| Project/Area Number |
15300113
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Nerve anatomy/Neuropathology
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| Research Institution | Gunma University |
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Principal Investigator |
NAKAZATO Yoichi Gunma University, Graduate School of Medicine, Department of Human Pathology, Professor, 大学院・医学系研究科, 教授 (10106908)
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| Co-Investigator(Kenkyū-buntansha) |
HIRATO Junko Gunma University, Graduate School of Medicine, Associate Professor, 大学院・医学系研究科, 助教授 (60208832)
SASAKI Atsushi Gunma University, Graduate School of Medicine, Assistant Professor, 大学院・医学系研究科, 講師 (80225862)
YOKOO Hideaki Gunma University, Graduate School of Medicine, Research Associate, 大学院・医学系研究科, 助手 (40282389)
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| Project Period (FY) |
2003 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥15,000,000 (Direct Cost: ¥15,000,000)
Fiscal Year 2006: ¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 2005: ¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 2004: ¥3,900,000 (Direct Cost: ¥3,900,000)
Fiscal Year 2003: ¥4,800,000 (Direct Cost: ¥4,800,000)
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| Keywords | brain tumor / tissue microarray / image analysis / immunohistochemistry / nervous tissue specific antigen / 細織マイクロアレイ |
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| Research Abstract |
We collected well characterized cases of human brain tumor, and carried out pathological studies. First, we developed image measurement software, and measured the parameter of the morphological information automatically. The pleomorphism of a given tumor could become numerical value by the introduction of the entropy concept, and we proved that this entropy correlated with grading of the tumor.
We developed antibodies to the nervous tissues with a purpose of using for immunohistochemical study. Polyclonal antibody was made to synthesized oligopeptide of Olig2, and used for several investigations. We applied for the patent about how to make Olig2 antibody, and acquired it. The epitope recognized by Olig2 antibody was expressed in both oligodendrogliomas and astrocytomas, and moreover that expression proved to be complementary as for the GFAP expression. We investigated 6 cases of papillary glioneuronal tumor using the Olig2 antibody, and proved that oligodendrocytes also were contained in this tumor in addition to neuron and astrocytes.
We applied 37 nervous tissue associated antibodies on to tissue micro-array specimen with 378 samples of brain tumor. Immunohistochemical data were analyzed by the cluster analysis. Brain tumor was classified in seven groups as that result. We found antigens that distinguished oligodendrogliomas from astrocytomas, and also antigens that discriminated low grade glioma from high grade glioma.
The amplification of epidermal growth factor receptor gene in the glioblastoma was investigated with chromogenic in situ hybridization (CISH) method, and gene amplification was detected by 14 examples in 75 case. Intratumorale localization of gene amplified cells could be analyzed in detail with this method.
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