (In press)

    • Related Report
      2006 Annual Research Report
  • [Journal Article] A minimal peptide sequence that targets fluorescent and functional proteins into the mitochondrial intermembrane space.2007

    • Author(s)
      T.Ozawa, Y.Sako, Y.Natori, H.Kuroiwa, T.Kuroiwa, Y.Umezawa
    • Journal Title

      ACS Chemical Biology (In press)

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Imaging diacylglycerol dynamics at organelle membranes2006

    • Author(s)
      Moritoshi Sato, et. al.
    • Journal Title

      Nature Methods 3

      Pages: 797-799

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Complementary base-pair-facilitated electron tunneling for electrically pinpointing complementary nucleobases2006

    • Author(s)
      Takahito Ohshiro, et. al.
    • Journal Title

      Proc.Natl.Acad.Sci., USA 103

      Pages: 10-14

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] A Fluorescent Indicator to Visualize Activities of the Androgen Receptor Ligands in Single Living Cells2006

    • Author(s)
      Muhammad Awais, et. al.
    • Journal Title

      Angew.Chem.Int.Ed. 45

      Pages: 2707-2712

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Cell-Based Indicator to Visualize Picomolar Dynamics of Nitric Oxide Release from Living Cells2006

    • Author(s)
      Moritoshi Sato, et. al.
    • Journal Title

      Anal.Chem. 78

      Pages: 8175-8182

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Genetically Encoded Optical Probe for Detecting Release of Proteins from Mitochondria toward Cytosol in Living Cells and Mammals2006

    • Author(s)
      Akira Kanno, et. al.
    • Journal Title

      Anal.Chem. 78

      Pages: 8076-8081

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Intein-Mediated Reporter Gene Assay for Detecting Protein-Protein Interactions in Living Mammalian Cells2006

    • Author(s)
      Akira Kanno, et. al.
    • Journal Title

      Anal.Chem. 78

      Pages: 556-560

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] A method for determining the activities of cytokines based on the nuclear transport of nuclear factor-kB2006

    • Author(s)
      Sung Bae Kim, et. al.
    • Journal Title

      Anal.Biochem. 359

      Pages: 147-149

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Imaging diacylglycerol dynamics at organelle membranes2006

    • Author(s)
      M. Sato, Y. Ueda, Y. Umezawa
    • Journal Title

      Nature Methods 3

      Pages: 797-799

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Complementary base-pair-facilitated electron tunneling for electrically pinpointing complementary nucleobases2006

    • Author(s)
      T. Ohshiro, Y. Umezawa
    • Journal Title

      Proc. Natl. Acad. Sci., USA 103

      Pages: 10-14

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] A Fluorescent Indicator to Visualize Activities of the Androgen Receptor Ligands in Single Living Cells2006

    • Author(s)
      M. Awais, M. Sato, X. Lee, Y. Umezawa
    • Journal Title

      Angew. Chem. Int. Ed. 45

      Pages: 2707-2712

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Cell-Based Indicator to Visualize Picomolar Dynamics of Nitric Oxide Release from Living Cells2006

    • Author(s)
      M. Sato, T. Nakajima, M. Goto, Y. Umezawa
    • Journal Title

      Anal. Chem. 78

      Pages: 8175-8182

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Genetically Encoded Optical Probe for Detecting Release of Proteins from Mitochondria toward Cytosol in Living Cells and Mammals2006

    • Author(s)
      A. Kanno, T. Ozawa, Y. Umezswa
    • Journal Title

      Anal. Chem. 78

      Pages: 8076-8081

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Intein-Mediated Repoter Gene Assay for Detecting Protein-Protein Interactions in Living Mammalian Cells2006

    • Author(s)
      A. Kanno, T. Ozawa, Y. Umezawa
    • Journal Title

      Anal. Chem. 78

      Pages: 556-560

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] A method for determining the activities of cytokines based on the nuclear transport of nuclear factor-κB2006

    • Author(s)
      S. B. Kim, Y. Natori, Y. Ozawa, Y. Umezawa, H. Tao
    • Journal Title

      Anal. Biochem. 359

      Pages: 147-149

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Complementary base-pair facilitated electron tunneling for electrically retrieving complementary nucleobases.2006

    • Author(s)
      T.Ohshiro, Y.Umezawa
    • Journal Title

      Proc. Natl. Acad. Sci. USA 103

      Pages: 10-14

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Imaging diacylglycerol dynamics at organelle membranes.2006

    • Author(s)
      M.Sato, Y.Ueda, Y.Umezawa
    • Journal Title

      Nature Methods 3

      Pages: 797-799

    • Related Report
      2006 Annual Research Report
  • [Journal Article] A Fluorescent Indicator to Visualize Activities of the Androgen Receptor Ligands in Single Living Cells.2006

    • Author(s)
      M.Awais, M.Sato, X.Lee, Y.Umezawa
    • Journal Title

      Angew. Chem. Int. Ed 45

      Pages: 2707-2712

    • Related Report
      2006 Annual Research Report
  • [Journal Article] A cell-based indicator visualizes picomolar dynamics of nitric oxide release from living cells2006

    • Author(s)
      M.Sato, T.Nakajima, M.Goto, Y.Umezawa
    • Journal Title

      Anal. Chem. 78

      Pages: 8175-8182

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Complementary base-pair facilitated electron tunneling for electrically retrieving complementary nucleobases.2006

    • Author(s)
      T.Ohshiro, Y.Umezawa
    • Journal Title

      Proc.Natl.Acad.Sci.USA 103

      Pages: 10-14

    • Related Report
      2005 Annual Research Report
  • [Journal Article] A fullerene molecular tip can detect localized and rectified electron tunneling within a single fullerene-porphyrin pair2005

    • Author(s)
      Tomoaki Nishino, et. al.
    • Journal Title

      Proc.Natl.Acad.Sci., USA 102

      Pages: 5659-5662

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Imaging the nanomolar range of nitric oxide with an amplifier-coupled fluorescent indicator in living cells2005

    • Author(s)
      Moritoshi Sato, et. al.
    • Journal Title

      Proc.Natl.Acad.Sci., USA 102

      Pages: 14515-14520

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Quantitative Determination of Protein Nuclear Transport Induced by Phosphorylation or by Proteolysis2005

    • Author(s)
      Sung Bae Kim, et. al.
    • Journal Title

      Anal.Chem. 77

      Pages: 6928-6934

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Genetically Encoded Stress Indicator for Noninvasively Imaging Endogenous Corticosterone in Living Mice2005

    • Author(s)
      Sung Bae Kim, et. al.
    • Journal Title

      Anal.Chem. 77

      Pages: 6588-6593

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Locating Inositol 1,4,5-Trisphosphate in the Nucleus and Neuronal Dendrites with Genetically Encoded Fluorescent Indicators2005

    • Author(s)
      Moritoshi Sato, et. al.
    • Journal Title

      Anal.Chem. 77

      Pages: 4751-4758

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] A genetically encoded indicator for assaying bioactive chemicals that induce nuclear transport of glucocorticoid receptor2005

    • Author(s)
      Sung Bae Kim, et. al.
    • Journal Title

      Anal.Biochem. 347

      Pages: 213-220

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] A high-throughput screening of genes that encode proteins transported into the endoplasmic reticulum in mammalian cells2005

    • Author(s)
      Takeaki Ozawa, et. al.
    • Journal Title

      Nucleic Acids Res. 33

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] A fullerene molecular tip can detect localized and rectified electron tunneling within a single fullerene-porphyrin pair2005

    • Author(s)
      T. Nishino, T. Ito, Y. Umezawa
    • Journal Title

      Proc. Natl. Acad. Sci,. USA 102

      Pages: 5659-5662

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Imaging the nanomolar range of nitric oxide with an amplifier-coupled fluorescent indicator in living cells2005

    • Author(s)
      M. Sato, N. Hida, Y. Umezawa
    • Journal Title

      Proc. Natl. Acad. Sci., USA 102

      Pages: 14515-14520

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Quantitative Determination of Protein Nuclear Transport Induced by Phosphorylation or by Proteloysis2005

    • Author(s)
      S. B. Kim, R. Takao, T. Ozawa, Y. Umezawa
    • Journal Title

      Anal. Chem. 77

      Pages: 6928-6934

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Genetically Encoded Stress Indicator for Noninvasively Imaging Endogenous Corticosterone in Living Mice2005

    • Author(s)
      S. B. Kim, T. Ozawa, Y. Umezawa
    • Journal Title

      Anal. Chem. 77

      Pages: 6588-6593

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Locating Inositol 1, 4, 5-Trisphosphate in the Nucleus and Neronal Dendrites with Genetically Encoded Fluorescent Indicators2005

    • Author(s)
      M. Sato, Y. Ueda, M. Shibuya, Y. Umezawa
    • Journal Title

      Anal. Chem. 77

      Pages: 4751-4758

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] A genetically enceded indicator for assaying bioactive chemicals that induce nuclear transport of glucocoticoid receptor2005

    • Author(s)
      S. B. Kim, T. Ozawa, Y. Umezawa
    • Journal Title

      Anal. Biochem. 347

      Pages: 213-220

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] A high-throughput screening of genes that encode proteins transported into the endoplasmic reticulum in mammalian cells2005

    • Author(s)
      T. Ozawa, K. Nishitani, Y. Sako, Y. Umezawa
    • Journal Title

      Nucleic Acids Res 33

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Locating Inositol 1,4,5-trisphosphate in the Nucleus and Neuronal Dendrites with Genetically Encoded Fluorescent Indicators2005

    • Author(s)
      M.Sato, Y.Ueda, M.Shibuya, Y.Umezawa
    • Journal Title

      Anal.Chem. 77

      Pages: 4751-4758

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Imaging the nanomolar range of nitric oxide with an amplifier-coupled fluorescent indicator in living cells2005

    • Author(s)
      M.Sato, N.Hida, M., Y.Umezawa
    • Journal Title

      Proc.Natl.Acad.Sci.USA 102

      Pages: 14515-14520

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Genetically Encoded Stress Indicator for Noninvasively Imaging Endogenous Corticosterone in Living Mice2005

    • Author(s)
      S.B.Kim, T.Ozawa, Y.Umezawa
    • Journal Title

      Anal.Chem. 77

      Pages: 6588-6593

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Quantitative Determination of Protein Nuclear Transport Induced by Phosphorylation or by Proteolysis2005

    • Author(s)
      S.B.Kim, T.Ozawa, Y.Umezawa
    • Journal Title

      Anal.Chem. 77

      Pages: 6928-6934

    • Related Report
      2005 Annual Research Report
  • [Journal Article] A genetically encoded indicator for assaying bioactive chemicals that induce nuclear transport of glucocorticoid receptor2005

    • Author(s)
      S.B.Kim, T.Ozawa, Y.Umezawa
    • Journal Title

      Analytical Biochemistry 347

      Pages: 213-220

    • Related Report
      2005 Annual Research Report
  • [Journal Article] A Fullerene Molecular Tip Can Detect Localized and Rectified Electron Tuuneling within a Single Fuilerene-Porphyrin Pair2005

    • Author(s)
      T.Nishino., T.Ito, Y.Umezawa
    • Journal Title

      Proc.Natl.Acad.Sci. (In press)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] A High-throughput screening of genes that encode proteins transported into the endoplasmic reticulum in mammalian cells2005

    • Author(s)
      T.Ozawa, K.Nishitani, Y.Sako, Y.Umezawa
    • Journal Title

      Nucleic Acids Research 33

    • Related Report
      2004 Annual Research Report
  • [Journal Article] High-throughput sensing and non-invasive imaging of protein nuclear transport by using reconstitution of split Renilla luciferase2004

    • Author(s)
      Sung Bae Kim, et. al.
    • Journal Title

      Proc.Natl.Acad.Sci., USA 101

      Pages: 11542-11547

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Single Color Fluorescent Indicators of Protein Phosphorylation for Multicolor Imaging of Intracellular Signal Flow Dynamics2004

    • Author(s)
      Yasutoshi Kawai, et. al.
    • Journal Title

      Anal.Chem. 76

      Pages: 6144-6149

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] A Genetically Encoded Fluorescent Indicator Capable of Discriminating Estrogen Agonists from Antagonists in Living Cells2004

    • Author(s)
      Muhammad Awais, et. al.
    • Journal Title

      Anal.Chem. 76

      Pages: 2181-2186

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] High-throughput sensing and non-invasive imaging of protein nuclear transport by using rcconstitution of split Renilla luciferase2004

    • Author(s)
      S. B. Kim. T. Ozawa, S. Watanabe, Y. Umezawa
    • Journal Title

      Proc. Natl. Acad. Sci., USA 101

      Pages: 11542-11547

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Single Color Fluorescent Indicators of Protein Phosphorylation for Multicolor Imaging of Intracellular Signal Flow Dynamics2004

    • Author(s)
      Y. Kawai, M. Sato, Y. Umezawa
    • Journal Title

      Anal. Chem. 76

      Pages: 6144-6149

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] A Genetically Encoded Fluorescent Indicator Capable of Discriminating Estrogen Agonists from Antagonists in Living Cells2004

    • Author(s)
      M. Awais, M. Sato, K. Sasaki, Y. Umezawa
    • Journal Title

      Anal. Chem. 76

      Pages: 2181-2186

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] High-throughput sensing and noninvasive imaging of protein unclear transport by using reconstitution of split renilla luciferase2004

    • Author(s)
      S.B.Kim, T.Ozawa, S.Watanabe, Y.Umezawa
    • Journal Title

      Proceedings of National Academy of Sciences of the USA 101

      Pages: 11542-11547

    • Related Report
      2004 Annual Research Report
  • [Journal Article] A Genetically Encoded Fluorescent Indicator Capable of Discrimmating Estrogen Agonists from Antagonists in Living Cells2004

    • Author(s)
      M.Awais, M.Sato, K.Sasaki, Y.Umezawa
    • Journal Title

      Analytical Chemistry 76

      Pages: 2181-2186

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Production of PtdInsP_3 at endomembranes is triggered by receptor endocytosis2003

    • Author(s)
      Moritoshi Sato, et. al.
    • Journal Title

      Nature Cell Biol. 5

      Pages: 1016-1022

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] A genetic approach to identifying mitochondrial proteins2003

    • Author(s)
      Takeaki Ozawa, et. al.
    • Journal Title

      Nature Biotechnol. 21

      Pages: 287-293

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Fluorescent Indicators for Akt/Protein Kinase B and Dynamics of Akt Activity Visualized in Living Cells2003

    • Author(s)
      Kazuki Sasaki, et. al.
    • Journal Title

      J.Biol.Chem. 278

      Pages: 30945-30951

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Locating a Protein-Protein Interaction in Living Cells via Split Renilla Luciferase Complementation2003

    • Author(s)
      Asami Kaihara, et. al.
    • Journal Title

      Anal.Chem. 75

      Pages: 4176-4181

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Selective observation of hydroxy and carboxylate moieties by scanning tunneling microscopy using chemically modified tips with differing extent of hydrogen bond acidity or basicity2003

    • Author(s)
      Tomoaki Nishino, et. al.
    • Journal Title

      J.Electroanal.Chem. 550-551

      Pages: 125-129

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Production of PtdInsP_3 at endomembranes in triggered by receptor endocytosis2003

    • Author(s)
      M. Sato, Y. Ueda, T. Takagi, Y. Umezawa
    • Journal Title

      Nature Cell Biol. 5

      Pages: 1016-1022

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] A genetic approach to identifying mitochondrial proteins2003

    • Author(s)
      T. Ozawa, Y. Sako, M. Sato, T. Kitamura, Y. Umezawa
    • Journal Title

      Nature Biotechnol. 21

      Pages: 287-293

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Fluorescent Indicators for Akt/Protein Kinase B and Dynamics of Akt Activity Visualized in Living Cells2003

    • Author(s)
      K. Sasaki, M. Sato, Y. Umezawa
    • Journal Title

      J. Biol. Chem. 278

      Pages: 30945-30951

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Locating a Protein-Protein Interaction in Living Cells via Split Renilla Luciferase Complementation2003

    • Author(s)
      A. Kaihara, Y. Kawai, M. Sato, T. Ozawa, Y. Umezawa
    • Journal Title

      Anal. Chem. 75

      Pages: 4176-4181

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Selective observation of hydroxy and carboxylate moieties by scanning tunneling microscopy using chemically modified tips with differing extent of hydrogen bond acidity of basicity2003

    • Author(s)
      T. Nishino, T. Ito, Y. Umezawa
    • Journal Title

      J. Electroanal. Chem. 550-551

      Pages: 125-129

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Illuminating Molecular Processes in Linving Cells Molecular tips for Intermolecular Tunneling Microscopy2006

    • Author(s)
      M. Sato, Y. Umezawa
    • Organizer
      The 5th International Forum on Chemistry of Functional Organic Chemicals(IFOC-5)
    • Place of Presentation
      The University of Tokyo, Japan
    • Year and Date
      2006-11-19
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Illuminating molecular processes in living cells2006

    • Author(s)
      Yoshio Umezawa
    • Organizer
      ULP-UT Joint Symposium
    • Place of Presentation
      Tokyo
    • Year and Date
      2006-09-28
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Illuminating molecular processes in living cells2006

    • Author(s)
      Y. Umezawa
    • Organizer
      ULP-UT Joint Symposium
    • Place of Presentation
      The University of Tokyo, Japan
    • Year and Date
      2006-09-28
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Imaging the nanomolar range of nitric oxide with an amplifier-coupled fluorescent indicator in living cells2006

    • Author(s)
      Moritoshi Sato
    • Organizer
      4th International Conference on Biology, Chemistry and Therapeutic Applications of Nitric Oxide
    • Place of Presentation
      California,USA
    • Year and Date
      2006-06-25
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Imaging the nanomolar range of nitric oxide with an amplifier-couplde fluorescent indicator in living cells2006

    • Author(s)
      M. Sato, Y. Umezawa
    • Organizer
      4th International Conference on Biology, Chemistry and Therapeutic Applications of Nitric Oxide
    • Place of Presentation
      Monterey, California, USA
    • Year and Date
      2006-06-25
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Genetically encoded optical indicators for molecular processes in living cells2005

    • Author(s)
      Yoshio Umezawa
    • Organizer
      MB-ITR2005
    • Place of Presentation
      Okazaki
    • Year and Date
      2005-03-04
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Genetically encoded optical indicators for molecular processes in living cells2005

    • Author(s)
      Y. Umezawa
    • Organizer
      MB-ITR2005, Institute for Molecular Science
    • Place of Presentation
      Okazaki, Japan
    • Year and Date
      2005-03-04
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Genetically Encoded Fluorescent and Bioluminescent Indicators for Cellular Signaling Pathways in Live Cells.(Plenary lecture)2004

    • Author(s)
      Yoshio Umezawa
    • Organizer
      ISBC2004
    • Place of Presentation
      Yokohama
    • Year and Date
      2004-08-05
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Genetically Encoded Fluorescent and Bioluminescent Indicators for Cellular Signaling Pathways in Live Cells (Plenary lecture)2004

    • Author(s)
      Y. Umezawa
    • Organizer
      ISBC2004, Yokohama
    • Place of Presentation
      Japan
    • Year and Date
      2004-08-05
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Genetically Encoded Fluorescent Indicators for Visualizing Cellular Signaling in Living Cells2004

    • Author(s)
      Moritoshi Sato
    • Organizer
      FENS Forum 2004
    • Place of Presentation
      Lisbon,Portugal
    • Year and Date
      2004-07-10
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Genetically Encoded Fluorescent Indicators for Visualizing Cellular Singnaling in Living Cells2004

    • Author(s)
      M. Sato, Y. Umezawa
    • Organizer
      FENS Forum 2004, Lisbon
    • Place of Presentation
      Portugal
    • Year and Date
      2004-07-10
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Probing Chemical Processes in Living Cells: Fluorescent Indicators for Imaging Cellular Signaling Pathways2004

    • Author(s)
      Yoshio Umezawa
    • Organizer
      CIS bio international Symposium
    • Place of Presentation
      Avignon,France
    • Year and Date
      2004-04-16
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Probing Chemical Processes in Living Cells : Fluorescent Indicators for Imaging Cellular Signaling pathways2004

    • Author(s)
      Y. Umezawa
    • Organizer
      CIS bio international Symposium, Avignon
    • Place of Presentation
      France
    • Year and Date
      2004-04-16
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Probing Chemical Processes in Living Cells : Fluorescent Indicators for Imaging Cellular Signaling pathways2004

    • Author(s)
      Y. Umezawa
    • Organizer
      227th ACS National Meeting, Anaheim, CA
    • Place of Presentation
      USA
    • Year and Date
      2004-03-31
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Chemical biology of protein splicing : Analytical methods for intracellular molecular imaging with novel split-reporter proteins2004

    • Author(s)
      T. Ozawa
    • Organizer
      NTU-SNU-UT Tri-Department Chemistry Symposium 2004
    • Place of Presentation
      National Taiwan University, Taiwan
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Seeing What was Unseen:New Analytical Methods for Molecular Imaging(Keynote Lecture)2003

    • Author(s)
      Yoshio Umezawa
    • Organizer
      Singapore International Chemical Conference-3(SICC-3)
    • Place of Presentation
      Singapore
    • Year and Date
      2003-12-15
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Seeing What was Unseen : New Analytical Methods for Molecular Imaging (Keynote Lecture)2003

    • Author(s)
      Y. Umezawa
    • Organizer
      Singapore International Chemical Conference-3(SICC-3)
    • Place of Presentation
      Singapore
    • Year and Date
      2003-12-15
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Seeing What Was Unseen ; from interfaces to cellular signaling (Academy guest talk)2003

    • Author(s)
      Y. Umezawa
    • Organizer
      Technical University of Budapest, Budapest
    • Place of Presentation
      Hungary
    • Year and Date
      2003-10-31
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Assay and Screening Methods for Chemicals that Disrupt Cellular Signaling Pathways : Risk Assessment for Potential Endocrine Disruptors (Plenary Lecture) .2003

    • Author(s)
      Y. Umezawa
    • Organizer
      Advanced Analysis-Exploring Biological Systems in Food, Olsztyn
    • Place of Presentation
      Poland
    • Year and Date
      2003-09-03
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Probing Chemical Processes in Living Cells:Applications for Assay and Screening of Chemicals that Promote/Disrupt Cellular Signaling.(Keynote Lecture)2003

    • Author(s)
      Yoshio Umezawa
    • Organizer
      The First International Congress on Bio-Nanointerface(ICBN2003)
    • Place of Presentation
      Tokyo
    • Year and Date
      2003-05-19
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Probing Chemical Processes in Living Cells : Applications for Assay and Screening of Chemicals that Promote/Disrupt Cellular Signaling (Keynote Lecture)2003

    • Author(s)
      Y. Umezawa
    • Organizer
      The First International Congress on Bio-Nanointerface(ICBN2003)
    • Place of Presentation
      Tokyo
    • Year and Date
      2003-05-19
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Book] 「分析化学」2006

    • Author(s)
      梅澤 喜夫
    • Total Pages
      127
    • Publisher
      東京化学同人
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Book] Homing Endonucleases and Inteins

    • Author(s)
      T.Ozawa, Y.Umezawa
    • Publisher
      eds : M.Belfort, V.Derbyshire, B.Stodddard and D.Wood. Springer Verlag (in press)
    • Related Report
      2004 Annual Research Report
  • [Patent(Industrial Property Rights)] 一分子型生物発光可視化プローブ2008

    • Inventor(s)
      梅澤 喜夫・佐藤 守俊・田尾 博明・金 誠培
    • Industrial Property Rights Holder
      東京大学・産業総合研究所
    • Filing Date
      2008-01-15
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Overseas
  • [Patent(Industrial Property Rights)] 一分子型生物発光可視化プローブ2008

    • Inventor(s)
      梅澤 喜夫・佐藤 守俊・田尾 博明・金 誠培
    • Industrial Property Rights Holder
      東京大学・産業総合研究所
    • Filing Date
      2008-01-15
    • Related Report
      2007 Annual Research Report
    • Overseas
  • [Patent(Industrial Property Rights)] キナーゼ阻害性融合タンパク質と医薬組成物2007

    • Inventor(s)
      梅澤 喜夫・佐藤 守俊・一杉 太郎
    • Industrial Property Rights Holder
      梅澤 喜夫
    • Filing Date
      2007-06-08
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Overseas
  • [Patent(Industrial Property Rights)] プロテアーゼ活性化インジケーター2007

    • Inventor(s)
      梅澤 喜夫・小澤 岳昌・菅野 憲
    • Industrial Property Rights Holder
      東京大学・分子科学研究所
    • Industrial Property Number
      2007-124924
    • Filing Date
      2007-05-09
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Patent(Industrial Property Rights)] 疾患細胞ターゲティングプローブ2007

    • Inventor(s)
      梅澤 喜夫, 佐藤 守俊, 鈴木 秀幸
    • Industrial Property Rights Holder
      梅澤 喜夫
    • Industrial Property Number
      2007-093834
    • Filing Date
      2007-03-30
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Patent(Industrial Property Rights)] 分泌制御型プローブ2007

    • Inventor(s)
      梅澤 喜夫, 佐藤 守俊
    • Industrial Property Rights Holder
      東京大学
    • Industrial Property Number
      2007-060230
    • Filing Date
      2007-03-09
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Patent(Industrial Property Rights)] 過酸化水素インディケーター2007

    • Inventor(s)
      梅澤 喜夫, 佐藤 守俊
    • Industrial Property Rights Holder
      梅澤 喜夫
    • Industrial Property Number
      2007-059865
    • Filing Date
      2007-03-09
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Patent(Industrial Property Rights)] タンパク質リン酸化インディケーター2007

    • Inventor(s)
      梅澤 喜夫, 佐藤 守俊
    • Industrial Property Rights Holder
      東京大学
    • Filing Date
      2007-03-06
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Overseas
  • [Patent(Industrial Property Rights)] キナーゼ阻害性融合タンパク質と医薬組成物2007

    • Inventor(s)
      梅澤 喜夫・佐藤 守俊・一杉 太郎
    • Industrial Property Rights Holder
      梅澤喜夫
    • Filing Date
      2007-06-08
    • Related Report
      2007 Annual Research Report
    • Overseas
  • [Patent(Industrial Property Rights)] プロテアーゼ活性化インジケーター2007

    • Inventor(s)
      梅澤 喜夫・小澤 岳昌・菅野 憲
    • Industrial Property Rights Holder
      東京大学・分子科学研究所
    • Filing Date
      2007-05-09
    • Related Report
      2007 Annual Research Report
  • [Patent(Industrial Property Rights)] 真核細胞におけるタンパク質ータンパク質相互作用を検出するためのキットと方法2006

    • Inventor(s)
      梅澤 喜夫, 小澤 岳昌, 菅野 憲
    • Industrial Property Rights Holder
      東京大学
    • Filing Date
      2006-09-29
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Overseas
  • [Patent(Industrial Property Rights)] 一酸化窒素検出・定量用プローブとそれを用いた一酸化窒素の検出・定量方法2005

    • Inventor(s)
      梅澤 喜夫, 佐藤 守俊
    • Industrial Property Rights Holder
      科学技術振興機構
    • Filing Date
      2005-07-05
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Overseas
  • [Patent(Industrial Property Rights)] 一酸化窒素検出用センサー細胞とそれを用いた一酸化窒素の検出・定量方法2005

    • Inventor(s)
      梅澤 喜夫, 佐藤 守俊
    • Industrial Property Rights Holder
      科学技術振興機構
    • Filing Date
      2005-07-05
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Overseas
  • [Patent(Industrial Property Rights)] イノシトール1, 4, 5-三リン酸検出・定量用プローブおよびそれを用いたイノシトール1, 4, 5-三リン酸検出・定量方法2005

    • Inventor(s)
      梅澤 喜夫, 佐藤 守俊
    • Industrial Property Rights Holder
      科学技術振興機構
    • Filing Date
      2005-05-17
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Overseas
  • [Patent(Industrial Property Rights)] 蛋白質核内移行検出用プローブとそれを用いた蛋白質核内移行の検出・定量方法2005

    • Inventor(s)
      梅澤 喜夫, 小澤 岳昌
    • Industrial Property Rights Holder
      科学技術振興機構
    • Filing Date
      2005-03-09
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Overseas
  • [Patent(Industrial Property Rights)] 核内レセプターのアゴニスト・アンタゴニスト検出用プローブとそれを用いた核内レセプターに対するアゴニストおよびアンタゴニストのスクリーニング方法2005

    • Inventor(s)
      梅澤 喜夫, 佐藤 守俊
    • Industrial Property Rights Holder
      科学技術振興機構
    • Filing Date
      2005-02-14
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Overseas
  • [Patent(Industrial Property Rights)] 単色蛍光プローブ2004

    • Inventor(s)
      梅澤 喜夫, 佐藤 守俊
    • Industrial Property Rights Holder
      科学技術振興機構
    • Filing Date
      2004-08-25
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Overseas
  • [Patent(Industrial Property Rights)] 蛋白質相互作用解析用プローブ及びそれを用いた蛋白質相互作用の解析方法2004

    • Inventor(s)
      梅澤 喜夫, 貝原 麻美, 小澤 岳昌, 佐藤 守俊
    • Industrial Property Rights Holder
      科学技術振興機構
    • Filing Date
      2004-05-20
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Overseas
  • [Patent(Industrial Property Rights)] 脂質セカンドメッセンジヤー検出・定量用プローブとそれを用いた脂質セカンドメッセンジャーの検出および定量方法2004

    • Inventor(s)
      梅澤 喜夫, 佐藤 守俊
    • Industrial Property Rights Holder
      科学技術振興機構
    • Filing Date
      2004-03-15
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Overseas
  • [Publications] M.Awais, M.Sato, K.Sasaki, Y.Umezawa: "A Genetically Encoded Fluorescent Indicator Capable of Discriminating Estrogen Agonists from Antagonists in Living Cells"Anal.Chem.. (In press). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] M.Sato, Y.Ueda, T.Takagi, Y.Umezawa: "Production of PtdInsP_3 at endomembranes is triggered by receptor endocytosis"Nature Cell Biology. 5,No.11. 1016-1022 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] A.Kaihara, Y.Kawai, M.Sato, T.Ozawa, Y.Umezawa: "Locating a protein-protein interaction in living cells via split Renilla luciferase complementation"Anal.Chem.. 75,No.16. 4176-4181 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] K.Sasaki, M.Sato, Y.Umezawa: "Fluorescent Indicators for Akt/Protein Kinase B and Dynamics of Akt Activity Visualized in Living Cells"J.Biol.Chem.. 247,No.33. 30945-30951 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] T.Ozawa, Y.Sako, M.Sato, T.Kitamura, Y.Umezawa: "A Genetic Approach to Identifying Mitochondrial Proteins"Nature Biotech. 21. 287-293 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] T.Nishino, T.Ito, Y.Umezawa: "Selective Observation of Hydroxy and Carboxylate Moieties by Scanning Tunneling Microscopy Using Chemically Modified Tips with Differing Extent of I Hydrogen Bond Acidity or Basicity"Journal of Electroanalytical Chemistry. 550-551. 125-129 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] S.B.Kim, T.Ozawa, Y.Umezawa: "A Screening Method for Estrogens Using an Array-Type DNA Glass Slide"Anal Sci. 19. 499-504 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] S.B.Kim, T.Ozawa, Y.Umezawa: "A Screening Method for Estrogens Using an Array-Type DNA Glass Slide"Anal Sci. 19. 499-504 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] H.Aoki, K.Hasegawa, K.Tohda, Y.Umezawa: "Voltammetric detection of inorganic phosphate using ion-channel sensing with self-assembled monolayers of a hydrogen bond-forming receptor"Biosensors and Bioelectronics. 18,No.2-3. 261-267 (2003)

    • Related Report
      2003 Annual Research Report

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Published: 2003-04-01   Modified: 2016-04-21  

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