| Project/Area Number |
15209050
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (A)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Orthopaedic surgery
|
|---|
| Research Institution | Osaka University |
|---|
Principal Investigator |
YOSHIKAWA Hideki Osaka University, Graduate School of Medicine, Professor, 医学系研究科, 教授 (60191558)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
SUGANO Nobuhiko Osaka University, Graduate School of Medicine, Associate Professor, 医学系研究科, 助教授 (70273620)
MYUOI Akira Osaka University, Hospital, Associate Professor, 医学部附属病院, 助教授 (10263261)
TSUMAKI Noriyuki Osaka University, Graduate School of Medicine, Assistant, 医学系研究科, 助手 (50303938)
|
|---|
| Project Period (FY) |
2003 – 2006
|
| Project Status |
Completed (Fiscal Year 2006)
|
| Budget Amount *help |
¥42,380,000 (Direct Cost: ¥32,600,000、Indirect Cost: ¥9,780,000)
Fiscal Year 2006: ¥7,670,000 (Direct Cost: ¥5,900,000、Indirect Cost: ¥1,770,000)
Fiscal Year 2005: ¥9,620,000 (Direct Cost: ¥7,400,000、Indirect Cost: ¥2,220,000)
Fiscal Year 2004: ¥11,570,000 (Direct Cost: ¥8,900,000、Indirect Cost: ¥2,670,000)
Fiscal Year 2003: ¥13,520,000 (Direct Cost: ¥10,400,000、Indirect Cost: ¥3,120,000)
|
|---|
| Keywords | bone regeneration / bone substitute / mesenchymal stem cell / osteoblast / tissue engineering / hydroxyapatite / 分化誘導 |
|---|
| Research Abstract |
We developed a fully interconnected porous hydroxyapatite (IP-CHA) by adopting the "form-gel" technique. The interconnected porous structure facilitates tissue engineering by allowing the introduction of bone cells, rhBMP-2, or vasculature into the pores. IP-CHA combined with rat marrow mesenchymal cells showed abundant bone formation inside the pore areas. IP-CHA combined with recombinant human bone morphogenetic protein-2 (rhBMP-2) completely repaired the bony defect of rabbit radius. IP-CHA/BMP composite with insertion of epigastric vessels exhibited abundant new bone formation in the pores of the deep portion close to the inserted vessels. The 3-dimensional fully interconnected porous structure of IP-CHA encourages bone ingrowth in the material and eventually leads to good incorporation of the material into host bone. The synthetic scaffold can be prefabricated into specific sizes and shapes to match bone defects, and even into composite with solid form of HA in order to reinforce its initial mechanical strength.
|
