• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Development of Multifunctional Envelope type Nano Device based on Programmed Packaging

Research Project

Project/Area Number 15300151
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Biomedical engineering/Biological material science
Research InstitutionHOKKAIDO UNIVERSITY

Principal Investigator

HARASHIMA Hideyoshi  Hokkaido Univ., Grad.School of Pharm.Sci., Prof., 大学院・薬学研究科, 教授 (00183567)

Co-Investigator(Kenkyū-buntansha) KAMIYA Hiroyuki  Hokkaido Univ., Grad.School of Pharm.Sci., Asso.Prof., 大学院・薬学研究科, 助教授 (10204629)
AKITA Hidetaka  Hokkaido Univ., Grad.School of Pharm.Sci., Assist., 大学院・薬学研究科, 助手 (80344472)
Project Period (FY) 2003 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥17,300,000 (Direct Cost: ¥17,300,000)
Fiscal Year 2004: ¥6,000,000 (Direct Cost: ¥6,000,000)
Fiscal Year 2003: ¥11,300,000 (Direct Cost: ¥11,300,000)
Keywordsgene delivery system / endocytosis / octaarginin / programmed packaging / MEND / アデノウイルス / 非ウイルスベクター / 定量的評価
Research Abstract

A rational strategy is required to overcome cellular and intracellular barriers and to develop an innovative non-viral gene delivery system. A new concept "Programd Packaging", for the design and assembling of intelligent nano devices into an integrated, unique non-viral gene delivery system has been developed. Based on this concept, the importance of cellular entrance for a gene delivery system, using Tf as a receptor-mediated endocytic pathway and octaarginine as a non-endocytic pathway was chosen. The advantage of a non-endocytic pathway is demonstrated by an octaarginine-modified envelope type nano device, which exerts transfection activities as high as an adenovirus. Finally, a three dimensional quantitative assay for the intracellular trafficking of a gene delivery systems and its application for the pharmacokinetic optimization of a delivery system is described. These new approaches will ultimately lead to the development of a non-viral gene delivery system which is superior to viral vectors not only from the point of view of safety, but efficiency as well.

Report

(3 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • Research Products

    (29 results)

All 2004 2003 Other

All Journal Article (17 results) Patent(Industrial Property Rights) (6 results) Publications (6 results)

  • [Journal Article] Mechanism of improved gene transfer by the N-terminal stearylation of octaarginine : Enhanced cellular association by hydrophobic core formation.2004

    • Author(s)
      I.A.Khalil
    • Journal Title

      Gene Therapy 11

      Pages: 636-644

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Quantitative three-dimesional analysis of the intracellular trafficking of plasmid DNA transfected by non-viral gene delivery system using confocal laser scanning microscopy.2004

    • Author(s)
      H.Akita
    • Journal Title

      Mol.Ther. 9

      Pages: 443-451

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Transferrin-modified liposomes equipped with pH-sensitive fusogenic peptide : An artificial viral-like delivery system2004

    • Author(s)
      T.Kakudo
    • Journal Title

      Biochemstry 18

      Pages: 5618-5628

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Development of A Non-viral Multifunctional Envelop-type Nano Device By A Novel Lipid Film Hydration Method.2004

    • Author(s)
      K.Kogure
    • Journal Title

      J.Cont.Rel. 98

      Pages: 317-323

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Factors governing the in vivo tissue uptake of transferrin coupled polyethylene glycol liposomes in vivo.2004

    • Author(s)
      H.Hatakeyama
    • Journal Title

      Int.J.Pharm. 281

      Pages: 25-33

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Quantitative three-dimesional analysis of the intracellular trafficking of plasmid DNA transfected by non-viral gene delivery system using con focal laser scanning microscopy.2004

    • Author(s)
      H.Akita
    • Journal Title

      Mol.Ther. 9

      Pages: 443-451

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Transferrin-modified liposomes equipped with pH-sensitive fusogenic peptide : An artificial viral-like delivery system.2004

    • Author(s)
      T.Kakudo
    • Journal Title

      Biochemstry 18

      Pages: 5618-5628

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] No enhancement of nuclear entry by direct conjugation of a nuclear localization signal peptide to linearized DNA.2003

    • Author(s)
      M.Tanimoto
    • Journal Title

      Bioconj.Chem. 14

      Pages: 1197-1202

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] An order of magnitude increased gene correction efficiency with sense single-stranded DNA fragment upon small fragment homologous replacement.

    • Author(s)
      H.Tsuchiya
    • Journal Title

      J.Gen.Med. (in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Construction of multifunctional envelope-type nano device by a SUV^*-fusion method.

    • Author(s)
      K.Sasaki
    • Journal Title

      Int.J.Pharm. (in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Evaluation of nuclear translocation and transcription of plasmid DNA condensed with protamine by microinjection : introduction of Nuclear Translocation score.

    • Author(s)
      T.Masuda
    • Journal Title

      FEBS Lett. (in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] An order of magnitude increased gene correction efficiency with sense single-stranded DNA fragment upon small fragment homologous replacement.

    • Author(s)
      H.Tsuchiya
    • Journal Title

      J.Gen.Med. (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Construction of multifunctional envelope-type nano device by a SUV^*-fusion method.

    • Author(s)
      K.Sasaki
    • Journal Title

      Int.J.Pharm. (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Evaluation of nuclear translocation and transcription of plasmid DNA condensed with, protamine by microinjection : introduction of Nuclear Translocation score.

    • Author(s)
      T.Masuda
    • Journal Title

      FEBS Lett. (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] An order of magnitude increased gene correction efficiency with sense single-stranded DNA fragment upon small fragment homologous replacement.

    • Author(s)
      H.Tsuchiya
    • Journal Title

      J.Gen.Med. (in press)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Construction of multifunctional envelope-type nano device by a SUV^*-fusion method.

    • Author(s)
      K.Sasaki
    • Journal Title

      Int.J.Pharm. (in press)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Evaluation of nuclear translocation and transcription of plasmid DNA condensed with protamine by microinjection : introduction of Nuclear Translocation score.

    • Author(s)
      T.Masuda
    • Journal Title

      FEBS Lett. (in press)

    • Related Report
      2004 Annual Research Report
  • [Patent(Industrial Property Rights)] リポソーム封入物質がエンドソームから脱出可能なリポソーム2004

    • Inventor(s)
      茶木 伸治, 小暮 健太朗, 二木 史朗, 原島 秀吉
    • Industrial Property Rights Holder
      科学技術振興機構
    • Industrial Property Number
      2004-205217
    • Filing Date
      2004-07-12
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Patent(Industrial Property Rights)] 核移行性を有する二枚膜構造遺伝子封入体の構築技術2004

    • Inventor(s)
      原島 秀吉
    • Filing Date
      2004-03-24
    • Related Report
      2004 Annual Research Report
  • [Patent(Industrial Property Rights)] 細胞に導入した核酸の標的オルガネラにおける局在量を定量する方法2003

    • Inventor(s)
      原島 秀吉, 秋田 英万
    • Industrial Property Rights Holder
      科学技術振興機構
    • Industrial Property Number
      2003-193038
    • Filing Date
      2003
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Patent(Industrial Property Rights)] 核移行能を有するポリアルギニン修飾リポソーム2003

    • Inventor(s)
      原島 秀吉, 二木 史朗, 小暮 健太朗
    • Industrial Property Rights Holder
      科学技術振興機構
    • Industrial Property Number
      2003-343857
    • Filing Date
      2003
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Patent(Industrial Property Rights)] SUV型リポソームの膜融合を利用した遺伝子等の新規封入技術

    • Inventor(s)
      原島 秀吉
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Patent(Industrial Property Rights)] 核移行性を有する二枚膜構造遺伝子封入体の構築技術

    • Inventor(s)
      原島 秀吉
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Publications] M.R.Almofti: "Cationic liposome-mediated gene delivery : Biophysical study and mechanism of internalization"Archives Biochem.Biophys. 410. 246-253 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] M.R.Almofti: "Lipoplex size determines lipofection efficiency with or without serum"Mol.Mem.Biol.. 20. 35-43 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] M.Tanimoto: "No enhancement of nuclear entry by direct conjugation of a nuclear localization signal peptide to linearized DNA"Bioconj.Chem.. 14. 1197-1202 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] I.A.Khalil: "Mechanism of improvedgene transfer by the N-terminal stearylation of octaarginine : Enhanced cellular association by hydrophobic core formation"Gene Therapy. 11. 636-644 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] R.Tachibana: "An assessment of relative transcriptional availability from nonviral vectors"Int J Pharm.. 270. 315-321 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] H.Akita: "Quantitative three-dimesional analysis of theintracellular trafficking of plasmid DNA transfected by non-viral gene delivery system using confocal laser"Mol.Ther.. 9. 443-451 (2004)

    • Related Report
      2003 Annual Research Report

URL: 

Published: 2003-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi