| Project/Area Number |
15209019
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (A)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Applied pharmacology
|
|---|
| Research Institution | Tokyo Institute of Technology |
|---|
Principal Investigator |
ABE Masanori Tokyo Institute of Technology, Department of Physical Electronics, Professor, 大学院・理工学研究科, 教授 (70016624)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
HANDA Hiroshi Tokyo Institute of Technology, Department of Biologiral Information Science, Professor, 大学院・生命理工学研究科, 教授 (80107432)
HIBI Norifumi Keio University, Department of Internal Medicine, Professor, 医学部, 教授 (50129623)
|
|---|
| Project Period (FY) |
2003 – 2005
|
| Project Status |
Completed (Fiscal Year 2005)
|
| Budget Amount *help |
¥50,700,000 (Direct Cost: ¥39,000,000、Indirect Cost: ¥11,700,000)
Fiscal Year 2005: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000)
Fiscal Year 2004: ¥18,330,000 (Direct Cost: ¥14,100,000、Indirect Cost: ¥4,230,000)
Fiscal Year 2003: ¥23,790,000 (Direct Cost: ¥18,300,000、Indirect Cost: ¥5,490,000)
|
|---|
| Keywords | Magnetic beads / Bio-screening / Bio-sensing / MRI contrast agent / Hyperthermia / Anticancer drug / DNA / Ferrite nanobeads / 磁性ビーズ / DNA検出 / MRI造影剤 / フェライト粒子 / GMA / 抗体 / MMI造影 / フェライトナノビーズ / IgG抗体 / CEA抗体 |
|---|
| Research Abstract |
1. Development of size controlled ferrite nanobeads
Iron-ferrite nanoparticles with controlled size of 10-100 nm were successfully synthesized by our newly developed methods called co-precipitation, partial-oxidation, and seed-growth methods.
2. Development of functional-polymer coated ferrite nanobeads
The iron-ferrite nanoparticles were successfully coated with poly-GMA, which exhibits minimal non-specific adsorption of proteins, by a co-polymerization method and a novel, soap-free polymerization method.
3. Development of polymer coated iron nanobeads
Metallic iron nanoparticles (7-20 nm in size), which have magnetization two times stronger than that of ferrite particles and are advantageous for magnetic separation in bio-screening, were successfully coated with poly-GMA.
4. Application to analysis by bio-screening system
The poly-GMA coated ferrite nanobeads were conjugated with physiologically active substances such as anticancer antibodies and recombinant proteins containing tagged Prote
… More
in-G. Utilizing the beads, we successfully captured physiologically active substances that are complementary to those conjugated onto the beads. This revealed our beads are fitted for magnetic carriers for high throughput bio-screening.
5. Application to diagnosis by Hall bio-sensing
poly-GMA coated ferrite nanobeads were conjugated with single strand DNA, which were immobilized onto the surface of a Hall sensor via hybridization with the complementary DNA single strand fixed on the sensor surface. This revealed our beads are applicable to magnetic carriers for DNA analysis.
6. Application to diagnosis by contrast enhanced MRI
We successfully modified the surfaces of the ferrite nanobeads with a variety of functional molecules, and discovered candidate materials for high performance MRI contrast agent.
7. Application to anticancer magnetic hyperthermia
Utilizing the ferrite nanobeads as heat source by magnetic-field induction at an non-invasive frequency of 120 kHz, we successfully killed human colon cancer cells. We thus revealed that our beads are applicable to magnetic carriers for non-invasive, anticancer magnetic hyperthermia. Less
|
