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Mechanism of formation of the contractile ring during cytokinesis

Research Project

Project/Area Number 15207013
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Cell biology
Research InstitutionThe University of Tokyo

Principal Investigator

MABUCHI Issei  University of Tokyo, School of Arts and Sciences, Professor, 大学院・総合文化研究科, 教授 (40012520)

Project Period (FY) 2003 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥49,400,000 (Direct Cost: ¥38,000,000、Indirect Cost: ¥11,400,000)
Fiscal Year 2005: ¥8,970,000 (Direct Cost: ¥6,900,000、Indirect Cost: ¥2,070,000)
Fiscal Year 2004: ¥20,930,000 (Direct Cost: ¥16,100,000、Indirect Cost: ¥4,830,000)
Fiscal Year 2003: ¥19,500,000 (Direct Cost: ¥15,000,000、Indirect Cost: ¥4,500,000)
Keywordsgene / enzyme / cell / tissue / signal transduction / protein / cytokinesis / actin / myosin / 酵母 / 分裂シグナル / 低分子量Gタンパク質 / Rho / アクチン調節タンパク質
Research Abstract

1)rho4 and rho5 genes of fission yeast was cloned. Rho4 regulates localization of F-actin patches and organization of microtubules, and thereby formation of cell wall and the septum. Localization of Rho4 is regulated by RhoGDI while activity of Rho4 is regulated by the RhoGAP Rga9
2)Sea urchin Cdc42 showed 87% homology to human Cdc42. It binds IQGAP and thus enhancesw actin polymerization. IQGAP localizes to the cleavage furrow during cytokinesis.
3)It was established that the C-terminal 134 residues are necessary and sufficient for localization of Myo2 to the division site. This region contains Ser1444 which is phosphorylated during interphase but dephosphorylated during M-phase. This dephosphorylation enables Myo2 to target Mid1 which is transmitted from the nucleus to the equatorial cortex during G2.
4)The Rho1GEFs Rgf1 and Rgf2 are localized to the growing ends of fission yeast cell during interphase, while to the septum region during mitosis and are involved in synthesis of cell wall … More and the septum. Rgf3 accumulates at the division site forming a ring during mitosis, and the ring contracts as cytokinesis progresses. Rgf3 is considered to control formation of both the contractile ring and the septum through activation of Rho1.
5)We determined directionality of F-actins in the F-actin cables in fission yeast cells by decorating the F-actins using myosin S1. The barbed end of the F-actins in the interphase cables face the growing end of the cell during interphase. During mitosis, it reversed. We proposed a hypothesis that myosin V transports materials necessary for polarized growth of the cell to the cell ends during interphase, while transports those necessary for division to the division site during mitosis.
6)Fission yeast actin-depolymerizing factor Adf1 is localized to the F-actin patches during interphase and to the contractile ring during mitosis and cytokinesis. Adf1 is involved in formation of the contractile ring through its F-actin-severing activity. Its binding to F-actin in the cell is competitive with the tropomyosin Cdc8. It was also found that Adf1 cooperates with myosin-II in formation of the contractile ring. We propose that Adf1 is necessary for formation and stability of the contractile ring. Less

Report

(4 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • 2003 Annual Research Report
  • Research Products

    (26 results)

All 2006 2005 2004 2003 Other

All Journal Article (19 results) Book (2 results) Publications (5 results)

  • [Journal Article] Actin-depolymerizing protein Adf1 is required for formation and maintenance of the contractile ring during cytokinesis in fission yeast.2006

    • Author(s)
      Nakano, K
    • Journal Title

      Molecular Biology of the Cell 17

      Pages: 1933-1945

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Actin-depolymerizing protein Adf1 is required for formation and maintenance of the contractile ring during cytokinesis in fission yeast.2006

    • Author(s)
      Nakano, K., Mabuchi, I.
    • Journal Title

      Molecular Biology of the Cell 17

      Pages: 1933-1945

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Actin-depolymerizing protein Adf1 is required for formation and maintenance of the contractile ring during cytokinesis in fission yeast.2006

    • Author(s)
      Nakano, K.
    • Journal Title

      Molecular Biology of the Cell 17(In press)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Rho1GEFs Rgf1 and Rgf2 are involved in formation of cell wall and septum, while Rgf3 is involved in cytokinesis in fission yeast.2005

    • Author(s)
      Mutoh, T.
    • Journal Title

      Genes to Cell 10

      Pages: 1189-1202

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Small GTPasc Rho4 ia a functional homolog of Rho1, which controls cell shape and septation in fission yeast.2005

    • Author(s)
      Nakano, K.
    • Journal Title

      FEBS Letters 579

      Pages: 5181-5186

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Directionality of F-actin cables changes during the fission yeast cell cycle.2005

    • Author(s)
      Kamasaki, T.
    • Journal Title

      Nature Cell Biology 7

      Pages: 916-917

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] Rho1GEFs Rgf1 and Rgf2 are involved in formation of cell wall and septum, while Rgf3 is involved in cytokinesis in fission yeast.2005

    • Author(s)
      Mutoh, T., Nakano, K., Mabuchi, I.
    • Journal Title

      Genes to Cells 10

      Pages: 1189-1202

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Small GTPase Rho5 is a functional homolog of Rho1, which controls cell shape and septation in fission yeast.2005

    • Author(s)
      Nakano, K., Arai, R., Mabuchi, I.
    • Journal Title

      FEBS Letters 579

      Pages: 5181-5186

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Directionality of F-actin cables changes during the fission yeast cell cycle.2005

    • Author(s)
      Kamasaki, T., Arai, R., Osunii, M., Mabuchi, I.
    • Journal Title

      Nature Cell Biology 7

      Pages: 916-917

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Rho1GEFs Rgf1 and Rgf2 are involved in formation of cell wall and septum, while Rgf3 is involved in cytokinesis in fission yeast.2005

    • Author(s)
      Mutoh, T.
    • Journal Title

      Genes to Cells 10

      Pages: 1189-1202

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Plant villin, lily P-135-ABP, possesses G-actin binding activity and accelerates the polymerization and depolymerization of actin in a Ca2+-sensitive manner.2005

    • Author(s)
      Yokota, E.
    • Journal Title

      Plant and Cell Physiology 46

      Pages: 1690-1703

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Small GTPase Rho5 is a functional homolog of Rho1, which controls cell shape and septation in fission yeast.2005

    • Author(s)
      Nakano, K.
    • Journal Title

      FEBS Letters 579

      Pages: 5181-5186

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Myosin-II reorganization during mitosis is controlled temporally by its dephosphorylation and spatially by Mid1 in fission yeast.2004

    • Author(s)
      Motegi, F.
    • Journal Title

      J. Cell Biology 165

      Pages: 685-695

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Myosin-II reorganization during mitosis is controlled temporally by its dephosphorylation and spatially by Mid1 in fission yeast.2004

    • Author(s)
      Motcgi, F., Mishra, M., Balasubramanian, M.K., Mabuchi, I.
    • Journal Title

      J.Cell Biology 165

      Pages: 685-695

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] A novel actin-bunding kinesin-related protein from Dictyostelium discoideum.2004

    • Author(s)
      Iwai, S.
    • Journal Title

      J.Biol.Chem 279

      Pages: 4696-4704

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Myosin-II reorganization during mitosis is controlled temporally by its dephosporylation and spatially by mid1 in fission yeast.2004

    • Author(s)
      Motegi, F.
    • Journal Title

      J.Cell Biol. 165

      Pages: 685-695

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Biomedical research publication system.2004

    • Author(s)
      Wang, Y.-L
    • Journal Title

      Science 303

      Pages: 1974-1975

    • Related Report
      2004 Annual Research Report
  • [Journal Article] The small GTPase Rho4 is involved in controlling cell morphology and septation in fssion yeast2003

    • Author(s)
      Nakano, K.
    • Journal Title

      Genes to Cells 8

      Pages: 357-370

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] The small GTPase Rho4 is involved in controlling cell morphology and septation in fssion yeast2003

    • Author(s)
      Nakano, K., Mutoh, T., Arai, R., Mabuchi, I.
    • Journal Title

      Genes to Cells 8

      Pages: 357-370

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Book] 細胞生物学事典2005

    • Author(s)
      馬渕一誠(執筆分担)
    • Total Pages
      464
    • Publisher
      朝倉書店
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Book] 細胞生物学事典2005

    • Author(s)
      馬渕一誠
    • Total Pages
      464
    • Publisher
      朝倉書店
    • Related Report
      2004 Annual Research Report
  • [Publications] Sawako Yamashiro: "Localization of two IQGAPs in cultured cells and early embryos of Xenopus laevis"Cell Motility and the Cytoskeleton. 55. 36-50 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kentaro Nakano: "The small GTPase Rho4 is involved in controlling cell morphology and septation in fission yeast."Genes to Cells. 8. 357-370 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Yukako Nishimura: "An IQGAP-like protein is involved in actin assembly together with Cdc42 in the sea urchin egg."Cell Motility and the Cytoskeleton. 56. 207-218 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 馬渕 一誠: "細胞が分裂する時、収縮環はどのようにして形成されるか"学術月報. 56. 1243-1252 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 山城佐和子: "タンパク質がわかる"羊土社. 132 (2003)

    • Related Report
      2003 Annual Research Report

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Published: 2003-04-01   Modified: 2016-04-21  

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