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The role of p53 in protection of radiation mutagenesis

Research Project

Project/Area Number 15201013
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Risk sciences of radiation/Chemicals
Research InstitutionUniversity of Occupational and Environmental Health, Japan

Principal Investigator

NORIMURA Toshiyuki  Univ.Occup.& Environm.Health, Sch.Med., Professor, 医学部, 教授 (20039530)

Co-Investigator(Kenkyū-buntansha) OOTSUYAMA Akira  Univ.Occup.& Environm.Health, Sch.Med., Associate Professor, 医学部, 助教授 (10194218)
KATO Fumio  Univ.Occup. & Environm.Health, Sch.Med., Assistant Professor, 医学部, 講師 (20309959)
FUJIKAWA Kazuo  Kinki Univ., Faculty of Science & Technology, Professor, 理工学部, 教授 (90247958)
KUNUGITA Naoki  Univ.Occup. & Environm.Health, Sch.Health Science, Associate Professor, 産業保健学部, 助教授 (90178020)
Project Period (FY) 2003 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥28,210,000 (Direct Cost: ¥21,700,000、Indirect Cost: ¥6,510,000)
Fiscal Year 2005: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
Fiscal Year 2004: ¥9,880,000 (Direct Cost: ¥7,600,000、Indirect Cost: ¥2,280,000)
Fiscal Year 2003: ¥10,530,000 (Direct Cost: ¥8,100,000、Indirect Cost: ¥2,430,000)
Keywordsp53 gene / mutation / p53-dependent apoptosis / tissue repair / radiation / dose-rate dependence / genomic instability
Research Abstract

The major risks of low-dose radiation are mutagenesis, teratogenesis and carcinogenesis. All three have been shown (experimentally) to approach zero risk with decreasing dose rate, demonstrating the critical contribution of DNA repair at low dose-rate irradiation. However, DNA repair is not perfect. There must be defense mechanisms other than DNA repair. The influence of p53 on the radiation-induce elevation of T-cell receptor (TCR) variant fractions was examined in splenic T lymphocytes of p53-proficient and -deficient mice.
(1)After acute gamma-irradiation with 3 Gy (@ 61 Gy/h), the TCR variant fraction was higher than the control level. However, when an equal dose of 3 Gy was given but at a lower dose-rate of 0.07 Gy/h, the fraction of TCR variants did not increase at all for p53(+/+) mice, whereas the same dose of 3 Gy remained mutagenic for p53(-/-) mice, which are unable to carry out p53-dependent apoptosis.
(2)Initially, the TCR variant fraction increased rapidly and reached its maximum level at 9 days after irradiation before decreasing gradually. In p53(+/+) and p53(+/-) mice, the TCR variant fraction fell to normal background levels at 16 and 20 weeks of age, respectively. After the long flat period, a significant re-increase in the fraction of TCR variants was found after 72 weeks of age in the irradiated p53(+/+) mice and after 44 weeks of age in the irradiated p53(+/-) mice. This delayed increase in TCR variants after irradiation is due to a reduction in p53-dependent apoptosis.
These results indicate that the p53 gene is indispensable for a threshold effect in the risk of radiation at low doses or dose-rate.

Report

(4 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • 2003 Annual Research Report
  • Research Products

    (17 results)

All 2006 2005 2003 Other

All Journal Article (13 results) Publications (4 results)

  • [Journal Article] The delayed manifestation of T-cell receptor (TCR) variants in X-irradiated mice depends on Trp53 status2006

    • Author(s)
      K.IGARI
    • Journal Title

      Radiation Research (in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] 体細胞における突然変異2006

    • Author(s)
      法村 俊之
    • Journal Title

      エネルギー 39・3

      Pages: 62-63

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] The delayed manifestation of T-cell receptor (TCR) variants in X-irradiated mice depends on Trp53 status.2006

    • Author(s)
      Igari, K., Igari, Y., Okazaki, R., Kato, F., Ootsuyama, A., Norimura, T.
    • Journal Title

      Radiation Research (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Radioadaptive response for protection against radiation-induced teratogenesis2005

    • Author(s)
      R.OKAZAKI
    • Journal Title

      Radiation Research 163・3

      Pages: 266-270

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] Increased radiosensitivity of splenic T lymphocytes in pregnant mice2005

    • Author(s)
      Y.IGARI
    • Journal Title

      International Congress Series 1276

      Pages: 183-184

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary 2004 Annual Research Report
  • [Journal Article] Role of p53 gene in genetic instability induced by ionizing radiation2005

    • Author(s)
      K.IGARI
    • Journal Title

      International Congress Series 1276

      Pages: 262-263

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary 2004 Annual Research Report
  • [Journal Article] Radioadaptive response for protection against radiation-induced teratogenesis.2005

    • Author(s)
      Okazaki, R., Ootsuyama, A., Norimura, T.
    • Journal Title

      Radiation Research 163(3)

      Pages: 266-270

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Increased radiosensitivity of splenic T lymphocytes in pregnant mice.2005

    • Author(s)
      Igari, Y., Igari, K., Kakihara, H., Kato, F., Ootsuyama, A., Norimura, T.
    • Journal Title

      International Congress Series 1276

      Pages: 183-184

    • NAID

      130005443068

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Role of p53 gene in genetic instability induced by ionizing radiation.2005

    • Author(s)
      Igari, K., Igari, Y., Kakihara, H., Kato, F., Ootsuyama, A., Norimura, T.
    • Journal Title

      International Congress Series 1276

      Pages: 262-263

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Radioadaptive response for protection against radiation-induced tratogenesis2005

    • Author(s)
      R.OKAZAKI
    • Journal Title

      Radiation Research 163・3

      Pages: 266-270

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Detection of radiation-induced mutations in the liver after partial hepatectomy using HITEC transgenic mice2005

    • Author(s)
      A.OOTSUYAMA
    • Journal Title

      International Congress Series 1276

      Pages: 260-261

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Effect of exposure to low-dose rate radiation on autoimmune diseases of immunologically suppressed MRL/MpTn-gld/gld mice2003

    • Author(s)
      A.OOTSUYAMA
    • Journal Title

      J. Radiation Research 44・3

      Pages: 243-247

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Effect of exposure to low-dose rate radiation on autoimmune diseases of immunologically suppressed MRL/MpTn-gld/gld mice.2003

    • Author(s)
      Ootsuyama, A., Okazaki, R., Norimura, T.
    • Journal Title

      J.Radiation Research 44(3)

      Pages: 243-247

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Publications] 欅田 尚樹: "放射線誘発突然変異の修復とp53遺伝子依存性アポトーシス"放射線生物研究. 38・4. 361-369 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] T.NORIMURA: "The effect of low dose rate on radiation-induced mutant frequency in mice : role of p53 gene (Abstract)"J.Radiat.Res.. 444. 376-376 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] T.NORIMURA: "Dose-rate effect in radiation mutagenesis : role of p53 gene"12th international congress of radiation research. Book of abstracts. 141-141 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 法村 俊之: "低線量・低線量率放射線による生物影響発現(大西武雄監修) 3-2-2.突然変異誘発の線量率効果"(株)アイプリコム. 62-64 (2003)

    • Related Report
      2003 Annual Research Report

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Published: 2003-04-01   Modified: 2016-04-21  

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