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Functional characterization of peroxisome proliferator-activated receptor (PPAR) in chickens -Regulation of PPAR gene expression in embryo and early growth stages and utilization for high quality animal products-

Research Project

Project/Area Number 15208026
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied animal science
Research InstitutionTohoku University

Principal Investigator

AKIBA Yukio  Tohoku University, Graduate School of Agricultural Science, Professor, 大学院・農学研究科, 教授 (30005631)

Co-Investigator(Kenkyū-buntansha) TOYOMIZU Masaaki  Tohoku University, Graduate School of Agricultural Science, Associate Professor, 大学院・農学研究科, 助教授 (80180199)
SATO Kan  Tohoku University, Graduate School of Agricultural Science, Assistant Professor, 大学院・農学研究科, 助手 (20250730)
ABE Hiroyuki  National Agriculture and Food Research Organization, NILGS, Team Leader, 農業・生物系特定産業技術研究機構・畜産草地研究所, 室長 (30391379)
Project Period (FY) 2003 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥46,800,000 (Direct Cost: ¥36,000,000、Indirect Cost: ¥10,800,000)
Fiscal Year 2005: ¥10,140,000 (Direct Cost: ¥7,800,000、Indirect Cost: ¥2,340,000)
Fiscal Year 2004: ¥14,690,000 (Direct Cost: ¥11,300,000、Indirect Cost: ¥3,390,000)
Fiscal Year 2003: ¥21,970,000 (Direct Cost: ¥16,900,000、Indirect Cost: ¥5,070,000)
KeywordsPPAR / glucose metabolism / adipocyte / skeletal muscle / high-quality meat production / chickens / Transfection / エネルギー代謝 / 骨格筋細胞
Research Abstract

Peroxisome proliferation-activated receptors (PPAR) are a transcription factor that modulates energy, lipid and glucose metabolism in mammals. The aim of the present study was to investigate whether chicken PPARs is involved in the regulation of energy, lipid and glucose metabolism and whether modification of th gene expression in embryo and post-hatched chicks is utilized to build the high-quality animal production.
(1) Regulation of uncoupling protein and the co-factor by PPARs in chicken skeletal muscles
In chickens, avUCP and avANT play important roles of energy metabolism in mitochondria of chickens. The expression of avUCP was increased with the increase of PGC-1α gene expression under the cold acclimation for 7days. In the condition of starvation, PPARs and avANT gene expressions were lineally increased until 48h of starvations. These results suggested that the factors involved in the uncoupling of mitochondria membrane (avUCP and avANT) are regulated by PPARs and/or these co-fact … More ors.
(2) Physiological function of PPARs in chicken adipocyte differentiation
The present studies showed that PPARγ is a key regulator of adipocyte differentiation during fatty acid-induced adipogensis in chickens. PPARβ/δ stimulated the chicken adipogenesis induced by activation of PPARγ. Thus, PPARs play a crucial role in the regulation of chicken adipogenesis.
(3) Improvement in insulin-stimulated glucose transport in skeletal muscles of chickens administrated with PPARs agonist
Troglitazone, a PPARγ synthetic ligand, improved tolerance to glucose and insulin administration through modulation of skeletal muscle glucose transport, with these effects being accompanied by changes in plasma adiponectin and NEFA levels. These results suggested that PPARγ is involved in the carbohydrate metabolism species-specific to chickens.
(4) Development of high quality meat production system by activation of PPAR in post-hatched chicks
The single intraperitoneal administration of Troglitazone, a PPARγ synthetic ligand, to newly hatched chicks resulted the reduction of fat deposition and the increase of feed utilization in chickens at 7 week of age. It is, therefore, likely that the regulation and modification of PPARs expressions and activities might be useful technique for high-quality chicken meat production. Less

Report

(4 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • 2003 Annual Research Report
  • Research Products

    (30 results)

All 2005 2004 2003 Other

All Journal Article (27 results) Publications (3 results)

  • [Journal Article] Possible role for avPGC-1alpha in the control of expression of fiber type, along with avUCP and avANT mRNAs in the skeletal muscles of cold-exposed chickens.2005

    • Author(s)
      Ueda M, Watanabe K, Sato K, Akiba Y, Toyomizu M.
    • Journal Title

      FEBS Lett. 579

      Pages: 11-17

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Modulation of regulatory factors involved in cholesterol metabolism in response to feeding of pravastatin- or cholesterol-supplemented diet in chickens.2005

    • Author(s)
      Matsuyama H., Sato K., Akiba Y.et al.
    • Journal Title

      Biochim Biophys Acta 1734

      Pages: 136-142

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] 家禽における分子栄養学のフロンティア2005

    • Author(s)
      佐藤 幹
    • Journal Title

      日本家禽学会誌 42

    • NAID

      10014491325

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] Octanoate reduces very low-density lipoprotein secretion by decreasing the synthesis of apolipoprotein B in primary cultures of chicken hepatocytes.2005

    • Author(s)
      Tachibana S., Sato K., Akiba Y.et al.
    • Journal Title

      Biochim Biophys Acta 1737

      Pages: 36-43

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Changes in mRNA expression of regulatory factors involved in adipocyte differentiation during fatty acid induced adipogenesis in chicken.2005

    • Author(s)
      Matusbara Y., Sato K., Akiba Y.et al.
    • Journal Title

      Comp Biochem Physiol A 141

      Pages: 108-115

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Impairment of very low density lipoprotein secretion by medium chain fatty acids in chicken primary hepatocytes is affected by the chain length.2005

    • Author(s)
      Sato K., Akiba Y.et al.
    • Journal Title

      J. Nutrion 135

      Pages: 1636-1641

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Changes in muscle mRNAs for hexokinase, phosphofructokinase-1 and glycogen synthase in acute and persistent hypoglycemia induced by tolbutamide in chickens.2005

    • Author(s)
      Seki Y., Sato K., Akiba Y.
    • Journal Title

      Comp Biochem Physiol B 142

      Pages: 201-208

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] Possible role for avPGC-1α in the control of expression of fiber type, along with avUCP and avANT mRNAs in the skeletal muscles of cold-exposed chickens.2005

    • Author(s)
      Ueda M., Watanabe K., Sato K, Akiba Y, Toyomizu M.
    • Journal Title

      FEBS Letters 579

      Pages: 11-17

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Modulation of regulatory factors involved in cholesterol metabolism in response to feeding of pravastatin- or cholesterol-supplemented diet in chickens.2005

    • Author(s)
      Matsuyama H., Sato K., Nakamura Y, Suzuki K., Akiba Y.
    • Journal Title

      Biochim Biophys Acta 1734

      Pages: 136-142

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Frontier of molecular nutrition in poultry science2005

    • Author(s)
      Sato K.
    • Journal Title

      J.Poult.Sci. 42

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Octanoate reduces very low-density lipoprotein secretion by decreasing the synthesis of apolipoprotein B in primary cultures of chicken hepatocytes.2005

    • Author(s)
      Tachibana S., Sato K., Cho Y., Chiba T., Schneider WJ., Akiba Y.
    • Journal Title

      Biochim Biophys Acta 1737

      Pages: 36-43

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Changes in mRNA expression of regulatory factors involved in adipocyte differentiation during fatty acid induced adipogenesis in chicken.2005

    • Author(s)
      Matsubara Y., Sato K, Ishii H., Akiba Y.
    • Journal Title

      Comp.Biochem.Physiol.A 141

      Pages: 108-115

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Impairment of very low density lipoprotein secretion by medium chain fatty acids in chicken primary hepatocytes is affected by the chain length.2005

    • Author(s)
      Sato K., Cho Y, Tachibana S., Chiba T., Schneider W.J., Akiba Y.
    • Journal Title

      J.Nutr. 135

      Pages: 1636-1641

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Changes in muscle mRNAs for hexokinase, phosphofructokinase-1 and glycogen synthase in acute and persistent hypoglycemia induced by tolbutamide in chickens.2005

    • Author(s)
      Seki Y, Sato K, Akiba Y
    • Journal Title

      Comp Biochem Physiol B, Biochem Mol Biol. 142

      Pages: 201-208

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Octanoate reduces very low-density lipoprotein secretion by decreasing the synthesis of apolipoproten B in primary cultures of chicken hepatocytes.2005

    • Author(s)
      Tachiabna S., Sato K., Akiba Y.et al.
    • Journal Title

      Biochim Biophys Acta 1737

      Pages: 36-43

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Changes in mRNA expression of regulatory factors involved in adipocyte differentiation during fatty acid induced adipogenesis in chicken.2005

    • Author(s)
      Matsubara Y., Sato K., Akiba Y.et al.
    • Journal Title

      Comp Biochem Physiol A 141

      Pages: 108-115

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Impairment of very low density lipoprotein secretion by medium chain fatty acids in chicken primary hepatocytes is affected by the chain length.2005

    • Author(s)
      Sato K., Akiba Y.et al.
    • Journal Title

      J.Nutr. 135

      Pages: 1636-1641

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Possible role for avPGC-1alpha in the control of expression of fiber type, along with avUCP and avANT mRNAs in the skeletal muscles of cold-exposed chickens.2005

    • Author(s)
      Ueda M, Watanabe K, Sato K, Akiba Y, Toyomizu M
    • Journal Title

      FEBS Lett. 579

      Pages: 11-17

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Expression of the chicken peroxisome proliferator-activated receptor-gamma gene is influenced by aging, nutrition, and agonist administration.2004

    • Author(s)
      Sato K, Fukao K, Seki Y, Akiba Y.
    • Journal Title

      Poult Sci. 83

      Pages: 1342-1347

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Expression of the chicken peroxisome proliferator-activated receptor gamma gene is influenced by aging, nutrition and agonist administration.2004

    • Author(s)
      Sato K., Fukao K., Seki Y., Akiba Y.
    • Journal Title

      Poult Sci. 83

      Pages: 1342-1347

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Expression of the chicken peroxisome proliferator-activated receptor-gamma gene is influenced by aging, nutrition, and agonist administration.2004

    • Author(s)
      Sato K, Fukao K, Seki Y, Akiba Y
    • Journal Title

      Poult Sci. 83

      Pages: 1342-1347

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Insulin-glucose interactions characterized in newly-hatched broiler chicks.2003

    • Author(s)
      Tokushima, Y., Sulistiyanto, B., Takahashi, K., Akiba, Y.
    • Journal Title

      Br. Poultry Sci. 44

      Pages: 746-751

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] 鶏脂質代謝の特性とその調節2003

    • Author(s)
      佐藤幹, 秋葉征夫
    • Journal Title

      畜産技術 577

      Pages: 2-6

    • NAID

      40005835991

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Broiler chickens (Ross strain) lack insulin-responsive glucose transporter GLUT4 and have GLUT8 cDNA2003

    • Author(s)
      Seki, Y., Sato, K., Kono, T., Abe, H., Akiba, Y.
    • Journal Title

      Gen Comp Endocrinol. 133

      Pages: 80-87

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Insulin-glucose interactions characterized in newly-hatched broiler chicks.2003

    • Author(s)
      Tokushima, Y., Sulistiyanto, B., Takahashi, K., Akiba, Y.
    • Journal Title

      Br.Poult.Sci. 44

      Pages: 746-751

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Characterization and Regulation of lipid metabolism in chickens.2003

    • Author(s)
      Sato, K., Akiba, Y.
    • Journal Title

      Tikusan-Gizyutu 577

      Pages: 2-6

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Broiler chickens (Ross strain) lack insulin-responsive glucose transporter GLUT4 and have GLUT8 cDNA.2003

    • Author(s)
      Seki Y, Sato K., Kono T, Abe H., Akiba Y.
    • Journal Title

      Gen.Comp.Endocrinol. 133

      Pages: 80-87

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Publications] Tokushima, Y., Sulisutiyanto, B., Takahashi, K., Akiba, Y.: "Insulin-glucose interactions characterized in newly-hatched broiler chicks"Br.Poultry Sci.. 44. 746-751 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 佐藤幹, 秋葉征夫: "鶏脂質代謝の特性とその調節"畜産技術. 577. 2-6 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Seki, Y., Sato, K., Kono, T., Abe, H., Akiba, Y.: "Broiler chickens (Ross strain) lack insulin-responsive glucose transporter GLUT4 and have GLUT8 cDNA"Gen Comp Endocrinol.. 133. 80-87 (2003)

    • Related Report
      2003 Annual Research Report

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Published: 2003-04-01   Modified: 2016-04-21  

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