Development of mutant mice carrying base-substitutions in target genes.

• Project/Area Number: 15200032
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Laboratory animal science
• Research Institution: RIKEN
• Principal Investigator: GONDO Yoichi RIKEN, Population and Quantitative Genomics Team, Deputy Project Director, 個体遺伝情報研究チーム, プロジェクト副ディレクター (40225678)
• Co-Investigator(Kenkyū-buntansha): AIBA Atsu Kobe University, Graduate School of Medicine, Professor, 大学院・医学系研究科, 教授 (20271116) ; UENO Naoto National Institute of Natural Sciences National Institute for Basic Biology, Division of Morphogenesis., Professor, 基礎生物学研究所・形態形成研究部門, 教授 (40221105) ; KOSEKI Haruhiko Niigata University, Graduate School of Medical and Dental Sciences, Professor, 免疫器官形成研究グループ, グループデイレクター (40225446) ; KOMINAMI Ryo Laboratory for Cytokine Signaling, Senior Research Scientist, 大学院医歯学総合研究科, 教授 (40133615) ; NISHIDA Keigo RIKEN, サイトカイン制御研究グループ, 上級研究員 (80360618) ; 阿部 訓也 独立行政法人理化学研究所, 動物変異動態解析技術開発チーム, チームリーダー (40240915) ; 山田 源 国立大学法人熊本大学, 生命資源研究・支援センター, 教授 (80174712) ; 菅原 稔 国立大学法人東北大学, 医学系研究科, 助教授 (20311558)
• Project Period (FY): 2003 – 2006
• Project Status: Completed (Fiscal Year 2006)
• Budget Amount: ¥50,960,000 (Direct Cost: ¥39,200,000、Indirect Cost: ¥11,760,000); Fiscal Year 2006: ¥11,960,000 (Direct Cost: ¥9,200,000、Indirect Cost: ¥2,760,000); Fiscal Year 2005: ¥11,050,000 (Direct Cost: ¥8,500,000、Indirect Cost: ¥2,550,000); Fiscal Year 2004: ¥11,960,000 (Direct Cost: ¥9,200,000、Indirect Cost: ¥2,760,000); Fiscal Year 2003: ¥15,990,000 (Direct Cost: ¥12,300,000、Indirect Cost: ¥3,690,000)
• Keywords: mutagenesis / mouse / reverse genetics / genome function / gene function / induced mutation / ethyl nitrosourea / モデル動物 / 標的遺伝子変異体

Research Abstract

Frozen sperm archive was constructed encompassing 10,000 mice carrying a total of 30,000,000 ENU-induced base substitutions. The genomic DNA archive was also constructed. The objective of this collaborative research is to establish a system by which any researchers may freely and easily obtain mouse strains that carry single-base substitutions in target genes from the large-scale frozen sperm archive. In the 4 years term, members of this research group designed the PCR primers for their target genes and Gondo successfully discovered mutant lines carrying ENU-induced mutations in their target genes. Based upon the progress in this research group, we have made the system available to any researchers within as well as outside of Japan. By the end of the 4 years term, 43 researchers including the members of this research requested a total of 251 target genes, the number of which is 5-fold more than that of the beginning. The number of discovered mutations had already exceeded 400. By using the in vitro fertilizing method, Gondo revived more than 70 mutant lines as live mice. Thus, we have provided expected mutant lines to the users including all the members of this research who designed the PCR primers. It vindicated that not only the system was well established but also we achieved much more than we originally expected. At present, we and users are conducting the analysis of gene functions by using the mutant mouse lines. Some of them have already been published : e.g., model mice for schizophrenia, depression and polydactyly. Summarizing the discovered mutation spectra. 61%, 10% and 29% of the mutations in coding sequences were missesnse, nonsense or splicing anomaly, and synonymous mutatons, respectively. Considering that 1% of mouse genome is coding sequences, the frozen sperm archive consists of 180,000 missense and 30,000 nonsense or splicing anomaly mutations.

Research Products

• [Journal Article] Behavioral phenotypes of Discl messense mutations in mice. (2007)
  • Author(s): S.J.Clapcote
  • Journal Title: Neuron 54 Pages: 383-402
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] A series of ENU-induced single-base substitutions in a long-range cis-element altering Sonic hedgehog expression in the developing mouse limb bud. (2007)
  • Author(s): H.Masuya
  • Journal Title: Genomics 89(2) Pages: 207-214
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Metabotropic glutamate receptor subtype-1 is essential for motor coordination in the adult cerebellum (2007)
  • Author(s): H.Nakao
  • Journal Title: Neurosci. Res 57 Pages: 538-543
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Transposon-tagged mutagenesis in the rat (2007)
  • Author(s): K.Kitada
  • Journal Title: Nature Methods 4(2) Pages: 131-133
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Mammalian Polycomb Scmhl mediates exclusion of Polycomb complexes from the XY body in the pachytene spermatocytes. (2007)
  • Author(s): Y.Takada
  • Journal Title: Development 134(3) Pages: 579-590
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Molecular analysis of coordinated bladder and urogenital organ formation by Hedgehog signaling (2007)
  • Author(s): R.Haraguchi
  • Journal Title: Development 134 Pages: 525-533
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Behavioral phenotype of Disc1 messense mutations in mice. (2007)
  • Author(s): S.J.Clapcote, Lipina, T.V.Millar, J.K..Mackie, S.Christie, S.Ogawa, F.Lerch, J.P., Trimble, K..Uchiyama, M., Sakuraba, Y.Kaneda, H.Shiroishi, T.Houslay, M.D.Henkelman, R.M.Sled, J.G.Gondo, Y.Porteous, D.J., Roder, J.C.
  • Journal Title: Neuron 54 Pages: 383-402
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] A series of ENU-induced single-base substitutions in a long-range cis-element altering Sonic hedgehog expression in the developing mouse limb bud. (2007)
  • Author(s): H.Masuya, Sezutsu, H., Sakuraba, Y., Sagai, T., Hosoya, M., Kaneda, H., Miura, I, Wakana, S., Gondo, Y., Shiroishi, T., Kobayashi, K, Sumiyama, K., Shimizu, A., Nagano, J., Yokoyama, H., Kaneko, S., Sakurai, N., Okagaki, Y., Noda, T., Wakana, S., Gondo, Y., Shiroishi, T.
  • Journal Title: Genomics 89.2 Pages: 207-214
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Mutant Mouse : bona fide biosimulator for the functional annotation of gene and genome networks. (2007)
  • Author(s): Y.Gondo
  • Journal Title: In Springer IIMMS Series "Immunoinformatics" Chapter 10 (Eds by C. Schonbach, S. Ranganathan, V. Brusic)( Springer, New York) (in press)
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Metabotropic glutamate receptor subtype-1 is essential for motor coordination in the adult cerebellum (2007)
  • Author(s): H.Nakao, Nakao, K, Kano, M., Aiba, A
  • Journal Title: Neurosci. Res. 57 Pages: 538-543
  • NAID: 10024413956
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Transposon-tagged mutagenesis in the rat. (2007)
  • Author(s): K.Kitada, Ishishita, S, Tosaka, K, Takahashi, R., Ueda, M., Keng, V.W., Horie, K., Takeda, J.
  • Journal Title: Nat. Methods 4.2 Pages: 131-133
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Zinc is required for FceRI-mediated mast cell activation. (2007)
  • Author(s): K.Kabu, Yamasaki, S., Kamimura, D., Ito, Y., Hasegawa, A., Sato, E., Kitamura, H., Nishida, K., Hirano.T.
  • Journal Title: J. Immunol. 177 Pages: 1296-1305
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Mammalian Polycomb Scmh1 mediates exclusion of Polycomb complexes from the XY body in the pachytene spermatocytes (2007)
  • Author(s): Y.Takada, Isono, K., Shinga, J., Turner, J.M.A, Kitamura, H., Ohara, 0., Watanabe, G, Singh, P.B, Kamijo, T., Jenuwein, T., Burgoyne, P.S, Koseki, H
  • Journal Title: Development 134.3 Pages: 579-590
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Molecular analysis of coordinated bladder and urogenital organ formation by Hedgehog signaling. (2007)
  • Author(s): R.Haraguchi, Motoyama, J., Sasaki, H., Satoh, Y., Miyagawa, S., Nakagata, N., Moon, A., Yamada, G
  • Journal Title: Development 134 Pages: 525-533
  • NAID: 120002468518
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] A series of ENU-induced single-base substitutions in a long-range cis-element altering Sonic hedgehog expression in the developing mouse limb bud. (2007)
  • Author(s): H.Masuya
  • Journal Title: Genomics 89 Pages: 207-214
• [Journal Article] Generation of the mitochondrial bottleneck without the reduction of mtDNA content in mouse female germ cells. (2007)
  • Author(s): L.Cao
  • Journal Title: Nature Genetics (in press)
• [Journal Article] Metabotropic glutamate receptor subtype-1 is essential for motor coordination in the adult cerebellum. (2007)
  • Author(s): H.Nakao
  • Journal Title: Neurosci.Res. (in press)
• [Journal Article] Multi-step lymphomagenesis deduced from DNA changes in thymic lymphomas and atrophic thymuses at various times after γ-irradiation. (2007)
  • Author(s): H Ohi
  • Journal Title: Oncogene (in press)
• [Journal Article] Mammalian Polycomb Scmhl mediates exclusion of Polycomb complexes from the XY body in the pachytene spermatocytes. (2007)
  • Author(s): Y.Takada
  • Journal Title: Development 134 Pages: 579-90
• [Journal Article] An essential role for RasGRPI in mast cell function and IgE-mediated allergic response. (2007)
  • Author(s): L.Liu
  • Journal Title: J.Exp.Med. 204 Pages: 93-103
• [Journal Article] Corrigendum : Cxorf6 is a causative gene for hypospadias. (2007)
  • Author(s): M.Fukami
  • Journal Title: Nat.Genet. 39(1) Pages: 131-131
• [Journal Article] Transposon-tagged mutagenesis in the rat. (2007)
  • Author(s): K.Kitada
  • Journal Title: Nat.Methods. 4(2) Pages: 131-3
• [Journal Article] 変異マウスライブラリー : ENUランダムミュータジェネシスで標的遺伝子に点突然変異をもつマウスを構築する. (2006)
  • Author(s): 権藤洋一
  • Journal Title: 生物の科学 遺伝 別冊 21 Pages: 113-118
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Control of developmental regulators by Polycomb in human embryonic stem cells. (2006)
  • Author(s): T.I.Lee
  • Journal Title: Cell 125 Pages: 301-313
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] WTC deafness Kyoto (dfk) : a rat model for extensive investigations of Kcnq1 functions. (2006)
  • Author(s): H.Gohma
  • Journal Title: Physiol. Genomics 24(3) Pages: 198-206
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Expression of TCRab partly rescues developmental arrest and apoptosis of abT cells in Bcl11b-/- mice (2006)
  • Author(s): J.Inoue
  • Journal Title: J. Immunol. 176 Pages: 5871-5879
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Zinc is required for FceRI-mediated mast cell activation (2006)
  • Author(s): K.Kabu
  • Journal Title: J. Immunol. 177 Pages: 1296-1305
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Distinct roles of Polycomb group gene products between transcriptionally repressed and active domains of Hoxb8 (2006)
  • Author(s): Y.Fujimura
  • Journal Title: Development 133(12) Pages: 2371-2381
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] The selective continued linkage of centromeres from mitosis to interphase in the absence of mammalian separase (2006)
  • Author(s): K.Kumada
  • Journal Title: J. Cell Biol. 172(6) Pages: 835-836
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Altered emotional behavioral responses in mice lacking brain-type fatty acid binding protein gene. (2006)
  • Author(s): Y.Owada
  • Journal Title: European J. Neuroscience 24 Pages: 175-187
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] XGAP, an ArfGAP, is required for polarized localization of PAR proteins and cell polarity in Xenopus gastrulation. (2006)
  • Author(s): J.Hyodo-Miura
  • Journal Title: Dev. Cell 11 Pages: 69-79
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Mutant mouse library : Construction of mice carrying point mutations in target genes by ENU random mutagenesis (in Japanese) (2006)
  • Author(s): Y.Gondo
  • Journal Title: Seibutsu no Kagaku IDEN 21(suppl.) Pages: 113-118
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Control of developmental regulators by Polycomb in human embryonic stem cells (2006)
  • Author(s): T.I.Lee, Jenner, R.G., Boyer, L.A., Guenther, M.G., Levine, S.S., Kumar, R.M., Chevalier, B., Johnstone, S.E., Cole, M.F., Isono, K, Koseki, H., Fuchikami, T., Abe, K., Murray, H.L, Zucker, J.P., Yuan, B., Bell, G.W., Herbolsheimer, E., Hannett, N.M., Sun, K., Odom, D.T., Otte, A.P., Volkert, T.L, Bartel, D.P., Melton, D.A., Gifford, D.K., Jaenisch, R., Young, R.A.
  • Journal Title: Cell 125 Pages: 301-313
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Gene-driven method as a reverse genetics analysis. (in Japanese) (2006)
  • Author(s): Y.Gondo
  • Journal Title: Saibo Kogaku, Suppl. Experimental Protocol Series "Mouse phenotype analysis protocol : from morphology to physiology, Chap 10 (Shujun-sha) Pages: 214-222
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] WTC deafness Kyoto (dfk) : a rat model for extensive investigations of Kcnq1 functions. (2006)
  • Author(s): H.Gohma, Kuramoto, T., Kuwamura, M., Okajima, R., Tanimoto, N., Yamasaki, K, Nakanishi, S., Kitada, K., Makiyama, T., Akao, M., Kita, T., Sasa, M., Serikawa, T.
  • Journal Title: Physiol. Genomics. 24.3 Pages: 198-206
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Expression of TCRab partly rescues developmental arrest and apoptosis of abT cells in Bcl11b-/-micel. (2006)
  • Author(s): J.Inoue, Kanefuji, T., Okazuka, K, Watanabe, H., Mishima Y., Kominami.R.
  • Journal Title: J. Immunology 176 Pages: 5871-5879
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Distinct roles of Polycomb group gene products between transcriptionally repressed and active domains of Hoxb8. (2006)
  • Author(s): Y.Fujimura, Isono, K, Vidal, M., Endoh, M., Kajita, H., Mizutani-Koseki, Y., Takihara, Y., van Lohuizen, M., Otte, A., Jenuwein, T., Deschamps, J., Koseki, H.
  • Journal Title: Development 133.12 Pages: 2371-2381
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] The selective continued linkage of centromeres from mitosis to interphase in the absence of mammalian separase (2006)
  • Author(s): K.Kumada, Yao, R., Kawaguchi, T., Karasawa, M., Hoshikawa, Y., Ichikawa, K., Sugitani, Y., Imoto, I., Inazawa, J., Sugawara, M., Yanagida, M., Noda, T.
  • Journal Title: J. Cell Biol. 172.6 Pages: 835-846
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Altered emotional behavioral responses in mice lacking brain-type fatty acid binding protein gene. (2006)
  • Author(s): Y.Owada, Abdelwahab, S.A., Kitanaka, N., Sakagami, H., Takano, H., Sugitani, Y., Sugawara, M., Kawashima, H., Kiso, Y., Mobarakeh, J.I., Yanai, K, Kaneko, K, Sasaki, H., Kato, H., Saino-Saito, S., Matsumoto, N., Akaike, N., Noda, T., Kondo, H.
  • Journal Title: European J. Neuroscience 24 Pages: 175-187
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] XGAP, an ArfGAP, is required for polarized localization of PAR proteins and cell polarity in Xenopus gastrulation. (2006)
  • Author(s): J.Hyodo-Miura, Yamamoto, T.S., Hyodo, A.C., Iemura, S., Kusakabe, M., Nishida, E., Natsume, T., Ueno, N.
  • Journal Title: Dev. Cell. 11 Pages: 69-79
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Decreased mutant frequency in embryonic brain of DNA polymerase β null mice. (2006)
  • Author(s): N.Niimi
  • Journal Title: Mutagenesis 21 Pages: 55-59
• [Journal Article] Expression of TCRab partly rescues developmental arrest and apoptosis of abT cells in Bcl11b-/-micel. (2006)
  • Author(s): J.Inoue
  • Journal Title: J.Immunology. 176 Pages: 5871-5879
• [Journal Article] Distinct roles of Polycomb group gene products between transcriptionally repressed and active domains of Hoxb8. (2006)
  • Author(s): Y.Jujimura
  • Journal Title: Development 133 Pages: 2371-81
• [Journal Article] Zinc is required for FceRI-mediated mast cell activation. (2006)
  • Author(s): K.Kabu
  • Journal Title: J.Immunol. 177 Pages: 1296-1305
• [Journal Article] The selective continued linkage of centromeres from mitosis to interphase in the absence of mammalian separase. (2006)
  • Author(s): K.Kumada
  • Journal Title: J.Cell Biol. 172(6) Pages: 835-846
• [Journal Article] Altered emotional behavioral responses in mice lacking brain-type fatty acid binding protein gene. (2006)
  • Author(s): Y.Owada
  • Journal Title: European J.Neuroscience 24 Pages: 175-187
• [Journal Article] XGAP,an ArfGAP,is required for polarized localization of PAR proteins and cell polarity in Xenopus gastrulation. (2006)
  • Author(s): J.Hyodo-Miura
  • Journal Title: Dev.Cell 11 Pages: 69-79
• [Journal Article] Monounsaturated fatty acid modification of Wnt protein : its role in Wnt secretion. (2006)
  • Author(s): R.Takada
  • Journal Title: Dev.Cell 11 Pages: 791-801
• [Journal Article] Cxorf6 is a causative gene for hypospadias. (2006)
  • Author(s): M.Fukami
  • Journal Title: Nat.Genet. 38(12) Pages: 1369-71
• [Journal Article] Molecular characterization of ENU mouse mutagenesis and archives. (2005)
  • Author(s): Y.Sakuraba
  • Journal Title: Biochem. Biophys. Res. Commun. 336 Pages: 609-616
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Implementation of the modified-SHIRPA protocol for screening of dominant phenotypes in a large-scale ENU mutagenesis program. (2005)
  • Author(s): H.Masuya
  • Journal Title: Mammalian Genome 16 Pages: 829-837
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Farnesylation of retinal transducin underlies its translocation during light adaptation. (2005)
  • Author(s): H.Kassai
  • Journal Title: Neuron 47 Pages: 529-539
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] p53 prevents maturation of T cell development to the immature CD4-CD8+ stage in Bcl11b-/- mice (2005)
  • Author(s): K.Okazuka
  • Journal Title: Biochem. Biophys. Res. Commun 328 Pages: 545-549
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Fc RI-mediated mast cell degranulation requires calcium-independent microtubule-dependent translocation of granules to the plasma membrane (2005)
  • Author(s): K.Nishida
  • Journal Title: J. Cell Biol. 170 Pages: 115-126
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Hepatocyte, Growth Factor Activator Inhibitor Type 1 (HAI-1) is Required for Branching Morphogenesis in the Chorioallantonic Placenta (2005)
  • Author(s): H.Tanaka
  • Journal Title: Mol. Cell. Biol. 25 Pages: 5687-5698
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Molecular characterization of ENU mouse mutagenesis and archives. (2005)
  • Author(s): Y.Sakuraba, Sezutsu, H., Takahasi, K.R., Tsuchihashi, K., Ichikawa, R., Fujimoto, N., Kaneko, S., Nakai, Y., Uchiyama, M., Goda, N., Motoi, R., Ikeda, A, Karashima, Y., Inoue, M., Kaneda, H., Masuya, H., Minowa, O., Noguchi, H., Toyoda, A., Sakaki, Y., Wakana, S., Noda, T., Shiroishi, T., Gondo, Y.
  • Journal Title: Biochem. Biophys. Res. Commun. 336 Pages: 609-616
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Implementation of the modified-SHIRPA protocol for screening of dominant phenotypes in a large-scale ENU mutagenesis program. (2005)
  • Author(s): H.Masuya, Inoue, M., Wada, Y., Shimizu, A., Nagano, J, Kawai, A., Inoue, A, Kagami, T., Hirayama, T., Yamaga, A., Kaneda, H., Kobayashi, K., Minowa, 0., Miura, I., Gondo, Y., Noda, T., Wakana, S., Shiroishi, T.
  • Journal Title: Mammalian Genome 16 Pages: 829-837
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] p53 prevents maturation of T cell development to the immature CD4-CD8+ stage in Bcl1lb-/-mice. (2005)
  • Author(s): K.Okazuka, Wakabayashi, Y., Kashihara, M., Inoue, J., Sato, T., Yokoyama, M., Aizawa, S., Aizawa, Y., MishimaY., Kominami, R.
  • Journal Title: Biochem. Biophys. Res. Commun. 328 Pages: 545-549
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Fc RI-mediated mast cell degranulation requires calcium-independent microtubule-dependent translocation of granules to the plasma membrane. (2005)
  • Author(s): K.Nishida, Yamasaki, S., Ito, Y., Kabu, K., Hattori, K., Tezuka, T., Nishizumi, H., Kitamura, D., Goitsuka, R., Geha, R.S., Yamamoto, T., Yagi, T., Hirano.T.
  • Journal Title: J. Cell Biol. 170 Pages: 115-126
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Hepatocyte Growth Factor Activator Inhibitor Type 1 (HAI-1) is Required for Branching Morphogenesis in the Chorioallantonic Placenta. (2005)
  • Author(s): H.Tanaka, Nagaike.K., Takeda, N., Itoh, H., Kohama, K., Fukushima, T., Miyata, S., Uchiyama, S., Uchinokura, S., Shimomura, T., Miyazawa, K., Kitamura, N., Yamada, G, Kataoka, H.
  • Journal Title: Mol. Cell. Biol. 25 Pages: 5687-5698
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Implementation of the modified-SHIRPA protocol for screening of dominant phenotypes in a large-scale ENU mutagenesis program. (2005)
  • Author(s): Masuya, H
  • Journal Title: Mammalian Geome 16 Pages: 829-837
• [Journal Article] Enamelin (Enam) is essential for amelogenesis : ENU-induced mouse mutants as models for different clinical subtypes of human amelogenesis imperfecta (AI) (2005)
  • Author(s): Masuya, H
  • Journal Title: Human Molecular Genetics 14(5) Pages: 575-583
• [Journal Article] Molecular characterization of ENU mouse mutagenesis and archives. (2005)
  • Author(s): Sakuraba, Y.
  • Journal Title: Biochemical and Biophysical Research Communications 336 Pages: 609-616
• [Journal Article] 特集「アンチ・エイジングを科学する」、ゲノム維持機構の破綻と遺伝性早老症 (2005)
  • Author(s): 菅原 稔
  • Journal Title: 日本抗加齢医学会雑誌 アンチ・エイジング医学 1(2) Pages: 49-58
• [Journal Article] Birth of Mice Produced by Germ Cell Nuclear Transfer. (2005)
  • Author(s): Miki, H.
  • Journal Title: Genesis 41 Pages: 81-86
• [Journal Article] Disclosing hidden transcripts : mouse natural sense-antisense transcripts tend to be poly(A) negative and nuclear-localized. (2005)
  • Author(s): Kiyosawa, K.
  • Journal Title: Genome Res. 15 Pages: 463-474
• [Journal Article] Presence and distribution of neural crest-derived cells in the murine developing thymus and their potential for differentiation. (2005)
  • Author(s): Yamazaki, H.
  • Journal Title: International Immunology 17 Pages: 549-558
• [Journal Article] Fc{epsilon}RI-mediated mast cell degranulation requires calcium-independent microtubule-dependent translocation of granules to the plasma membrane. (2005)
  • Author(s): Nishida, K.
  • Journal Title: J.Cell Biol. 170 Pages: 115-126
• [Journal Article] The cellular and molecular etiology of the cleft secondary palate in Fgf10 mutant mice. (2005)
  • Author(s): Alappat, SR.
  • Journal Title: Dev Biol. 277 Pages: 102-113
• [Journal Article] Phylogenetic conservation of a limb-specific, cis-acting regulator of Sonic hedgehog (Shh) (2004)
  • Author(s): T.Sagai
  • Journal Title: Mammalian Genome 25 Pages: 23-34
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] A series of maturity onset diabetes of the young, type 2 (MODY2) mouse models generated by a large-scale ENU mutagenesis program. (2004)
  • Author(s): M.Inoue
  • Journal Title: Human Molecular Genetics 13 Pages: 1147-1157
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Develpoment and implementation of a database system to manage a large-scale mouse ENU-mutagenesis program. (2004)
  • Author(s): H.Masuya
  • Journal Title: Mammalian Genome 15 Pages: 404-411
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Enamelin(Enam) is essential for amelogenesis : ENU-induced mouse mutants as models for different clinical subtypes of human amelogenesis imperfecta(AI). (2004)
  • Author(s): H.Masuya
  • Journal Title: Human Molecular Genetics 14 Pages: 575-583
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] ENUマウスミュータジェネシスと新しい逆向遺伝学. (2004)
  • Author(s): 櫻庭喜行
  • Journal Title: 蛋白質核酸酵素 増刊 49(17) Pages: 2642-2648
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Contribution of Asian mouse subspecies Mus musculus molossinus to genomic constitution of strain C57BL/6J, as defined by BAC-end sequence-SNP analysis (2004)
  • Author(s): K.Abe
  • Journal Title: Genome Research 14 Pages: 2439-2447
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Phylogenetic conservation of a limb-specific, cis-acting regulator of Sonic hedgehog (Shh) (2004)
  • Author(s): T.Sagai, Masuya, H., Tamura, M., Shimizu, K., Yada, Y., Wakana, S., Gondo Y., Noda, T., Shiroishi, T.
  • Journal Title: Mammalian Genome 15 Pages: 23-34
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] A series of maturity onset diabetes of the young, type 2 (MODY2) mouse models generated by a large-scale ENU mutagenesis program. (2004)
  • Author(s): M.Inoue, Sakuraba, Y., Motegi, H., Kubota, N., Toki, H., Matsui, J., Toyoda, Y., Miwa, I., Terauchi, Y., Kadowaki, T., Shigeyama, Y., Kasuga, M., Adachi, T., Fujimoto, N., Matsumoto, R., Tsuchihashi, K., Kagami, T., Inoue, A., Kaneda, H., Ishijima, J., Masuya, H., Suzuki, T., Wakana, S., Gondo, Y., Minowa, 0., Shiroishi, T., Noda, T.
  • Journal Title: Human Molecular Genetics 13 Pages: 1147-1157
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Development and implementation of a database system to manage a large-scale mouse ENU-mutagenesis program. (2004)
  • Author(s): H.Masuya, Nakai, Y., Motegi, H., Niinaya, N., Kida, Y., Kaneko, Y., Aritake, H., Suzuki, N., Ishijima, J., Koorikawa, K., Suzuki, T., Aritake, H., Suzuki, N., Ishijima, J., Koorikawa, K., Suzuki, T.Takahashi, K.R., Minowa, 0., Noda, T., Wakana, S., Gondo Y., Shiroishi T.
  • Journal Title: Mammalian Genome 15 Pages: 404-411
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Enamelin (Enam) is essential for amelogenesis : ENU-induced mouse mutants as models for different clinical subtypes of human amelogenesis imperfecta (AI) (2004)
  • Author(s): H.Masuya, Shimizu, K., Sezutsu, H., Sakuraba, Y., Nagano, J., Shimizu, A., Fujimoto, N., Kawai, A., Miura, I., Kaneda, H., Kobayashi, K, Ishijima, J., Maeda, T., Gondo, Y, Noda, T., Wakana S., Shiroishi T.
  • Journal Title: Human Molecular Genetics 14 Pages: 575-583
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] ENU mouse mutagenesis and advanced reverse genetics. (in Japanese) (2004)
  • Author(s): Y.Sakuraba, Gondo, Y.
  • Journal Title: Tanpakushitsu Kakusan Koso 49(17 Suppl) Pages: 2642-2648
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Contribution of Asian mouse subspecies Mus musculus molossinus to genomic constitution of strain C57BL/6J, as defined by BAC-end sequence-SNP analysis (2004)
  • Author(s): K.Abe, Noguchi, H., Tagawa, K., Yuzuriha, M., Toyoda, A., Kojima, T., Ezawa, K., Saitou, N., Hattori, M., Sakaki, Y., Moriwaki, K., Shiroishi, T.
  • Journal Title: Genome Research 14 Pages: 2439-2447
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Large-scale mouse mutagenesis project. (in Japanese) (2004)
  • Author(s): Y.Gondo
  • Journal Title: New Advancement for Genomics and Proteomics, N.T.S. Pages: 1062-1070
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Induced mutations. (in Japanese) (2004)
  • Author(s): M.Inoue, Gondo, Y.
  • Journal Title: Development and Maintenance of Model Animals. L.I.C. Pages: 120-128
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Development and implementation of a database system to manage a large-scale mouse ENU-mutagenesis program. (2004)
  • Author(s): H.Masuya
  • Journal Title: Mamm Genome 15 Pages: 404-411
• [Journal Article] A series of maturity onset diabetes of the young, type 2 (MODY2) mouse models generated by a large-scale ENU mutagenesis program. (2004)
  • Author(s): M, Inoue
  • Journal Title: Human Molecular Genetics 13 Pages: 1147-1157
• [Journal Article] Phylogenetic conservation of a limb-specific, cis-acting regulator of Sonic hedgehog (Shh). (2004)
  • Author(s): T.Sagai
  • Journal Title: Mamm Genome 15 Pages: 23-34
• [Journal Article] Polycomb-group proteins Ring1A/B link ubiquitylation of histone H2A to heritable gene silencing and X inactivation. (2004)
  • Author(s): Napoles M.
  • Journal Title: Dev.Cell. 5 Pages: 663-676
• [Journal Article] Noc2 is essential in normal regulation of exocytosis in endocrine and exocrine cells. (2004)
  • Author(s): Matsumoto M.
  • Journal Title: Proc Natl Acad Sci U S A. 101(22) Pages: 8313-8318
• [Journal Article] Enhanced self-renewal of hematopoietic stem cells mediated by the polycomb gene product Bmi-1 (2004)
  • Author(s): Iwama A.
  • Journal Title: Immunity. 6 Pages: 843-851
• [Journal Article] Involvement of V(D)J recombinase in the generation of intragenic deletions in the Rit1/Bcl11b tumor suppressor gene in gamma-ray-induced thymic lymphomas and in normal thymus of the mouse. (2004)
  • Author(s): Sakata J.
  • Journal Title: Carcinogenesis. 25 Pages: 1069-1075
• [Journal Article] Two distinct methods analyzing chromatin structure using centrifugation and antibodies to modified histone H3 : both provide similar chromatin states of the Rit1/Bcl11b gene. (2004)
  • Author(s): Togashi T.
  • Journal Title: Biochem Biophys Res Commun.Res Commun. 313 Pages: 489-495
• [Journal Article] The D5Mit7 locus on mouse chromosome 5 provides resistance to γ-ray-induced but not N-methyl-N-nitrosourea-induced thymic lymphomas. (2004)
  • Author(s): Kodama Y.
  • Journal Title: Carcinogenesis. 25 Pages: 143-148
• [Journal Article] Development of the mouse external genitalia : unique model of organogenesis. (2004)
  • Author(s): Suzuki, K.
  • Journal Title: Adv Exp Med Biol. 545 Pages: 159-172
• [Journal Article] Bapx1 regulates patterning in the middle ear : Altered regulatory role in the transition from the proximal jaw during vertebrate evolution. (2004)
  • Author(s): Tucker, A.
  • Journal Title: Development 131 Pages: 1235-1245
• [Journal Article] Adenoviral gene transfer of aspartoacylase ameliorates tonic convulsions of spontaneously epileptic rats. (2004)
  • Author(s): Seki T.
  • Journal Title: Neurochem Int. 45(1) Pages: 171-178
• [Journal Article] Rat mutations cvd and hob with cerebellar malformations map to chromosome 2. (2004)
  • Author(s): Kuwamura M.
  • Journal Title: Exp Anim 53(1) Pages: 21-26
• [Journal Article] Gab1 contributes to cytoskeletal reorganization and chemotaxis in response to platelet-derived growth factor. (2004)
  • Author(s): Kallin A.
  • Journal Title: J Biol Chem. 279 Pages: 17897-17904
• [Journal Article] Gene content of the 750 kb critical region for mouse embryonic ectoderm lethal tcl-w5. (2004)
  • Author(s): Abe, K.
  • Journal Title: Mamm.Genome 15 Pages: 265-276
• [Journal Article] Establishment of an efficient BAC transgenesis protocol and its application to functional characterization of the mouse Brachyury locus. (2004)
  • Author(s): Abe, K.
  • Journal Title: Exp.Anim. 53 Pages: 311-320
  • NAID: 10013441566
• [Journal Article] Contribution of Asian mouse subspecies Mus musculus molossinus to genomic constitution of strain C57BL/6J, as defined by BAC end sequence- SNP analysis. (2004)
  • Author(s): Abe, K.
  • Journal Title: Genome Res. 14 Pages: 2439-2447
• [Journal Article] Megasatellite DNA. (2003)
  • Author(s): Y.Gondo
  • Journal Title: Nature Encyclopedia of the Human Genome 3 Pages: 862-865
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Bcl11b is required for differentiation and survival of αβ T lymphocytes. (2003)
  • Author(s): Y.Wakabayashi
  • Journal Title: Nature Immunology 4 Pages: 533-539
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] PKCd is essential for Dishevelled function in a non-canonical Wnt pathway that regulates Xenopus convergent extension movements (2003)
  • Author(s): N.Kinoshita
  • Journal Title: Genes. Dev. 17 Pages: 1663-1676
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Megasatellite DNA. (2003)
  • Author(s): Y.Gondo, Ikeda, J.-E
  • Journal Title: NATURE Encyclopedia of the Human Genome 3 Pages: 862-865
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Bcl11b is required for differentiation and survival of αβ T lymphocytes (2003)
  • Author(s): Y.Wakabayashi, Watanabe, H., Inoue, J., Takeda, N., Sakata, J., Mishima, Y., Hitomi, J., Yamamoto, T., Utsuyama, M., Niwa, O., Aizawa, S., Kominami, R.
  • Journal Title: Nature immunology 4 Pages: 533-539
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] PKCd is essential for Dishevelled function in a non-canonical Wnt pathway that regulates Xenopus convergent extension movements. (2003)
  • Author(s): N.Kinoshita, Iioka, H., Miyakoshi, A., Ueno, N.
  • Journal Title: Genes Dev. 17 Pages: 1663-1676
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Analysis of genome function by mutagenesis (in Japanese) (2003)
  • Author(s): Y.Gondo
  • Journal Title: Genomic Medicine, Kagaku-Dojin Pages: 51-61
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] A novel murine gene, Sickle tail (Skt), linked to the Danforths short tail (Sd) locus, is required for normal development of the intervertebral disc.
  • Author(s): Semba, K.
  • Journal Title: Genetics (In press)
• [Journal Article] Applying whole-genome studies of epigenetic regulation to study human disease.
  • Author(s): Abe, K.
  • Journal Title: Cytogenetic and Genome Research (In press)
• [Journal Article] An optimized beta-gal staining method for simultaneous detection of endogenou gene expression in early mouse embryos.
  • Author(s): Kishigami, S.
  • Journal Title: Genesis (In press)
• [Journal Article] Comparison of gene expression between male and female mouse blastocysts revealed imprinting of the X-linked gene, Rhox5/Pem at preimplantation stages.
  • Author(s): Kobayashi, S.
  • Journal Title: Current Biology (In press)
• [Book] IIMMS Series "Immunoinformatics", : Mutant Mouse : bona fide biosimulator for the functional annotation of gene and genome networks.(Eds by C. Schonbach, S. Ranganathan, V. Brusic)(Springer) (2007)
  • Author(s): Y.Gondo
  • Publisher: Springer(in press)
  • Description: 「研究成果報告書概要(和文)」より
• [Book] Efficient Gene Transduction Technique by Microbubble-Enhanced Sonoporation for embryology.in"Molecular Embryology"edited by Sharpe,P. (2007)
  • Author(s): S.Ohta
  • Publisher: Humana Press.Totowa,New jersey(in press)
• [Book] 細胞工学別冊実験プロトコルシリーズ マウス表現型解析プロトコール : 形態分析から生理機能解析まで 第10章 逆遺伝学的解析法,逆遺伝学的解析法としてのGene-driven法. (2006)
  • Author(s): 権藤洋一
  • Total Pages: 9
  • Publisher: 秀潤社
  • Description: 「研究成果報告書概要(和文)」より
• [Book] 第10章 : 逆遺伝学的解析法,逆遺伝学的解析法としてのGene-driven法.細胞工学別冊実験プロトコルシリーズマウス表現型解析プロトコール 形態分析から生理機能解析まで (2006)
  • Author(s): 権藤 洋一
  • Total Pages: 10
  • Publisher: 秀潤社
• [Book] ゲノミクス・プロテオミクスの新展開 生物情報の解析と応用 : 大規模マウスミュータジェネシスプロジェクト. (2004)
  • Author(s): 権藤洋一
  • Total Pages: 9
  • Publisher: (株)エヌ・ティー・エス
  • Description: 「研究成果報告書概要(和文)」より
• [Book] モデル動物の作製と維持 第4節 誘発変異. (2004)
  • Author(s): 井上麻紀
  • Total Pages: 9
  • Publisher: (株)エル・アイ・シー
  • Description: 「研究成果報告書概要(和文)」より
• [Book] ENUマウスミュータジェネシスと新しい逆向遺伝学「ゲノムネットワーク」(林崎良英監修、鈴木治和、河合純編)蛋白質核酸酵素増刊Vol.49(No.17) (2004)
  • Author(s): 櫻庭喜行
  • Total Pages: 416
  • Publisher: 共立出版
• [Book] マウスの大規模ENUミュータジェネシスの現状と展望「疾患モデル動物--病因解析での役割と限界」別冊医学のあゆみ (2004)
  • Author(s): 権藤洋一
  • Total Pages: 130
  • Publisher: 医歯薬出版
• [Book] 第II部技術解説、第4節誘発変異.モデル動物の作成と維持(森脇和郎、山村研一、米川博通、編集) (2004)
  • Author(s): 井上麻紀
  • Total Pages: 956
  • Publisher: 株)エル・アイ・シー
• [Book] ゲノム創薬 ミュータジェネシスによるゲノム機能解析. (2003)
  • Author(s): 権藤洋一
  • Total Pages: 11
  • Publisher: 化学同人
  • Description: 「研究成果報告書概要(和文)」より
• [Book] 変異マウスラウブラリーENUランダムミュータジェネシスで標的遺伝子に点突然変異をもつマウスを構築する 生物の科学 遺伝 別冊
  • Author(s): 権藤 洋一
  • Publisher: (株)エヌ・ティー・エス(in press)
• [Patent(Industrial Property Rights)] 特定遺伝子に点突然変異をもつ有用なモデルマウス (2004)
  • Inventor(s): 権藤洋一, 瀬筒秀樹, 櫻庭喜行, 高橋亮
  • Industrial Property Rights Holder: 独立行政法人理化学研究所
  • Industrial Property Number: 2004-178832
  • Filing Date: 2004-06-16
• [Publications] 権藤洋一: "モデル動物による遺伝子機能解明"Medical Science Digest. 29(7). 277-280 (2003)
• [Publications] 権藤洋一: "大規模マウスミュータジェネシス"Medical Science Digest. 30(2). 49-50 (2004)
• [Publications] Y.Gondo: "Megasatellite DNA"Nature Encyclopedia of the Human Genome. 3. 862-865 (2003)
• [Publications] T.Sagai: "Phylogenetic conservation of a limb-specific, cis-acting regulator of Sonic hedgehog (Shh)"Mammalian Genome. 15(1). 23-34 (2004)
• [Publications] B.Ohawara: "Role of glypican 4 in the regulation of convergent extension movements during gastrulation in Xenopus laevis."Development. 130. 2129-2138 (2003)
• [Publications] M.Tekeuchi: "Prickle-related gene in vertebrates is essential for gastrulation cell mevements."Curr.Biol.. 13. 674-679 (2003)
• [Publications] T.Ishitani: "he TAKI-NLK mitogen-activated protein kinase cascade functions in the Wnt-5a/Ca (2+) pathway to antagonize Wnt/beta-catenin signaling."Mol Cell Biol.. 23. 131-139 (2003)
• [Publications] M.Suzuki: "Hox proteins functionally cooperate with the GC box-binding protein system through distinct domains."J Biol Chem.. 278. 30148-30156 (2003)
• [Publications] N.Kinoshita: "PKCd is essential for Dishevelled function in a non-canonical Wnt pathway that regulates Xenopus convergent extension movements."Genes Dev.. 17. 1663-1676 (2003)
• [Publications] K.Azumi: "Construction of a cDNA microarray derived from the ascidian Ciona intestinalis."Zoolog Sci.. 20. 1223-1229 (2003)
• [Publications] K.Hotta: "A genome-wide survey of the genes for planar polarity signaling or convergent extension-related genes in Ciona intestinalis and phylogenetic comparisons of evolutionary conserved signaling components."Gene. 317. 165-185 (2003)
• [Publications] F.Carreira-Barbosa: "Prickle 1 regulates cell movements during gastrulation and neuronal migration in zebrafish."Development. 130. 4037-4046 (2003)
• [Publications] N.Ueno: "Planar cell polarity genes and neural tube closure."Birth Defects Res. Part C Embryo Today. 69. 318-324 (2004)
• [Publications] H.Iioka: "Essential role of MARCKS in cortical actin dynamics during gastrulation movements."J Cell Biol.. 164. 169-174 (2004)
• [Publications] 鈴木堅太郎: "マウス外生殖器原基(生殖結節)にみられる伸長制御メカニズム:BMP signalillgの機能解析"細胞工学. 23. 208-209 (2004)
• [Publications] 太田将: "ソノポレーション法によるニワトリ胚への遺伝子導入"細胞工学. 23(1). 94-99 (2004)
• [Publications] M.Yamada: "Silicon Compatible with the Height of Human Vertebral Column."Biological Trace Element Research. 95. 113-121 (2003)
• [Publications] K.Suzuki: "Regulation of outgrowth and apoptosis for the terminal appendage, external genitalia, development by concerted functions of Bmp signaling."Development. 131. 6209-6220 (2003)
• [Publications] S.Ohta: "Microbubble-Enhanced Sonoporation ; Efficient Gene Transduction Technique for Chick Embryos."Genesis. 37. 91-101 (2003)
• [Publications] G.Yamada: "Cellular and molecular mechanisms of development of the external Genitalia."Differentiation. 71. 445-460 (2003)
• [Publications] K.Suzuki: "Developmental biology meets with reproductive engineering ; interdiscipinar science area as a break through."Cell.Mol. Biol.. 49. 653-660 (2003)
• [Publications] I.Oishi: "The receptor tyrosine kinase Ror2 is involved in non-canonical Wnt5a/JNK signalling pathway."Genes Cells. 8. 645-654 (2003)
• [Publications] S.Yamazaki: "Mice with defects in HB-EGF ectodomain shedding show severe developmental abnormalities."J Cell Biol.. 163. 469-475 (2003)
• [Publications] H.Ozaki: "Six1 controls patterning of the mouse otic vesicle."Development. 131. 551-562 (2003)
• [Publications] Y.Nakaoka: "Activation of gp130 Transduces Hypertrophic Signal through Interaction of Scaffolding/Docking Protein Gab1 with Tyrosine Phosphatase SHP2 in Cardiomyocytes"Circulation Research. 93. 221-229 (2003)
• [Publications] S.Yamasaki: "Gads/Grb2-mediated association with LAT is critical for the inhibitory function of Gab2 in T cells."Mol Cell Biol.. 23. 2515-2529 (2003)
• [Publications] S.Yamasaki: "Gab1 is required for EGF receptor signaling and the transformation by activated ErbB2."Oncogene. 13. 1546-1556 (2003)
• [Publications] K.Nishida: "The role of Gab family scaffolding adapter proteins in the signal transduction of cytokine and growth factor receptors."Cancer Science. 94. 1029-1033 (2003)
• [Publications] T.Shirasawa: "Oxygen affinity of hemoglobin regulates O2 consumption, metabolism, and physical activity."J Biol Chem. 278. 5035-5043 (2003)
• [Publications] K.Fukuda: "Mesenchymal expression of Foxl1, a winged helix transcriptional factor, regulates generation and maintenance of gut associated lymphoid organs."Dev.Biol.. 255. 278-289 (2003)
• [Publications] T.Wang: "Forkhead transcription factor Foxf2 (LUN) - deficient mice exhibit abnormal development of secondaroy palate."Dev.Biol.. 259. 83-94 (2003)
• [Publications] R.Yamada: "Cell-autonomous involvement of Mab21/1 is essential for lens placode development."Development. 130. 1759-1770 (2003)
• [Publications] H.Miyagishima: "Dissociation of mammalian Polycomb-group proteins, Ring1B and Rae28/Ph1, from the chromatin correlates with configuration changes of the chromatin in mitotic and meiotic prophase."Histochem Cell Biol.. (in press). (2004)
• [Publications] M.Nakajima: "Abnormal blood Vessel development om moce lacking presenilin-1."Mech.Dev.. 120. 657-667 (2003)
• [Publications] Y.Nakamura: "Accumulation of p27^<KIPI> is associated with BMP2-induced grouth arrest and neuronal differentiation of human neuroblastoma-derived cell lines."BBRC.. 307. 206-213 (2003)
• [Publications] K.Murata: "CD69-null mice protected from arthritis induced with anti-typeII collagen antibodies."International Immunology. 15. 987-992 (2003)
• [Publications] Y.Hoki: "Growth retardation and skin abnormalities of the Recql4-deficient mouse."Human Mol.Genet.. 12. 2293-2299 (2003)
• [Publications] N.Okazaki: "Prediction of the coding sequences of mouse homologues of KIAA gene : III. the complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."DNA Res.. 10(4). 167-180 (2003)
• [Publications] Y.Iwata: "Decomposition of hydroxylamine/water solution with added iron ion."J Hazard Mater.. 104(1-3). 39-49 (2003)
• [Publications] W.de Graaff: "Randomly inserted and targeted Hox/reporter fusions transcriptionally silenced in Polycomb mutants."Proc Natl Acad Sci U S A. 100(23). 13362-13367 (2003)
• [Publications] T.Kaneko: "The mouse YAF2 gene generates two distinct transcripts and is expressed in pre-and postimplantation embryos."Gene. 315. 183-192 (2003)
• [Publications] T.Shin: "Cooperative B7-1/2 (CD80/CD86) and B7-DC costimulation of CD4+ T cells independent of the PD-1 receptor."J Exp Med.. 198(1). 31-38 (2003)
• [Publications] A.Horie: "Generation of a monoclonal antibody against the mouse Sf3b1 (SAP155) gene product for U2 snRNP component of spliceosome."Hybrid Hybridomics.. 22(2). 117-119 (2003)
• [Publications] K.Yamamoto: "The KDEL receptor modulates the endoplasmic reticulum stress response through mitogen-activated protein kinase signaling cascades."J Biol Chem.. 278(36). 34525-34532 (2003)
• [Publications] M.Okazaki: "Increase in plasma fibrinogen and plasminogen activator inhibitor level in diabetic KK and KK-Ay mice."In Vivo.. 17(1). 5-11 (2003)
• [Publications] E.Kondo: "Expression of recombination-activating gene in mature peripheral T cells in Peyer's patch."Int Immunol.. 15(3). 393-402 (2003)
• [Publications] M.Kuwamura: "Rat mutations cvd and hob with cerebellar malformations map to Chromosome 2."Exp.Anim.. (in press). (2004)
• [Publications] E.Nakatsukasa: "Cryopreservation of spermatozoa from closed colonies, and inbred, spontaneous mutant, and transgenic strains of rats."Comp Med.. 53(6). 639-641 (2003)
• [Publications] M.Mori: "A new beige mutant rat ACI/N-Lystbg-Kyo"Exp Anim.. 52(1). 31-36 (2003)
• [Publications] A.S.Cachaco: "Knock-in of integrin β1D affects primmary but not secondary myogenesis in mice."Development. 130. 1659-1671 (2003)
• [Publications] T.Ara: "Impaired colonization of the gonads by primordial germ cells in mice lacking a chemokine, stromal cell-derived factor-1 (SDF-1)."Proceedings of the National Academy of Science of the USA. 100. 5319-5323 (2003)
• [Publications] K.Ohbo: "Identification and characterization of stem cells in prepubertal spermatogenesis in mice."Developmental Biology. 258. 209-225 (2003)
• [Publications] F.Santagati: "Identification of cis-regulatory elements in the mouse Pax9/Nkx2-9 genomic region : Implication for evolutionary conserved synteny."Genetics. 165. 235-242 (2003)
• [Publications] C.Kokubu: "Undulated short-tail deletion mutation in the mouse ablates Pax1 and leads to ectopic activation of neighboring Nkx2-2 in domains that normally express Pax1."Genetics. 165. 299-307 (2003)
• [Publications] Z.Li: "Defective smooth muscle development in qkI-deficient mice."Develop.Growth Diff.. 45. 449-462 (2003)
• [Publications] M.Sugimoto: "Tetraploid embryos rescue the early defects of t^<w5>/t^<w5> mouse embryos."Genesis. 37. 162-171 (2003)
• [Publications] M.Tsuda: "Conserved role of nanos proteins in germ cell development."Science. 301. 1239-1241 (2003)
• [Publications] K.Abe: "Gene content of the 750 kb critical region for mouse embryonic ectoderm lethal tcl-w5."Mamm.Genome. (in press). (2004)
• [Publications] K.Mitsunaga: "Loss of PGC-specific expression of the orphan nuclear receptor ERR- β results in reduction of germ cell number in mouse embryos."Mech.Dev.. (in press). (2004)
• [Publications] S.Yoshida: "neurogenin3 delineates the earliest stages of spermatogenesis in the mouse testis."Dev.Biol.. (in press). (2004)
• [Publications] 権藤洋一: "ゲノム創薬"(株)化学同人. 199 (2003)
• [Publications] 権藤洋一: "別冊・医学のあゆみ疾患モデル動物-病因解析での役割と限界"医歯薬出版(株). 130 (2004)