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Development of Sensor System to Analyse and Diagnose the abnormality of Protein Conformation

Research Project

Project/Area Number 15206089
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Biofunction/Bioprocess
Research InstitutionOsaka University

Principal Investigator

KUBOI Ryoichi  Osaka University, Dept.Eng.Sci., Professor, 大学院・基礎工学研究科, 教授 (40029567)

Co-Investigator(Kenkyū-buntansha) TSUCHIDO Tetsuaki  Kansai University, Dept.Eng., Professor, 工学部, 教授 (50029295)
GOTO Yuji  Osaka University, Protein Institution, Professor, 蛋白質研究所, 教授 (40153770)
UMAKOSHI Hiroshi  Osaka University, Dept.Eng.Sci., Associate Professor, 大学院・基礎工学研究科, 助教授 (20311772)
YOSHIMOTO Makoto  Yamaguchi University, Dept.Eng., Eng., Associate Professor, 工学部, 助教授 (80322246)
SHIMANOUCHI Toshinori  Osaka University, Dept.Eng.Sci., Assistant Professor, 大学院・基礎工学研究科, 助手 (10335383)
Project Period (FY) 2003 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥43,680,000 (Direct Cost: ¥33,600,000、Indirect Cost: ¥10,080,000)
Fiscal Year 2005: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2004: ¥12,740,000 (Direct Cost: ¥9,800,000、Indirect Cost: ¥2,940,000)
Fiscal Year 2003: ¥27,300,000 (Direct Cost: ¥21,000,000、Indirect Cost: ¥6,300,000)
KeywordsMembrane Stress Biotechnology / Oxidative Stress / Amyloidogenic Protein / Stress Sensor / Conformational Disease / Hydrogen Bonding / Liposome / Fluctuation / タンパク質 / アミロイド / 局所的疎水性 / メンブレンストレスバイオテクノロジー / リポゾーム固定化技術 / 構造異常性疾患
Research Abstract

The goal of this research project is to establish the analytical method of dynamic interaction between the structurally abnormal protein and biomembrane under the stress condition, especially focusing on the local hydrophobicity (LH) or structural fluctuation. Amyloid beta peptide (Aβ) has been employed as a case study of the system to analyze and characterize the structural abnormality of various proteins. During past two years, we have developed the sensor system of the structural abnormality of protein and have established the database on the protein abnormality under the stress condition. In 2005, the method to judge the structural abnormality of proteins has been investigated based on the previous findings. Based on the database, the suitable conditions (stress condition and lipid compositions) to regenerate the conformational abnormality of protein under the stress condition on the liposome surface have been clarified, resulting that the microdomain-like structure of the membrane … More surface was found to play an important role on the conformational abnormality of proteins/peptides. It was furthermore found that the microdomain-like structure could suitably be controlled by the control of the stress conditions (heating, pH and ionic strength) and the modification of membrane components (alcohols, fatty acids, cholesterol). Especially in the case of the cholesterol-modified liposome, the Aβ-membrane interaction was found to be controlled on the membrane in the presence of metal ion, Cu. The Aβ-Cu complex furthermore induced the metalloenzyme-like function(oxidation of cholesterol and dopamine). The above potential functions of Aβ on the membrane was found to be closely related to the stability of hydrogen-bond of the main chain of the Aβ in the hydrophobic nano environment inside the liposome membrane. By using the membrane chip arraying various stressed-liposomes on the chip, it was found that we could assess the structural abnormality of the protein. The above findings on the structural abnormality of protein were summarized together with the physiological role of amyloid fibril formation of Aβ on the biomembrane. Less

Report

(4 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • 2003 Annual Research Report
  • Research Products

    (47 results)

All 2006 2005 2004 Other

All Journal Article (35 results) Book (5 results) Publications (7 results)

  • [Journal Article] Evaluation of Carboxylic Acid-Induced Conformational Transitions of β-lactoglobulin : Comparison of the Alcohol Effects on □-lactoglobulin2006

    • Author(s)
      Bong-Kuk Lee et al.
    • Journal Title

      Biochem.Eng.J. 28

      Pages: 79-86

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Evaluation of Temperature and Guanidine Hydrochloride-Induced Protein-Liposome Interactions by Using Immobilized Liposome Chromatography2006

    • Author(s)
      Nariko Yoshimoto, et al.
    • Journal Title

      Biochem.Eng.J. 29

      Pages: 174-181

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Analysis of Complex Permittivity of Liposome for Its Biochemical Dynamics up to 30 GHz Range2006

    • Author(s)
      Minoru Noda, et al.
    • Journal Title

      IEEE (In press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] Evaluation of Carboxylic Acid-Induced Conformational Transitions of β-lactoglobulin : Comparison of the Alcohol Effects on □-lactoglobulin2006

    • Author(s)
      Bong Kuk Lee et al.
    • Journal Title

      Biochem.Eng.J. 28

      Pages: 79-86

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Evaluation of Temperature and Guanidine Hydrochloride-Induced Protein-Liposome Interactions by Using Immobilized Liposome Chromatography2006

    • Author(s)
      Noriko Yoshimoto, et al.
    • Journal Title

      Biochem.Eng.J. 29

      Pages: 174-181

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Analysis of Complex Permittivity of Liposome for Its Biochemical Dynamics up to 30 GHz Range2006

    • Author(s)
      Minoru Noda, et al.
    • Journal Title

      IEEE (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Evaluation of Carboxylic Acid-Induced Conformational Transitions of β-lactoglobulin : Comparison of the Alcohol Effects on β-lactoglobulin2006

    • Author(s)
      Bong-Kuk Lee et al.
    • Journal Title

      Biochem.Eng.J. 28

      Pages: 79-86

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Evaluation of Temperature and Guanidine Hydrochloride-Induced Protein-Liposome Interactions by Using Immobilized Liposome Chromatography2006

    • Author(s)
      Noriko Yoshimoto, et al.
    • Journal Title

      Biochem.Eng.J. (In press)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Liposome-Assisted Activity of Superoxide Dismutase under Oxidative Stress2005

    • Author(s)
      Hideto Nagami, et al.
    • Journal Title

      J.Biosci.Bioeng. 99(4)

      Pages: 423-428

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] Oxidation of Cholesterol Catalyzed by A□-Cu Complex on Lipid Membrane2005

    • Author(s)
      Nariko Yoshimoto, et al.
    • Journal Title

      J.Biosci.Bioeng. 100(5)

      Pages: 455-459

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Novel Immobilization Liposomal Gulcose Oxidase System, Using the Channel Protein OmpF and Catalase2005

    • Author(s)
      Makoto Yoshimoto, et al.
    • Journal Title

      Biosci.Bioeng. 90(2)

      Pages: 231-238

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Heat-Enhanced Production of Chitosanase from Streptonzyces griseus in the Presence of Linosome2005

    • Author(s)
      Kien Xuan Ngo, et al.
    • Journal Title

      J.Biosci.Bioeng. 100(5)

      Pages: 495-501

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] 「研究成果報告書概要(和文)」より2005

    • Author(s)
      鳥内 寿徳 他
    • Journal Title

      分離技術 35(6)

      Pages: 391-396

    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Detection of Conformational Change of Protein Based on Protein-Liposome Interaction2005

    • Author(s)
      Ryoich Kuboi, et al.
    • Journal Title

      Proc.ICSST05-KJ

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] Metal affinity-immobilized liposome chromatography for detection of conformational change of peptide2005

    • Author(s)
      Ryoich Kuboi, et al.
    • Journal Title

      Proc.ICSST05-KJ

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] Heat enhanced recovery of chitosanase from Streptomyces griceus using aqueous two-phase system2005

    • Author(s)
      Kien Xuan Ngo, et al.
    • Journal Title

      Proc.ICSST05-KJ

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] Oxidation of Cholesterol Catalyzed by Aβ-Cu Complex on Lipid Membrane2005

    • Author(s)
      Noriko Yoshimoto, et al.
    • Journal Title

      J.Biosci.Bioeng. 100(5)

      Pages: 455-459

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Heat-Enhanced Production of Chitosanase from Streptomyces griseus in the Presence of Liposome2005

    • Author(s)
      Kien Xuan Ngo, et al.
    • Journal Title

      J.Biosci.Bioeng. 100(5)

      Pages: 495-501

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Detection of Conformational Change of Protein Based on Protein-Liposome Interaction2005

    • Author(s)
      Ryoich Kuboi, et al.
    • Journal Title

      Proc.ICSST05-KJ AD114(9)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Metal affinity-immobilized liposome chromatography for detection of conformational change of peptide2005

    • Author(s)
      Ryoich Kuboi, et al.
    • Journal Title

      Proc.ICSST05-KJ AD01(8)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Heat enhanced recovery of chitosanase from Streptomyces griseus using aqueous two-phase system2005

    • Author(s)
      Kien Xuan Ngo, et al.
    • Journal Title

      Proc.ICSST05-KJ EX09(8)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Oxidation of Cholesterol Catalyzed by Aβ-Cu Complex on Lipid Membrane2005

    • Author(s)
      Nariko Yoshimoto, et al.
    • Journal Title

      J.Biosci.Bioeng. 100(5)

      Pages: 455-459

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Novel Immobilization Liposomal Gulcose Oxidase System, Using the Channel Protein -OmpF and Catalase2005

    • Author(s)
      Makoto Yoshimoto, et al.
    • Journal Title

      Biotech.Bioeng. 90(2)

      Pages: 231-238

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Heat-Enhanced Production of Chitosanase from Streptomyces griseus.in the Presence of Liposome2005

    • Author(s)
      Kien Xuan Ngo, et al.
    • Journal Title

      J.Biosci.Bioeng. 100(5)

      Pages: 495-501

    • Related Report
      2005 Annual Research Report
  • [Journal Article] タンパク質の構造異常疾患におけるアミロイド線維の制御〜メンブレン・ストレスバイオテクノロジーの視点から〜2005

    • Author(s)
      島内 寿徳 他
    • Journal Title

      分離技術 35(6)

      Pages: 391-396

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Comparison of the Extraction Behavior of Metal Ions by Metal Affinity Ligands in An Organic-Aqueous Two-Phase System and in A Liposome System2004

    • Author(s)
      H.Nagami, H.Umakoshi, T.Shimanouchi, R.Kuboi, Y.Baba
    • Journal Title

      Solv.Extr.Res.Dev.Japan 11

      Pages: 159-165

    • NAID

      40006306590

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Enhancement of apparent substrate selectivity of proteinase K encapsulated in liposomes through a cholate-induced alteration of the bilaver permeability2004

    • Author(s)
      M.Yoshimoto, S.Wang, K.Fukunaga, M.Treyer, P.Walde, R.Kuboi, K.Nakao
    • Journal Title

      Biotechnology and Bioengineering 85(2)

      Pages: 222-233

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Evaluation of Carboxylic Acid-Induced Formation of Reverse Micelle Clusters : Comparison of the Effects of Alcohols on Reverse Micelles2004

    • Author(s)
      B.-K.Lee, D.-P.Hong, S-S.Lee, R.Kuboi
    • Journal Title

      Biochem.Eng.J. 21

      Pages: 11-18

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Relationship between Structure of Metal Chelating Ligands and Their Extraction Ability for Ag(I). Hg(II). Pd(II). and Au(III)2004

    • Author(s)
      H.Nagami, Y.Baba, R.Kuboi
    • Journal Title

      Solv.Extr.Res.Dev.Japan 11

      Pages: 167-182

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Variable SOD-Like Activity of Liposome Modified with Mn(II)-Porphyrin Derivative Complex2004

    • Author(s)
      H.Nagami, H.i Umakoshi, T.Shimanouchi, R.Kuboi
    • Journal Title

      Biochem.Eng.J. 21

      Pages: 221-227

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Mechanism for High Stability of Liposomal Glucose Oxidase to Inhibitor Hydrogen Peroxide Produced in Prolonged Glucose Oxidation2004

    • Author(s)
      M.Yoshimoto, Y.Miyazaki, M.Sato, K.Fukunaga, R.Kuboi, K.Nakao
    • Journal Title

      Bioconj.Chem. 15(5)

      Pages: 1055-1061

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Analysis of protein back-extraction processes in alcohol-and carboxylic acid-mediated AOT reverse micellar systems based on structural changes of proteins and reverse micelles2004

    • Author(s)
      B.-K.Lee, D.-P.Hong, S.-S.Lee, R.Kuboi
    • Journal Title

      Biochem.Eng.J. 22

      Pages: 71-79

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Detection of Protein Conformation under Stress Condition Using Liposome as Sensor Materials2004

    • Author(s)
      R.Kuboi, T.Shimanouchi, M.Yoshimoto, H.Umakoshi
    • Journal Title

      Sensors and Materials 16(5)

      Pages: 241-254

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Development of Ppy Films Doped with Thiol-SAM-Cu Particles for NH_3 Gas Sensing2004

    • Author(s)
      T.Shimanouchi, S.Morita, H.-S.Jung, Y.Sakurai, Y.Suzuki, R.Kuboi
    • Journal Title

      Sensors and Materials 16(5)

      Pages: 255-265

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Novel Immobilization Liposomal Glucose Oxidase System, Using the Channel Protein OmpF and Catalase

    • Author(s)
      Makoto Yoshimoto, et al.
    • Journal Title

      Biotech.Bioeng. 90(2)

      Pages: 231-238

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Book] リポソーム応用の新展開〜人工細胞の開発に向けて(分担執筆)固定化リポソームセンサとタンパク質の構造異常性診断2005

    • Author(s)
      久保井 亮一 他
    • Publisher
      NTS出版
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Book] 進化する有機半導体(分担:導電性高分子/人工細胞膜を用いたストレスセンサの開発)2005

    • Author(s)
      久保井 亮一 他
    • Publisher
      NTS出版
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Book] リポソーム応用の新展開〜人工細胞の開発に向けて(分担執筆)固定化リポソームセンサとタンパク質の構造異常性診断2005

    • Author(s)
      久保井 亮一
    • Publisher
      エヌ・ティー・エス出版(印刷中)(未定)
    • Related Report
      2004 Annual Research Report
  • [Book] 生物工学会誌「特集 メンブレン・ストレスバイオテクノロジー」2005

    • Author(s)
      久保井 亮一
    • Total Pages
      21
    • Publisher
      日本生物工学会
    • Related Report
      2004 Annual Research Report
  • [Book] ケミカルエンジニヤリング「メンブレン・ストレスバイオテクノロジー〜薬物送達システムの設計と開発への応用〜」2004

    • Author(s)
      久保井 亮一
    • Total Pages
      6
    • Publisher
      化学工業社
    • Related Report
      2004 Annual Research Report
  • [Publications] H-S.Jung, et al.: "Novel Fabrication of Conductive Polymers Contained Micro-Array Gas Sensitive Films Using A Micromanipulation Method"Electroanalysis. 15(18). 1453-1459 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] M.Yoshimoto et al.: "Preparation and Characterization of Reactive and Stable Glucose Oxidase-Containing Liposomes Modulated with Detergent"Biotechnology and Bioengineering. 81(6). 695-704 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] S.Morita et al.: "Detection of a Heat Stress-Mediated Interaction between Protein and Phospholipid Membrane Using Dielectric Measurement"Journal of Bioscience and Bioengineering. 93(5). 252-256 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] N.Yoshimoto et al.: "Artificial Chaperone-Assisted Refolding of Bovine Carbonic Anhydrase Using Molecular Assemblies of Stimuli-Responsive Polymers"Biomacromolecules. 4(6). 1530-1538 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] M.Menayame F.et al.: "Evaluation of Interaction between Liposome Membranes Induced by Stimuli Responsive Polymer and Protein"Journal of Bioscience and Bioengineering. 93(5). 498-501 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] M.Yoshimoto et al.: "Enhancement of Apparent Substrate Selectivity of Proteinase K Encapsulated in Liposome through a Cholate-Induced Alteration of the Bilaver Permeability"Biotechnology and Bioengineering. 85(2). 222-233 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] 久保井 亮一: "固定化リポソームクロマトグラフィーによる生理活性物質の分離・分析"日本分析化学会(ぶんせき, 2003年11号). 8(636-644) (2003)

    • Related Report
      2003 Annual Research Report

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Published: 2003-04-01   Modified: 2016-04-21  

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