| Project/Area Number |
15209043
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Kobe University |
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Principal Investigator |
KURODA Yoshikazu Kobe University, Graduate School of Medicine, Professor, 大学院・医学系研究科, 教授 (70178143)
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| Co-Investigator(Kenkyū-buntansha) |
SUZUKI Yasuyuki Kobe University, Graduate School of Medicine, Assosiate Professor, 大学院・医学系研究科, 助教授 (40304092)
FUJINO Yasuhiro Kobe University, Hospital, Assosiate Professor, 医学部附属病院, 講師 (30335450)
TANIOKA Yasuki Kobe University, COE Researcher, 大学院・医学系研究科, COE研究員 (00372649)
YOKONO Koichi Kobe University, Graduate School of Medicine, Professor, 大学院・医学系研究科, 教授 (50144580)
谷口 洋 神戸大学, 医学部, 教授 (70030989)
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| Project Period (FY) |
2003 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥47,970,000 (Direct Cost: ¥36,900,000、Indirect Cost: ¥11,070,000)
Fiscal Year 2005: ¥14,560,000 (Direct Cost: ¥11,200,000、Indirect Cost: ¥3,360,000)
Fiscal Year 2004: ¥10,660,000 (Direct Cost: ¥8,200,000、Indirect Cost: ¥2,460,000)
Fiscal Year 2003: ¥22,750,000 (Direct Cost: ¥17,500,000、Indirect Cost: ¥5,250,000)
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| Keywords | islet translation / non-heart beating donor / warm ischemic time / two-layer method / islet culture |
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| Research Abstract |
Our institute was approved as an islet transplantation center of west Kinki and Chugoku area at a meeting of the islet transplant working group in March 2006. We started our islet transplant program as follows.
We send our special team for organ procurement from a non-heart-beating donor and preserve a human pancreas by the two-layer method during transportation. We isolate human islets from the pancreas by the Ricordi method and examine islet recovery. We perform morphological and functional assessments of human islets after the isolation process and after one-day culture of islets by the two-layer method. When the islet recover is over 5000 IE/kg of a recipient weight, we perform islet transplantation to a patient of type 1 diabetes.
By animal experiments, we certified and reported improvement of islet recovery and function by applying the two-layer method for pancreas preservation prior to islet isolation. And furthermore we investigated usefulness of the two-layer method for islet cu
… More
lture. The above experiments show importance of the two-step two-layer method for islet transplantation.
In 2005 we performed three cases of human islet isolation. From one of the three cases, we performed islet transplantation to a recipient of type 1 diabetes. Donor was 32 year old female, warm ischemia time was 6min, cold ischemic time was 193min, and preservation period by the two-layer method was 165min. Pre purification recovery was 545267 IEQ, and post purification recovery was 483545 IEQ. This was up to the standard of islet transplantation. The recipient was a 30-year old female of unstable type 1 diabetes. We can detect endogenous insulin release in the recipient after islet transplantation. The recipient was dissolved unstableness of blood glucose level.
In 2004, we confirmed superiority of the two-step two-layer method. In 2005, we showed the possibility of islet transplantation from the pancreas damaged by warm ischemia. Clinically we reported a valuable case of successful human islet transplantation from a young, low BMI, non-heart-beating cadaver. Less
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