| Project/Area Number |
15300172
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | Kansai University |
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Principal Investigator |
OHYA Yuichi Kansai University, Faculty of Engineering, Associate Professor, 工学部, 助教授 (10213886)
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| Co-Investigator(Kenkyū-buntansha) |
OUCHI Tatsuro Kansai University, Faculty of Engineering, Professor, 工学部, 教授 (60067650)
日本油脂株式会社, 筑波研究所, 研究員
TANAKA Shinji NOF Corporation, Researcher
田中 信二 日本油脂株式会社, 筑波研究所, 研究員
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| Project Period (FY) |
2003 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥15,300,000 (Direct Cost: ¥15,300,000)
Fiscal Year 2005: ¥4,300,000 (Direct Cost: ¥4,300,000)
Fiscal Year 2004: ¥4,600,000 (Direct Cost: ¥4,600,000)
Fiscal Year 2003: ¥6,400,000 (Direct Cost: ¥6,400,000)
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| Keywords | Poly(lactic acid) / Polydepsipeptide / Poly(ethylene glycol) / Anti-adhesive membrane / Biodegradable materials / Biocompatibility / Bioabsrobability / Block copolymers |
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| Research Abstract |
In order to develop material for bioabsorbable anti-adhesive membrane, amphiphilic ABA-type triblock copolymers were synthesized. A segment is poly[L-lactide(LA)-r-depsipeptide] (poly[LA-r-(Glc-Leu)] : PLGL or poly[LA-r-(Glc-Phe)] : PLGF) and B segment is poly(ethylene glycol) (PEG) (Mn=10,000 and Mn=20,500). The synthesis of the triblock copolymer (PLGL-PEG-PLGL and PLGF-PEG-PLGF) was carried out via ring-opening copolymerization of L-lactide and cyclo(Glc-Leu) or cyclo(Glc-Phe) in the presence of hydroxytelechelic poly(ethylene glycol) using tin 2-ethylhexanoate as a catalyst. In this study, to evaluate the copolymers as a candidate for bioabsorbable anti-adhesive membrane, physicochemical properties such as degradation behavior under physiological condition and water absorption behavior of PLGL-PEG-PLGL and PLGF-PEG-PLGF films were investigated and compared with polylactide-PEG-polylactide (PLLA-PEG-PLLA) triblock copolymer. The PLGF-PEG-PLGF films showed higher Young's modulus and longer elongation to break than PLGL-PEG-PLGL films and PLLA-PEG-PLLA. The degradation rate of PLGL-PEG-PLGL and PLGF-PEG-PLGF films could be varied by controlling the molecular architectures, such as molecular weight of hydrophilic B segment and depsipeptide unit content in A segment. The degradation rate of PLGF-PEG-PLGF films was slightly higher than that of PLGL-PEG-PLGL films having similar molecular weight and content of depsipeptide unit. The biocompatibility and absorption behavior of the PLGL-PEG-PLGL films were also evaluated in vivo. The films were implanted subcutaneously into ddy-mice. The results showed that the PLGL-PEG-PLGL films were absorbed gradually in vivo without inflammation.
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