| Project/Area Number |
15201045
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Living organism molecular science
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| Research Institution | Chiba University |
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Principal Investigator |
SAITO Kazuki Chiba University, Graduate School of Pharmaceutical Sciences, Professor, 大学院薬学研究院, 教授 (00146705)
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| Co-Investigator(Kenkyū-buntansha) |
YAMAZAKI Mami Chiba University, Graduate School of Pharmaceutical Sciences, Associate Professor, 大学院薬学研究院, 助教授 (70222370)
NOJI Masaaki Chiba University, Graduate School of Pharmaceutical Sciences, Research Associate, 大学院薬学研究院, 助手 (80271534)
TAKAYAMA Hiromitsu Chiba University, Graduate School of Pharmaceutical Sciences, Professor, 大学院薬学研究院, 教授 (90171561)
ARITA Masanori The University of Tokyo, Graduate School of Frontier Sciences, Associate Professor, 新領域創成科学研究科, 助教授 (10356389)
KANAYA Shigehiko Nara Institute of Science and Technology, Graduate School of Information Science, Professor, 情報科学研究科, 教授 (90224584)
山口 健太郎 千葉大学, 分析センター, 助教授 (50159208)
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| Project Period (FY) |
2003 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥41,860,000 (Direct Cost: ¥32,200,000、Indirect Cost: ¥9,660,000)
Fiscal Year 2006: ¥10,270,000 (Direct Cost: ¥7,900,000、Indirect Cost: ¥2,370,000)
Fiscal Year 2005: ¥10,270,000 (Direct Cost: ¥7,900,000、Indirect Cost: ¥2,370,000)
Fiscal Year 2004: ¥10,270,000 (Direct Cost: ¥7,900,000、Indirect Cost: ¥2,370,000)
Fiscal Year 2003: ¥11,050,000 (Direct Cost: ¥8,500,000、Indirect Cost: ¥2,550,000)
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| Keywords | metabolomics / transcriptomics / functional genomics / metabolism / Arabidopsis thaliana / 多変量解析 / 自己組織化マップ / 栄養ストレス / アントシアニン / アルカロイド / メタボローム / 機能ゲノミクス / グルコシノレート / DNAマイクロアレイ / フラボノイド |
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| Research Abstract |
Integrated analysis of omics can provide the clues for identification of gene function and precise information about gene-to-metabolite and/or metabolite-to-metabolite networks. We ran the functional genomics project combining metabolome and transcriptome in Arabidopsis thaliana. Nutritional stress of nitrogen and sulfur resulted in global change of metabolome that could be correlated with the modulation of gene expression, indicating the presence of gene-to-metabolite networks, in particular, in glucosinolate biosynthesis. The comprehensive gene expression and metabolite profiles of anthocyanin overproducing Arabidopsis lines revealed the function of novel genes that are responsible for modification and storage of anthocyanins. The function of those genes was identified by analysis of the T-DNA insertion lines and recombinant proteins. By expanding this strategy, we used a transcriptome database publicly available for functional identification of unknown genes and networks. Upon these co-expression analyses, the models of co-expressed genes in particular metabolic pathways were constructed, leading to speculation of functions of those genes followed by confirmation with reverse genetics strategy. Extensive metabolic profiling of the knockout mutants of genes of interest was performed by a metabolome-analytical platform employing mass spectrometry. Detailed metabolite analysis of the mutants revealed the changes in metabolo-types, indicating the gene functions. This strategy of integrated analysis of metabolome and transcriptome is applicable to decipher the function of genes not only for a model plant A. thaliana but also for unexplored plants expected rich with a variety of phychemicals.
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