| Project/Area Number |
15209053
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Kyoto University, Graduate School of Medicine |
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Principal Investigator |
FUJII Shingo Kyoto Univ.Med, Gynecol & Obstet., Professor, 医学研究科, 教授 (30135579)
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| Co-Investigator(Kenkyū-buntansha) |
FUJITA Jun Kyoto Univ.Med, Professor, 医学研究科, 教授 (50173430)
TOGASHI Kaori Kyoto Univ.Med, Professor, 医学研究科, 教授 (90135484)
TAKAKURA Ken Kyoto Univ.Med, Associate Professor, 医学研究科, 助教授 (10221350)
KARIYA Masatoshi Kyoto Univ.Med, Associate Professor, 医学研究科, 講師 (90243013)
NIKAIDO Toshio Toyama Univ.Med, Professor, 教授 (50180568)
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| Project Period (FY) |
2003 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥50,050,000 (Direct Cost: ¥38,500,000、Indirect Cost: ¥11,550,000)
Fiscal Year 2005: ¥14,040,000 (Direct Cost: ¥10,800,000、Indirect Cost: ¥3,240,000)
Fiscal Year 2004: ¥13,910,000 (Direct Cost: ¥10,700,000、Indirect Cost: ¥3,210,000)
Fiscal Year 2003: ¥22,100,000 (Direct Cost: ¥17,000,000、Indirect Cost: ¥5,100,000)
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| Keywords | uterine smooth muscle tumor / oxidative stress / re-perfusion / gray zone / p53 / microarray / 月経 / 子宮収縮 / ストレス / 肥満細胞 / アクログラニン / MRI / 子宮筋腫 / 子宮肉腫 / 細胞株 / Acrogranin / 性ステロイド受容体 / TGF-β / 不死化 / p53 / アポトーシス / タイプIIIコラーゲン |
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| Research Abstract |
We have investigated various biological and clinical aspects of uterine smooth muscle tumors in the past. In the current study, in view of past and ongoing insights, we tried to develop new and rational approach to handle smooth muscle tumors of the uterus. First, we set unique criteria of "gray zone" from the MR image that contains potentially malignant tumors and calls for handling with special attention. This criterion allows clinically appropriate follow up of a group of uterine smooth muscle tumors that may turn out to be malignant. Second, we further investigated our hypothesis that uterine smooth muscle tumors originate under the influence of hypoxia caused by uterine contraction in the time of menstruation. Immunostaining of p53 and p21 in normal myometrium in menstrual cycle revealed that the expression of these proteins are observed in outer layer of uterine myometrium exclusively in follicular phase of menstrual cycle, suggesting that this part of the uterus are under the hypoxic stress and prone to damage in cycle, and may leads to the development of uterine smooth muscle tumors.
Third, we have performed comparative microarray analysis among the normal uterine smooth muscle, benign leiomyomas, and sarcomas. According the result, we investigated the role of PEP-19 protein and Acrogranin in the development of uterine smooth muscle tumors, that revealed that both genes play important roles in various stages of the occurrence of them.
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