• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Roles of multifunctional adaptor proteins, GGAs, in membrane traffic

Research Project

Project/Area Number 15209002
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Biological pharmacy
Research InstitutionKyoto University

Principal Investigator

NAKAYAMA Kazuhisa  Kyoto University, Graduate School of Pharmaceutical Sciences, Professor, 薬学研究科, 教授 (40192679)

Co-Investigator(Kenkyū-buntansha) SHIN Hye-Won  Kyoto University, Graduate School of Pharmaceutical Sciences, Research associate, 薬学研究科, 研究員(科学研究) (10345598)
Project Period (FY) 2003 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥50,440,000 (Direct Cost: ¥38,800,000、Indirect Cost: ¥11,640,000)
Fiscal Year 2005: ¥8,450,000 (Direct Cost: ¥6,500,000、Indirect Cost: ¥1,950,000)
Fiscal Year 2004: ¥18,330,000 (Direct Cost: ¥14,100,000、Indirect Cost: ¥4,230,000)
Fiscal Year 2003: ¥23,660,000 (Direct Cost: ¥18,200,000、Indirect Cost: ¥5,460,000)
KeywordsCells and tissues / Biomolecules / Proteins / Pharmaceutical sciences / GGA / GATドメイン / ユビキチン / 小胞輸送 / X線結晶構造解析 / Tom1 / Tollip
Research Abstract

In this research project, we revealed the followings on GGAs and its related Tom1 family proteins.
(1)We found that ARFs bind to the N-GAT subdomain of the GAT domain of GGAs.
(2)By X-ray crystallography, we revealed the three-dimensional structure of the GGA3-GAT domain in complex with ARF.
(3)We found that ubiquitin bind to the C-GAT subdomain of the GAT domain of GGAs and Tom1 family proteins.
(4)By X-ray crystallography, we revealed the three-dimensional structure of the GGA3-GAT domain and that of the Tom1-GAT domain in complex with ubiquitin.
(5)We found that Tollip (Toll-interacting protein) interacts with the GAT domain of the Tom1 family proteins.
(6)We revealed that Tollip recruits Tom1 family proteins and ubiquitinated proteins onto endosomes.

Report

(4 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • 2003 Annual Research Report
  • Research Products

    (48 results)

All 2006 2005 2004 2003 Other

All Journal Article (41 results) Book (2 results) Publications (5 results)

  • [Journal Article] Recruitment of clathrin onto endosomes by the Tom1-Tollip complex.2006

    • Author(s)
      Katoh, Y. et al.
    • Journal Title

      Biochem. Biophys. Res. Commun. 341

      Pages: 143-149

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Recruitment of clathrin onto endosomes by the Tom1-Tollip complex.2006

    • Author(s)
      Katoh, Y. et al.
    • Journal Title

      Biochem.Biophys.Res.Commun. 341

      Pages: 143-149

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Recruitment of clathrin onto endosomes by the Tom1-Tollip complex.2006

    • Author(s)
      Katoh, Y., Imakagura, H., Futatsumori, M., Nakayama, K.
    • Journal Title

      Biochem.Biophys.Res.Commun. 341

      Pages: 143-149

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Structural basis for recognition of ubiquitinated cargo by Tom1-GAT domain.2005

    • Author(s)
      Akutsu, M. et al.
    • Journal Title

      FEBS Lett. 579

      Pages: 5385-5391

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Roles of ARFRP1 (ADP-ribosylation factor-related protein1)in post-Golgi membarane trafficking.2005

    • Author(s)
      Shin, H.-W.et al.
    • Journal Title

      J. Cell Sci. 118

      Pages: 4039-4048

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Molecular mechanism of ubiquitin recognition by GGA3 GAT domain.2005

    • Author(s)
      Kawasaki, M. et al.
    • Journal Title

      Genes Cells 10

      Pages: 639-654

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Cellular and subcellular localization of EFA6C, a third member of the EFA6 family, in adult mouse Purkinje cells.2005

    • Author(s)
      Matsuya, S. et al.
    • Journal Title

      J. Neurochem. 93

      Pages: 674-685

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Convergence of cell cycle regulation and growth factor signals on GRASP65.2005

    • Author(s)
      Yoshimura, S. et al.
    • Journal Title

      J. Biol. Chem. 280

      Pages: 23048-23056

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Roles of ARFRP1 (ADP-ribosylation factor-related protein 1) in post-Golgi membrane trafficking.2005

    • Author(s)
      Shin, H.-W.et al.
    • Journal Title

      J.Cell Sci. 118

      Pages: 4039-4048

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Cellular and subcellular localization of EFA6C, a third member of the EFA6 family, in adult mouse Purkinje cells.2005

    • Author(s)
      Matsuya, S. et al.
    • Journal Title

      J.Neurochem. 93

      Pages: 674-685

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Convergence of cell cycle regulation and growth factor signals on GRASP652005

    • Author(s)
      Yoshimura, S. et al.
    • Journal Title

      J.Biol.Chem. 280

      Pages: 23048-23056

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Analysis of ARF interaction with GGAs in vitro and in vivo.2005

    • Author(s)
      Nakayama, K., Takatsu, H.
    • Journal Title

      Methods in Enzymology, GTPases regulating membrane dynamics (Balch, W.E., Der, C.J. & Hall, A., eds.)(Elsevier) Vol.404

      Pages: 367-377

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Expression of BIG2 and analysis of its function in Mammalian Cells.2005

    • Author(s)
      Shin, H.-W., Shinotsuka, C., Nakayama, K.
    • Journal Title

      Methods in Enzymology, GTPases regulating membrane dynamics (Balch, W.E., Der, C.J. & Hall, A., eds.)(Elsevier) Vol.404

      Pages: 206-215

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Structural basis for recognition of ubiquitinated cargo by Tom1-GAT domain.2005

    • Author(s)
      Akutsu, M., Kawasaki, M., Katoh, Y., Shiba, T., Yamaguchi, Y., Kato, R., Kato, K., Nakayama, K., Wakatsuki, S.
    • Journal Title

      FEBS Lett. 579

      Pages: 5385-5391

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Roles of ARFRP1(ADP-ribosylation factor-related protein 1) in post-Golgi membrane trafficking.2005

    • Author(s)
      Shin, H.-W., Kobayashi, H., Kitamura, M., Waguri, S., Suganuma, T., Uchiyama, Y., Nakayama, K.
    • Journal Title

      J.Cell Sci. 118

      Pages: 4039-4048

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Molecular mechanism of ubiquitin recognition by GGA3 GAT domain.2005

    • Author(s)
      Kawasaki, M., Shiba, T., Shiba, Y., Yamaguchi, Y., Matsugaki, N., Igarashi, N., Suzuki, M., Kato, R., Kato, K., Nakayama, K., Wakatsuki, S.
    • Journal Title

      Genes Cells 10

      Pages: 639-654

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Structure determination of GGA-GAE and gl-ear in complex with peptides : crystallization of low-affinity complexes in membrane traffic.2005

    • Author(s)
      Yamada, Y., Inoue, M., Shiba, T., Kawasaki, M., Kato, R., Nakayama, K., Wakatsuki, S.
    • Journal Title

      Acta.Cryst.D. 61

      Pages: 731-736

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Cellular and subcellular localization of EFA6C, a third member of the EFA6 family, in adult mouse Purkinje cells.2005

    • Author(s)
      Matsuya, S., Sakagami, H., Tohgo, A., Owada, Y., Shin, H.-W., Takeshima, H., Nakayama, K., Kokubun, S., Kondo, H.
    • Journal Title

      J.Neurochem. 93

      Pages: 674-685

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Convergence of cell cycle regulation and growth factor signals on GRASP65.2005

    • Author(s)
      Yoshimura, S., Yoshioka, K., Barr, F.A., Lowe, M., Nakayama, K., Ohkuma, S., Nakamura, N.
    • Journal Title

      J.Biol.Chem. 280

      Pages: 23048-23056

    • Related Report
      2005 Annual Research Report
  • [Journal Article] メンブレントラフィックを調節する新規のクラスリンアダプターの構造と機能2005

    • Author(s)
      中山和久, 若槻壮市
    • Journal Title

      生化学 78

      Pages: 1367-1381

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Membrane recruitment of effector proteins by Arf and Rab GTPases.2005

    • Author(s)
      Kawasaki, M., Nakayama, K., Wakatsuki, S.
    • Journal Title

      Curr.Opin.Struct.Biol. 15

      Pages: 681-689

    • Related Report
      2005 Annual Research Report
  • [Journal Article] BIG2, a Guanine Nucleotide Exchange Factor for ADP-Ribosylation Factors : Its Localization to Recycling Endosomes and Implication in the Endosome Integrity.2004

    • Author(s)
      Shin, H.-W.et al.
    • Journal Title

      Mol. Biol. Cell 15

      Pages: 5283-5294

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Stabilin-1 localises to endosomes and the trans-Golgi network in human macrophages and interacts with GGA adaptors.2004

    • Author(s)
      Kzhyshkowska, J. et al.
    • Journal Title

      J. Leukocyte Biol. 76

      Pages: 1151-1161

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Tollip and Tom1 form a Complex and recruit ubiquitin-conjugated proteins onto early endosomes.2004

    • Author(s)
      Katoh, Y. et al.
    • Journal Title

      J. Biol. Chem. 279

      Pages: 24435-24443

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] GAT (GGA and Tom1) domain responsible for ubiquitin binding and ubiquitination.2004

    • Author(s)
      Shiba, Y. et al.
    • Journal Title

      J. Biol. Chem. 279

      Pages: 7105-7111

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] BIG2, a Guanine Nucleotide Exchange Factor for ADP-Ribosylation Factors : Its Localization to Recycling Endosomes and Implication in the Endosome Integrity.2004

    • Author(s)
      Shin, H.-W.et al.
    • Journal Title

      Mol.Biol.Cell 15

      Pages: 5283-5294

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Stabilin-1 localises to endosomes and the trans-Golgi network in human macrophages and interacts with GGA adaptors.2004

    • Author(s)
      Kzhyshkowska, J
    • Journal Title

      J.Leukocyte Biol. 76

      Pages: 1151-1161

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Tollip and Tom1 form a Complex and recruit ubiquitin-conjugated proteins onto early endosomes2004

    • Author(s)
      Katoh, Y
    • Journal Title

      J.Biol.Chem. 279

      Pages: 24435-24443

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] GAT (GGA and Tom1) domain responsible for ubiquitin binding and ubiquitination.2004

    • Author(s)
      Shiba, Y
    • Journal Title

      J.Biol.Chem. 279

      Pages: 7105-7111

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Tollip and Tom1 form a complex and recruit ubiquitin-conjugated proteins onto early endosomes.2004

    • Author(s)
      Katoh, Y., et al.
    • Journal Title

      J.Biol.Chem. 279

      Pages: 24435-24443

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Stabilin-1 localises to endosomes and the train-Golgi network in human macrophages and interacts with GGA adaptors.2004

    • Author(s)
      Kzhyshkowska, J., et al.
    • Journal Title

      J.Leukocyte Biol. 76

      Pages: 1151-1161

    • Related Report
      2004 Annual Research Report
  • [Journal Article] BIG2, a guanine nucleotide exchange factor for ADP- ribosylation factors : its localization to recycling endosomes and implication in the endosome integrity.2004

    • Author(s)
      Shin, H.-W., et al.
    • Journal Title

      Mol.Biol.Cell 15

      Pages: 5283-5294

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Guanine nucleotide exchange factors for Arf GTPases : their diverse functions in membrane traffic.2004

    • Author(s)
      Shin, H.-W., Nakayama, K.
    • Journal Title

      J.Biochem. 136

      Pages: 761-767

    • NAID

      10016204135

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Identification of a five-pass transmembrane protein family localizing in the Golgi apparatus and the ER.2003

    • Author(s)
      Shakoori, A. et al.
    • Journal Title

      Biochem. Biophys. Res. Commun. 312

      Pages: 850-857

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Role of ADP-ribosylation factor 6 (ARF6) in gastric acid secretion.2003

    • Author(s)
      Matsukawa, J. et al.
    • Journal Title

      J.BIol. Chem. 278

      Pages: 36470-36475

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Predominant expression of the short form of GGA3 in human cell lines and tissues.2003

    • Author(s)
      Wakasugi, M. et al.
    • Journal Title

      Biochem. Biophys. Res. Commun. 306

      Pages: 687-692

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Molecular mechanism of membrane recruitment of GGA by ARF in lysosomal protein transport.2003

    • Author(s)
      Shiba, T. et al.
    • Journal Title

      Nature Struct. Biol. 10

      Pages: 386-393

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Identification of a five-pass transmembrane protein family localizing in the Golgi apparatus and the ER.2003

    • Author(s)
      Shakoori, A.
    • Journal Title

      Biochem.Biophys.Res.Commun. 312

      Pages: 850-857

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Role of ADP-ribosylation factor 6 (ARF6) in gastric acid secretion.2003

    • Author(s)
      Matsukawa, J. et al.
    • Journal Title

      J.Biol.Chem. 278

      Pages: 36470-36475

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Predominant expression of the short form of GGA3 in human cell lines and tissues.2003

    • Author(s)
      Wakasugi, M. et al.
    • Journal Title

      Biochem.Biophys.Res.Commun. 306

      Pages: 687-692

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Molecular mechanism of membrane recruitment of GGA by ARF in lysosomal protein transport.2003

    • Author(s)
      Shiba, T. et al.
    • Journal Title

      Nature Struct.Biol. 10

      Pages: 386-393

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Book] Analysis of ARF interaction with GGAs in vitro and in vivo. In Methods in Enzymology, Vol. 404, GTPases regulating membrane dynamics2005

    • Author(s)
      Nakayama, K. et al.
    • Total Pages
      692
    • Publisher
      Elsevier
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Book] Expression of BIG2 and analysis of its function in Mammalian Cells. In Methods in Enzymology, Vol. 404, GTPases regulating membrane dynamics2005

    • Author(s)
      Shin, H.-W.et al.
    • Total Pages
      692
    • Publisher
      Elsevier
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Publications] Shiba, Y., et al.: "GAT(GGA and Tom1) domain responsible for ubiquitin binding and ubiquitination"J.Biol.Chem.. 279. 7105-7111 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Shakoori, A., et al.: "Identification of a five-pass transmembrane protein family localizing in the Golgi apparatus and the ER."Biochem.Biophys.Res.Commun.. 312. 850-857 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Matsukawa, J., et al.: "Role of ADP-ribosylation factor 6(ARF6) in gastric acid secretion"J.Biol.Chem.. 278. 36470-36475 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Wakasugi, M., et al.: "Predominant expression of the short form of GGA3 in human cell lines and tissues"Biochem.Biophys.Res.Commun.. 306. 687-692 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Shiba, T., et al.: "Molecular mechanism of membrane recruitment of GGA by ARF in lysosomal protein transport"Nat.Struct. Biol.. 10. 368-393 (2003)

    • Related Report
      2003 Annual Research Report

URL: 

Published: 2003-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi