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Developments of Anti-tick Vaccine and Bio-acaricides Using Recombinant Virus of Tick Bioactive Molecules

Research Project

Project/Area Number 15208028
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied veterinary science
Research InstitutionKagoshima University (2006)
Obihiro University of Agriculture and Veterinary Medicine (2003-2005)

Principal Investigator

FUJISAKE Kozo  Kagoshima University, Department of Frontier Veterinary Medicine, Professor, 農学部, 教授 (00292095)

Co-Investigator(Kenkyū-buntansha) SUGIMOTO Chihiro  Hokkaido University, Research Center for Zoonosis Control, Professor, 人獣共通感染症リサーチセンター, 教授 (90231373)
TSUJI Naotoshi  National Agricultural Research Organization., National Institute of Animal Health, Senior researcher, 動物衛生研究所, 主任研究員 (70355171)
YOKOYAMA Naoaki  Obihiro University of Agriculture and Veterinary Medicine, National Research Center for Protozoan Diseases, Associate Professor, 原虫病研究センター, 助教授 (80301802)
Project Period (FY) 2003 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥46,150,000 (Direct Cost: ¥35,500,000、Indirect Cost: ¥10,650,000)
Fiscal Year 2006: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2005: ¥12,350,000 (Direct Cost: ¥9,500,000、Indirect Cost: ¥2,850,000)
Fiscal Year 2004: ¥12,480,000 (Direct Cost: ¥9,600,000、Indirect Cost: ¥2,880,000)
Fiscal Year 2003: ¥14,820,000 (Direct Cost: ¥11,400,000、Indirect Cost: ¥3,420,000)
Keywordstick / cDNA library / Tick bioactive molecules / TBM / baculovirus / recombinant / bioacaricide / anti-tick vaccine
Research Abstract

The objective of this research is to develop bioacaricides and /or novel vaccines for tick control that do not pose environmental and health risks associated with chemical residues and can be used as an attractive alternative to chemical pesticides. In order to realize this approach, we must clarify tick bioactive molecules (TBMs) that are responsible for the blood-sucking and innate immunity in ticks and identify as the bioacaricide and/or vaccine candidates, because blood-sucking and innate immunity are important features of the hard ticks essential for survival, reproduction and pathogen transmission. Biochemical and functional analysis of these TBMs may therefore represent interesting target molecules to which novel and environmentally safety tick control could be directed.
(1) During the period of this project, we could carry out about 50 novel TBMs the molecular identification and characterization researches on about 50 novel TBMs of the ixodid tick, Haemaphysalis longicornis.
(2) We have tested a recombinant baculovirus expressing a chitinase gene from H. longicornis for its efficacy as a tick bioacaricide. A mixture of recombinant virus and chitinase was found to kill ticks faster than pure chitinase and recombinant virus alone.
(3) We have examined the vaccine efficacy of two recombinant cement-like proteins, hlim2 and hlim3 against mice. The complementary effects of hlim2 on tick engorged body weight and hlim3 on tick attachment indicate the suitability of the 2 recombinant proteins for use in a cocktail vaccine.
(4) We have immunized rabbits with a glycine-rich protein, RH50, from the tick Rhipicephalus haemaphysaloides and evaluated its vaccine potential against tick feeding. The results showed that the RH50 protein could be useful candidate for anti-tick vaccine because the low attachment rate both in the adult and nymphal ticks, and the high mortality of nymphal ticks feeding on recombinant RH50 immunized rabbits were found.

Report

(5 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • 2004 Annual Research Report
  • 2003 Annual Research Report
  • Research Products

    (33 results)

All 2007 2006 2005 2004 Other

All Journal Article (25 results) Patent(Industrial Property Rights) (2 results) Publications (6 results)

  • [Journal Article] Babesia parasites develop and are transmitted by the non-vector soft tick Ornithodoros moubata (Acari : Argasidae).2007

    • Author(s)
      Battsetseg B
    • Journal Title

      Parasitology 134(1)

      Pages: 1-8

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Haemaphysalis longicornis : Molecular characterization of a homologue of the macrophage migration inhibitory factor from the partially fed ticks.2007

    • Author(s)
      Umemiya R
    • Journal Title

      Exp. Parasitol. 115(1)

      Pages: 135-142

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Babesia parasites develop and are transmitted by the non-vector soft tick Ornithodoros moubata (Acari : Argasidae).2007

    • Author(s)
      Battsetseg B et al.
    • Journal Title

      Parasitology 134(1)

      Pages: 1-8

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Haemaphysalis longicornis : Molecular characterization of a homologue of the macrophage migration inhibitory factor from the partially fed ticks.2007

    • Author(s)
      Umemiya R et al.
    • Journal Title

      Exp. Parasitol. 115

      Pages: 135-142

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Identification and characterization of a leucine aminopeptidase from the hard tick Haemaphysalis longicornis.2006

    • Author(s)
      Hatta T
    • Journal Title

      Int. J. Parasitol. 36(10-11)

      Pages: 1123-1132

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] A secreted cystatin from the tick Haemaphysalis longicornis and its distinct expression patterns in relation to innate immunity.2006

    • Author(s)
      Zhou J
    • Journal Title

      Insect Biochem. Mol. Biol. 36(7)

      Pages: 527-535

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Tick troponin I-like molecule is a potent inhibitor for angiogenesis.2006

    • Author(s)
      Fukumoto S
    • Journal Title

      Microvasc. Res. 71(3)

      Pages: 218-221

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Identification of a follistatin-related protein from the tick Haemaphysalis longicornis and its effect on tick oviposition.2006

    • Author(s)
      Zhou J
    • Journal Title

      Gene 372

      Pages: 191-198

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Identification and characterization of a leucine aminopeptidase from the hard tick Haemaphysalis longicornis.2006

    • Author(s)
      Hatta T et al.
    • Journal Title

      nt. J. Parasitol. 36

      Pages: 1123-1132

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] A secreted cystatin from the tick Haemaphysalis longicornis and its distinct expression patterns in relation to innate immunity.2006

    • Author(s)
      Zhou J et al.
    • Journal Title

      Insect Biochem. Mol. Biol. 36

      Pages: 527-535

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Tick troponin Hike molecule is a potent inhibitor for angiogenesis.2006

    • Author(s)
      Fukumoto S et al.
    • Journal Title

      Microvasc. Res. 71

      Pages: 218-221

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Identification of a follistatin-related protein from the tick Haemaphysalis longicornis and its effect on tick oviposition.2006

    • Author(s)
      Zhou J et al.
    • Journal Title

      Gene 372

      Pages: 191-198

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Identification and characterization of a leucine aminopeptidase from the hard tick Haemaphysalis longicornis.2006

    • Author(s)
      Hatta T
    • Journal Title

      Int. J.Parasitol. 36(10-11)

      Pages: 1123-1132

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Tick troponin I-like molecule is a potent inhibitor for angiogenesis.2006

    • Author(s)
      Fukumoto F
    • Journal Title

      Microvasc. Res. 71(3)

      Pages: 218-221

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Molecular cloning and functional characterization of an aspartic protease from the hard tick Haemaphysalis longicornis2006

    • Author(s)
      Boldbaatar D
    • Journal Title

      Insect Biochem.Mol.Biol. 36(1)

      Pages: 25-36

    • Related Report
      2005 Annual Research Report
  • [Journal Article] The use of a recombinat baculovirus expressing a chitinase from the hard tick Haemaphysalis longicornis and its potential application as a bioacaricide for tick control.2006

    • Author(s)
      Assenga SP
    • Journal Title

      Parasitol.Res. 98(2)

      Pages: 111-118

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Identification of a follistatin-related protein from the tick Haemaphysalis longicornis and its effect on tick oviposition.2006

    • Author(s)
      Zhou J
    • Journal Title

      Gene 371(in press.)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Tick troponin I-like molecule is a potent inhibitor for angiogenesis2006

    • Author(s)
      Fukumoto S
    • Journal Title

      Microvasc.Res. (in press)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Epidemiological study of equine piroplasmosis in Mongolia2005

    • Author(s)
      Boldbaatar, D.
    • Journal Title

      Vet.Parasitol. 127(1)

      Pages: 29-32

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Epidemiological study of equine piroplasmosis in Mongolia.2005

    • Author(s)
      Boldbaatar, D.
    • Journal Title

      Vet.Parasitol. 127(1)

      Pages: 35-38

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Tickcidal effect of monoclonal antibodies against hemocytes, Om21, in an adult female tick, Ornithodoros moubata (Acari Argasidae).2004

    • Author(s)
      Matsuo, T.
    • Journal Title

      J.Parasitol. 90(4)

      Pages: 715-720

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Cloning and molecular characterization of a cubilin-related serine protease from the hard tick Haemaphysalis longicornis.2004

    • Author(s)
      Miyoshi, T.
    • Journal Title

      Insect Biochem.Mol.Biol. 34(8)

      Pages: 799-808

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Fate of GFP-expressed Escherichia coli in the midgut and reaction of hemocytes in a tick, Ornithodoros moubata (Acari : Argasidae).2004

    • Author(s)
      Matsuo, T.
    • Journal Title

      Exp.Parasitol. 108(1-2)

      Pages: 67-73

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Enzymatic characterization of a cubilin-related serine proteinase from the hard tick Haemaphysalis longicornis.2004

    • Author(s)
      Miyoshi, T.
    • Journal Title

      J.Vet.Med.Sci. 66(10)

      Pages: 1195-1198

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Gene scielncing of a cubilin-related serine proteinase from the hard tick Haemaphysalis longicornis.2004

    • Author(s)
      Miyoshi, T.
    • Journal Title

      J.Vet.Med.Sci. 66(11)

      Pages: 1471-1473

    • Related Report
      2004 Annual Research Report
  • [Patent(Industrial Property Rights)] マダニのアスパラギン酸プロテアーゼ2005

    • Inventor(s)
      藤崎 幸蔵, 玄 学南, Boldbaatar D
    • Industrial Property Rights Holder
      帯広畜産大学
    • Filing Date
      2005-05-30
    • Related Report
      2005 Annual Research Report
  • [Patent(Industrial Property Rights)] ベクターマダニのガレクチン、それをコードする核酸分子及びそれらの利用2005

    • Inventor(s)
      辻 尚利, 黄 暁紅, 三好 猛晴, 藤崎 幸蔵
    • Industrial Property Rights Holder
      農業研究機構, 帯広畜産大学
    • Filing Date
      2005-08-03
    • Related Report
      2005 Annual Research Report
  • [Publications] You, M.: "Molecular characterization of a chitinase protein from the hard tick Haemaphysalis longicornis"J.Biol.Chem.. 278・10. 8556-8563 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Matsuo, T.: "Morphological studies on the extracellular structure of the midgut of a tick, Haemaphysalis longicornis(Acari : Ixodidae)"Parasitol.Res.. 90・3. 243-248 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Matsuo, T.: "Development, characterization and tickcidal effect of monoclonal antibodies against hemocytes in an adult female tick, Ornithodoros moubata (Acari : Argasidae)"Vet.Parasitol.. 118・1-2. 109-119 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kadota, K.: "Morphological and populational characteristics of hemocytes of Ornithodoros moubata nymphs during the ecdysial phase"J.Med.Entomol.. 40・6. 770-776 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Matsuo, T.: "Tickcidal effect of monoclonal antibodies against hemocytes, Om21, in an adult female tick, Ornithodoros moubata(Acari Argasidae)"J.Parasitol.. (印刷中). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Ruheta, M.R.: "Advances in the artificial feeding of Ornithodoros moubata(Acari : Ixodiae) and the follow up its life cycle after feeding on fetal bovine serum"J.Protzool.Res.. (印刷中). (2004)

    • Related Report
      2003 Annual Research Report

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Published: 2003-04-01   Modified: 2016-04-21  

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