| Project/Area Number |
15300138
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Laboratory animal science
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| Research Institution | Hokkaido University |
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Principal Investigator |
AGUI Takashi Hokkaido University, Graduate School of Veterinary Medicine, Professor, 大学院獣医学研究科, 教授 (00212457)
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| Co-Investigator(Kenkyū-buntansha) |
ARIKAWA Jiro Hokkaido University, Graduate School of Medicine, Professor, 大学院医学研究科, 教授 (10142704)
ASANO Atsushi Hokkaido University, Graduate School of Veterinary Medicine, Assistant Professor, 大学院獣医学研究科, 助手 (90312404)
TAKAKURA Akira Central Institute for Experimental Animals, Laboratory Chief, ICLAモニタリングセンター, 室長 (60167484)
KUNIMATU Mitoshi Nagoya Women's University, Professor, 家政学部, 教授 (70145746)
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| Project Period (FY) |
2003 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥16,700,000 (Direct Cost: ¥16,700,000)
Fiscal Year 2005: ¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2004: ¥4,900,000 (Direct Cost: ¥4,900,000)
Fiscal Year 2003: ¥8,600,000 (Direct Cost: ¥8,600,000)
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| Keywords | peptide tip / antigenic epitope / hantavirus / mouse hepatitis virus / Sendai virus / Mycoplasma / マイコフラズマ |
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| Research Abstract |
We selected mouse hepatitis virus, Sendai virus, Mycoplasma plumonis, and hantavirus from the aspect of the frequency and danger upon infectious accidents. We determined antigenic epitopes by incubating polyclonal anti-sera with peptide tips produced according to the amino acid sequences reported previously elsewhere. Further, we tried to develop a preliminary ELISA kit using antigenic epitopes that we have determined. With respect to mouse hepatitis virus and Sendai virus, we succeeded to develop an ELISA system, which was more sensitive or equal to the commercially available ELISA system (paper in preparation). With respect to hantavirus, we succeeded to determine an antigenic epitope, EDVNGIRK (amino acid 166-173), to monoclonal antibody E5/G6, which recognizes nuclear protein of hantavirus. With respect to Mycoplasma plumonis, we first searched antigenic protein from the components of the bacteria, since there are a lot of proteins composing of bacteria. We found a protein showing a homology to P46 protein, which had been reported as an antigenic protein in other Mycoplasmas and named it P46L. We produced recombinant P46L protein conjugated with glutathione S-transferase and made an ELISA system with purified P46L. This ELISA system was more sensitive than the commercially available ELISA system.
We further applied this method to the determination of antigenic epitopes of West Nile virus and SARS virus and succeeded it. We furthermore succeeded to apply this method to not only determining antigenic epitopes of infectious pathogens but also determining protein-protein interaction site such as binding sits of FAS-antigen and angiotensin II.
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