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Development of Surface Plasmon Resonance-type Quartz Crystal Microbalance and Detection of Interactions of Viomolecules

Research Project

Project/Area Number 15201025
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Nanomaterials/Nanobioscience
Research InstitutionTokyo Institute of Technology

Principal Investigator

OKAHATA Yoshio  Tokyo Institute of Technology, Frontier Collaborative Research Center, Professor, フロンティア創造共同研究センター, 教授 (80038017)

Co-Investigator(Kenkyū-buntansha) MORI Toshiaki  Tokyo Institute of Technology, Department of Biomolecular Engineering, Associate Professor, 大学院・生命理工学研究科, 助教授 (50262308)
FURUSAWA Hiroyuki  Tokyo Institute of Technology, Department of Biomolecular Engineering, Research Associate, 大学院・生命理工学研究科, 助手 (60345395)
KAWASAKI Takatoshi  Tokyo Institute of Technology, Department of Biomolecular Engineering, Research Associate, 大学院・生命理工学研究科, 助手 (60334504)
Project Period (FY) 2003 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥50,570,000 (Direct Cost: ¥38,900,000、Indirect Cost: ¥11,670,000)
Fiscal Year 2005: ¥14,690,000 (Direct Cost: ¥11,300,000、Indirect Cost: ¥3,390,000)
Fiscal Year 2004: ¥14,820,000 (Direct Cost: ¥11,400,000、Indirect Cost: ¥3,420,000)
Fiscal Year 2003: ¥21,060,000 (Direct Cost: ¥16,200,000、Indirect Cost: ¥4,860,000)
KeywordsQuartz crystal microbalance / Network analyzer / Energy dissipation factor / Conformation of Proteins / p53 Protein / タンパク質のコンフォメーション変化 / 表面プラズモン共鳴 / インピーダンスアナライザー / 分子認識
Research Abstract

By using a 27-MHz piezoelectric quartz oscillator connected with a vector network analyzer, we obtained resonance frequency decreases (-ΔF_<water>) and energy dissipation increases (ΔD_<water>) during binding of biotinylated BSA (bovine serum albumin), biotinylated ssDNA, biotinylated dsDNA, and biotinylated pullulan to a NeutrAvidin-immobilized 27-MHz QCM (quartz crystal microbalance) plate in aqueous solution, as well as in the wet air phase (97% humidity, -ΔF_<wet> and ΔD_<wet>) and in the dry air phase (-ΔF_<air> and ΔD_<air>). The -ΔF_<air> value indicates real mass changes due to the binding of these biomolecules. Values for polystyrene were also obtained as elastic and hydrophobic membranes. In terms of results, (-ΔF_<wet>)/(-ΔF_<air>) values indicated the binding water ratio per unit biomolecular mass were in the order of pullulan (2.1- 2.2) > DNAs = proteins (1.4- 1.6) > polystyrene (1.0). The (-ΔF_<water>)/(-ΔF_<air>) values indicated the hydrodynamic water (binding and vibrating water) ratio per unit biomolecular mass were in the order of dsDNA (6.5)> ssDNA = pullulan (3.5- 4.4) > proteins (2.4- 2.5) > polystyrene (1.0). The aforementioned values suggest that the energy dissipation of proteins was mainly caused by hydration and that proteins themselves are elastic molecules without energy dissipation in aqueous solutions. On the contrary, plots in cases of DNAs and pullulans were relatively deviant toward the large hydration and energy dissipation from the linearity observed with proteins, meaning that the large energy dissipation occurs because of viscoelastic properties of linear DNAs and pullulans in the water phase, in addition to energy dissipation due to the hydration of molecules.

Report

(4 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • 2003 Annual Research Report
  • Research Products

    (28 results)

All 2006 2005 2004 Other

All Journal Article (23 results) Publications (5 results)

  • [Journal Article] Evaluation of RNA Structures by using a Probe-immobilized 27 MHz Quartz Crystal Microbalance2006

    • Author(s)
      T.Ozeki, H.Furusawa, Y.Okahata
    • Journal Title

      Chem.Lett.

      Pages: 46-47

    • NAID

      10016907695

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Control of Hydrolysis and Condensation Activities of Thermolysin by Ultrasound Irradiation2006

    • Author(s)
      T.Kawasaki, Y.Hoshino, Y.Ishizu, Y.Mizushiro, Y.Okahata
    • Journal Title

      Chem.Lett. 34

      Pages: 1602-1603

    • NAID

      10016891573

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Kinetic Studies of Site-Direct Mutational Isomalto-dextranase Catalyzed Hydrolytic Reactions on a 27 MHz Quartz-crystal Microbalance2005

    • Author(s)
      T.Nihira, M.Mizuno, T.Tonozuka, Y.Sakano, T.Mori, Y.Okahata
    • Journal Title

      Biochemistry, 44

      Pages: 9456-9461

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] Kinetic Studies of DNA Cleavage Reactions catalyzed by an ATP-dependent Deoxyribonuclease on a 27-MHz Quartz-Crystal Microbalance2005

    • Author(s)
      H.Matsuno, H.Furusawa, Y.Okahata
    • Journal Title

      Biochemistry, 44

      Pages: 2262-2270

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] Control of Hydrolysis and Condensation Activities of Thermolysin by Ultrasound Irradiation2005

    • Author(s)
      T.Kawasaki, Y.Hoshino, Y.Ishizu, Y.Mizushiro, Y.Okahata
    • Journal Title

      Chem. Lett. 34

      Pages: 1602-1603

    • NAID

      10016891573

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Optimization of Nuclear Localization Signal for Nuclear Transport of DNA-encapsulating Particles2005

    • Author(s)
      A.Eguchi, H.Furusawa, A.Yamamoto, T.Akuta, M.Hasegawa, Y.Okahata, M.Nakanishi
    • Journal Title

      J. Controlled Release 104

      Pages: 507-519

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Adsorption behabior of poly(N-p-vinylbenzyl-4-o-beta-D-galactosyl-[1-4]-D-gluconamide) by quartz-crystal microbalance2005

    • Author(s)
      R.Takei, S.J.Seo, C.S.Cho, Y.Okahata, T.Akaike
    • Journal Title

      Colloids and Surfaces, B, m 42

      Pages: 137-140

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] DNA-Lipid Complexes in Organic Solution, and DNA-aligned Cast and LB Films2005

    • Author(s)
      Y.Okahata, T.Kawasaki
    • Journal Title

      Topics in Current Chemistry 260 "Immobilization of DNA on Chips I", ed by Christine Wittmann, Splinger-Verlag

      Pages: 57-75

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Gravimetric Analyses of Enzymatic Glucan Hydrolysis and Phosphorolysis on a 27 MHz Quartz-Crystal Microbalance2005

    • Author(s)
      T.Mori, Y.Okahata
    • Journal Title

      Trends in Glycoscience and Glycotechnology

      Pages: 71-83

    • NAID

      10015466864

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Kinetic Studies of Site-Direct Mutational Isomalto-dextranase Catalyzed Hydrolytic Reactions on a 27 MHz Quartz-crystal Microbalance2005

    • Author(s)
      T.Nihira, M.Mizuno, T.Tonozuka, Y.Sakano, T.Mori, Y.Okahata
    • Journal Title

      Biochemistry 44

      Pages: 9456-9461

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Kinetic Studies of DNA Cleavage Reactions catalyzed by an ATP-dependent Deoxyribonuclease on a 27-MHz Quartz-Crystal Microbalance2005

    • Author(s)
      H.Matsuno, H.Furusawa, Y.Okahata
    • Journal Title

      Biochemistry 44

      Pages: 2262-2270

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Control of Hydrolysis and Condensation Activites of Thermolysin by Ultrasound Irradiation2005

    • Author(s)
      T.Kawasaki, Y.Hoshino, Y.Ishizu, Y.Mizushiro, Y.Okahata
    • Journal Title

      Chem.Lett. 34

      Pages: 1602-1603

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Optimization of Nuclear Localization Signal for Nuclear Transport of DNA-encapsulating Particles2005

    • Author(s)
      Eguchi, H.Furusawa, A.Yamamoto, T.Akuta, M.Hasegawa, Y.Okahata, M.Nakanishi
    • Journal Title

      J.Controlled Release 104

      Pages: 507-519

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Adsorption behabior of poly(N-p-vinylbenzyl-4-o-beta-D-galactosyl-[1-4]-D-gluconamide) by quartz-crystal microbalance2005

    • Author(s)
      R.Takei, S.J.Seo, C.S.Cho, Y.Okahata, T.Akaike
    • Journal Title

      Colloids and Surfaces, B m42

      Pages: 137-140

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] DNA-Lipid Complexes in Organic Solution, and DNA-aligned Cast and LB Films2005

    • Author(s)
      Y.Okahata, T.Kawasaki
    • Journal Title

      Topics in Current Chemistry 260 "Immobilization of DNA on Chips I" (ed by Christine Wittmann) (Splinger-Verlag)

      Pages: 57-75

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Gravimetric Analyses of Enzymatic Glucan Hydrolysis and Phosphorolysis on a 27 MHz Quartz-Crystal Microbalance2005

    • Author(s)
      T.Mori, Y.Okahata
    • Journal Title

      Trends in Glycoscience and Glycotechnology 17

      Pages: 71-83

    • NAID

      10015466864

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Optimization of Nuclear Localization Signal for Nuclear Transport of DNA-encapsulating Particles2005

    • Author(s)
      A.Eguchi, H.Furusawa, A.Yamamoto, T.Akuta, M.Hasegawa, Y.Okahata, M.Nakanishi
    • Journal Title

      J.Controlled Release 104

      Pages: 507-519

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Adsorption behabior of poly(N-p-vinylbenzyl-4-o-beta-D-galactosyl-[1-4]-D-gluconamide) by quartz-crystal microbalance2005

    • Author(s)
      R.Takei, S.J.Seo, C.S.Cho, Y.Okahata, T.Akaike
    • Journal Title

      Colloids and Surfaces, B 42

      Pages: 137-140

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Direct Monitoring of Enzymatic Glucan Hydrolyses on a 27-MHz Quartz-Crystal Microbalance2004

    • Author(s)
      H.Nishino, T.Nihira, T.Mori, Y.Okahata
    • Journal Title

      J.Am.Chem.Soc. 126

      Pages: 2264-2265

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Kinetic Study of Phosphorylation-dependent Complex Formation between the Kinase-Inducible Domain (KID) of CREB and the KIX Domain of CBP on a Quartz C2004

    • Author(s)
      H.Matsuno, H.Furusawa, Y.Okahata
    • Journal Title

      Chem.Eur.J. 10

      Pages: 6172-6178

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Kinetic Studies of Amylopectin Cleavage Reactions Catalyzed by Phosphorylase b using a 27 MHz Quartz Crystal Microbalance2004

    • Author(s)
      H.Nishino, A.Murakawa, T.Mori, Y.Okahata
    • Journal Title

      J.Am.Chem.Soc. 126

      Pages: 14752-14757

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Interaction behavior and tensile strength of DNA-lipid films for the dental application2004

    • Author(s)
      T.Fukushima, T.Hayakawa, Y.Inoue, K.Miyazaki, Y.Okahata
    • Journal Title

      Biomaterials 25

      Pages: 5491-5497

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Enzymatic preparation of biotinylated naturally-occurring sialylglycan and its molecular recognition on a quartz-crystal microbalance2004

    • Author(s)
      T.Mori, Y.Sekine, K.Yamamoto, Y.Okahata
    • Journal Title

      Chem.Commun.

      Pages: 2692-2693

    • Related Report
      2004 Annual Research Report
  • [Publications] T.Ozeki, H.Furusawa, Y.Okahata: "In vitro selection of four way junction DNA"Nucleic Acids Res.Suppl.. No.3. 257-258 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] H.Furusawa, A.Murakawa, S.Fukusho, Y.Okahata: "In vitro Selection of N Peptide-Binding RNA on a Quartz-crystal Microbalance to Study a Sequence-Specific Interaction of Peptide and Loop RNA"ChemBioChem. 217-220 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Y.Inoue, T.Fukushima, T.Hayakawa, H.Takeuchi, H.Kaminishi, K.Miyazaki, Y.Okahata: "Antibacterial characteristics of newly developed amphiphilic lipids and DNA-lipid complexes against bacteria"J.Biomed.Mater.Res.. 65A. 203-208 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 川崎 剛美, 岡畑 恵雄: "サケ精子のDNAから作るバイオフィルム"未来材料. 3. 46-51 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 岡畑 恵雄: "DNAフィルム"化学装置. 4月号. 60-62 (2003)

    • Related Report
      2003 Annual Research Report

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Published: 2003-04-01   Modified: 2016-04-21  

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