| Project/Area Number |
15209011
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General medical chemistry
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| Research Institution | University of Tokyo |
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Principal Investigator |
SEIKI Motoharu The University of Tokyo, Institute of Medical Science, Professor, 医科学研究所, 教授 (10154634)
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| Co-Investigator(Kenkyū-buntansha) |
YANA Ikuo The University of Tokyo, Institute of Medical Science, Lecturer, 医科学研究所, 講師 (70332583)
IZUMI Tomonori The University of Tokyo, Institute of Medical Science, Research Scientist(Donation Laboratory), 医科学研究所, 寄付研究部門教員(常勤形態) (00261694)
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| Project Period (FY) |
2003 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥49,920,000 (Direct Cost: ¥38,400,000、Indirect Cost: ¥11,520,000)
Fiscal Year 2005: ¥16,250,000 (Direct Cost: ¥12,500,000、Indirect Cost: ¥3,750,000)
Fiscal Year 2004: ¥16,250,000 (Direct Cost: ¥12,500,000、Indirect Cost: ¥3,750,000)
Fiscal Year 2003: ¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
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| Keywords | Membrane-type MMP / Integrins / Menbrane proteins / Proteomic analysis / MT1-MMP / 浸潤装置 / がん細胞 / プロテアーゼ / 細胞接着分子 / Matrix metalloproteinase / Proteolysis / Membrane proteins / proteomics / nano-LC-MS / MS |
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| Research Abstract |
We established a transfected human squemous carcinoma A431 cell line m which expression of FLAG-tagged MT1-MMP or FIAG-tagged integrins beta-1 is inducible. We set up conditions to isolate the FLAG-tagged MT1-MMP including various associating molecules using different types of detergents. We performed systemic analysis of the proteins in the complex. Five secreted proteins, 64 membrane proteins, 87 cytoplasmic proteins, and 7 unknown proteins were identified Then, we examined 18 membrane proteins whether they can be substrates of MT1-MMP by expressing them in COS-1 cells. Nine of them were found to be cleaved by the expression of MT1-MMP generating processed fragments. One of the processed proteins has been reported to be overexpressed many tumors and be shed by metalloproteinases. We confirmed that this protein also cleaved by endogenous MT1-MMP in human tumor cell lines that express MT1-MMP. Thus, we believe this approach is useful to identify MT1-MMP substrates and regulators by associating with MT1-MMP We are trying to know the functions of the substrates and associating proteins by the knock-down experiments using siRNA. The association of the identified proteins with MT1-MMP is further tested using cross-linkers. We are continuing analysis of integrin-assoaating proteins as well.
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